JPH02174691A - モノクローナル抗体 - Google Patents
モノクローナル抗体Info
- Publication number
- JPH02174691A JPH02174691A JP1243599A JP24359989A JPH02174691A JP H02174691 A JPH02174691 A JP H02174691A JP 1243599 A JP1243599 A JP 1243599A JP 24359989 A JP24359989 A JP 24359989A JP H02174691 A JPH02174691 A JP H02174691A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- cell
- cells
- cancer
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims abstract description 63
- 102000036639 antigens Human genes 0.000 claims abstract description 26
- 108091007433 antigens Proteins 0.000 claims abstract description 26
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims abstract description 15
- 201000005202 lung cancer Diseases 0.000 claims abstract description 15
- 208000020816 lung neoplasm Diseases 0.000 claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 210000002919 epithelial cell Anatomy 0.000 claims abstract description 7
- 206010028980 Neoplasm Diseases 0.000 claims description 22
- 201000011510 cancer Diseases 0.000 claims description 12
- 210000004072 lung Anatomy 0.000 claims description 8
- 206010029260 Neuroblastoma Diseases 0.000 claims description 7
- 208000005718 Stomach Neoplasms Diseases 0.000 claims description 5
- 210000003238 esophagus Anatomy 0.000 claims description 5
- 206010017758 gastric cancer Diseases 0.000 claims description 5
- 210000002429 large intestine Anatomy 0.000 claims description 5
- 210000000813 small intestine Anatomy 0.000 claims description 5
- 210000002784 stomach Anatomy 0.000 claims description 5
- 201000011549 stomach cancer Diseases 0.000 claims description 5
- 210000001685 thyroid gland Anatomy 0.000 claims description 5
- 206010006187 Breast cancer Diseases 0.000 claims description 4
- 208000026310 Breast neoplasm Diseases 0.000 claims description 4
- 206010009944 Colon cancer Diseases 0.000 claims description 4
- 206010025323 Lymphomas Diseases 0.000 claims description 4
- 206010039491 Sarcoma Diseases 0.000 claims description 4
- 210000000981 epithelium Anatomy 0.000 claims description 4
- 208000032612 Glial tumor Diseases 0.000 claims description 3
- 206010018338 Glioma Diseases 0.000 claims description 3
- 208000001333 Colorectal Neoplasms Diseases 0.000 claims description 2
- 210000004408 hybridoma Anatomy 0.000 abstract description 23
- 239000000427 antigen Substances 0.000 abstract description 19
- 241001465754 Metazoa Species 0.000 abstract description 6
- 206010035226 Plasma cell myeloma Diseases 0.000 abstract description 6
- 201000000050 myeloid neoplasm Diseases 0.000 abstract description 6
- 238000003745 diagnosis Methods 0.000 abstract description 5
- 210000005036 nerve Anatomy 0.000 abstract description 3
- 210000004881 tumor cell Anatomy 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
- 230000009257 reactivity Effects 0.000 description 21
- 238000000034 method Methods 0.000 description 20
- 210000001519 tissue Anatomy 0.000 description 19
- 241000699670 Mus sp. Species 0.000 description 13
- 241000699666 Mus <mouse, genus> Species 0.000 description 10
- 210000003743 erythrocyte Anatomy 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 208000000587 small cell lung carcinoma Diseases 0.000 description 9
- 206010041067 Small cell lung cancer Diseases 0.000 description 6
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 6
- 230000003053 immunization Effects 0.000 description 6
- 238000002649 immunization Methods 0.000 description 6
- 201000009030 Carcinoma Diseases 0.000 description 5
- 108060003951 Immunoglobulin Proteins 0.000 description 5
- 102000018358 immunoglobulin Human genes 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 241001494479 Pecora Species 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- XOJVVFBFDXDTEG-UHFFFAOYSA-N pristane Chemical compound CC(C)CCCC(C)CCCC(C)CCCC(C)C XOJVVFBFDXDTEG-UHFFFAOYSA-N 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 206010003445 Ascites Diseases 0.000 description 3
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 3
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 239000012228 culture supernatant Substances 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000926 neurological effect Effects 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229940104230 thymidine Drugs 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 2
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 2
- 238000011725 BALB/c mouse Methods 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N Hypoxanthine Natural products O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- SXEHKFHPFVVDIR-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine Chemical compound C1=CC(NN)=CC=C1C1=CC=C(NN)C=C1 SXEHKFHPFVVDIR-UHFFFAOYSA-N 0.000 description 2
- 229960003896 aminopterin Drugs 0.000 description 2
- 210000000628 antibody-producing cell Anatomy 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 208000029742 colonic neoplasm Diseases 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000003113 dilution method Methods 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 238000001114 immunoprecipitation Methods 0.000 description 2
- 201000005249 lung adenocarcinoma Diseases 0.000 description 2
- 210000005265 lung cell Anatomy 0.000 description 2
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 2
- 210000004412 neuroendocrine cell Anatomy 0.000 description 2
- 238000011580 nude mouse model Methods 0.000 description 2
- 201000002528 pancreatic cancer Diseases 0.000 description 2
- 208000008443 pancreatic carcinoma Diseases 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- YCOZIPAWZNQLMR-UHFFFAOYSA-N pentadecane Chemical compound CCCCCCCCCCCCCCC YCOZIPAWZNQLMR-UHFFFAOYSA-N 0.000 description 2
- 210000003200 peritoneal cavity Anatomy 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 101710113246 Pectinesterase 3 Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 208000034699 Vitreous floaters Diseases 0.000 description 1
- 210000003567 ascitic fluid Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000000621 bronchi Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 210000003677 hemocyte Anatomy 0.000 description 1
- 229940000351 hemocyte Drugs 0.000 description 1
- 230000000521 hyperimmunizing effect Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 201000009546 lung large cell carcinoma Diseases 0.000 description 1
- 201000005243 lung squamous cell carcinoma Diseases 0.000 description 1
- 210000003126 m-cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 208000016359 neuroblastic tumor Diseases 0.000 description 1
- 230000001272 neurogenic effect Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 208000002154 non-small cell lung carcinoma Diseases 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000007500 overflow downdraw method Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000001415 potassium malate Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 206010041823 squamous cell carcinoma Diseases 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 150000003462 sulfoxides Chemical class 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000002992 thymic effect Effects 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1243599A JPH02174691A (ja) | 1989-09-21 | 1989-09-21 | モノクローナル抗体 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1243599A JPH02174691A (ja) | 1989-09-21 | 1989-09-21 | モノクローナル抗体 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61056358A Division JPS62212399A (ja) | 1986-03-14 | 1986-03-14 | モノクロ−ナル抗体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02174691A true JPH02174691A (ja) | 1990-07-06 |
| JPH0320239B2 JPH0320239B2 (enExample) | 1991-03-18 |
Family
ID=17106212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1243599A Granted JPH02174691A (ja) | 1989-09-21 | 1989-09-21 | モノクローナル抗体 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02174691A (enExample) |
-
1989
- 1989-09-21 JP JP1243599A patent/JPH02174691A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0320239B2 (enExample) | 1991-03-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111777681B (zh) | 一种结合紧密连接蛋白-18.2的抗体及其用途 | |
| KR900003923B1 (ko) | 인체 암종양과 관련된 항원에 대한 단일클론성 항체 | |
| US9988431B2 (en) | Dendritic cell marker and uses thereof | |
| JP2020072681A (ja) | Ｍｕｃ１６に対する抗体およびその使用方法 | |
| WO2021213466A1 (zh) | 抗cd73的抗体及其用途 | |
| CN112703206B (zh) | 靶向肿瘤的重组双功能融合蛋白及其应用 | |
| UA125593C2 (uk) | Антитіло в7-н3, його антигензв'язуючий фрагмент та його медичне застосування | |
| JPS5845407B2 (ja) | 悪性腫瘍抗体の製造方法 | |
| EA006310B1 (ru) | Ассоциированные с ганглиозидами рекомбинантные антитела и их применение в диагностике и лечении опухолей | |
| US20140335104A1 (en) | Methods of Detecting Cells with a Disrupted Cell Membrane, Cells Infected with A Pathogen, Dying Cells or Dead Cells | |
| CN113015751A (zh) | 一种融合蛋白及其用途 | |
| CN108997499A (zh) | 一种抗人pd-l1抗体及其应用 | |
| JPH10501411A (ja) | 細胞周期非依存性グリオーマ細胞表面抗原特異的ヒト・モノクロナール抗体 | |
| JPH08506801A (ja) | Ｃｄ４４エキソン６に対応するペプチド、そのペプチドに特異的な抗体、および、腫瘍診断にそれらの抗体を使用する方法 | |
| EP2402439A1 (en) | Immunization method using cancer cell | |
| CN108997500A (zh) | 一种抗人pd-l1抗体及其应用 | |
| JPH02174691A (ja) | モノクローナル抗体 | |
| JP4651495B2 (ja) | Ｉｓｇ１５タンパク質と特異的に反応するモノクローナル抗体及びそれを産生するハイブリドーマ並びに癌およびウイルス感染の検出方法 | |
| CN118307677B (zh) | 一种针对间皮素的单克隆抗体及应用 | |
| JPS6115897A (ja) | ヒト肝癌細胞を特異的に認識するモノクロ−ナル抗体 | |
| JPH0678787A (ja) | ヒトムチン性卵巣腫瘍に対するモノクローナル抗体及び該抗体を用いるヒト卵巣腫瘍の検出法 | |
| JPS62212399A (ja) | モノクロ−ナル抗体 | |
| CN113939530A (zh) | Semg2抗体及其用途 | |
| JP2013013327A (ja) | Ｍａｎｓｃ１蛋白質に結合し、抗癌活性を有する抗体 | |
| JPS60199830A (ja) | モノクロ−ナル抗体 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |