CN115552002A - 基因组改造方法及基因组改造试剂盒 - Google Patents

基因组改造方法及基因组改造试剂盒 Download PDF

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CN115552002A
CN115552002A CN202180026858.3A CN202180026858A CN115552002A CN 115552002 A CN115552002 A CN 115552002A CN 202180026858 A CN202180026858 A CN 202180026858A CN 115552002 A CN115552002 A CN 115552002A
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sequence
homology arm
alleles
donor dna
genome
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相泽康则
大野知幸
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Tokyo Institute of Technology NUC
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CN202180026858.3A 2020-04-06 2021-04-05 基因组改造方法及基因组改造试剂盒 Pending CN115552002A (zh)

Applications Claiming Priority (5)

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JP2020068266 2020-04-06
JP2020-068266 2020-04-06
JP2020141335 2020-08-25
JP2020-141335 2020-08-25
PCT/JP2021/014495 WO2021206054A1 (ja) 2020-04-06 2021-04-05 ゲノム改変方法及びゲノム改変キット

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EP (1) EP4134431A4 (https=)
JP (1) JP7825830B2 (https=)
KR (1) KR20220164748A (https=)
CN (1) CN115552002A (https=)
AU (1) AU2021254373A1 (https=)
CA (1) CA3179410A1 (https=)
IL (1) IL297078A (https=)
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Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
CN119072544A (zh) 2022-04-01 2024-12-03 株式会社标志融合 适合于基因工程、细胞工程和细胞药物的细胞及其制造方法
JP7212982B1 (ja) 2022-10-05 2023-01-26 株式会社Logomix 細胞ライブラリおよびその製造方法
WO2024219474A1 (ja) * 2023-04-21 2024-10-24 株式会社Logomix Ｋｉｒ遺伝子クラスター領域、ｌｉｌｒ遺伝子クラスター領域およびｋｌｒ遺伝子クラスター領域のいずれか１以上の領域において欠失を有する細胞およびその製造方法
WO2025033445A1 (ja) 2023-08-07 2025-02-13 株式会社Logomix 遺伝子をコンディショナルに不活化させる方法、およびそのための細胞

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CN102094036A (zh) * 2010-12-13 2011-06-15 西北农林科技大学 一种等位基因双敲除打靶载体系统及其构建方法
CN106520829A (zh) * 2016-10-17 2017-03-22 扬州大学 一种终止双等位基因转录的方法
WO2019018534A1 (en) * 2017-07-18 2019-01-24 The Board Of Trustees Of Leland Stanford Junior University GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY

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US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
CN105263312A (zh) * 2013-04-05 2016-01-20 美国陶氏益农公司 用于在植物基因组内整合外源序列的方法和组合物
US10787684B2 (en) * 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
KR102170502B1 (ko) * 2013-12-11 2020-10-28 리제너론 파마슈티칼스 인코포레이티드 게놈의 표적화된 변형을 위한 방법 및 조성물
WO2017191503A1 (en) * 2016-05-05 2017-11-09 Crispr Therapeutics Ag Materials and methods for treatment of hemoglobinopathies
WO2019216338A1 (ja) * 2018-05-08 2019-11-14 国立大学法人大阪大学 ホモ接合型細胞の作製方法

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
CN102094036A (zh) * 2010-12-13 2011-06-15 西北农林科技大学 一种等位基因双敲除打靶载体系统及其构建方法
CN106520829A (zh) * 2016-10-17 2017-03-22 扬州大学 一种终止双等位基因转录的方法
WO2019018534A1 (en) * 2017-07-18 2019-01-24 The Board Of Trustees Of Leland Stanford Junior University GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY

Non-Patent Citations (4)

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Title
KAZUYA IKEDA等: "Efficient scarless genome editing in human pluripotent stem cells", NATURE METHODS, vol. 15, 30 November 2018 (2018-11-30), pages 1045 - 1047 *
SHIN-IL KIM等: "Microhomology-assisted scarless genome editing in human iPSCs", NATURE COMMUNICATIONS, vol. 9, no. 1, 5 March 2018 (2018-03-05), pages 939 *
SUPHARATTANASITTHI, W.等: "CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection.", SCIENTIFIC REPORTS, vol. 9, no. 1, 17 January 2019 (2019-01-17), pages 1 - 7 *
WASU SUPHARATTANASITTHI等: "CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection", SCIENTIFIC REPORTS, vol. 9, no. 1, 17 January 2019 (2019-01-17), pages 1 - 7 *

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AU2021254373A1 (en) 2022-11-03
TW202204611A (zh) 2022-02-01
EP4134431A4 (en) 2024-06-05
US20230147287A1 (en) 2023-05-11
JP7825830B2 (ja) 2026-03-09
EP4134431A1 (en) 2023-02-15
IL297078A (en) 2022-12-01
JPWO2021206054A1 (https=) 2021-10-14
WO2021206054A1 (ja) 2021-10-14
CA3179410A1 (en) 2021-10-14
KR20220164748A (ko) 2022-12-13

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