JPH02258725A - Preventive and treating agent for animal pseudorabies - Google Patents

Abstract

PURPOSE:To obtain a remedy for preventing and treating pseudorabies containing specific crude drugs as active ingredients without causing any problems in formation of strongly toxic viruses and residual vaccine, etc., in animal bodies caused by administration of the vaccine. CONSTITUTION:A preventive and treating agent containing one or two or more crude drugs selected from the group consisting of Caryophylli Flos, Schizonepetae Spica, Artemisiae Folium, Perilla Herba, Rhei Rhizoma, Glycyrrhizae Radix, Moutan Cortex, Paeoniae Radix, Houttuyniae Herba, Artemisiae Capillaris Spco, Loniceras Flos, Cinammoni Cortex, Eucommiae Cortex, Eriobotryae Folium and Forysythiae Fructus as active ingredients. The crude drugs are administered and used in the form of intact crude drug parent chemicals, cut substances thereof, roughly crushed, powdery or granular substances, extract solution obtained by extracting active ingredients of the crude drugs with water or other solvents, diluted or concentrated extracted solutions. The dose thereof is 0.001-10g/kg/day for oral administration and 0.01mg to 1g/kg/day for parenteral administration expressed in terms of the dried crude drug parent chemicals.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an agent for preventing and treating pseudorabies in animals. Specifically, it is a preventive and therapeutic agent for pseudorabies in animals that contains a specific crude drug as an active ingredient.

[Prior Art] Pseudorabies is an acute infectious disease caused by a type of herpes virus.
It is also called Aujeski's disease, after the name of the disease.

This disease affects pigs, cows, horses, goats, sheep, rabbits, dogs,
Cats, mink, foxes, rats, and other animals are affected, and animals other than pigs exhibit severe localized itching symptoms, and as the disease progresses, paralysis of the medulla oblongata, pharynx, jaw, etc., ejaculation, difficulty in breathing, and disordered rhythms occur. etc., resulting in a high rate of death. In the case of pigs, although the symptoms are mild in adult pigs despite their high susceptibility to this disease, symptoms often recover, but in young piglets the symptoms become severe and can be fatal, as in the case of the other animals mentioned above. rate is high.

Conventional methods for preventing and treating pseudorabies include immediately isolating animals infected with pseudorabies and animals that have come into contact with them, and exterminating rats that act as vectors to prevent transmission through breeders, livestock pens, equipment, feed, etc. In order to prevent this, methods such as performing necessary measures such as disinfection have mainly been adopted, but although it is time-consuming, it has not been possible to obtain sufficiently satisfactory results.

In addition, although injections of hyperimmune horse serum or γ-globulin purified from it have some preventive and therapeutic effects, they are still not reliable. Furthermore, attempts have been made to administer weakly virulent vaccines, but although they may be able to prevent the onset of the disease, they cannot prevent infection; on the contrary, they may cause the formation of highly virulent virus carriers,
There were many problems in practical use due to the adverse effects of the vaccine remaining in the animal's body.

[Contents of the Invention] The present inventors have discovered a method that has high preventive and therapeutic effects, requires little effort, and also eliminates problems such as the formation of highly virulent viruses and vaccine residue in the animal body when administered. We have been conducting research on preventive and therapeutic agents for pseudorabies. As a result, the present invention was completed based on the discovery that a specific one among the many herbal medicines is extremely effective in preventing and treating pseudorabies.

Therefore, the present invention includes clove, peony, Wen Shu, Su Ye, Dasho, Licorice, Peony Peel, Zhu medicine, Ten medicines, Fungus Chen Gao, Gold and Silver Flower, Cinnamon Peel,
This is a prophylactic and therapeutic agent for pseudorabies in animals, characterized by containing as an active ingredient one or more crude drugs selected from the group consisting of Morifolia, Loquat, and Liancao.

Clove, peony, mixed leaves, soba, grand prize, licorice, peony skin, ten thousand medicines,
The ten medicines, Bacteria Chengao, Jinyinhua, Cinnamon bark, Du Zhong, Loquat leaf, and Lianyu are all conventionally known as herbal medicines for humans, and these herbal medicines are useful for the prevention and treatment of pseudorabies in animals. It has not been known to be effective, and the present invention is therefore based on a completely unexpected discovery based on the known efficacy of such herbal medicines.

Below, the above-mentioned crude drugs used in the present invention will be briefly explained.

Clove (Caryophylli Fl)
os): The bud of a clove tree just before flowering. It contains eugenol, acetyl eugenol, etc. as ingredients, and is used as a stomachic, anthelmintic, spice, cosmetics, etc.

Schizonepetae 5
pica): It is the flower spike of the sagebrush, and contains menthone, limonene, etc. as ingredients, and is used as an analgesic and anti-inflammatory drug for humans.

Collection of Literature (Artemlsiae Foli)
um): Leaves of Mugwort or Yamabun. Contains eucalybutol sesquiterpene and other ingredients, and is used as a hemostasis, tonic, and blood supplement for humans.

Perillae Herba
: Perilla leaves, with anthocyanin pigment as a component. Contains limonene, etc., and is used as a human sedative, antitussive, etc., and as an antibacterial agent.

Grand Prize (Rhei Rhizoma):
The outer layer of the rhizome of Grand Prize and related plants is peeled as is or vertically divided; it contains the oxyanthraquinone derivatives chrysophane acid, emodin, imodine monoethyl ether, etc., and has a bitter taste that is good for the human stomach. It is used as a medicine, laxative, anti-inflammatory, etc.

Licorice (Glycyrrhizae Ra)
dix): Licorice root, containing glycyrrhizin and other ingredients, and is used as a flavoring agent, antitussive expectorant, etc. for humans.

Moutan Cortex
: Root bark of peony, contains ingredients such as paeonol and benzoic acid, and is used as an analgesic and antispasmodic for humans.

Paeoniaa Radix
): The root of the drug, which contains benzoic acid and other ingredients, and is used as an analgesic for humans.

Houttuynias He
rba): This is the whole family of Heutyami, which contains fercitrin, methyl nonyl ketone, capric acid, etc. as ingredients, and is used as an inflammatory drug for human wounds and urethritis.

mri*c chinko) (Ar't, amisiae
Capillaris Spco): Capillaris Spco is an ear of mugwort, contains kabilarin, kabilarisin, etc. as ingredients, and is used as an anti-inflammatory, antipyretic, and diuretic for humans.

Lonicerae Flo
s): Honeysuckle bud, contains luteolin and other ingredients, and is used as a blood purifier and antidote for humans.

Cinnamon (C4nnamoni Cortex)
): It is the bark of the laurel tree, and contains cinnamyl acetate, cinnamyl acetate, etc., and is used as an antipyretic, analgesic, stomachic, and intestinal medicine for humans.

Eucommiae Corte
x): It is the bark of the Duchu gum tree, and contains gutta percha as an ingredient, and is used as a tonic for humans.

Loquat $ (Eriobotryae Fo)
lium): Loquat leaves, containing ulsone, amygdalin, etc. as ingredients, and is used as a stomachic for humans.

Rapid fire (Forsythiae Fr)
uctus): This fruit is a type of deciduous shrub in the Oleaceae family, and contains oleanol as a component, and is used as an antidote, purulent, anti-inflammatory, diuretic, etc. for humans.

The prophylactic and therapeutic agent of the present invention can be used as a prophylactic and therapeutic agent for pseudorabies in animals such as pigs, cows, horses, goats, sheep, rabbits, dogs, cats, mink, foxes, and dogs. In particular, when used on pigs, cows, horses, chickens, dogs, cats, etc., it brings about great effects in the livestock industry, pig farming industry, and pet industry.

Use the herbal medicine of the present invention to prevent pseudorabies! - In some cases, such herbal medicines can be administered to prevent animals from being infected with pseudorabies, and even if infected, it often does not lead to death. Furthermore, when the herbal medicine of the present invention is used to treat animals infected with pseudorabies, the survival rate is improved.

Among the above-mentioned herbal medicines of the present invention, cloves, daisies, bunshu, soba, and rhubarb are highly effective as preventive and therapeutic agents for pseudorabies, and cloves are particularly excellent.

When administering the above-mentioned crude drug of the present invention to animals, the active ingredient of the crude drug is added to water or other solvent in the form of cut, coarsely ground, or powdered crude drug as it is. It can be used in the form of an extract, or in a diluted or concentrated form. The concentrated form includes a liquid or semi-solid form containing the active ingredient in a concentrated form B (extract) by further subjecting the active ingredient-containing extract to concentration or separation means such as concentration, drying, extraction, and precipitation; There are solid products (lumps, powders, plastic preparations, etc.), and any of them can be used.

When extracting the active ingredients in the herbal medicines with water or other solvents, the raw materials of the herbal medicines, their coarsely ground products, or powders are placed in water, other solvents, or a mixture of the two, immersed,
It is preferable to extract the active ingredient into a liquid by decoction or the like. The liquid temperature and extraction processing time at that time naturally depend on various requirements such as the type of herbal medicine, the type and amount of extraction liquid, ease of extraction of ingredients, thermal stability, and the presence or absence of volatility, but in general is dried; approximately 1 to 1 liter of extraction liquid per 1 g of crude drug substance.
Using ooomQ, at a temperature of about 4 to 100℃, about 30
It is best to do this for 10 minutes to 10 days.

The extraction process may be performed in any state, standing still, stirring, or shaking.

Examples of other solvents other than water that can be used to extract the active ingredient include methanol, ethanol, butanol, acetone, ethyl acetate, ether, methylene chloride, chloroform, benzene, carbon tetrachloride, petroleum ether, etc. .

Among these, water, methanol, ethanol, or a mixture thereof is preferred as the extraction liquid, and water is particularly preferred.

In addition, as a method for further concentrating and recovering the active ingredients as an extract from the active ingredient-containing extract prepared as above, there is a method of partially or completely removing the solvent of the extract with or without heating. , A method of separating the active ingredients by precipitation using a precipitating agent, etc. Adding another solvent to the active ingredient-containing extract, transferring the active ingredients into the other solvent, concentrating it, and removing the other solvent as necessary. A method such as that can be adopted.

Depending on the type of active ingredient contained in the herbal medicine and the type of extraction solvent used to extract the active ingredient, it is necessary to select another solvent for concentration, but usually lower fats are used. group ethers, lower halogen alkanes (chloroform,
Dichloroethane, dibromoethane, etc.), aromatic hydrocarbons (benzene, toluene, xylene, etc.), and other petroleum solvents (petroleum ether, petroleum benzene, ligroin, etc.) can be used.

If the solvent used for extracting and concentrating the active ingredient is prohibited for veterinary medicine,
It is necessary to sufficiently purify the prophylactic and therapeutic agent of the present invention so that it does not ultimately contain the solvent.

In administering the prophylactic and therapeutic agent of the present invention to animals, both oral and parenteral administration can be employed. In the case of oral administration, the active ingredients of the herbal medicines, coarsely ground products or powders of the active ingredients, active ingredient-containing extracts, concentrated extracts, etc. of the above-mentioned herbal medicines can be fed directly to animals as they are, or together with feed or drinking water. Do by doing. In the case of parenteral administration, any of intramuscular administration by injection or coating, intraperitoneal administration, subcutaneous administration, nasal administration, intravenous administration, etc. can be adopted. For parenteral administration, it is advantageous to use the active ingredient in the form of an extract or its concentrate.

The dosage depends on the type of crude drug, the form in which the crude drug is administered (for example, whether it is used as a crude drug or an extract of the active ingredient), the type of animal, and the mode of administration (oral or parenteral administration). ), and if the patient has developed the disease, it depends on the symptoms, etc.
In the case of oral administration, it is usually 0.001-1 og/kg of body weight in terms of dried herbal drug substance or its pulverized product or powder.
It is usually administered in the range of 0.01ff1g to 1g per day over several days to several tens of days, and in the case of parenteral administration, it is usually 0.01ff1g to 1g in terms of dried herbal medicine or its crushed product or powder.
It is preferable to administer the drug at a dose of 100 kg/body x kg/F3 over several days to several tens of days.

Furthermore, the preventive and therapeutic agent of the present invention can be used not only for administration to animals but also as a disinfectant, and in that case, it is preferable to dilute the extract of the herbal medicine and spray it on livestock barns, equipment, and other necessary locations. .

At that time, the diluent should be 0.0 in terms of crude drug substance.
It is preferable to use one having a concentration of 0001 to 1% W/V.

The present invention will be specifically explained below by giving examples, but the present invention is not limited by these examples.

Example 1 Add 05 mQ of liquid containing pseudorabies virus (2x 103 PFU/m1) to 0-05 m12 of Dulbecco's modified MEM medium.
Add 2 liquids
The specimens were sensitized at a temperature of .degree. C. for 1 hour, and the number of black pseudorabies viruses was counted at that time (Example 1.-1: Control example).

Separately, dried clove powder was placed in distilled water at a ratio of 5% V/V and slowly heated at 100° C. for 30 minutes to prepare an extract. This extract was sterilized by filtration using a 0.45 μm filter, and 10-fold serial dilutions were prepared.

0-05m+2 of the same pseudorabies virus-containing solution used in Control Example 1 was added to each 0-05rnn of this 10-fold serial dilution solution, and sensitized at 37°C for 1 hour. Determine the diluent with the maximum dilution ratio in which the black number of f- is 50% or less of that of Control Example 1.
(Example 1-2=Example).

In the above example 1, powders of Jinghuan, Wenzhu, Su Ye, Huizan, Licorice, Peony bark, Paeonia, Juyaku, Bacterium Chengao, Jinyinhua, Cinnamon bark, Duzhong, Duzhong, Loquat and Lianhui were used in place of the end of the clove. -2, the black number of pseudorabies virus was 50 in control example 1 above.
% or less was determined (Example 1-3
~1-xa: Example).

The above results are shown in Table 1 below.

[Table 1] Clove 10' Juyaku 10" Jingkuta 10"
Bacteria perilla 10' cross leaf io3 gold and silver flower 10" So leaf 101 cinnamon 10
8 Rhubarb io” Moritake 10’ Licorice 10” Loquat leaf
102 Botanpi 10” series a
io'Xun Yao 10! From the results in Table 1 above, we can see that clove, peony, wenzhu, soba, rhubarb, licorice, peony bark, takuyaku, ten medicines, fungi human, gold and silver flower, cinnamon,
In the case of any of the extracts of Mori zhong, Loquat leaf and Lianku, even when their extracts were diluted 100 times or more, the growth of pseudorabies virus was reduced by 50% compared to Control Example 1-1 which did not contain the herbal medicine. % or less. Furthermore,
From the results in Table 1, it can be seen that clove is the most effective, followed by Jinghu, Wenzhu, Suyou, and Rhubarb.

Example 2 Dry clove powder was added to distilled water to give a concentration of 5% v/v and slowly heated at 100°C for 30 minutes to prepare an extract. The supernatant of this extract was diluted 5 times with water,
Add this solution only to a water bottle and feed 4 mice (BALB
After feeding the animals freely for 3 days, the pseudorabies virus was administered intraperitoneally at a rate of io''PFU/animal to infect them, and then the above 5-fold diluted clove extract was administered to the animals for 5 days. Five days after pseudorabies virus administration, two animals survived (Example 2-1: Example).

After sterilizing the above 5-fold diluted clove extract, 4 mice (B
0.2an/mouse/intraperitoneally of ALB/C (early 6 weeks old)
It was administered twice at the same time. Pseudorabies virus was intraperitoneally administered to these animals at a rate of IO"PFtJ/animal to infect them. As a result, the number of surviving animals after 5 days of pseudorabies virus administration and infection was 3 (Example 2-2: Example ).

For comparison, four mice (BALB/C pups, 6 weeks old) were intraperitoneally administered with physiological saline twice at a rate of 0 and 2 an/mouse/time, and then pseudorabies virus was administered for 10'.
When the pseudorabies virus was administered intraperitoneally to the animals at a rate of PFU/animal to infect them, the number of surviving animals 5 days after the administration of pseudorabies virus was 0 (Example 2-3: Comparative Example).

From the above results, it is clear that the agent of the present invention containing clove extract
It is found to be effective in preventing and treating pseudorabies in animals.

Example 3 Dried Suji Powder and Dog Yellow Powder were added to distilled water at a concentration of 5% w/v and slowly heated at a temperature of 100°C for 30 minutes to prepare an extract. The supernatants of these extracts were each diluted 5 times with water, and these liquids alone were placed in a water bottle and allowed to be freely ingested by three mice (BALB/C offspring, 6 weeks old) for 7 days. Rabies virus 10'P
The animals were orally administered at a rate of FU/animal to infect them, and then allowed to freely ingest each of the above 5-fold diluted extracts for 5 days. After 5 days of pseudorabies virus administration, the number of survivors was 1 for each.
(Example 3-1: Example).

For comparison, three mice were placed in a water bottle containing only water.
After raising it for several days, it was infected with pseudorabies virus by io'P.
When infected by orally administering the pseudorabies virus at a ratio of FU/animal, the number of surviving animals 5 days after the administration of pseudorabies virus was 0 (Example 3-2: Comparative Example).

The above results demonstrate that the agent of the present invention containing Soo leaf and Rhubarb extract is effective in preventing and treating pseudorabies in animals.

Example 4 Put dried clove ends in water at 10% w/v,
An extract was prepared by heating and boiling for 60 minutes.

The supernatant liquid of this extract was added to the feed of pregnant pigs at 100 mff/7 pigs/day and administered from one week before farrowing until weaning. As a result, pseudorabies was not found in the suckling pigs born from this pregnant pig to which the clove extract was administered, despite the fact that pseudorabies had caused damage to suckling pigs at nearby pig farms. Ta.

Example 5 Pig feed containing 0.5% clove powder (a withdrawal)
20M was given ad libitum over 60 days.

As a result, no outbreak of pseudorabies was observed in these pigs treated with clove powder, even though pseudorabies had occurred at a nearby pig farm.

Example 6 The filtered clove extract prepared in Example 2-1 was mixed with water for 1 hour.
It was diluted 1:0,000 and sprayed on pig pens.

As a result, although pseudorabies had occurred at a nearby pig farm, no outbreak of pseudorabies was observed in this pigpen where the diluted clove extract was sprayed.

[Effects of the Invention] In the present invention, from the group consisting of clove, peony, Bunshu, Suyou, Dasho, licorice, peony bark, Xun medicine, ten medicines, fungi human, gold and silver flower, cinnamon, Du Zhong, loquat leaf, and rapid fire. The prevention and treatment of animal pseudorabies, which is characterized by containing one or more selected crude drugs as active ingredients, does not require much effort, and can also be used to treat pseudorabies that occurs when a vaccine is administered. Pseudorabies can be extremely effectively prevented and treated without causing problems such as the formation of a highly virulent virus or residual vaccine in the animal's body.

Claims (1)

[Claims] Clove, Peony leaves, Soba leaves, Rhubarb, Licorice, Peony skin, Peony leaves,
Prevention of pseudorabies in animals, characterized by containing as an active ingredient one or more of the herbal medicines selected from the group consisting of 10 medicines, 茵蒿, 金和花, cinnamon, Du Zhong, loquat leaves, and Lianxiang. therapeutic agent.