JPH0531744B2 - - Google Patents
Info
- Publication number
- JPH0531744B2 JPH0531744B2 JP28161684A JP28161684A JPH0531744B2 JP H0531744 B2 JPH0531744 B2 JP H0531744B2 JP 28161684 A JP28161684 A JP 28161684A JP 28161684 A JP28161684 A JP 28161684A JP H0531744 B2 JPH0531744 B2 JP H0531744B2
- Authority
- JP
- Japan
- Prior art keywords
- endotoxin
- time
- amount
- gelation
- transmitted light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002158 endotoxin Substances 0.000 claims description 71
- 238000001879 gelation Methods 0.000 claims description 55
- 238000000034 method Methods 0.000 claims description 38
- 239000000523 sample Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 25
- 238000005259 measurement Methods 0.000 claims description 24
- 238000012360 testing method Methods 0.000 claims description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims description 16
- 239000012085 test solution Substances 0.000 claims description 15
- 239000012488 sample solution Substances 0.000 claims description 13
- 230000007423 decrease Effects 0.000 claims description 7
- 238000011088 calibration curve Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000012480 LAL reagent Substances 0.000 description 16
- 230000000007 visual effect Effects 0.000 description 16
- 230000035945 sensitivity Effects 0.000 description 14
- 238000000691 measurement method Methods 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 5
- 230000035484 reaction time Effects 0.000 description 5
- 238000004737 colorimetric analysis Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 230000002123 temporal effect Effects 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000239218 Limulus Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 108010072542 endotoxin binding proteins Proteins 0.000 description 1
- 239000012525 endotoxin sample Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 239000002510 pyrogen Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- -1 transfusions Substances 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
- G01N21/83—Turbidimetric titration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0407—Batch operation; multisample devices with multiple optical units, e.g. one per sample
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28161684A JPS61159162A (ja) | 1984-12-28 | 1984-12-28 | エンドトキシンの測定方法 |
| EP89114580A EP0347951B1 (en) | 1984-06-27 | 1985-06-25 | Apparatus for measuring endotoxin |
| DE8585107877T DE3586075D1 (de) | 1984-06-27 | 1985-06-25 | Verfahren zur bestimmung von endotoxin. |
| AT85107877T ATE76507T1 (de) | 1984-06-27 | 1985-06-25 | Verfahren zur bestimmung von endotoxin. |
| DE89114580T DE3587510T2 (de) | 1984-06-27 | 1985-06-25 | Gerät zur Messung von Endotoxin. |
| AT89114580T ATE92630T1 (de) | 1984-06-27 | 1985-06-25 | Geraet zur messung von endotoxin. |
| EP85107877A EP0173021B1 (en) | 1984-06-27 | 1985-06-25 | Process for measuring endotoxin |
| US06/748,805 US4740460A (en) | 1984-06-27 | 1985-06-26 | Process for measuring endotoxin and apparatus used therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28161684A JPS61159162A (ja) | 1984-12-28 | 1984-12-28 | エンドトキシンの測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61159162A JPS61159162A (ja) | 1986-07-18 |
| JPH0531744B2 true JPH0531744B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1993-05-13 |
Family
ID=17641611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP28161684A Granted JPS61159162A (ja) | 1984-06-27 | 1984-12-28 | エンドトキシンの測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61159162A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4936682A (en) * | 1987-08-11 | 1990-06-26 | Associates Of Cape Cod, Inc. | Instrument for independently and kinetically measuring light transpassion through a plurality of samples |
| WO1995014932A1 (fr) * | 1993-11-22 | 1995-06-01 | Seikagaku Corporation | Procede de dosage d'une substance reagissant avec un reactif de limulus |
| US20100129260A1 (en) * | 2007-05-01 | 2010-05-27 | Yoshiaki Shirasawa | Gelation measuring apparatus and sample cell |
| US8462340B2 (en) | 2008-03-19 | 2013-06-11 | Toru Obata | Gel particle measuring apparatus |
| JP5312834B2 (ja) * | 2008-03-31 | 2013-10-09 | シスメックス株式会社 | 血液凝固分析装置、血液凝固分析方法、及び、コンピュータプログラム |
| CN101983338B (zh) | 2008-03-31 | 2015-03-04 | 希森美康株式会社 | 凝血分析仪、凝血分析方法及计算机程序 |
| JP5489680B2 (ja) * | 2009-12-02 | 2014-05-14 | 興和株式会社 | 生物由来の生理活性物質の測定方法、それを実行するためのプログラム及び、生物由来の生理活性物質の測定装置 |
| JP5421622B2 (ja) * | 2009-03-13 | 2014-02-19 | 興和株式会社 | 生物由来の生理活性物質の測定方法及び測定装置 |
| CN102348984B (zh) * | 2009-03-13 | 2014-04-02 | 兴和株式会社 | 来自生物的生理活性物质的测定方法以及测定装置 |
-
1984
- 1984-12-28 JP JP28161684A patent/JPS61159162A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61159162A (ja) | 1986-07-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0173021B1 (en) | Process for measuring endotoxin | |
| JPH0531744B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| JPWO2008139544A1 (ja) | ゲル化測定装置および試料セル | |
| JP2001249134A5 (ja) | タンパク質濃度計測方法 | |
| AU2001247614B2 (en) | Lithium ion-selective electrode for clinical applications | |
| AU2001247614A1 (en) | Lithium ion-selective electrode for clinical applications | |
| CA1144461A (en) | Chromogenic detection of endotoxin in human serum and plasma | |
| US3531254A (en) | Test article and method for the detection of urea | |
| JP2004212120A (ja) | 測定値推定機能を持つ測定装置及び測定方法 | |
| JP5401115B2 (ja) | 生物由来の生理活性物質の測定方法及び測定装置 | |
| JPS6193958A (ja) | エンドトキシンの定量法 | |
| JP3061418B2 (ja) | リムルス試薬反応性物質の測定方法 | |
| Kohn et al. | An immunological method for the detection and estimation of fetal haemoglobin | |
| US4992382A (en) | Porous polymer film calcium ion chemical sensor and method of using the same | |
| JPH07113643B2 (ja) | エンドトキシンの新規測定方法 | |
| CN1715925A (zh) | 一种通过测定浊度值来进行细菌内毒素检查的方法 | |
| JP2639019B2 (ja) | エンドトキシンの定量方法 | |
| Yoshikazu et al. | A simple and rapid microdiffusion method for blood ammonia using a reflectance meter and a reagent plate, and its clinical evaluation for liver diseases | |
| EP0731353A1 (en) | Method of assaying limulus reagent-reactive substance | |
| CA1060323A (en) | Limulus lysate turbidity test for pyrogens | |
| SU1233047A1 (ru) | Способ определени осми | |
| CN119936392A (zh) | Tat复合物测定方法、封闭抗体、检测抗体及试剂盒 | |
| CN115308141A (zh) | 一种腺苷脱氨酶（ada）检测试剂盒质量检测方法 | |
| Pannhorst et al. | Simple method for immobilization and characterization of cationic indicators on an optically clear polystyrene surface | |
| JPH0265799A (ja) | 細胞内の物質測定方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |