JP7691975B2 - 単一細胞分析 - Google Patents

単一細胞分析 Download PDF

Info

Publication number
JP7691975B2
JP7691975B2 JP2022506428A JP2022506428A JP7691975B2 JP 7691975 B2 JP7691975 B2 JP 7691975B2 JP 2022506428 A JP2022506428 A JP 2022506428A JP 2022506428 A JP2022506428 A JP 2022506428A JP 7691975 B2 JP7691975 B2 JP 7691975B2
Authority
JP
Japan
Prior art keywords
cases
cell
cells
sequencing
pta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022506428A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022543051A5 (https=
JP2022543051A (ja
Inventor
ガワッド，チャールズ
エー．エー． ウェスト，ジェイ
Original Assignee
バイオスクリブ ゲノミックス，インク．
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by バイオスクリブ ゲノミックス，インク． filed Critical バイオスクリブ ゲノミックス，インク．
Publication of JP2022543051A publication Critical patent/JP2022543051A/ja
Publication of JP2022543051A5 publication Critical patent/JP2022543051A5/ja
Priority to JP2025090040A priority Critical patent/JP2025143255A/ja
Application granted granted Critical
Publication of JP7691975B2 publication Critical patent/JP7691975B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2022506428A 2019-07-31 2020-07-30 単一細胞分析 Active JP7691975B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025090040A JP2025143255A (ja) 2019-07-31 2025-05-29 単一細胞分析

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962881183P 2019-07-31 2019-07-31
US62/881,183 2019-07-31
PCT/US2020/044338 WO2021022085A2 (en) 2019-07-31 2020-07-30 Single cell analysis

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025090040A Division JP2025143255A (ja) 2019-07-31 2025-05-29 単一細胞分析

Publications (3)

Publication Number Publication Date
JP2022543051A JP2022543051A (ja) 2022-10-07
JP2022543051A5 JP2022543051A5 (https=) 2023-08-08
JP7691975B2 true JP7691975B2 (ja) 2025-06-12

Family

ID=74228691

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022506428A Active JP7691975B2 (ja) 2019-07-31 2020-07-30 単一細胞分析
JP2025090040A Pending JP2025143255A (ja) 2019-07-31 2025-05-29 単一細胞分析

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025090040A Pending JP2025143255A (ja) 2019-07-31 2025-05-29 単一細胞分析

Country Status (10)

Country Link
US (1) US20230220377A1 (https=)
EP (1) EP4004201A4 (https=)
JP (2) JP7691975B2 (https=)
KR (1) KR20220041875A (https=)
CN (1) CN114555802B (https=)
AU (1) AU2020322027A1 (https=)
CA (1) CA3149610A1 (https=)
IL (1) IL290245A (https=)
MX (1) MX2022001324A (https=)
WO (1) WO2021022085A2 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2937927T3 (es) 2018-01-29 2023-04-03 St Jude Childrens Res Hospital Inc Método para la amplificación de ácidos nucleicos
AU2020321370A1 (en) * 2019-07-31 2022-03-03 BioSkryb Genomics, Inc. Genetic mutational analysis
US20240167082A1 (en) * 2021-03-19 2024-05-23 Paris Sciences Et Lettres Methods for the selective analysis of cells or organelles
CN113046416B (zh) * 2021-03-24 2026-01-09 北京百迈客生物科技有限公司 一种纳米孔全长转录组文库构建方法
CN113151425B (zh) * 2021-04-08 2023-01-06 中国计量科学研究院 基于关键指标提高准确性的单细胞测序方法
JP2024516637A (ja) * 2021-04-26 2024-04-16 ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッド ポリヌクレオチド配列の変化を特徴決定するための組成物および方法
EP4388128A4 (en) * 2021-08-16 2025-07-09 Bioskryb Genomics Inc EMBRYONIC NUCLEIC ACID ANALYSIS
US20230113026A1 (en) * 2021-09-29 2023-04-13 Battelle Memorial Institute Apparatuses and Methods for Performing Multiple Omics Analysis and Processing Analyte Mixtures
CN113943729B (zh) * 2021-10-20 2023-05-16 翌圣生物科技(上海)股份有限公司 U型接头及采用u型接头介导的磁珠偶联转座酶进行rna快速均一化建库的方法
WO2023212223A1 (en) * 2022-04-28 2023-11-02 BioSkryb Genomics, Inc. Single cell multiomics
WO2023215524A2 (en) * 2022-05-05 2023-11-09 BioSkryb Genomics, Inc. Primary template-directed amplification and methods thereof
CN115144519A (zh) * 2022-06-30 2022-10-04 上海交通大学 基于无机纳米颗粒的单细胞样品指纹图谱检测方法和应用
WO2024026376A2 (en) * 2022-07-27 2024-02-01 BioSkryb Genomics, Inc. Methods and systems for multiomic analysis
CN115820802A (zh) * 2022-10-31 2023-03-21 北京百迈客生物科技有限公司 一种单细胞全长转录组文库的构建方法及其应用
CN120099141B (zh) * 2025-03-13 2026-03-06 江苏硕世生物科技股份有限公司 一种pcr长扩增子的二代测序文库构建方法及应用

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150368694A1 (en) 2014-06-23 2015-12-24 Yale University Methods for closed chromatin mapping and dna methylation analysis for single cells
JP2016511007A (ja) 2013-03-15 2016-04-14 ニューゲン テクノロジーズ， インコーポレイテッド 鎖になったｒｎａまたはｄｎａのライブラリを生成するための方法、組成物およびキット
US20180216160A1 (en) 2015-02-04 2018-08-02 The Regents Of The University Of California Sequencing of Nucleic Acids via Barcoding in Discrete Entities
JP2018527020A (ja) 2015-07-21 2018-09-20 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸の配列決定方法及びシステム
WO2018174827A1 (en) 2017-03-24 2018-09-27 National University Of Singapore Methods for multiplex detection of molecules
WO2019028047A1 (en) 2017-08-01 2019-02-07 Illumina, Inc SPATIAL INDEXING OF GENETIC MATERIAL AND PREPARATION OF PHARMACOTOQUE USING HYDROGEL BALLS AND FLOW CELLS
WO2019121842A1 (en) 2017-12-21 2019-06-27 F. Hoffmann-La Roche Ag Target enrichment by unidirectional dual probe primer extension
JP2021511794A (ja) 2018-01-29 2021-05-13 セント ジュード チルドレンズ リサーチ ホスピタル，インコーポレイティド 核酸増幅のための方法
JP2022543375A (ja) 2019-07-31 2022-10-12 バイオスクリブ ゲノミックス，インク． 遺伝子変異分析

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
DE69812329T2 (de) 1997-11-18 2004-02-12 Bio-Rad Laboratories, Inc., Hercules Multiplex-zufluss-immunotest mit magnetischen teilchen als festphase
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1204869B1 (en) 1999-08-17 2008-10-22 Luminex Corporation Method for analyzing a number of samples from a variety of sources for a single analyte
WO2001013119A1 (en) 1999-08-17 2001-02-22 Luminex Corporation Encapsulation of fluorescent particles
AU2001280796A1 (en) 2000-07-25 2002-02-05 The Regents Of The University Of California Electrowetting-driven micropumping
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7163612B2 (en) 2001-11-26 2007-01-16 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
JP4773360B2 (ja) 2003-11-17 2011-09-14 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 流体を操作するためのシステム
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
EP1704402B1 (en) 2004-01-14 2016-05-11 Luminex Corporation Methods and systems for dynamic range expansion
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
WO2006079016A1 (en) 2005-01-20 2006-07-27 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
EP4071458B1 (en) 2005-09-21 2025-11-05 Luminex Corporation Methods and systems for image data processing
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
US20070207513A1 (en) 2006-03-03 2007-09-06 Luminex Corporation Methods, Products, and Kits for Identifying an Analyte in a Sample
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
ATE542921T1 (de) 2006-04-18 2012-02-15 Advanced Liquid Logic Inc Pyrosequenzierung auf tröpfchenbasis
JP4881950B2 (ja) 2006-07-10 2012-02-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス
WO2008055256A2 (en) 2006-11-02 2008-05-08 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
ES2423930T3 (es) 2007-02-09 2013-09-25 Advanced Liquid Logic, Inc. Dispositivos actuadores de gotitas y métodos que emplean perlas magnéticas
WO2008116221A1 (en) 2007-03-22 2008-09-25 Advanced Liquid Logic, Inc. Bead sorting on a droplet actuator
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
US8926811B2 (en) 2007-06-27 2015-01-06 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9309511B2 (en) * 2007-08-28 2016-04-12 The Johns Hopkins University Functional assay for identification of loss-of-function mutations in genes
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2012071096A2 (en) 2010-09-03 2012-05-31 The Johns Hopkins University Arid1a and ppp2r1a mutations in cancer
GB2497912B (en) 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
ES2986436T3 (es) 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
CN109791157B (zh) * 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
CA3047328A1 (en) * 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
CN117821565A (zh) * 2017-03-08 2024-04-05 芝加哥大学 高灵敏度dna甲基化分析方法
DK3635136T3 (da) 2017-06-07 2022-01-10 Univ Oregon Health & Science Enkeltcelle-helgenombiblioteker til methyleringssekvensering
WO2019084055A1 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016511007A (ja) 2013-03-15 2016-04-14 ニューゲン テクノロジーズ， インコーポレイテッド 鎖になったｒｎａまたはｄｎａのライブラリを生成するための方法、組成物およびキット
US20150368694A1 (en) 2014-06-23 2015-12-24 Yale University Methods for closed chromatin mapping and dna methylation analysis for single cells
US20180216160A1 (en) 2015-02-04 2018-08-02 The Regents Of The University Of California Sequencing of Nucleic Acids via Barcoding in Discrete Entities
JP2018527020A (ja) 2015-07-21 2018-09-20 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸の配列決定方法及びシステム
WO2018174827A1 (en) 2017-03-24 2018-09-27 National University Of Singapore Methods for multiplex detection of molecules
WO2019028047A1 (en) 2017-08-01 2019-02-07 Illumina, Inc SPATIAL INDEXING OF GENETIC MATERIAL AND PREPARATION OF PHARMACOTOQUE USING HYDROGEL BALLS AND FLOW CELLS
WO2019121842A1 (en) 2017-12-21 2019-06-27 F. Hoffmann-La Roche Ag Target enrichment by unidirectional dual probe primer extension
JP2021511794A (ja) 2018-01-29 2021-05-13 セント ジュード チルドレンズ リサーチ ホスピタル，インコーポレイティド 核酸増幅のための方法
JP2022543375A (ja) 2019-07-31 2022-10-12 バイオスクリブ ゲノミックス，インク． 遺伝子変異分析

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GONZALEZ-PENA, Veronica et al.，PNAS，2021年06月15日，118(24)，e2024176118

Also Published As

Publication number Publication date
WO2021022085A3 (en) 2021-03-11
EP4004201A4 (en) 2023-08-23
AU2020322027A1 (en) 2022-03-03
CA3149610A1 (en) 2021-02-04
CN114555802A (zh) 2022-05-27
KR20220041875A (ko) 2022-04-01
CN114555802B (zh) 2025-01-07
IL290245A (en) 2022-03-01
JP2022543051A (ja) 2022-10-07
MX2022001324A (es) 2022-05-19
EP4004201A2 (en) 2022-06-01
WO2021022085A2 (en) 2021-02-04
JP2025143255A (ja) 2025-10-01
US20230220377A1 (en) 2023-07-13

Similar Documents

Publication Publication Date Title
JP7691975B2 (ja) 単一細胞分析
US11643682B2 (en) Method for nucleic acid amplification
CA3149201A1 (en) Genetic mutational analysis
US20240368695A1 (en) Embryonic nucleic acid analysis
WO2023004058A1 (en) Spatial nucleic acid analysis
US20260002203A1 (en) Primary template-directed amplification and methods thereof
US20240316556A1 (en) High-throughput analysis of biomolecules
US20250230493A1 (en) Method for combined genome methylation and variation analyses
EP4594728A2 (en) Methods and compositions for fixed sample analysis
HK40042337A (en) Method for nucleic acid amplification
HK40042337B (en) Method for nucleic acid amplification

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221024

RD12 Notification of acceptance of power of sub attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7432

Effective date: 20221209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20221209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230728

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230728

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20240731

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240815

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20241113

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20250110

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250217

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250507

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250602

R150 Certificate of patent or registration of utility model

Ref document number: 7691975

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150