JP7457457B2 - サンプル中の標的核酸を検出する方法 - Google Patents
サンプル中の標的核酸を検出する方法 Download PDFInfo
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- JP7457457B2 JP7457457B2 JP2018560101A JP2018560101A JP7457457B2 JP 7457457 B2 JP7457457 B2 JP 7457457B2 JP 2018560101 A JP2018560101 A JP 2018560101A JP 2018560101 A JP2018560101 A JP 2018560101A JP 7457457 B2 JP7457457 B2 JP 7457457B2
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- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/30—Characterised by physical treatment
- C12Q2523/319—Photocleavage, photolysis, photoactivation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022089840A JP2022122964A (ja) | 2016-05-16 | 2022-06-01 | サンプル中の標的核酸を検出する方法 |
| JP2024143175A JP2024161084A (ja) | 2016-05-16 | 2024-08-23 | サンプル中の標的核酸を検出する方法 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662337074P | 2016-05-16 | 2016-05-16 | |
| US62/337,074 | 2016-05-16 | ||
| US201762492889P | 2017-05-01 | 2017-05-01 | |
| US62/492,889 | 2017-05-01 | ||
| PCT/US2017/032947 WO2017201073A1 (en) | 2016-05-16 | 2017-05-16 | Methods for detecting target nucleic acids in a sample |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022089840A Division JP2022122964A (ja) | 2016-05-16 | 2022-06-01 | サンプル中の標的核酸を検出する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019522463A JP2019522463A (ja) | 2019-08-15 |
| JP2019522463A5 JP2019522463A5 (enExample) | 2020-06-18 |
| JP7457457B2 true JP7457457B2 (ja) | 2024-03-28 |
Family
ID=58772988
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018560101A Active JP7457457B2 (ja) | 2016-05-16 | 2017-05-16 | サンプル中の標的核酸を検出する方法 |
| JP2022089840A Pending JP2022122964A (ja) | 2016-05-16 | 2022-06-01 | サンプル中の標的核酸を検出する方法 |
| JP2024143175A Pending JP2024161084A (ja) | 2016-05-16 | 2024-08-23 | サンプル中の標的核酸を検出する方法 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022089840A Pending JP2022122964A (ja) | 2016-05-16 | 2022-06-01 | サンプル中の標的核酸を検出する方法 |
| JP2024143175A Pending JP2024161084A (ja) | 2016-05-16 | 2024-08-23 | サンプル中の標的核酸を検出する方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US20170327876A1 (enExample) |
| EP (2) | EP4324929B1 (enExample) |
| JP (3) | JP7457457B2 (enExample) |
| KR (1) | KR102490693B1 (enExample) |
| CN (2) | CN116200462B (enExample) |
| AU (2) | AU2017268257B2 (enExample) |
| ES (1) | ES2956732T3 (enExample) |
| SG (1) | SG11201809913PA (enExample) |
| WO (1) | WO2017201073A1 (enExample) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014531908A (ja) | 2011-10-14 | 2014-12-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 構造アッセンブリによる配列決定 |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| EP4273263A3 (en) | 2014-07-30 | 2024-01-31 | President and Fellows of Harvard College | Systems and methods for determining nucleic acids |
| SG11201707515SA (en) | 2015-04-10 | 2017-10-30 | Spatial Transcriptomics Ab | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| EP3449016B1 (en) | 2016-04-25 | 2025-07-23 | President and Fellows of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| EP3472359B1 (en) | 2016-06-21 | 2022-03-16 | 10X Genomics, Inc. | Nucleic acid sequencing |
| US10415080B2 (en) * | 2016-11-21 | 2019-09-17 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
| KR102145417B1 (ko) * | 2017-05-24 | 2020-08-19 | 지니너스 주식회사 | 무세포 핵산으로부터 수득된 서열 분석 데이터에 대한 배경 대립인자의 빈도 분포를 생성하는 방법 및 이를 이용하여 무세포 핵산으로부터 변이를 검출하는 방법 |
| US11788123B2 (en) | 2017-05-26 | 2023-10-17 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
| CN120210336A (zh) | 2017-10-06 | 2025-06-27 | 10X基因组学有限公司 | Rna模板化连接 |
| US10871485B2 (en) | 2018-04-13 | 2020-12-22 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| KR102893736B1 (ko) | 2018-05-14 | 2025-11-28 | 브루커 스페이셜 바이올로지, 인크. | 화학 조성물 및 이의 사용 방법 |
| WO2019241250A1 (en) | 2018-06-11 | 2019-12-19 | Foundation Medicine, Inc. | Compositions and methods for evaluating genomic alterations |
| US20210223254A1 (en) * | 2018-07-09 | 2021-07-22 | Ultivue, Inc. | Methods for Multicolor Multiplex Imaging |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN117778535A (zh) * | 2018-10-25 | 2024-03-29 | Illumina公司 | 使用索引和条形码识别阵列上的配体的方法和组合物 |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| WO2020123742A1 (en) * | 2018-12-13 | 2020-06-18 | President And Fellows Of Harvard College | Amplification methods and systems for merfish and other applications |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| WO2020240025A1 (en) | 2019-05-31 | 2020-12-03 | Cartana Ab | Method of detecting target nucleic acid molecules |
| KR102336454B1 (ko) * | 2019-06-17 | 2021-12-08 | 강릉원주대학교산학협력단 | 핵산 검출 방법 |
| WO2020254672A1 (en) | 2019-06-19 | 2020-12-24 | Therycell Gmbh | Spatial characterisation of target structures in a sample |
| JP2022538835A (ja) * | 2019-07-01 | 2022-09-06 | ダヴィンチ バイオ、インコーポレイテッド | 高容量分子検出 |
| WO2021066464A2 (ko) * | 2019-10-04 | 2021-04-08 | 프로지니어 주식회사 | 핵산 검출 시스템 |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| US20230029257A1 (en) * | 2019-12-12 | 2023-01-26 | President And Fellows Of Harvard College | Compositions and methods for light-directed biomolecular barcoding |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| CN115038794A (zh) | 2019-12-23 | 2022-09-09 | 10X基因组学有限公司 | 在基于分区的测定中使用固定生物样品的组合物和方法 |
| CN121022991A (zh) | 2019-12-23 | 2025-11-28 | 10X基因组学有限公司 | 使用rna模板化连接进行空间分析的方法 |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| CN115485391A (zh) | 2020-02-17 | 2022-12-16 | 10X基因组学有限公司 | 染色质相互作用的原位分析 |
| EP4107285B1 (en) | 2020-02-21 | 2024-10-09 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
| WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| ES2989052T3 (es) | 2020-05-22 | 2024-11-25 | 10X Genomics Inc | Medición espacio-temporal simultánea de la expresión génica y la actividad celular |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| ES2981265T3 (es) | 2020-06-08 | 2024-10-08 | 10X Genomics Inc | Métodos para determinar un margen quirúrgico y métodos de uso del mismo |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| EP4168575A1 (en) | 2020-06-18 | 2023-04-26 | Resolve BioSciences GmbH | Multiplex method for detecting different analytes in a sample |
| EP4172362B1 (en) | 2020-06-25 | 2024-09-18 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4121555A1 (en) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| EP4320270A1 (en) * | 2021-04-06 | 2024-02-14 | Akoya Biosciences, Inc. | Detection of labeled analytes in biological samples |
| EP4428246B1 (en) | 2021-04-14 | 2025-12-24 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| WO2022236054A1 (en) | 2021-05-06 | 2022-11-10 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| US20240240237A1 (en) * | 2021-05-24 | 2024-07-18 | California Institute Of Technology | Linked amplification tethered with exponential radiance |
| EP4582555A3 (en) | 2021-06-03 | 2025-10-22 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| CA3224093A1 (en) | 2021-07-30 | 2023-02-02 | Jorge Ivan Hernandez Neuta | Methods and compositions for synchronizing reactions in situ |
| US12460251B2 (en) | 2021-08-03 | 2025-11-04 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid molecules |
| WO2023014825A1 (en) * | 2021-08-03 | 2023-02-09 | Akoya Biosciences, Inc. | Rna detection by selective labeling and amplification |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| EP4326898B1 (en) | 2021-08-16 | 2024-07-31 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| EP4419707A1 (en) | 2021-11-10 | 2024-08-28 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| CN118974836A (zh) | 2022-03-08 | 2024-11-15 | 10X基因组学有限公司 | 将光学拥挤最小化的原位代码设计方法 |
| AU2023237896A1 (en) | 2022-03-24 | 2024-10-17 | Digital Biology Inc. | Tissue spatial omics |
| AU2023243205A1 (en) | 2022-04-01 | 2024-09-19 | 10X Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| JP2025516589A (ja) | 2022-05-11 | 2025-05-30 | 10エックス ジェノミクス インコーポレイテッド | インサイチュー配列決定のための組成物及び方法 |
| US20240035071A1 (en) | 2022-06-17 | 2024-02-01 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| CN119677873A (zh) | 2022-08-16 | 2025-03-21 | 10X基因组学有限公司 | Ap50聚合酶及其用途 |
| EP4662234A2 (en) * | 2023-02-08 | 2025-12-17 | Toreador Therapeutics, Inc. | Super-resolution microscopy (srm) multiplexing with cleavable moieties |
| WO2024196764A2 (en) | 2023-03-17 | 2024-09-26 | Nanostring Technologies, Inc. | Assay for recombinase accessible chromatin and related compositions and methods |
| WO2024196728A2 (en) | 2023-03-17 | 2024-09-26 | Nanostring Technologies, Inc. | Nucleic acid probes for combined sequencing and spatial analysis |
| WO2025029831A1 (en) | 2023-07-31 | 2025-02-06 | 10X Genomics, Inc. | Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060088872A1 (en) | 2003-04-24 | 2006-04-27 | Afshin Ahmadian | Allele-specific mutation detection assay |
| WO2013055995A2 (en) | 2011-10-14 | 2013-04-18 | President And Fellows Of Harvard College | Sequencing by structure assembly |
Family Cites Families (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4230558A (en) | 1978-10-02 | 1980-10-28 | Coulter Electronics, Inc. | Single drop separator |
| US4318846A (en) | 1979-09-07 | 1982-03-09 | Syva Company | Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers |
| US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
| US5091519A (en) | 1986-05-01 | 1992-02-25 | Amoco Corporation | Nucleotide compositions with linking groups |
| US4811218A (en) | 1986-06-02 | 1989-03-07 | Applied Biosystems, Inc. | Real time scanning electrophoresis apparatus for DNA sequencing |
| US5151507A (en) | 1986-07-02 | 1992-09-29 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
| US5066580A (en) | 1988-08-31 | 1991-11-19 | Becton Dickinson And Company | Xanthene dyes that emit to the red of fluorescein |
| US5366860A (en) | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
| US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
| US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| US5800996A (en) | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
| US5847162A (en) | 1996-06-27 | 1998-12-08 | The Perkin Elmer Corporation | 4, 7-Dichlororhodamine dyes |
| US5853993A (en) * | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| US6322901B1 (en) | 1997-11-13 | 2001-11-27 | Massachusetts Institute Of Technology | Highly luminescent color-selective nano-crystalline materials |
| US6207392B1 (en) | 1997-11-25 | 2001-03-27 | The Regents Of The University Of California | Semiconductor nanocrystal probes for biological applications and process for making and using such probes |
| US5990479A (en) | 1997-11-25 | 1999-11-23 | Regents Of The University Of California | Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes |
| US6251303B1 (en) | 1998-09-18 | 2001-06-26 | Massachusetts Institute Of Technology | Water-soluble fluorescent nanocrystals |
| US6426513B1 (en) | 1998-09-18 | 2002-07-30 | Massachusetts Institute Of Technology | Water-soluble thiol-capped nanocrystals |
| EP1961826A3 (en) * | 1999-03-10 | 2008-09-17 | ASM Scientific, Inc. | A method for direct nucleic acid sequencing |
| US6649138B2 (en) | 2000-10-13 | 2003-11-18 | Quantum Dot Corporation | Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media |
| US6576291B2 (en) | 2000-12-08 | 2003-06-10 | Massachusetts Institute Of Technology | Preparation of nanocrystallites |
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| ATE556845T1 (de) | 2001-07-20 | 2012-05-15 | Life Technologies Corp | Lumineszierende nanopartikel und ihre herstellung |
| US7057026B2 (en) * | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US20060210982A1 (en) * | 2003-01-31 | 2006-09-21 | Hiroshi Yanagawa | Cleavable assigned molecules and screening method using the same |
| CN101189345A (zh) * | 2005-02-01 | 2008-05-28 | Ab先进基因分析公司 | 珠基测序的试剂、方法和文库 |
| US20090299640A1 (en) | 2005-11-23 | 2009-12-03 | University Of Utah Research Foundation | Methods and Compositions Involving Intrinsic Genes |
| WO2007070869A2 (en) * | 2005-12-16 | 2007-06-21 | Applera Corporation | Method and system for phase-locked sequencing |
| AU2006330830B2 (en) | 2005-12-23 | 2013-01-10 | Bruker Spatial Biology, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| EP1963500B1 (en) | 2005-12-23 | 2013-02-13 | Nanostring Technologies, Inc. | Compositions comprising oriented, immobilized macromolecules and methods for their preparation |
| ES2620398T3 (es) | 2006-05-22 | 2017-06-28 | Nanostring Technologies, Inc. | Sistemas y métodos para analizar nanoindicadores |
| US7745129B1 (en) * | 2006-07-12 | 2010-06-29 | Kenneth David Schatz | Methods for sequencing of a necleic acid |
| EP2099928B1 (en) * | 2006-12-01 | 2016-01-27 | Affymetrix, Inc. | Two-stage nucleic acid amplification using an amplification oligomer |
| US8415102B2 (en) | 2007-04-10 | 2013-04-09 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
| WO2009046149A1 (en) * | 2007-10-01 | 2009-04-09 | Applied Biosystems Inc. | Chase ligation sequencing |
| SG193776A1 (en) * | 2007-12-05 | 2013-10-30 | Complete Genomics Inc | Efficient base determination in sequencing reactions |
| US20090298709A1 (en) * | 2008-05-28 | 2009-12-03 | Affymetrix, Inc. | Assays for determining telomere length and repeated sequence copy number |
| US9631239B2 (en) | 2008-05-30 | 2017-04-25 | University Of Utah Research Foundation | Method of classifying a breast cancer instrinsic subtype |
| GB2461026B (en) * | 2008-06-16 | 2011-03-09 | Plc Diagnostics Inc | System and method for nucleic acids sequencing by phased synthesis |
| US8519115B2 (en) | 2008-08-14 | 2013-08-27 | Nanostring Technologies, Inc. | Stable nanoreporters |
| WO2011032040A1 (en) * | 2009-09-10 | 2011-03-17 | Centrillion Technology Holding Corporation | Methods of targeted sequencing |
| WO2011047087A2 (en) | 2009-10-13 | 2011-04-21 | Nanostring Technologies, Inc. | Protein detection via nanoreporters |
| JP5914362B2 (ja) | 2010-02-11 | 2016-05-11 | ナノストリング テクノロジーズ, インコーポレイテッド | 低分子rna分子の検出のための組成物および方法 |
| ES2606012T3 (es) * | 2010-02-26 | 2017-03-17 | Ventana Medical Systems, Inc. | Hibridación in situ con sondas PolyTag |
| US20110229888A1 (en) | 2010-03-16 | 2011-09-22 | Hengen Paul N | Compositions and Methods for the Detection of Genomic Features |
| US20120003648A1 (en) * | 2010-07-01 | 2012-01-05 | Affymetrix, Inc. | Signal Multiplexing and Signal Amplification |
| EP2685988A4 (en) | 2011-03-15 | 2014-08-20 | Univ North Carolina | Methods of treating breast cancer with anthracycline therapy |
| US9758834B2 (en) | 2011-03-28 | 2017-09-12 | Nanostring Technologies, Inc. | Compositions and methods for diagnosing cancer |
| WO2012157684A1 (ja) * | 2011-05-16 | 2012-11-22 | 地方独立行政法人 大阪府立病院機構 | 血中dnaの定量的検出による悪性新生物の病勢の進行を評価する方法 |
| WO2012173274A1 (ja) * | 2011-06-16 | 2012-12-20 | 日鉄環境エンジニアリング株式会社 | 核酸測定用の核酸プローブ |
| AU2012272763B2 (en) | 2011-06-24 | 2016-11-03 | Nanostring Technologies, Inc. | Multivariate diagnostic assays and methods for using same |
| AU2012345789B2 (en) | 2011-11-30 | 2018-02-15 | British Columbia Cancer Agency Branch | Methods of treating breast cancer with taxane therapy |
| IN2014MN02418A (enExample) | 2012-05-22 | 2015-08-14 | Nanostring Technologies Inc | |
| JP2015530072A (ja) | 2012-06-29 | 2015-10-15 | ナノストリング テクノロジーズ,インコーポレイティド | ゲムシタビン療法による乳癌の治療方法 |
| WO2014075067A1 (en) | 2012-11-12 | 2014-05-15 | Nanostring Technologies, Inc. | Methods to predict breast cancer outcome |
| WO2014182598A1 (en) * | 2013-05-06 | 2014-11-13 | Physicians Choice Laboratory Services, Llc | Diagnostic assay combining cellular and cell free nucleic acid |
| EP3008209A2 (en) * | 2013-06-14 | 2016-04-20 | Nanostring Technologies, Inc | Multiplexable tag-based reporter system |
| EP3221469B1 (en) * | 2014-11-21 | 2020-01-15 | Nanostring Technologies, Inc | Enzyme- and amplification-free sequencing |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060088872A1 (en) | 2003-04-24 | 2006-04-27 | Afshin Ahmadian | Allele-specific mutation detection assay |
| WO2013055995A2 (en) | 2011-10-14 | 2013-04-18 | President And Fellows Of Harvard College | Sequencing by structure assembly |
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| CN109715825A (zh) | 2019-05-03 |
| CN116200462B (zh) | 2025-10-03 |
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