JPH0272873A - ヌクレオシドオキシダーゼ及びこれを利用した分析法 - Google Patents
ヌクレオシドオキシダーゼ及びこれを利用した分析法Info
- Publication number
- JPH0272873A JPH0272873A JP63196632A JP19663288A JPH0272873A JP H0272873 A JPH0272873 A JP H0272873A JP 63196632 A JP63196632 A JP 63196632A JP 19663288 A JP19663288 A JP 19663288A JP H0272873 A JPH0272873 A JP H0272873A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- nucleoside
- nucleosides
- reaction
- inosine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 238000000034 method Methods 0.000 title claims description 20
- 239000002777 nucleoside Substances 0.000 claims abstract description 70
- 230000000694 effects Effects 0.000 claims abstract description 50
- 125000003835 nucleoside group Chemical group 0.000 claims abstract description 46
- 150000003833 nucleoside derivatives Chemical class 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000004458 analytical method Methods 0.000 claims abstract description 12
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- 108090000790 Enzymes Proteins 0.000 claims description 110
- 238000006243 chemical reaction Methods 0.000 claims description 44
- 238000012360 testing method Methods 0.000 claims description 31
- 238000007254 oxidation reaction Methods 0.000 claims description 23
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims description 20
- 239000012085 test solution Substances 0.000 claims description 19
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- 239000000203 mixture Substances 0.000 claims description 13
- 238000011161 development Methods 0.000 claims description 12
- 210000002966 serum Anatomy 0.000 claims description 9
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- 108010028584 nucleotidase Proteins 0.000 claims description 7
- 239000002773 nucleotide Substances 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 5
- 125000003729 nucleotide group Chemical group 0.000 claims description 5
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 4
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- 229960003786 inosine Drugs 0.000 abstract description 45
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- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 abstract description 44
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- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract description 6
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- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- AVPCPPOOQICIRJ-UHFFFAOYSA-L sodium glycerol 2-phosphate Chemical compound [Na+].[Na+].OCC(CO)OP([O-])([O-])=O AVPCPPOOQICIRJ-UHFFFAOYSA-L 0.000 description 1
- HYHCSLBZRBJJCH-UHFFFAOYSA-M sodium hydrosulfide Chemical compound [Na+].[SH-] HYHCSLBZRBJJCH-UHFFFAOYSA-M 0.000 description 1
- MWFOPMKUGZLPQA-UHFFFAOYSA-M sodium;3-(n-ethyl-3-methoxyanilino)propane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CCCN(CC)C1=CC=CC(OC)=C1 MWFOPMKUGZLPQA-UHFFFAOYSA-M 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000002211 ultraviolet spectrum Methods 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- UBORTCNDUKBEOP-UUOKFMHZSA-N xanthosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(NC(=O)NC2=O)=C2N=C1 UBORTCNDUKBEOP-UUOKFMHZSA-N 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63196632A JPH0272873A (ja) | 1988-02-04 | 1988-08-05 | ヌクレオシドオキシダーゼ及びこれを利用した分析法 |
| PCT/JP1989/000105 WO1989007137A1 (en) | 1988-02-04 | 1989-02-01 | Nucleoside oxidase and analytical method using same |
| US07/425,217 US5156955A (en) | 1988-02-04 | 1989-02-01 | Nucleoside oxidase and assay method utilizing same |
| EP89902177A EP0353311B1 (en) | 1988-02-04 | 1989-02-01 | Nucleoside oxidase and analytical method using same |
| DE68920659T DE68920659T2 (de) | 1988-02-04 | 1989-02-01 | Nukleosidoxidase und analytisches verfahren. |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2546288 | 1988-02-04 | ||
| JP63-25462 | 1988-02-04 | ||
| JP63196632A JPH0272873A (ja) | 1988-02-04 | 1988-08-05 | ヌクレオシドオキシダーゼ及びこれを利用した分析法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0272873A true JPH0272873A (ja) | 1990-03-13 |
| JPH0544273B2 JPH0544273B2 (en, 2012) | 1993-07-05 |
Family
ID=12166691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63196632A Granted JPH0272873A (ja) | 1988-02-04 | 1988-08-05 | ヌクレオシドオキシダーゼ及びこれを利用した分析法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0272873A (en, 2012) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003156432A (ja) * | 2001-11-22 | 2003-05-30 | Toshiba Corp | 光導波路型バイオケミカルセンサ |
| JP2003207445A (ja) * | 2002-01-16 | 2003-07-25 | Toshiba Corp | 携帯型検査装置および携帯型検査システム |
| JP2003279479A (ja) * | 2002-01-16 | 2003-10-02 | Toshiba Corp | 光導波路型グルコースセンサ及び光導波路型グルコース測定方法 |
| JP2005037403A (ja) * | 2002-01-16 | 2005-02-10 | Toshiba Corp | 光導波路型グルコースセンサ |
-
1988
- 1988-08-05 JP JP63196632A patent/JPH0272873A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003156432A (ja) * | 2001-11-22 | 2003-05-30 | Toshiba Corp | 光導波路型バイオケミカルセンサ |
| JP2003207445A (ja) * | 2002-01-16 | 2003-07-25 | Toshiba Corp | 携帯型検査装置および携帯型検査システム |
| JP2003279479A (ja) * | 2002-01-16 | 2003-10-02 | Toshiba Corp | 光導波路型グルコースセンサ及び光導波路型グルコース測定方法 |
| JP2005037403A (ja) * | 2002-01-16 | 2005-02-10 | Toshiba Corp | 光導波路型グルコースセンサ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0544273B2 (en, 2012) | 1993-07-05 |
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