CN113166742B - 用于消耗和富集核酸序列的方法和试剂盒 - Google Patents

用于消耗和富集核酸序列的方法和试剂盒 Download PDF

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Publication number
CN113166742B
CN113166742B CN201980070102.1A CN201980070102A CN113166742B CN 113166742 B CN113166742 B CN 113166742B CN 201980070102 A CN201980070102 A CN 201980070102A CN 113166742 B CN113166742 B CN 113166742B
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nucleic acid
acid molecules
sample
sample solution
acid sequence
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Chinese (zh)
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CN113166742A (zh
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A·B·罗森伯格
C·若克
G·希莉格
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University of Washington
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University of Washington
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201980070102.1A 2018-10-24 2019-10-24 用于消耗和富集核酸序列的方法和试剂盒 Active CN113166742B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202411269316.0A CN119320816A (zh) 2018-10-24 2019-10-24 用于消耗和富集核酸序列的方法和试剂盒

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862750169P 2018-10-24 2018-10-24
US62/750169 2018-10-24
PCT/US2019/057939 WO2020086896A1 (en) 2018-10-24 2019-10-24 Methods and kits for depletion and enrichment of nucleic acid sequences

Related Child Applications (1)

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CN202411269316.0A Division CN119320816A (zh) 2018-10-24 2019-10-24 用于消耗和富集核酸序列的方法和试剂盒

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CN113166742A CN113166742A (zh) 2021-07-23
CN113166742B true CN113166742B (zh) 2024-10-01

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US (1) US20210388415A1 (https=)
EP (1) EP3870696B1 (https=)
JP (2) JP7734415B2 (https=)
CN (2) CN113166742B (https=)
AU (1) AU2019368024B2 (https=)
CA (1) CA3113808A1 (https=)
WO (1) WO2020086896A1 (https=)

Families Citing this family (1)

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Publication number Priority date Publication date Assignee Title
BR112022026860A2 (pt) * 2020-09-14 2023-04-11 Illumina Inc Composições e métodos para amplificar polinucleotídeos

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104093890A (zh) * 2012-01-26 2014-10-08 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
CN104619894A (zh) * 2012-06-18 2015-05-13 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001285219A1 (en) * 2000-08-24 2002-03-04 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
CA2561381C (en) * 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
CA2621267A1 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
AU2009205523A1 (en) * 2008-01-14 2009-07-23 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid
KR20100083584A (ko) * 2009-01-14 2010-07-22 삼성전자주식회사 역전사된 단일가닥 ｄｎａ를 측정하는 방법, 역전사 효소의활성을 측정하는 방법, 및 그를 위한 키트
US20150284783A1 (en) * 2012-05-21 2015-10-08 Agena Bioscience, Inc. Methods and compositions for analyzing nucleic acid
HK1217518A1 (zh) * 2012-12-03 2017-01-13 以琳生物药物有限公司 核酸制备和分析的组合物和方法
CA2906365A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US10472666B2 (en) * 2016-02-15 2019-11-12 Roche Sequencing Solutions, Inc. System and method for targeted depletion of nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104093890A (zh) * 2012-01-26 2014-10-08 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
CN104619894A (zh) * 2012-06-18 2015-05-13 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof

Also Published As

Publication number Publication date
WO2020086896A1 (en) 2020-04-30
EP3870696A4 (en) 2022-08-03
CA3113808A1 (en) 2020-04-30
CN113166742A (zh) 2021-07-23
JP7734415B2 (ja) 2025-09-05
AU2019368024B2 (en) 2026-02-12
EP3870696C0 (en) 2025-03-26
CN119320816A (zh) 2025-01-17
EP3870696B1 (en) 2025-03-26
AU2019368024A1 (en) 2021-04-15
US20210388415A1 (en) 2021-12-16
EP3870696A1 (en) 2021-09-01
JP2022505788A (ja) 2022-01-14
JP2025098115A (ja) 2025-07-01

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