JPH0314840B2 - - Google Patents
Info
- Publication number
- JPH0314840B2 JPH0314840B2 JP56061110A JP6111081A JPH0314840B2 JP H0314840 B2 JPH0314840 B2 JP H0314840B2 JP 56061110 A JP56061110 A JP 56061110A JP 6111081 A JP6111081 A JP 6111081A JP H0314840 B2 JPH0314840 B2 JP H0314840B2
- Authority
- JP
- Japan
- Prior art keywords
- gene
- dna
- hepatitis
- antigen
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000700721 Hepatitis B virus Species 0.000 claims description 71
- 239000012634 fragment Substances 0.000 claims description 53
- 239000000427 antigen Substances 0.000 claims description 45
- 102000036639 antigens Human genes 0.000 claims description 45
- 108091007433 antigens Proteins 0.000 claims description 45
- 101150010882 S gene Proteins 0.000 claims description 42
- 108090000623 proteins and genes Proteins 0.000 claims description 39
- 239000002245 particle Substances 0.000 claims description 36
- 239000013598 vector Substances 0.000 claims description 35
- 238000003780 insertion Methods 0.000 claims description 27
- 230000037431 insertion Effects 0.000 claims description 27
- 241000700605 Viruses Species 0.000 claims description 24
- 238000013518 transcription Methods 0.000 claims description 21
- 230000035897 transcription Effects 0.000 claims description 21
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 claims description 17
- 210000002966 serum Anatomy 0.000 claims description 16
- 229960005486 vaccine Drugs 0.000 claims description 13
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 11
- 208000006454 hepatitis Diseases 0.000 claims description 11
- 231100000283 hepatitis Toxicity 0.000 claims description 11
- 238000011144 upstream manufacturing Methods 0.000 claims description 9
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 108020004999 messenger RNA Proteins 0.000 claims description 6
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 claims description 5
- 230000002163 immunogen Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 230000004520 agglutination Effects 0.000 claims description 4
- 230000009466 transformation Effects 0.000 claims description 4
- 208000037319 Hepatitis infectious Diseases 0.000 claims description 3
- 239000003937 drug carrier Substances 0.000 claims description 3
- 208000005252 hepatitis A Diseases 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 claims description 2
- 230000006472 autoimmune response Effects 0.000 claims 2
- 238000010353 genetic engineering Methods 0.000 claims 2
- 230000036039 immunity Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 91
- 210000004027 cell Anatomy 0.000 description 50
- 239000013612 plasmid Substances 0.000 description 40
- 239000002609 medium Substances 0.000 description 24
- 238000000034 method Methods 0.000 description 20
- 102000004169 proteins and genes Human genes 0.000 description 17
- 239000002773 nucleotide Substances 0.000 description 16
- 125000003729 nucleotide group Chemical group 0.000 description 16
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 13
- 230000001900 immune effect Effects 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 10
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 108020004440 Thymidine kinase Proteins 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 8
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 7
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 7
- 229940104230 thymidine Drugs 0.000 description 7
- 108020004635 Complementary DNA Proteins 0.000 description 6
- 238000010804 cDNA synthesis Methods 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 102000004506 Blood Proteins Human genes 0.000 description 5
- 108010017384 Blood Proteins Proteins 0.000 description 5
- 108020004638 Circular DNA Proteins 0.000 description 5
- 206010019799 Hepatitis viral Diseases 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 238000003127 radioimmunoassay Methods 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 4
- 238000010166 immunofluorescence Methods 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 201000001862 viral hepatitis Diseases 0.000 description 4
- 230000003612 virological effect Effects 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- 101150111062 C gene Proteins 0.000 description 3
- 101710132601 Capsid protein Proteins 0.000 description 3
- 108010042407 Endonucleases Proteins 0.000 description 3
- 102000004533 Endonucleases Human genes 0.000 description 3
- 101710142246 External core antigen Proteins 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 239000004098 Tetracycline Substances 0.000 description 3
- 102000006601 Thymidine Kinase Human genes 0.000 description 3
- 108010003533 Viral Envelope Proteins Proteins 0.000 description 3
- 230000000890 antigenic effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 108091092356 cellular DNA Proteins 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 208000002672 hepatitis B Diseases 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229960002180 tetracycline Drugs 0.000 description 3
- 229930101283 tetracycline Natural products 0.000 description 3
- 235000019364 tetracycline Nutrition 0.000 description 3
- 150000003522 tetracyclines Chemical class 0.000 description 3
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 108020005202 Viral DNA Proteins 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 229960003896 aminopterin Drugs 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000012798 spherical particle Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 208000009889 Herpes Simplex Diseases 0.000 description 1
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 241000701076 Macacine alphaherpesvirus 1 Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 101150003725 TK gene Proteins 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000004523 agglutinating effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000002260 anti-inflammatory agent Substances 0.000 description 1
- 229940124599 anti-inflammatory drug Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000007248 cellular mechanism Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 229960002520 hepatitis vaccine Drugs 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 210000004779 membrane envelope Anatomy 0.000 description 1
- GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 230000000865 phosphorylative effect Effects 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012465 retentate Substances 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Public Health (AREA)
- Oncology (AREA)
- Microbiology (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8009041A FR2480780B1 (fr) | 1980-04-22 | 1980-04-22 | Procede de transformation de cellules, notamment eucaryotes, par un adn circulaire tel que celui du virus de l'hepatite b et preparations contenant les produits d'expression desdits adn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5739784A JPS5739784A (en) | 1982-03-05 |
| JPH0314840B2 true JPH0314840B2 (ko) | 1991-02-27 |
Family
ID=9241228
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56061110A Granted JPS5739784A (en) | 1980-04-22 | 1981-04-22 | Conversion of cell culture |
| JP61197430A Pending JPS6255095A (ja) | 1980-04-22 | 1986-08-25 | ＨＢｓ抗原の形態学的、免疫学的および免疫原的性質を有する抗原の製造方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61197430A Pending JPS6255095A (ja) | 1980-04-22 | 1986-08-25 | ＨＢｓ抗原の形態学的、免疫学的および免疫原的性質を有する抗原の製造方法 |
Country Status (2)
| Country | Link |
|---|---|
| JP (2) | JPS5739784A (ko) |
| FR (1) | FR2480780B1 (ko) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GR76274B (ko) * | 1981-08-04 | 1984-08-04 | Univ California | |
| ZW18282A1 (en) * | 1981-08-31 | 1983-03-23 | Genentech Inc | Preparation of polypeptides in vertebrate cell culture |
| JPS5980615A (ja) * | 1982-10-29 | 1984-05-10 | Takeda Chem Ind Ltd | Ｄｎａおよびその用途 |
| NZ209308A (en) * | 1983-08-30 | 1991-08-27 | Genentech Inc | Vaccine against hsv involving a truncated membrane-free derivative of a membrane-bound protein |
| FR2565995B1 (fr) * | 1984-03-07 | 1987-10-23 | Centre Nat Rech Scient | Cellules hybrides productrices d'un polypeptide determine et obtenu a partir de cellules primaires naturellement capables d'exprimer ce polypeptide ou un polypeptide equivalent, procede pour l'obtention de ces cellules hybrides et application de celles-ci a la production dudit polypeptide |
| FR2560890B1 (fr) * | 1984-03-07 | 1987-10-16 | Grp Genie Genetique | Composition utile pour la fabrication de vaccins contenant des particules portant l'antigene de surface du virus de l'hepatite b et le recepteur de l'albumine serique humaine polymerisee, cellules animales capables de produire de telles particules et procede pour leur obtention |
| EP0171908A3 (en) * | 1984-07-11 | 1987-07-15 | Takeda Chemical Industries, Ltd. | Hepatitis b virus surface antigen and production thereof |
| FR2581393B2 (fr) * | 1985-05-02 | 1989-07-21 | Grp Genie Genetique | Cellules hybrides productrices d'un antigene caracteristique du virus de l'hepatite b obtenu a partir d'hepatocytes et de cellules etablies de singe, procede pour l'obtention de ces cellules hybrides et application de celles-ci a la production de l'antigene susdit |
| JP2810364B2 (ja) * | 1985-10-04 | 1998-10-15 | ジ・アップジョン・カンパニー | 偽狂犬病ウイルス蛋白質 |
| EP0228036A3 (en) * | 1985-12-18 | 1988-09-07 | Microgenesys, Inc. | Method for producing selected polypeptides in virally infected insect cells and polypeptides isolated therefrom |
| US4752565A (en) * | 1986-04-07 | 1988-06-21 | The United States Of America As Represented By The Department Of Health And Human Services | Cell line producing AIDS viral antigens without producing infectious virus particles |
| JPS63230639A (ja) * | 1987-03-19 | 1988-09-27 | Nippon Chem Res Kk | 新規なｂ型肝炎ウイルス表面抗原粒子とその製法 |
| JPH0641584Y2 (ja) * | 1988-02-24 | 1994-11-02 | 株式会社平和 | 遊技球の振分機構 |
| JP2697753B2 (ja) * | 1993-05-24 | 1998-01-14 | 昭 田中 | 直流グロー放電による金属被膜の堆積法 |
| JP4713809B2 (ja) * | 2000-04-07 | 2011-06-29 | ユニバーシティ オブ リードズ イノベイションズ リミテッド | Ｂ型肝炎コア抗原融合タンパク質 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55104887A (en) * | 1978-12-22 | 1980-08-11 | Biogen Nv | Rearranged dna molecule and method |
| JPS5663995A (en) * | 1979-05-24 | 1981-05-30 | Univ California | Nontransit virus |
-
1980
- 1980-04-22 FR FR8009041A patent/FR2480780B1/fr not_active Expired
-
1981
- 1981-04-22 JP JP56061110A patent/JPS5739784A/ja active Granted
-
1986
- 1986-08-25 JP JP61197430A patent/JPS6255095A/ja active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55104887A (en) * | 1978-12-22 | 1980-08-11 | Biogen Nv | Rearranged dna molecule and method |
| JPS5663995A (en) * | 1979-05-24 | 1981-05-30 | Univ California | Nontransit virus |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5739784A (en) | 1982-03-05 |
| FR2480780B1 (fr) | 1985-12-06 |
| FR2480780A1 (fr) | 1981-10-23 |
| JPS6255095A (ja) | 1987-03-10 |
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