IL290245A - Single cell analysis - Google Patents
Single cell analysisInfo
- Publication number
- IL290245A IL290245A IL290245A IL29024522A IL290245A IL 290245 A IL290245 A IL 290245A IL 290245 A IL290245 A IL 290245A IL 29024522 A IL29024522 A IL 29024522A IL 290245 A IL290245 A IL 290245A
- Authority
- IL
- Israel
- Prior art keywords
- single cell
- cell analysis
- analysis
- cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962881183P | 2019-07-31 | 2019-07-31 | |
| PCT/US2020/044338 WO2021022085A2 (en) | 2019-07-31 | 2020-07-30 | Single cell analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL290245A true IL290245A (en) | 2022-03-01 |
Family
ID=74228691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL290245A IL290245A (en) | 2019-07-31 | 2022-01-30 | Single cell analysis |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230220377A1 (https=) |
| EP (1) | EP4004201A4 (https=) |
| JP (2) | JP7691975B2 (https=) |
| KR (1) | KR20220041875A (https=) |
| CN (1) | CN114555802B (https=) |
| AU (1) | AU2020322027A1 (https=) |
| CA (1) | CA3149610A1 (https=) |
| IL (1) | IL290245A (https=) |
| MX (1) | MX2022001324A (https=) |
| WO (1) | WO2021022085A2 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2937927T3 (es) | 2018-01-29 | 2023-04-03 | St Jude Childrens Res Hospital Inc | Método para la amplificación de ácidos nucleicos |
| AU2020321370A1 (en) * | 2019-07-31 | 2022-03-03 | BioSkryb Genomics, Inc. | Genetic mutational analysis |
| US20240167082A1 (en) * | 2021-03-19 | 2024-05-23 | Paris Sciences Et Lettres | Methods for the selective analysis of cells or organelles |
| CN113046416B (zh) * | 2021-03-24 | 2026-01-09 | 北京百迈客生物科技有限公司 | 一种纳米孔全长转录组文库构建方法 |
| CN113151425B (zh) * | 2021-04-08 | 2023-01-06 | 中国计量科学研究院 | 基于关键指标提高准确性的单细胞测序方法 |
| JP2024516637A (ja) * | 2021-04-26 | 2024-04-16 | ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッド | ポリヌクレオチド配列の変化を特徴決定するための組成物および方法 |
| EP4388128A4 (en) * | 2021-08-16 | 2025-07-09 | Bioskryb Genomics Inc | EMBRYONIC NUCLEIC ACID ANALYSIS |
| US20230113026A1 (en) * | 2021-09-29 | 2023-04-13 | Battelle Memorial Institute | Apparatuses and Methods for Performing Multiple Omics Analysis and Processing Analyte Mixtures |
| CN113943729B (zh) * | 2021-10-20 | 2023-05-16 | 翌圣生物科技(上海)股份有限公司 | U型接头及采用u型接头介导的磁珠偶联转座酶进行rna快速均一化建库的方法 |
| WO2023212223A1 (en) * | 2022-04-28 | 2023-11-02 | BioSkryb Genomics, Inc. | Single cell multiomics |
| WO2023215524A2 (en) * | 2022-05-05 | 2023-11-09 | BioSkryb Genomics, Inc. | Primary template-directed amplification and methods thereof |
| CN115144519A (zh) * | 2022-06-30 | 2022-10-04 | 上海交通大学 | 基于无机纳米颗粒的单细胞样品指纹图谱检测方法和应用 |
| WO2024026376A2 (en) * | 2022-07-27 | 2024-02-01 | BioSkryb Genomics, Inc. | Methods and systems for multiomic analysis |
| CN115820802A (zh) * | 2022-10-31 | 2023-03-21 | 北京百迈客生物科技有限公司 | 一种单细胞全长转录组文库的构建方法及其应用 |
| CN120099141B (zh) * | 2025-03-13 | 2026-03-06 | 江苏硕世生物科技股份有限公司 | 一种pcr长扩增子的二代测序文库构建方法及应用 |
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| US5001050A (en) | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
| US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| WO1997014028A2 (en) | 1995-10-11 | 1997-04-17 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6485944B1 (en) | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| JP2001519538A (ja) | 1997-10-10 | 2001-10-23 | プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ | 核酸アレイのレプリカ増幅 |
| DE69812329T2 (de) | 1997-11-18 | 2004-02-12 | Bio-Rad Laboratories, Inc., Hercules | Multiplex-zufluss-immunotest mit magnetischen teilchen als festphase |
| US6565727B1 (en) | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
| US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
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| WO2001013119A1 (en) | 1999-08-17 | 2001-02-22 | Luminex Corporation | Encapsulation of fluorescent particles |
| AU2001280796A1 (en) | 2000-07-25 | 2002-02-05 | The Regents Of The University Of California | Electrowetting-driven micropumping |
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| US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
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| FR2841063B1 (fr) | 2002-06-18 | 2004-09-17 | Commissariat Energie Atomique | Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques |
| US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
| US7547380B2 (en) | 2003-01-13 | 2009-06-16 | North Carolina State University | Droplet transportation devices and methods having a fluid surface |
| JP4773360B2 (ja) | 2003-11-17 | 2011-09-14 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | 流体を操作するためのシステム |
| GB0400584D0 (en) | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
| EP1704402B1 (en) | 2004-01-14 | 2016-05-11 | Luminex Corporation | Methods and systems for dynamic range expansion |
| FR2866493B1 (fr) | 2004-02-16 | 2010-08-20 | Commissariat Energie Atomique | Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides |
| AU2005216549A1 (en) | 2004-02-27 | 2005-09-09 | President And Fellows Of Harvard College | Polony fluorescent in situ sequencing beads |
| FR2872715B1 (fr) | 2004-07-08 | 2006-11-17 | Commissariat Energie Atomique | Microreacteur goutte |
| FR2872809B1 (fr) | 2004-07-09 | 2006-09-15 | Commissariat Energie Atomique | Methode d'adressage d'electrodes |
| WO2006073504A2 (en) | 2004-08-04 | 2006-07-13 | President And Fellows Of Harvard College | Wobble sequencing |
| JP2006058031A (ja) | 2004-08-17 | 2006-03-02 | Hitachi High-Technologies Corp | 化学分析装置 |
| WO2006079016A1 (en) | 2005-01-20 | 2006-07-27 | Luminex Corporation | Magnetic microspheres for use in fluorescence-based applications |
| US7458661B2 (en) | 2005-01-25 | 2008-12-02 | The Regents Of The University Of California | Method and apparatus for promoting the complete transfer of liquid drops from a nozzle |
| DK1859330T3 (da) | 2005-01-28 | 2012-10-15 | Univ Duke | Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort |
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| WO2008055256A2 (en) | 2006-11-02 | 2008-05-08 | The Regents Of The University Of California | Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip |
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| WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| US20080269068A1 (en) | 2007-02-06 | 2008-10-30 | President And Fellows Of Harvard College | Multiplex decoding of sequence tags in barcodes |
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| US9309511B2 (en) * | 2007-08-28 | 2016-04-12 | The Johns Hopkins University | Functional assay for identification of loss-of-function mutations in genes |
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| US10480021B2 (en) * | 2014-06-23 | 2019-11-19 | Yale University | Methods for closed chromatin mapping and DNA methylation analysis for single cells |
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| CN109791157B (zh) * | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| CA3047328A1 (en) * | 2016-12-21 | 2018-06-28 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
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| KR20190133711A (ko) * | 2017-03-24 | 2019-12-03 | 싱가포르국립대학교 | 분자의 다중 검출 방법 |
| DK3635136T3 (da) | 2017-06-07 | 2022-01-10 | Univ Oregon Health & Science | Enkeltcelle-helgenombiblioteker til methyleringssekvensering |
| SG11201911869XA (en) * | 2017-08-01 | 2020-01-30 | Illumina Inc | Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells |
| WO2019084055A1 (en) * | 2017-10-23 | 2019-05-02 | Massachusetts Institute Of Technology | CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS |
| WO2019121842A1 (en) * | 2017-12-21 | 2019-06-27 | F. Hoffmann-La Roche Ag | Target enrichment by unidirectional dual probe primer extension |
| ES2937927T3 (es) * | 2018-01-29 | 2023-04-03 | St Jude Childrens Res Hospital Inc | Método para la amplificación de ácidos nucleicos |
| AU2020321370A1 (en) * | 2019-07-31 | 2022-03-03 | BioSkryb Genomics, Inc. | Genetic mutational analysis |
-
2020
- 2020-07-30 MX MX2022001324A patent/MX2022001324A/es unknown
- 2020-07-30 US US17/631,130 patent/US20230220377A1/en active Pending
- 2020-07-30 AU AU2020322027A patent/AU2020322027A1/en active Pending
- 2020-07-30 JP JP2022506428A patent/JP7691975B2/ja active Active
- 2020-07-30 WO PCT/US2020/044338 patent/WO2021022085A2/en not_active Ceased
- 2020-07-30 CA CA3149610A patent/CA3149610A1/en active Pending
- 2020-07-30 EP EP20847237.3A patent/EP4004201A4/en active Pending
- 2020-07-30 CN CN202080069560.6A patent/CN114555802B/zh active Active
- 2020-07-30 KR KR1020227006454A patent/KR20220041875A/ko active Pending
-
2022
- 2022-01-30 IL IL290245A patent/IL290245A/en unknown
-
2025
- 2025-05-29 JP JP2025090040A patent/JP2025143255A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021022085A3 (en) | 2021-03-11 |
| EP4004201A4 (en) | 2023-08-23 |
| AU2020322027A1 (en) | 2022-03-03 |
| CA3149610A1 (en) | 2021-02-04 |
| CN114555802A (zh) | 2022-05-27 |
| KR20220041875A (ko) | 2022-04-01 |
| CN114555802B (zh) | 2025-01-07 |
| JP2022543051A (ja) | 2022-10-07 |
| JP7691975B2 (ja) | 2025-06-12 |
| MX2022001324A (es) | 2022-05-19 |
| EP4004201A2 (en) | 2022-06-01 |
| WO2021022085A2 (en) | 2021-02-04 |
| JP2025143255A (ja) | 2025-10-01 |
| US20230220377A1 (en) | 2023-07-13 |
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