JPH0570640B2 - - Google Patents
Info
- Publication number
- JPH0570640B2 JPH0570640B2 JP60118773A JP11877385A JPH0570640B2 JP H0570640 B2 JPH0570640 B2 JP H0570640B2 JP 60118773 A JP60118773 A JP 60118773A JP 11877385 A JP11877385 A JP 11877385A JP H0570640 B2 JPH0570640 B2 JP H0570640B2
- Authority
- JP
- Japan
- Prior art keywords
- killer
- factor
- medium
- salt
- spectrum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000003839 salts Chemical class 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 230000031942 natural killer cell mediated cytotoxicity Effects 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 230000001665 lethal effect Effects 0.000 claims description 7
- 238000000862 absorption spectrum Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 4
- 238000001228 spectrum Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 238000002523 gelfiltration Methods 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 claims description 3
- 241000228212 Aspergillus Species 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 241000235648 Pichia Species 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 2
- 238000000921 elemental analysis Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 235000019834 papain Nutrition 0.000 claims description 2
- 229940055729 papain Drugs 0.000 claims description 2
- 235000019419 proteases Nutrition 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 239000002609 medium Substances 0.000 description 16
- 244000286779 Hansenula anomala Species 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 239000007218 ym medium Substances 0.000 description 6
- 235000014683 Hansenula anomala Nutrition 0.000 description 5
- 229920005654 Sephadex Polymers 0.000 description 4
- 239000012507 Sephadex™ Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000005342 ion exchange Methods 0.000 description 3
- 235000013555 soy sauce Nutrition 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101000574013 Homo sapiens Pre-mRNA-processing factor 40 homolog A Proteins 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- 102100025822 Pre-mRNA-processing factor 40 homolog A Human genes 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- -1 etc. Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Compounds Of Unknown Constitution (AREA)
- Soy Sauces And Products Related Thereto (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60118773A JPS62285A (ja) | 1985-06-03 | 1985-06-03 | キラ−因子Ｋｈ−２及びその製造法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60118773A JPS62285A (ja) | 1985-06-03 | 1985-06-03 | キラ−因子Ｋｈ−２及びその製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62285A JPS62285A (ja) | 1987-01-06 |
| JPH0570640B2 true JPH0570640B2 (enrdf_load_stackoverflow) | 1993-10-05 |
Family
ID=14744716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60118773A Granted JPS62285A (ja) | 1985-06-03 | 1985-06-03 | キラ−因子Ｋｈ−２及びその製造法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62285A (enrdf_load_stackoverflow) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59204804A (ja) * | 1983-05-09 | 1984-11-20 | Canon Inc | カラ−フイルタ− |
| CN1100848C (zh) * | 1997-05-23 | 2003-02-05 | 中国科学院金属腐蚀与防护研究所 | 一种报废导热油的再生方法 |
-
1985
- 1985-06-03 JP JP60118773A patent/JPS62285A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62285A (ja) | 1987-01-06 |
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