JPH038196B2 - - Google Patents
Info
- Publication number
- JPH038196B2 JPH038196B2 JP29549785A JP29549785A JPH038196B2 JP H038196 B2 JPH038196 B2 JP H038196B2 JP 29549785 A JP29549785 A JP 29549785A JP 29549785 A JP29549785 A JP 29549785A JP H038196 B2 JPH038196 B2 JP H038196B2
- Authority
- JP
- Japan
- Prior art keywords
- padiase
- adsorbent
- present
- solution
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 claims description 27
- 102000004169 proteins and genes Human genes 0.000 claims description 20
- 108090000623 proteins and genes Proteins 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 16
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 15
- 239000003463 adsorbent Substances 0.000 claims description 15
- 229910001424 calcium ion Inorganic materials 0.000 claims description 15
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- 239000002738 chelating agent Substances 0.000 claims description 7
- 239000002753 trypsin inhibitor Substances 0.000 claims description 6
- 101710162629 Trypsin inhibitor Proteins 0.000 claims description 5
- 229940122618 Trypsin inhibitor Drugs 0.000 claims description 5
- 239000013522 chelant Substances 0.000 claims description 3
- 235000018102 proteins Nutrition 0.000 description 17
- 238000000746 purification Methods 0.000 description 16
- 102000004190 Enzymes Human genes 0.000 description 15
- 108090000790 Enzymes Proteins 0.000 description 15
- 230000000694 effects Effects 0.000 description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- -1 DEAE-cephadex Chemical compound 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 210000002027 skeletal muscle Anatomy 0.000 description 5
- 241000283690 Bos taurus Species 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 238000001042 affinity chromatography Methods 0.000 description 4
- 210000004556 brain Anatomy 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000005571 anion exchange chromatography Methods 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000004440 column chromatography Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 229950007919 egtazic acid Drugs 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 238000011403 purification operation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XNCSCQSQSGDGES-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]propyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)C(C)CN(CC(O)=O)CC(O)=O XNCSCQSQSGDGES-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical group CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- 244000291564 Allium cepa Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 101000585591 Bos taurus Pancreatic prohormone Proteins 0.000 description 1
- 101000598319 Bos taurus Serine protease inhibitor Kazal-type 1 Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical compound NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 description 1
- RHGKLRLOHDJJDR-UHFFFAOYSA-N Ndelta-carbamoyl-DL-ornithine Natural products OC(=O)C(N)CCCNC(N)=O RHGKLRLOHDJJDR-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 1
- 229910001413 alkali metal ion Inorganic materials 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 239000003715 calcium chelating agent Substances 0.000 description 1
- 229940043430 calcium compound Drugs 0.000 description 1
- 150000001674 calcium compounds Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000006329 citrullination Effects 0.000 description 1
- 229960002173 citrulline Drugs 0.000 description 1
- 235000013477 citrulline Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000001235 protein arginine deiminase Human genes 0.000 description 1
- 108060006632 protein arginine deiminase Proteins 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP29549785A JPS62155088A (ja) | 1985-12-26 | 1985-12-26 | タンパク質アルギニン残基脱イミノ酵素の精製法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP29549785A JPS62155088A (ja) | 1985-12-26 | 1985-12-26 | タンパク質アルギニン残基脱イミノ酵素の精製法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS62155088A JPS62155088A (ja) | 1987-07-10 |
JPH038196B2 true JPH038196B2 (de) | 1991-02-05 |
Family
ID=17821374
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP29549785A Granted JPS62155088A (ja) | 1985-12-26 | 1985-12-26 | タンパク質アルギニン残基脱イミノ酵素の精製法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS62155088A (de) |
-
1985
- 1985-12-26 JP JP29549785A patent/JPS62155088A/ja active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS62155088A (ja) | 1987-07-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Aronsson et al. | Characterization of glyoxalase I purified from pig erythrocytes by affinity chromatography | |
JPH05227962A (ja) | ウイルス安全性精製ヒトトロンビン | |
JPH04211372A (ja) | 改良されたトロンビンの製造方法およびそれにより得られた高純度トロンビン調製物 | |
US4259447A (en) | Process for the production of urokinase in pure condition | |
US4380511A (en) | Purification of bovine thrombin | |
US5831025A (en) | Human activated protein C and process for preparing same | |
US4189350A (en) | Process for purifying urokinase | |
JPH038196B2 (de) | ||
US4160697A (en) | Method for purification of crude urokinase | |
WO1988001294A1 (en) | Process for separating single-strand tpa and double-strand tpa from each other | |
EP0358274B1 (de) | Verfahren zum Reinigen von Gewebe-Plasminogenaktivator | |
JPH0147997B2 (de) | ||
JP2882828B2 (ja) | 人尿トリプシンインヒビターの精製方法 | |
JPH0260316B2 (de) | ||
KR830001822B1 (ko) | 유로 키나제의 제조방법 | |
KR100392779B1 (ko) | 고정화 아미노펩티다제를 이용한천연형 재조합단백질의 제조방법 | |
JP2714817B2 (ja) | ウロキナーゼ前駆体の製造方法 | |
JPH04365481A (ja) | トロンビンの製造法 | |
JPH0779692B2 (ja) | 一本鎖t−PAと二本鎖t−PAを分離する方法 | |
KR790001535B1 (ko) | 유로키나제의 추출방법 | |
JPH04126092A (ja) | 大腸菌由来蛋白の除去方法 | |
KR790001376B1 (ko) | 우로키나제의 정제 및 회수법 | |
JPS5830034B2 (ja) | カリクレインの精製法 | |
JPS5838150B2 (ja) | カリクレインの精製法 | |
JP2004244348A (ja) | パプトグロビンの精製法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
EXPY | Cancellation because of completion of term |