JPH0379995B2 - - Google Patents
Info
- Publication number
- JPH0379995B2 JPH0379995B2 JP62056050A JP5605087A JPH0379995B2 JP H0379995 B2 JPH0379995 B2 JP H0379995B2 JP 62056050 A JP62056050 A JP 62056050A JP 5605087 A JP5605087 A JP 5605087A JP H0379995 B2 JPH0379995 B2 JP H0379995B2
- Authority
- JP
- Japan
- Prior art keywords
- monoclonal antibody
- antibody
- pseudourisin
- culture supernatant
- elisa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 18
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 17
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 claims description 9
- 229940035893 uracil Drugs 0.000 claims description 9
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 claims description 9
- 229940045145 uridine Drugs 0.000 claims description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 8
- 229940098773 bovine serum albumin Drugs 0.000 claims description 8
- 238000002965 ELISA Methods 0.000 claims description 7
- 230000009260 cross reactivity Effects 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 7
- 239000012895 dilution Substances 0.000 claims description 7
- 238000002835 absorbance Methods 0.000 claims description 5
- 239000012228 culture supernatant Substances 0.000 claims description 5
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 claims description 3
- 229930185560 Pseudouridine Natural products 0.000 claims description 3
- PTJWIQPHWPFNBW-UHFFFAOYSA-N Pseudouridine C Natural products OC1C(O)C(CO)OC1C1=CNC(=O)NC1=O PTJWIQPHWPFNBW-UHFFFAOYSA-N 0.000 claims description 3
- WGDUUQDYDIIBKT-UHFFFAOYSA-N beta-Pseudouridine Natural products OC1OC(CN2C=CC(=O)NC2=O)C(O)C1O WGDUUQDYDIIBKT-UHFFFAOYSA-N 0.000 claims description 3
- 229960002949 fluorouracil Drugs 0.000 claims description 3
- PTJWIQPHWPFNBW-GBNDHIKLSA-N pseudouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1C1=CNC(=O)NC1=O PTJWIQPHWPFNBW-GBNDHIKLSA-N 0.000 claims description 3
- 201000011510 cancer Diseases 0.000 description 14
- 210000004408 hybridoma Anatomy 0.000 description 12
- 206010028980 Neoplasm Diseases 0.000 description 9
- 102000014914 Carrier Proteins Human genes 0.000 description 8
- 108010078791 Carrier Proteins Proteins 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000002953 phosphate buffered saline Substances 0.000 description 5
- 210000002700 urine Anatomy 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 239000002671 adjuvant Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 230000002163 immunogen Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 210000004988 splenocyte Anatomy 0.000 description 3
- 102000012406 Carcinoembryonic Antigen Human genes 0.000 description 2
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000006957 competitive inhibition Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 210000000628 antibody-producing cell Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 108060003552 hemocyanin Proteins 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62056050A JPS63222699A (ja) | 1987-03-11 | 1987-03-11 | 単クローン性抗体及びこれを用いるシユードウリジンψの測定法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62056050A JPS63222699A (ja) | 1987-03-11 | 1987-03-11 | 単クローン性抗体及びこれを用いるシユードウリジンψの測定法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63222699A JPS63222699A (ja) | 1988-09-16 |
| JPH0379995B2 true JPH0379995B2 (enrdf_load_stackoverflow) | 1991-12-20 |
Family
ID=13016255
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62056050A Granted JPS63222699A (ja) | 1987-03-11 | 1987-03-11 | 単クローン性抗体及びこれを用いるシユードウリジンψの測定法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63222699A (enrdf_load_stackoverflow) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990010708A1 (fr) * | 1989-03-09 | 1990-09-20 | The Sendai Institute Of Microbiology | Anticorps monoclonal, et procede d'analyse, kit de reactif, procede de recherche et medicament utilisant ledit anticorps |
| US5198367A (en) * | 1989-06-09 | 1993-03-30 | Masuo Aizawa | Homogeneous amperometric immunoassay |
| JP3686977B2 (ja) * | 1997-10-16 | 2005-08-24 | 源一郎 杣 | 抗ウラシルモノクローナル抗体及びそれを産生するハイブリドーマ |
| US20060177883A1 (en) | 2005-02-08 | 2006-08-10 | Saladax Biomedical Inc. | 5-Fluoro-uracil immunoassay |
-
1987
- 1987-03-11 JP JP62056050A patent/JPS63222699A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63222699A (ja) | 1988-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5767247A (en) | Anti-annexin-V monoclonal antibodies, and preparation and use thereof | |
| JPH0471516B2 (enrdf_load_stackoverflow) | ||
| CN113736744B (zh) | 洋地黄毒苷单克隆抗体杂交瘤细胞株及其应用 | |
| JPH0379995B2 (enrdf_load_stackoverflow) | ||
| JPH0421479B2 (enrdf_load_stackoverflow) | ||
| US20040142400A1 (en) | High affinity monoclonal antibody for recognizing the estrogen receptor (ER) and method for creating the antibody | |
| EP0328578B1 (en) | Antigen recognized by mca 16-88 | |
| JPH0379994B2 (enrdf_load_stackoverflow) | ||
| JP4663831B2 (ja) | モノクローナル抗体、細胞株及びｎ１，ｎ１２−ジアセチルスペルミンの測定法 | |
| JP2888587B2 (ja) | 癌関連ヒト由来ガラクトース転移酵素に特異的なモノクローナル抗体、それを産生するハイブリドーマ及びそれを用いた検体中の癌関連ヒト由来ガラクトース転移酵素の測定方法 | |
| JPH0753756B2 (ja) | 抗ヘパラン硫酸モノクロ−ン抗体及びこれを用いるヘパラン硫酸の検出方法 | |
| JP2896931B2 (ja) | モノクローナル抗体、それを用いた測定法、試薬キット、検索法及び薬剤ミサイル | |
| JPH0782015B2 (ja) | Ｄ型バニリルマンデル酸の測定法およびそれに使用する試薬およびキット | |
| CN116396392B (zh) | 一种特异性针对异羟基洋地黄毒甙元的抗体及其相关应用 | |
| JPS63209596A (ja) | モノクロ−ナル抗体及びその使用方法 | |
| EP0433452A1 (en) | Monoclonal antibody, and assay method, reagent kit, search method and drug missile using said antibody | |
| CN115385999A (zh) | 一种腺苷人工抗原、腺苷抗体及其应用 | |
| JP2743015B2 (ja) | Ｏ―アセチル化されたガングリオシドｇｍ▲下３▼に特異的なモノクローナル抗体、該抗体を産生するハイブリドーマ及びその作製方法 | |
| JPH11507014A (ja) | 標識として用いる酵素の反応生成物に向けられた抗体を使用する免疫学的検定 | |
| JP3849001B2 (ja) | 抗−２′−デオキシシチジン抗体並びにその製造及び使用 | |
| JP2004091454A (ja) | 抗体及びその製造方法並びに抗体を用いた抗原の定量方法 | |
| JP3264890B2 (ja) | 抗体及びその製造方法並びに抗体を用いた抗原の定量方法及び免疫染色方法 | |
| JPH11318448A (ja) | 抗原、抗体およびそれを用いる免疫学的測定方法 | |
| JPH0549495A (ja) | モノクローナル抗体及びヒト血漿α（１→３）フコース転移酵素の定量方法 | |
| JPH031958B2 (enrdf_load_stackoverflow) |