MX2022001324A - Análisis de células individuales. - Google Patents

Análisis de células individuales.

Info

Publication number
MX2022001324A
MX2022001324A MX2022001324A MX2022001324A MX2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A
Authority
MX
Mexico
Prior art keywords
methods
analysis
sequencing
single cell
cells
Prior art date
Application number
MX2022001324A
Other languages
English (en)
Spanish (es)
Inventor
Charles Gawad
Jay A A West
Original Assignee
Bioskryb Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioskryb Genomics Inc filed Critical Bioskryb Genomics Inc
Publication of MX2022001324A publication Critical patent/MX2022001324A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
MX2022001324A 2019-07-31 2020-07-30 Análisis de células individuales. MX2022001324A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962881183P 2019-07-31 2019-07-31
PCT/US2020/044338 WO2021022085A2 (en) 2019-07-31 2020-07-30 Single cell analysis

Publications (1)

Publication Number Publication Date
MX2022001324A true MX2022001324A (es) 2022-05-19

Family

ID=74228691

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022001324A MX2022001324A (es) 2019-07-31 2020-07-30 Análisis de células individuales.

Country Status (10)

Country Link
US (1) US20230220377A1 (https=)
EP (1) EP4004201A4 (https=)
JP (2) JP7691975B2 (https=)
KR (1) KR20220041875A (https=)
CN (1) CN114555802B (https=)
AU (1) AU2020322027A1 (https=)
CA (1) CA3149610A1 (https=)
IL (1) IL290245A (https=)
MX (1) MX2022001324A (https=)
WO (1) WO2021022085A2 (https=)

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CN113151425B (zh) * 2021-04-08 2023-01-06 中国计量科学研究院 基于关键指标提高准确性的单细胞测序方法
JP2024516637A (ja) * 2021-04-26 2024-04-16 ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッド ポリヌクレオチド配列の変化を特徴決定するための組成物および方法
EP4388128A4 (en) * 2021-08-16 2025-07-09 Bioskryb Genomics Inc EMBRYONIC NUCLEIC ACID ANALYSIS
US20230113026A1 (en) * 2021-09-29 2023-04-13 Battelle Memorial Institute Apparatuses and Methods for Performing Multiple Omics Analysis and Processing Analyte Mixtures
CN113943729B (zh) * 2021-10-20 2023-05-16 翌圣生物科技(上海)股份有限公司 U型接头及采用u型接头介导的磁珠偶联转座酶进行rna快速均一化建库的方法
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CN115144519A (zh) * 2022-06-30 2022-10-04 上海交通大学 基于无机纳米颗粒的单细胞样品指纹图谱检测方法和应用
WO2024026376A2 (en) * 2022-07-27 2024-02-01 BioSkryb Genomics, Inc. Methods and systems for multiomic analysis
CN115820802A (zh) * 2022-10-31 2023-03-21 北京百迈客生物科技有限公司 一种单细胞全长转录组文库的构建方法及其应用
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Also Published As

Publication number Publication date
WO2021022085A3 (en) 2021-03-11
EP4004201A4 (en) 2023-08-23
AU2020322027A1 (en) 2022-03-03
CA3149610A1 (en) 2021-02-04
CN114555802A (zh) 2022-05-27
KR20220041875A (ko) 2022-04-01
CN114555802B (zh) 2025-01-07
IL290245A (en) 2022-03-01
JP2022543051A (ja) 2022-10-07
JP7691975B2 (ja) 2025-06-12
EP4004201A2 (en) 2022-06-01
WO2021022085A2 (en) 2021-02-04
JP2025143255A (ja) 2025-10-01
US20230220377A1 (en) 2023-07-13

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