CA3113808A1 - Methods and kits for depletion and enrichment of nucleic acid sequences - Google Patents

Methods and kits for depletion and enrichment of nucleic acid sequences Download PDF

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Publication number
CA3113808A1
CA3113808A1 CA3113808A CA3113808A CA3113808A1 CA 3113808 A1 CA3113808 A1 CA 3113808A1 CA 3113808 A CA3113808 A CA 3113808A CA 3113808 A CA3113808 A CA 3113808A CA 3113808 A1 CA3113808 A1 CA 3113808A1
Authority
CA
Canada
Prior art keywords
nucleic acid
acid molecules
sample
sample solution
acid molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3113808A
Other languages
English (en)
French (fr)
Inventor
Alexander B. Rosenberg
Charles ROCO
Georg Seelig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Original Assignee
University of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington filed Critical University of Washington
Publication of CA3113808A1 publication Critical patent/CA3113808A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3113808A 2018-10-24 2019-10-24 Methods and kits for depletion and enrichment of nucleic acid sequences Pending CA3113808A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862750169P 2018-10-24 2018-10-24
US62/750,169 2018-10-24
PCT/US2019/057939 WO2020086896A1 (en) 2018-10-24 2019-10-24 Methods and kits for depletion and enrichment of nucleic acid sequences

Publications (1)

Publication Number Publication Date
CA3113808A1 true CA3113808A1 (en) 2020-04-30

Family

ID=70332116

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3113808A Pending CA3113808A1 (en) 2018-10-24 2019-10-24 Methods and kits for depletion and enrichment of nucleic acid sequences

Country Status (7)

Country Link
US (1) US20210388415A1 (https=)
EP (1) EP3870696B1 (https=)
JP (2) JP7734415B2 (https=)
CN (2) CN113166742B (https=)
AU (1) AU2019368024B2 (https=)
CA (1) CA3113808A1 (https=)
WO (1) WO2020086896A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112022026860A2 (pt) * 2020-09-14 2023-04-11 Illumina Inc Composições e métodos para amplificar polinucleotídeos

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001285219A1 (en) * 2000-08-24 2002-03-04 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
CA2561381C (en) * 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
CA2621267A1 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
AU2009205523A1 (en) * 2008-01-14 2009-07-23 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid
KR20100083584A (ko) * 2009-01-14 2010-07-22 삼성전자주식회사 역전사된 단일가닥 ｄｎａ를 측정하는 방법, 역전사 효소의활성을 측정하는 방법, 및 그를 위한 키트
EP2807292B1 (en) * 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20150284783A1 (en) * 2012-05-21 2015-10-08 Agena Bioscience, Inc. Methods and compositions for analyzing nucleic acid
CA2877094A1 (en) * 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
HK1217518A1 (zh) * 2012-12-03 2017-01-13 以琳生物药物有限公司 核酸制备和分析的组合物和方法
CA2906365A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US10472666B2 (en) * 2016-02-15 2019-11-12 Roche Sequencing Solutions, Inc. System and method for targeted depletion of nucleic acids
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof

Also Published As

Publication number Publication date
WO2020086896A1 (en) 2020-04-30
EP3870696A4 (en) 2022-08-03
CN113166742A (zh) 2021-07-23
JP7734415B2 (ja) 2025-09-05
AU2019368024B2 (en) 2026-02-12
EP3870696C0 (en) 2025-03-26
CN119320816A (zh) 2025-01-17
EP3870696B1 (en) 2025-03-26
AU2019368024A1 (en) 2021-04-15
US20210388415A1 (en) 2021-12-16
CN113166742B (zh) 2024-10-01
EP3870696A1 (en) 2021-09-01
JP2022505788A (ja) 2022-01-14
JP2025098115A (ja) 2025-07-01

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