JP7734415B2 - 核酸配列を欠乏および富化するための方法およびキット - Google Patents

核酸配列を欠乏および富化するための方法およびキット

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Publication number
JP7734415B2
JP7734415B2 JP2021522452A JP2021522452A JP7734415B2 JP 7734415 B2 JP7734415 B2 JP 7734415B2 JP 2021522452 A JP2021522452 A JP 2021522452A JP 2021522452 A JP2021522452 A JP 2021522452A JP 7734415 B2 JP7734415 B2 JP 7734415B2
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Prior art keywords
nucleic acid
sample
acid molecules
sample solution
acid sequence
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JP2021522452A
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Japanese (ja)
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JP2022505788A5 (https=
JP2022505788A (ja
Inventor
ローゼンバーグ，アレクサンダー・ビィ
ロコ，チャールズ
シーリグ，ゲオルク
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ユニヴァーシティ オブ ワシントン
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021522452A 2018-10-24 2019-10-24 核酸配列を欠乏および富化するための方法およびキット Active JP7734415B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025044821A JP2025098115A (ja) 2018-10-24 2025-03-19 核酸配列を欠乏および富化するための方法およびキット

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862750169P 2018-10-24 2018-10-24
US62/750,169 2018-10-24
PCT/US2019/057939 WO2020086896A1 (en) 2018-10-24 2019-10-24 Methods and kits for depletion and enrichment of nucleic acid sequences

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025044821A Division JP2025098115A (ja) 2018-10-24 2025-03-19 核酸配列を欠乏および富化するための方法およびキット

Publications (3)

Publication Number Publication Date
JP2022505788A JP2022505788A (ja) 2022-01-14
JP2022505788A5 JP2022505788A5 (https=) 2022-10-27
JP7734415B2 true JP7734415B2 (ja) 2025-09-05

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ID=70332116

Family Applications (2)

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JP2021522452A Active JP7734415B2 (ja) 2018-10-24 2019-10-24 核酸配列を欠乏および富化するための方法およびキット
JP2025044821A Withdrawn JP2025098115A (ja) 2018-10-24 2025-03-19 核酸配列を欠乏および富化するための方法およびキット

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Country Status (7)

Country Link
US (1) US20210388415A1 (https=)
EP (1) EP3870696B1 (https=)
JP (2) JP7734415B2 (https=)
CN (2) CN113166742B (https=)
AU (1) AU2019368024B2 (https=)
CA (1) CA3113808A1 (https=)
WO (1) WO2020086896A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112022026860A2 (pt) * 2020-09-14 2023-04-11 Illumina Inc Composições e métodos para amplificar polinucleotídeos

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016647A1 (en) 2000-08-24 2002-02-28 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
JP2011509660A (ja) 2008-01-14 2011-03-31 アプライド バイオシステムズ， エルエルシー リボ核酸を検出するための組成物、方法およびキット
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2561381C (en) * 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
CA2621267A1 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
KR20100083584A (ko) * 2009-01-14 2010-07-22 삼성전자주식회사 역전사된 단일가닥 ｄｎａ를 측정하는 방법, 역전사 효소의활성을 측정하는 방법, 및 그를 위한 키트
EP2807292B1 (en) * 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20150284783A1 (en) * 2012-05-21 2015-10-08 Agena Bioscience, Inc. Methods and compositions for analyzing nucleic acid
HK1217518A1 (zh) * 2012-12-03 2017-01-13 以琳生物药物有限公司 核酸制备和分析的组合物和方法
CA2906365A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US10472666B2 (en) * 2016-02-15 2019-11-12 Roche Sequencing Solutions, Inc. System and method for targeted depletion of nucleic acids
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016647A1 (en) 2000-08-24 2002-02-28 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
JP2011509660A (ja) 2008-01-14 2011-03-31 アプライド バイオシステムズ， エルエルシー リボ核酸を検出するための組成物、方法およびキット
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences

Also Published As

Publication number Publication date
WO2020086896A1 (en) 2020-04-30
EP3870696A4 (en) 2022-08-03
CA3113808A1 (en) 2020-04-30
CN113166742A (zh) 2021-07-23
AU2019368024B2 (en) 2026-02-12
EP3870696C0 (en) 2025-03-26
CN119320816A (zh) 2025-01-17
EP3870696B1 (en) 2025-03-26
AU2019368024A1 (en) 2021-04-15
US20210388415A1 (en) 2021-12-16
CN113166742B (zh) 2024-10-01
EP3870696A1 (en) 2021-09-01
JP2022505788A (ja) 2022-01-14
JP2025098115A (ja) 2025-07-01

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