JPH0336513B2 - - Google Patents

Info

Publication number
JPH0336513B2
JPH0336513B2 JP63193573A JP19357388A JPH0336513B2 JP H0336513 B2 JPH0336513 B2 JP H0336513B2 JP 63193573 A JP63193573 A JP 63193573A JP 19357388 A JP19357388 A JP 19357388A JP H0336513 B2 JPH0336513 B2 JP H0336513B2
Authority
JP
Japan
Prior art keywords
amount
formula
carbohydrate
dye
carbohydrates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63193573A
Other languages
English (en)
Japanese (ja)
Other versions
JPS6467187A (en
Inventor
Daburyu Matsukaatonii Henrii
Menharuto Yoozefu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of JPS6467187A publication Critical patent/JPS6467187A/ja
Publication of JPH0336513B2 publication Critical patent/JPH0336513B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Pressure Welding/Diffusion-Bonding (AREA)
  • Carbon And Carbon Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP63193573A 1987-08-05 1988-08-04 Method of separating and refining t-pa Granted JPS6467187A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873726019 DE3726019A1 (de) 1987-08-05 1987-08-05 Verfahren zur abtrennung und reinigung von t-pa

Publications (2)

Publication Number Publication Date
JPS6467187A JPS6467187A (en) 1989-03-13
JPH0336513B2 true JPH0336513B2 (en:Method) 1991-05-31

Family

ID=6333126

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63193573A Granted JPS6467187A (en) 1987-08-05 1988-08-04 Method of separating and refining t-pa

Country Status (4)

Country Link
EP (1) EP0302503B1 (en:Method)
JP (1) JPS6467187A (en:Method)
AT (1) ATE76300T1 (en:Method)
DE (2) DE3726019A1 (en:Method)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
JP2000286602A (ja) 1999-03-30 2000-10-13 Alps Electric Co Ltd 超広帯域直線・円偏波変換器
CN100335623C (zh) * 2005-10-20 2007-09-05 上海交通大学 纤溶酶原激活剂的仿生亲和纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH660742A5 (de) * 1982-10-29 1987-06-15 Mitsui Toatsu Chemicals Plasminogen-aktivator, verfahren zu seiner herstellung und diesen enthaltendes thrombolytisches agens.
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
DK175327B1 (da) * 1984-10-01 2004-08-23 Integrated Genetics Inc Replicerbar ekspressionsvektor og rekombinant DNA-molekyle, som indkoder aktiv human livmodervævsplasminogen-aktivator, pattedyrscelle transformeret med vektoren og fremgangsmåde til fremstilling af aktiv human vævsplasminogen-aktivator (TPA)
EP0236289A3 (en) * 1986-02-26 1988-12-07 Monsanto Company Tissue plasminogen activator from normal human colon cells

Also Published As

Publication number Publication date
DE3726019A1 (de) 1989-02-16
JPS6467187A (en) 1989-03-13
EP0302503B1 (de) 1992-05-20
ATE76300T1 (de) 1992-06-15
DE3871277D1 (de) 1992-06-25
EP0302503A3 (en) 1990-03-28
EP0302503A2 (de) 1989-02-08

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