JP7691975B2 - 単一細胞分析 - Google Patents

単一細胞分析 Download PDF

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JP7691975B2
JP7691975B2 JP2022506428A JP2022506428A JP7691975B2 JP 7691975 B2 JP7691975 B2 JP 7691975B2 JP 2022506428 A JP2022506428 A JP 2022506428A JP 2022506428 A JP2022506428 A JP 2022506428A JP 7691975 B2 JP7691975 B2 JP 7691975B2
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JPWO2021022085A5 (https=
JP2022543051A (ja
JP2022543051A5 (https=
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ガワッド，チャールズ
エー．エー． ウェスト，ジェイ
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バイオスクリブ ゲノミックス，インク．
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2022506428A 2019-07-31 2020-07-30 単一細胞分析 Active JP7691975B2 (ja)

Priority Applications (1)

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JP2025090040A JP2025143255A (ja) 2019-07-31 2025-05-29 単一細胞分析

Applications Claiming Priority (3)

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US201962881183P 2019-07-31 2019-07-31
US62/881,183 2019-07-31
PCT/US2020/044338 WO2021022085A2 (en) 2019-07-31 2020-07-30 Single cell analysis

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JP2022543051A JP2022543051A (ja) 2022-10-07
JPWO2021022085A5 JPWO2021022085A5 (https=) 2023-08-08
JP2022543051A5 JP2022543051A5 (https=) 2023-08-08
JP7691975B2 true JP7691975B2 (ja) 2025-06-12

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US (1) US20230220377A1 (https=)
EP (1) EP4004201A4 (https=)
JP (2) JP7691975B2 (https=)
KR (1) KR20220041875A (https=)
CN (1) CN114555802B (https=)
AU (1) AU2020322027A1 (https=)
CA (1) CA3149610A1 (https=)
IL (1) IL290245A (https=)
MX (1) MX2022001324A (https=)
WO (1) WO2021022085A2 (https=)

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EA202091673A1 (ru) 2018-01-29 2021-02-03 Ст. Джуд Чилдрен'С Рисерч Хоспитал, Инк. Способ амплификации нуклеиновых кислот
EP4004230A4 (en) * 2019-07-31 2023-08-09 Bioskryb Genomics, Inc. GENETIC MUTATION ANALYSIS
WO2022195089A1 (en) * 2021-03-19 2022-09-22 Paris Sciences Et Lettres Methods for the selective analysis of cells or organelles
CN113046416B (zh) * 2021-03-24 2026-01-09 北京百迈客生物科技有限公司 一种纳米孔全长转录组文库构建方法
CN113151425B (zh) * 2021-04-08 2023-01-06 中国计量科学研究院 基于关键指标提高准确性的单细胞测序方法
WO2022232050A1 (en) * 2021-04-26 2022-11-03 The Broad Institute, Inc. Compositions and methods for characterizing polynucleotide sequence alterations
CN118284703A (zh) * 2021-08-16 2024-07-02 铂赛基因组学公司 胚胎核酸分析
WO2023055790A1 (en) * 2021-09-29 2023-04-06 Battelle Memorial Institute Apparatus and methods for performing multiple omics analysis and processing analyte mixtures
CN113943729B (zh) * 2021-10-20 2023-05-16 翌圣生物科技(上海)股份有限公司 U型接头及采用u型接头介导的磁珠偶联转座酶进行rna快速均一化建库的方法
CN119585427A (zh) * 2022-04-28 2025-03-07 铂赛基因组学公司 单细胞多组学
AU2023264552A1 (en) * 2022-05-05 2024-12-19 BioSkryb Genomics, Inc. Primary template-directed amplification and methods thereof
CN115144519A (zh) * 2022-06-30 2022-10-04 上海交通大学 基于无机纳米颗粒的单细胞样品指纹图谱检测方法和应用
WO2024026376A2 (en) * 2022-07-27 2024-02-01 BioSkryb Genomics, Inc. Methods and systems for multiomic analysis
CN115820802A (zh) * 2022-10-31 2023-03-21 北京百迈客生物科技有限公司 一种单细胞全长转录组文库的构建方法及其应用
CN120099141B (zh) * 2025-03-13 2026-03-06 江苏硕世生物科技股份有限公司 一种pcr长扩增子的二代测序文库构建方法及应用

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EP4004201A4 (en) 2023-08-23
WO2021022085A3 (en) 2021-03-11
IL290245A (en) 2022-03-01
WO2021022085A2 (en) 2021-02-04
CA3149610A1 (en) 2021-02-04
JP2025143255A (ja) 2025-10-01
US20230220377A1 (en) 2023-07-13
KR20220041875A (ko) 2022-04-01
AU2020322027A1 (en) 2022-03-03
JP2022543051A (ja) 2022-10-07
CN114555802A (zh) 2022-05-27
MX2022001324A (es) 2022-05-19
CN114555802B (zh) 2025-01-07
EP4004201A2 (en) 2022-06-01

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