JPH0354551B2 - - Google Patents
Info
- Publication number
- JPH0354551B2 JPH0354551B2 JP25421584A JP25421584A JPH0354551B2 JP H0354551 B2 JPH0354551 B2 JP H0354551B2 JP 25421584 A JP25421584 A JP 25421584A JP 25421584 A JP25421584 A JP 25421584A JP H0354551 B2 JPH0354551 B2 JP H0354551B2
- Authority
- JP
- Japan
- Prior art keywords
- phage
- cyclodextrin glucanotransferase
- plasmid
- strain
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108010025880 Cyclomaltodextrin glucanotransferase Proteins 0.000 claims description 23
- 239000013612 plasmid Substances 0.000 claims description 15
- 244000063299 Bacillus subtilis Species 0.000 claims description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 13
- 241000178960 Paenibacillus macerans Species 0.000 claims description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 14
- 239000013611 chromosomal DNA Substances 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 229930027917 kanamycin Natural products 0.000 description 8
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 8
- 229960000318 kanamycin Drugs 0.000 description 8
- 229930182823 kanamycin A Natural products 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 238000003776 cleavage reaction Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000007017 scission Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 210000000349 chromosome Anatomy 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- 241000701959 Escherichia virus Lambda Species 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000002298 density-gradient ultracentrifugation Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000004806 packaging method and process Methods 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 229920000858 Cyclodextrin Polymers 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000004816 paper chromatography Methods 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- MROZMRCKKCNECB-UHFFFAOYSA-N CC(=O)C.[I] Chemical compound CC(=O)C.[I] MROZMRCKKCNECB-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25421584A JPS61132178A (ja) | 1984-12-03 | 1984-12-03 | 変異バチルス・ズブチリス |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25421584A JPS61132178A (ja) | 1984-12-03 | 1984-12-03 | 変異バチルス・ズブチリス |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61132178A JPS61132178A (ja) | 1986-06-19 |
| JPH0354551B2 true JPH0354551B2 (lv) | 1991-08-20 |
Family
ID=17261857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP25421584A Granted JPS61132178A (ja) | 1984-12-03 | 1984-12-03 | 変異バチルス・ズブチリス |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61132178A (lv) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1335183C (en) * | 1984-12-03 | 1995-04-11 | Toshiyuki Sugimoto | Polypeptide possessing cyclomaltodextrin glucanotransferase activity |
| US5278059A (en) * | 1985-12-04 | 1994-01-11 | Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo | Polypeptide possessing cyclomaltodextrin glucanotransferase activity |
-
1984
- 1984-12-03 JP JP25421584A patent/JPS61132178A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61132178A (ja) | 1986-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH04507346A (ja) | アルカリ性タンパク質分解酵素およびその製造方法 | |
| US7582454B2 (en) | 5-substituted hydantoin racemase, DNA coding for the racemase, and processes for producing optically active amino acids | |
| JPH0354551B2 (lv) | ||
| JPS6374488A (ja) | ７−アミノセフアロスポラン酸およびその誘導体の製造法 | |
| JPH0587234B2 (lv) | ||
| EP0073436A2 (en) | Hybrid plasmid and preparation thereof | |
| US5049501A (en) | Production method for PvuI restriction endonuclease | |
| KR910005627B1 (ko) | 클론된 트립토판 합성효소 유전자 및 그를 함유하는 재조합 플라즈미드 | |
| JPH07108225B2 (ja) | Ｄ−アミノ酸オキシダ−ゼ遺伝子 | |
| JPH0355107B2 (lv) | ||
| JPS62262994A (ja) | Ｄ−アミノ酸オキシダ−ゼ遺伝子 | |
| JPH0533028B2 (lv) | ||
| JP4161236B2 (ja) | 新規なＬ−α−グリセロホスフェートオキシダーゼおよびその製造方法 | |
| JP2567199B2 (ja) | 枯草菌およびその形成方法 | |
| JPH0336514B2 (lv) | ||
| JPH07143880A (ja) | 超耐熱性α−アミラーゼ遺伝子 | |
| JPH11313683A (ja) | 新規キシロシダーゼ遺伝子、ベクター、それを用いた形質転換体及びその利用 | |
| JP2671008B2 (ja) | シクロマルトデキストリングルセノトランスフェラーゼをコードするｄｎａ，それを含む組換えプラスミド及びそのプラスミドを含む形質転換微生物 | |
| JP2000157279A (ja) | クレアチンアミジノハイドロラーゼ及びその製造法 | |
| JP3060019B2 (ja) | マルトテトラオース生成酵素遺伝子を含有するプラスミド及び該プラスミドを有する微生物並びに該微生物を用いるマルトテトラオース生成酵素の製造法 | |
| JPH02177886A (ja) | 乳酸オキシダーゼの遺伝情報を有するｄｎａおよびその用途 | |
| JP2593481B2 (ja) | サルコシンオキシダーゼの遺伝情報を有するｄｎａおよびその用途 | |
| JP3829950B2 (ja) | 新規なクレアチニンアミドヒドロラーゼ | |
| JPH0789912B2 (ja) | サイクロデキストリン グルカノトランスフェラーゼの製造法 | |
| KR20000069110A (ko) | 대장균에서 생성한 트리고노프시스 바리아빌리스의 변경 효소 ｄ |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |