JPH0573760B2 - - Google Patents
Info
- Publication number
- JPH0573760B2 JPH0573760B2 JP59032199A JP3219984A JPH0573760B2 JP H0573760 B2 JPH0573760 B2 JP H0573760B2 JP 59032199 A JP59032199 A JP 59032199A JP 3219984 A JP3219984 A JP 3219984A JP H0573760 B2 JPH0573760 B2 JP H0573760B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- tumor
- dissolved
- activity
- vascular endothelial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000000259 anti-tumor effect Effects 0.000 claims description 12
- 210000000845 cartilage Anatomy 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 7
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000003125 aqueous solvent Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims 1
- 239000000523 sample Substances 0.000 description 31
- 206010028980 Neoplasm Diseases 0.000 description 21
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 16
- 230000002401 inhibitory effect Effects 0.000 description 12
- 210000003556 vascular endothelial cell Anatomy 0.000 description 12
- 239000002504 physiological saline solution Substances 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 230000005764 inhibitory process Effects 0.000 description 9
- 230000004663 cell proliferation Effects 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 8
- 230000006820 DNA synthesis Effects 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 239000012528 membrane Substances 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 241000283690 Bos taurus Species 0.000 description 6
- 230000001605 fetal effect Effects 0.000 description 6
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- SLXKOJJOQWFEFD-UHFFFAOYSA-N 6-aminohexanoic acid Chemical compound NCCCCCC(O)=O SLXKOJJOQWFEFD-UHFFFAOYSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 4
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 229960000789 guanidine hydrochloride Drugs 0.000 description 4
- 238000002513 implantation Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 210000004881 tumor cell Anatomy 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000013076 target substance Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 208000001382 Experimental Melanoma Diseases 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 239000003855 balanced salt solution Substances 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 210000002235 sarcomere Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- -1 As a control group Substances 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 229920002271 DEAE-Sepharose Polymers 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 150000002357 guanidines Chemical class 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 210000001147 pulmonary artery Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59032199A JPS60178820A (ja) | 1984-02-22 | 1984-02-22 | 抗腫瘍活性物質の製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59032199A JPS60178820A (ja) | 1984-02-22 | 1984-02-22 | 抗腫瘍活性物質の製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60178820A JPS60178820A (ja) | 1985-09-12 |
| JPH0573760B2 true JPH0573760B2 (enExample) | 1993-10-15 |
Family
ID=12352232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59032199A Granted JPS60178820A (ja) | 1984-02-22 | 1984-02-22 | 抗腫瘍活性物質の製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60178820A (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5242692A (en) * | 1990-07-10 | 1993-09-07 | Bar Ilan University | Anti-metastatic factor |
| US6380366B1 (en) | 1994-04-28 | 2002-04-30 | Les Laboratoires Aeterna Inc. | Shark cartilage extract:process of making, methods of using and compositions thereof |
| ATE241993T1 (de) | 1997-03-11 | 2003-06-15 | Aeterna Lab Inc | Zubereitungen zur tumorbehandlung enthaltend hai- knorpelextrakte und antineoplastische mittel |
| US6168807B1 (en) | 1998-07-23 | 2001-01-02 | Les Laboratoires Aeterna Inc. | Low molecular weight components of shark cartilage, processes for their preparation and therapeutic uses thereof |
-
1984
- 1984-02-22 JP JP59032199A patent/JPS60178820A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60178820A (ja) | 1985-09-12 |
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