JPH0218069B2 - - Google Patents
Info
- Publication number
- JPH0218069B2 JPH0218069B2 JP56215724A JP21572481A JPH0218069B2 JP H0218069 B2 JPH0218069 B2 JP H0218069B2 JP 56215724 A JP56215724 A JP 56215724A JP 21572481 A JP21572481 A JP 21572481A JP H0218069 B2 JPH0218069 B2 JP H0218069B2
- Authority
- JP
- Japan
- Prior art keywords
- serratiopeptidase
- freeze
- solution
- lactose
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010038132 serratiopeptidase Proteins 0.000 claims description 25
- 229940000634 serratiopeptidase Drugs 0.000 claims description 24
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 10
- 239000008101 lactose Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 6
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- 239000007864 aqueous solution Substances 0.000 description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000002050 international nonproprietary name Substances 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000607714 Serratia sp. Species 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- POOYFSLWYLDQMD-UHFFFAOYSA-N heptacalcium;zinc Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Zn+2] POOYFSLWYLDQMD-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229950000112 serrapeptase Drugs 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000007916 tablet composition Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/98—Preparation of granular or free-flowing enzyme compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicinal Preparation (AREA)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56215724A JPS58134991A (ja) | 1981-12-28 | 1981-12-28 | セラチオペプチタ−ゼの安定化法 |
FR828221599A FR2519021B1 (fr) | 1981-12-28 | 1982-12-22 | Procede de stabilisation de la serrapeptase, composition contenant cette serrapeptase stabilisee et preparation de cette composition |
GR70166A GR78417B (da) | 1981-12-28 | 1982-12-23 | |
IT68523/82A IT1157994B (it) | 1981-12-28 | 1982-12-27 | Procedimento di stabilizzazione della serrapeptasi e composizione contenente serrapeptasi stabilizzata |
ES518579A ES8405841A1 (es) | 1981-12-28 | 1982-12-27 | Un metodo de producir una composicion pulverulenta, que contiene una serrapeptasa estabilizada. |
PT76042A PT76042B (en) | 1981-12-28 | 1982-12-28 | A method of stabilising serrapeptidase |
KR8205845A KR870000237B1 (ko) | 1981-12-28 | 1982-12-28 | 세라펩타제를 안정화 시키는 방법 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56215724A JPS58134991A (ja) | 1981-12-28 | 1981-12-28 | セラチオペプチタ−ゼの安定化法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS58134991A JPS58134991A (ja) | 1983-08-11 |
JPH0218069B2 true JPH0218069B2 (da) | 1990-04-24 |
Family
ID=16677126
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56215724A Granted JPS58134991A (ja) | 1981-12-28 | 1981-12-28 | セラチオペプチタ−ゼの安定化法 |
Country Status (7)
Country | Link |
---|---|
JP (1) | JPS58134991A (da) |
KR (1) | KR870000237B1 (da) |
ES (1) | ES8405841A1 (da) |
FR (1) | FR2519021B1 (da) |
GR (1) | GR78417B (da) |
IT (1) | IT1157994B (da) |
PT (1) | PT76042B (da) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1230300A (en) * | 1983-06-06 | 1987-12-15 | Michael K. Hoskins | Stabilized isoenzyme control product |
US5405767A (en) * | 1992-04-08 | 1995-04-11 | Solvay Enzymes, Inc. | Purified enzyme concentrate and method of preparation |
US6288030B1 (en) * | 1993-12-22 | 2001-09-11 | Amgen Inc. | Stem cell factor formulations and methods |
US5792636A (en) * | 1994-07-20 | 1998-08-11 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing a stable carboxylesterase in dry form |
CU23475A1 (es) * | 2004-07-08 | 2009-12-17 | Ct Ingenieria Genetica Biotech | Composición farmacéutica conteniendo fragmentos polipeptídicos de serralisinas |
KR100667852B1 (ko) | 2006-01-13 | 2007-01-11 | 삼성전자주식회사 | 휴대용 레코더 기기의 잡음 제거 장치 및 그 방법 |
WO2017111569A1 (es) * | 2015-12-21 | 2017-06-29 | Clinica De Avances Oculares, S.A.P.I. De C.V. | Combinación y formulación farmacéutica, para el tratamiento de enfermedades oculares |
CN108309941B (zh) * | 2018-03-20 | 2023-08-11 | 南京工业大学 | 一种舍雷肽酶外用粉剂及其制备方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5534001A (en) * | 1978-08-28 | 1980-03-10 | Noda Sangyo Kagaku Kenkyusho | Stabilization of sarcosine oxidase |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR1352103A (fr) * | 1960-02-26 | 1964-02-14 | Procédé de stabilisation d'enzymes sensibles ou instables spécialement de la catalase | |
FR1533993A (fr) * | 1967-06-13 | 1968-07-26 | Takeda Chemical Industries Ltd | Procédé de préparation d'une protéase |
FR2444465A1 (fr) * | 1978-12-22 | 1980-07-18 | Green Cross Corp | Procede de production d'une preparation thrombolytique |
-
1981
- 1981-12-28 JP JP56215724A patent/JPS58134991A/ja active Granted
-
1982
- 1982-12-22 FR FR828221599A patent/FR2519021B1/fr not_active Expired
- 1982-12-23 GR GR70166A patent/GR78417B/el unknown
- 1982-12-27 IT IT68523/82A patent/IT1157994B/it active
- 1982-12-27 ES ES518579A patent/ES8405841A1/es not_active Expired
- 1982-12-28 KR KR8205845A patent/KR870000237B1/ko active Pre-grant Review Request
- 1982-12-28 PT PT76042A patent/PT76042B/pt unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5534001A (en) * | 1978-08-28 | 1980-03-10 | Noda Sangyo Kagaku Kenkyusho | Stabilization of sarcosine oxidase |
Also Published As
Publication number | Publication date |
---|---|
ES518579A0 (es) | 1984-06-16 |
KR840002901A (ko) | 1984-07-21 |
IT8268523A0 (it) | 1982-12-27 |
FR2519021A1 (fr) | 1983-07-01 |
PT76042B (en) | 1986-01-27 |
KR870000237B1 (ko) | 1987-02-18 |
IT1157994B (it) | 1987-02-18 |
JPS58134991A (ja) | 1983-08-11 |
GR78417B (da) | 1984-09-27 |
ES8405841A1 (es) | 1984-06-16 |
FR2519021B1 (fr) | 1985-07-26 |
PT76042A (en) | 1983-01-01 |
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