JP7825830B2 - ゲノム改変方法及びゲノム改変キット - Google Patents

ゲノム改変方法及びゲノム改変キット

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Publication number
JP7825830B2
JP7825830B2 JP2022514064A JP2022514064A JP7825830B2 JP 7825830 B2 JP7825830 B2 JP 7825830B2 JP 2022514064 A JP2022514064 A JP 2022514064A JP 2022514064 A JP2022514064 A JP 2022514064A JP 7825830 B2 JP7825830 B2 JP 7825830B2
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sequence
homology arm
alleles
donor dna
selectable marker
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JPWO2021206054A1 (https=
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康則 相澤
知幸 大野
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Logomix Inc Japan
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Logomix Inc Japan
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2022514064A 2020-04-06 2021-04-05 ゲノム改変方法及びゲノム改変キット Active JP7825830B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2020068266 2020-04-06
JP2020068266 2020-04-06
JP2020141335 2020-08-25
JP2020141335 2020-08-25
PCT/JP2021/014495 WO2021206054A1 (ja) 2020-04-06 2021-04-05 ゲノム改変方法及びゲノム改変キット

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JP7825830B2 true JP7825830B2 (ja) 2026-03-09

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US (1) US20230147287A1 (https=)
EP (1) EP4134431A4 (https=)
JP (1) JP7825830B2 (https=)
KR (1) KR20220164748A (https=)
CN (1) CN115552002A (https=)
AU (1) AU2021254373A1 (https=)
CA (1) CA3179410A1 (https=)
IL (1) IL297078A (https=)
TW (1) TW202204611A (https=)
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Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119072544A (zh) 2022-04-01 2024-12-03 株式会社标志融合 适合于基因工程、细胞工程和细胞药物的细胞及其制造方法
JP7212982B1 (ja) 2022-10-05 2023-01-26 株式会社Logomix 細胞ライブラリおよびその製造方法
WO2024219474A1 (ja) * 2023-04-21 2024-10-24 株式会社Logomix Ｋｉｒ遺伝子クラスター領域、ｌｉｌｒ遺伝子クラスター領域およびｋｌｒ遺伝子クラスター領域のいずれか１以上の領域において欠失を有する細胞およびその製造方法
WO2025033445A1 (ja) 2023-08-07 2025-02-13 株式会社Logomix 遺伝子をコンディショナルに不活化させる方法、およびそのための細胞

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JP2016514480A (ja) 2013-04-05 2016-05-23 ダウ アグロサイエンシィズ エルエルシー 植物のゲノム内に外因性配列を組み込むための方法および組成物
WO2019018534A1 (en) 2017-07-18 2019-01-24 The Board Of Trustees Of Leland Stanford Junior University GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY

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US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US10787684B2 (en) * 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
KR102170502B1 (ko) * 2013-12-11 2020-10-28 리제너론 파마슈티칼스 인코포레이티드 게놈의 표적화된 변형을 위한 방법 및 조성물
WO2017191503A1 (en) * 2016-05-05 2017-11-09 Crispr Therapeutics Ag Materials and methods for treatment of hemoglobinopathies
CN106520829B (zh) * 2016-10-17 2019-12-17 扬州大学 一种终止双等位基因转录的方法
WO2019216338A1 (ja) * 2018-05-08 2019-11-14 国立大学法人大阪大学 ホモ接合型細胞の作製方法

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JP2016514480A (ja) 2013-04-05 2016-05-23 ダウ アグロサイエンシィズ エルエルシー 植物のゲノム内に外因性配列を組み込むための方法および組成物
WO2019018534A1 (en) 2017-07-18 2019-01-24 The Board Of Trustees Of Leland Stanford Junior University GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY

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Kazuto YOSHIMI et al.，"ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes"，Nature Communications，2016年01月20日，Vol. 7, No. 1，DOI: 10.1038/ncomms10431
LIU Y et al.，"Biallelic insertion of a transcriptional terminator via the CRISPR/Cas9 system efficiently silences expression of protein-coding and non-coding RNA genes."，The Journal of biological chemistry，2017年02月14日，Vol. 292, No. 14，p.5624-5633
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AU2021254373A1 (en) 2022-11-03
TW202204611A (zh) 2022-02-01
EP4134431A4 (en) 2024-06-05
US20230147287A1 (en) 2023-05-11
CN115552002A (zh) 2022-12-30
EP4134431A1 (en) 2023-02-15
IL297078A (en) 2022-12-01
JPWO2021206054A1 (https=) 2021-10-14
WO2021206054A1 (ja) 2021-10-14
CA3179410A1 (en) 2021-10-14
KR20220164748A (ko) 2022-12-13

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