JPH0367224B2 - - Google Patents
Info
- Publication number
- JPH0367224B2 JPH0367224B2 JP58189162A JP18916283A JPH0367224B2 JP H0367224 B2 JPH0367224 B2 JP H0367224B2 JP 58189162 A JP58189162 A JP 58189162A JP 18916283 A JP18916283 A JP 18916283A JP H0367224 B2 JPH0367224 B2 JP H0367224B2
- Authority
- JP
- Japan
- Prior art keywords
- light
- microparticles
- particles
- time
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002245 particle Substances 0.000 claims description 56
- 239000011859 microparticle Substances 0.000 claims description 52
- 238000000034 method Methods 0.000 claims description 49
- 230000008859 change Effects 0.000 claims description 29
- 230000002123 temporal effect Effects 0.000 claims description 19
- 238000000295 emission spectrum Methods 0.000 claims description 16
- 238000001514 detection method Methods 0.000 claims description 14
- 230000005684 electric field Effects 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 7
- 230000001678 irradiating effect Effects 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 description 94
- 239000000975 dye Substances 0.000 description 25
- 230000002159 abnormal effect Effects 0.000 description 12
- 238000010586 diagram Methods 0.000 description 11
- 230000005284 excitation Effects 0.000 description 11
- 230000003287 optical effect Effects 0.000 description 11
- 238000001228 spectrum Methods 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 10
- 238000005259 measurement Methods 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- 238000010521 absorption reaction Methods 0.000 description 8
- 230000010287 polarization Effects 0.000 description 8
- 239000007788 liquid Substances 0.000 description 5
- 230000001360 synchronised effect Effects 0.000 description 5
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 4
- 229910052786 argon Inorganic materials 0.000 description 4
- -1 argon ion Chemical class 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 230000005856 abnormality Effects 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 230000003111 delayed effect Effects 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- 238000004020 luminiscence type Methods 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 239000000999 acridine dye Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000003211 malignant effect Effects 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 102000018656 Mitogen Receptors Human genes 0.000 description 1
- 108010052006 Mitogen Receptors Proteins 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010047571 Visual impairment Diseases 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000006552 photochemical reaction Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP58189162A JPS6080764A (ja) | 1983-10-12 | 1983-10-12 | 埮å°ç²åãèå¥ããæ¹æ³åã³ãã®è£ 眮 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP58189162A JPS6080764A (ja) | 1983-10-12 | 1983-10-12 | 埮å°ç²åãèå¥ããæ¹æ³åã³ãã®è£ 眮 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6080764A JPS6080764A (ja) | 1985-05-08 |
JPH0367224B2 true JPH0367224B2 (ja) | 1991-10-22 |
Family
ID=16236491
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP58189162A Granted JPS6080764A (ja) | 1983-10-12 | 1983-10-12 | 埮å°ç²åãèå¥ããæ¹æ³åã³ãã®è£ 眮 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6080764A (ja) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6135331A (ja) * | 1984-07-28 | 1986-02-19 | Japan Spectroscopic Co | 埮å°ç²ååé¢è£ 眮 |
JPH073419B2 (ja) * | 1986-10-07 | 1995-01-18 | æ±äºå»çšé»åæ ªåŒäŒç€Ÿ | æµäœäžã®çŽ°èåææ¹æ³ããã³è£ 眮 |
JPH01209343A (ja) * | 1988-02-16 | 1989-08-23 | Shimadzu Corp | åå èå å åºŠèš |
JP4300366B2 (ja) * | 2005-03-30 | 2009-07-22 | ç¬ç«è¡æ¿æ³äººç£æ¥æè¡ç·åç 究æ | èå æ€åºæ¹æ³ãèå æ€åºçšããŒãºã®äœè£œæ¹æ³åã³èå æ€åºçšããŒãº |
JP4399575B2 (ja) * | 2005-03-31 | 2010-01-20 | ç¬ç«è¡æ¿æ³äººç£æ¥æè¡ç·åç 究æ | éºäŒåæçäžã®ãã¯ã¬ãªããã®çš®é¡ã®ç¹å®æ¹æ³ |
JP5892967B2 (ja) * | 2013-03-29 | 2016-03-23 | ã·ã¹ã¡ãã¯ã¹æ ªåŒäŒç€Ÿ | 现èåæè£ çœ®ã现è枬å®è£ 眮ã®ç®¡çæ¹æ³ããã³ã³ã³ãã¥ãŒã¿ããã°ã©ã |
US10564088B2 (en) | 2014-10-09 | 2020-02-18 | Kinetic River Corp. | Particle analysis and sorting apparatus and methods |
US11740174B2 (en) | 2014-10-09 | 2023-08-29 | Kinetic River Corp. | Apparatus and methods for particle analysis and autofluorescence discrimination |
US9658148B2 (en) * | 2014-10-09 | 2017-05-23 | Kinetic River Corp. | Particle analysis and sorting apparatus and methods |
WO2017130707A1 (ja) | 2016-01-26 | 2017-08-03 | æ ªåŒäŒç€Ÿãªã³ãŒ | 液滎圢æè£ çœ®ãåæ³šè£ çœ® |
JP6915240B2 (ja) * | 2016-07-29 | 2021-08-04 | æ ªåŒäŒç€Ÿãªã³ãŒ | ç²åèšæ°è£ 眮åã³ç²åèšæ°æ¹æ³ã䞊ã³ã«æ¶²æ»Žåœ¢æè£ çœ®ãåã³åæ³šè£ çœ® |
JP6888289B2 (ja) * | 2016-12-12 | 2021-06-16 | æ ªåŒäŒç€Ÿãªã³ãŒ | 液滎圢æè£ çœ®ã液滎圢ææ¹æ³ãåã³åæ³šè£ çœ® |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS4874292A (ja) * | 1971-12-23 | 1973-10-06 | ||
JPS5190868A (ja) * | 1975-02-06 | 1976-08-09 | ||
JPS5633052A (en) * | 1979-08-20 | 1981-04-03 | Ortho Diagnostics | Electrostatic grain selecting method and its mechanism |
JPS57194354A (en) * | 1981-05-26 | 1982-11-29 | Fujitsu Ltd | Analytical separation apparatus of multistage type living body cell |
JPS57211557A (en) * | 1981-06-24 | 1982-12-25 | Fujitsu Ltd | Cell sorter |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6445018A (en) * | 1987-08-13 | 1989-02-17 | Univ Tokai | Manufacture of superconductive material |
-
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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JPS4874292A (ja) * | 1971-12-23 | 1973-10-06 | ||
JPS5190868A (ja) * | 1975-02-06 | 1976-08-09 | ||
JPS5633052A (en) * | 1979-08-20 | 1981-04-03 | Ortho Diagnostics | Electrostatic grain selecting method and its mechanism |
JPS57194354A (en) * | 1981-05-26 | 1982-11-29 | Fujitsu Ltd | Analytical separation apparatus of multistage type living body cell |
JPS57211557A (en) * | 1981-06-24 | 1982-12-25 | Fujitsu Ltd | Cell sorter |
Also Published As
Publication number | Publication date |
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JPS6080764A (ja) | 1985-05-08 |
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