JP7418210B2 - 遺伝学的にコード化可能なバイオセンサーのための頑強な小分子結合アプタマーを生成するためのインビトロ選択を用いた生物学的rna足場の使用 - Google Patents
遺伝学的にコード化可能なバイオセンサーのための頑強な小分子結合アプタマーを生成するためのインビトロ選択を用いた生物学的rna足場の使用 Download PDFInfo
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- JP7418210B2 JP7418210B2 JP2019531196A JP2019531196A JP7418210B2 JP 7418210 B2 JP7418210 B2 JP 7418210B2 JP 2019531196 A JP2019531196 A JP 2019531196A JP 2019531196 A JP2019531196 A JP 2019531196A JP 7418210 B2 JP7418210 B2 JP 7418210B2
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
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- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
- C12N2330/31—Libraries, arrays
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- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
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- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023064371A JP2023093572A (ja) | 2016-12-12 | 2023-04-11 | 遺伝学的にコード化可能なバイオセンサーのための頑強な小分子結合アプタマーを生成するためのインビトロ選択を用いた生物学的rna足場の使用 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662432879P | 2016-12-12 | 2016-12-12 | |
| US62/432,879 | 2016-12-12 | ||
| PCT/US2017/065526 WO2018111745A1 (en) | 2016-12-12 | 2017-12-11 | Use of biological rna scaffolds with in vitro selection to generate robust small molecule binding aptamers for genetically encodable biosensors |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023064371A Division JP2023093572A (ja) | 2016-12-12 | 2023-04-11 | 遺伝学的にコード化可能なバイオセンサーのための頑強な小分子結合アプタマーを生成するためのインビトロ選択を用いた生物学的rna足場の使用 |
Publications (3)
| Publication Number | Publication Date |
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| JP2020504096A JP2020504096A (ja) | 2020-02-06 |
| JP2020504096A5 JP2020504096A5 (enExample) | 2021-01-28 |
| JP7418210B2 true JP7418210B2 (ja) | 2024-01-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019531196A Active JP7418210B2 (ja) | 2016-12-12 | 2017-12-11 | 遺伝学的にコード化可能なバイオセンサーのための頑強な小分子結合アプタマーを生成するためのインビトロ選択を用いた生物学的rna足場の使用 |
| JP2023064371A Pending JP2023093572A (ja) | 2016-12-12 | 2023-04-11 | 遺伝学的にコード化可能なバイオセンサーのための頑強な小分子結合アプタマーを生成するためのインビトロ選択を用いた生物学的rna足場の使用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
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| JP2023064371A Pending JP2023093572A (ja) | 2016-12-12 | 2023-04-11 | 遺伝学的にコード化可能なバイオセンサーのための頑強な小分子結合アプタマーを生成するためのインビトロ選択を用いた生物学的rna足場の使用 |
Country Status (14)
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| EP (1) | EP3551755A4 (enExample) |
| JP (2) | JP7418210B2 (enExample) |
| KR (1) | KR102612226B1 (enExample) |
| CN (1) | CN110462039A (enExample) |
| AU (1) | AU2017378078B2 (enExample) |
| BR (1) | BR112019012012A2 (enExample) |
| CA (1) | CA3056218A1 (enExample) |
| EA (1) | EA201991446A1 (enExample) |
| IL (1) | IL267216B2 (enExample) |
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| MY (1) | MY205553A (enExample) |
| PH (1) | PH12019501302A1 (enExample) |
| WO (1) | WO2018111745A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10852274B2 (en) | 2017-03-09 | 2020-12-01 | Auburn University | Differential circuit for background correction in electrochemical measurements |
| US11505799B2 (en) | 2017-07-07 | 2022-11-22 | Innamed, Inc. | Aptamers for measuring lipoprotein levels |
| US11560565B2 (en) | 2018-06-13 | 2023-01-24 | Auburn University | Electrochemical detection nanostructure, systems, and uses thereof |
| WO2020264444A2 (en) * | 2019-06-27 | 2020-12-30 | Innamed, Inc. | Assay method for point of care quantification of an immunophilin-binding immunosuppressant drug |
| CN113430203A (zh) * | 2020-03-23 | 2021-09-24 | 华东理工大学 | 一种新型rna检测与定量的方法 |
| WO2021256582A1 (ko) * | 2020-06-17 | 2021-12-23 | 포항공과대학교 산학협력단 | 전사 활성 인자 및 토홀드 스위치를 포함하는 트립토판 검출용 바이오센서 |
| WO2022006042A1 (en) * | 2020-06-29 | 2022-01-06 | President And Fellows Of Harvard College | Methods, compositions, and kits for nucleic acid barcoding of biomolecules |
| CN115177740A (zh) * | 2021-04-01 | 2022-10-14 | 安升(上海)医药科技有限公司 | 核酸自组装介导的adc药物的构建方法及应用 |
| CN113512089B (zh) * | 2021-06-30 | 2023-06-13 | 兰州大学 | 一种水溶性量子点的多肽稳定剂及应用 |
| CN115704029A (zh) * | 2021-08-06 | 2023-02-17 | 纳莹(上海)生物科技有限公司 | 一种rna检测与定量的方法 |
| CN114518359B (zh) * | 2022-03-08 | 2024-08-20 | 山东理工大学 | 一种基于g-四联体的双模式卡那霉素适配体传感器的制备方法 |
| CN115791726B (zh) * | 2022-11-25 | 2024-11-15 | 海南大学 | 一种基于g-四联体dna超灵敏高选择性检测锶离子的方法 |
| WO2024118806A1 (en) * | 2022-11-29 | 2024-06-06 | The Johns Hopkins University | Aptamer regulated transcription for in-vitro sensing and transduction |
| CN116170470B (zh) * | 2023-02-28 | 2023-07-04 | 北京科易为科技有限公司 | 移液器系统的数据通讯方法及其通讯系统 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008518630A (ja) | 2004-11-08 | 2008-06-05 | イェール ユニバーシティ | リボスイッチ、リボスイッチによる、構造を基礎とする化合物設計、ならびにリボスイッチの使用方法およびリボスイッチを伴う組成物 |
| WO2013025930A1 (en) | 2011-08-16 | 2013-02-21 | Research Foundation of State University of New York at Albany | Aptamer modulators of estrogen receptors |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060121510A1 (en) * | 1998-11-03 | 2006-06-08 | Breaker Ronald R | Multidomain polynucleotide molecular sensors |
| US20030162190A1 (en) * | 2001-11-15 | 2003-08-28 | Gorenstein David G. | Phosphoromonothioate and phosphorodithioate oligonucleotide aptamer chip for functional proteomics |
| US20060121466A1 (en) * | 2002-06-13 | 2006-06-08 | Amgen Inc. | Hammerhead ribozymes |
| US20050019916A1 (en) * | 2003-04-14 | 2005-01-27 | Stojanovic Milan N. | Cross reactive arrays of three-way junction sensors for steriod determination |
| SG174103A1 (en) * | 2007-05-29 | 2011-09-29 | Univ Yale | Riboswitches and methods and compositions for use of and with riboswitches |
| WO2012021554A1 (en) * | 2010-08-09 | 2012-02-16 | Yale University | Cyclic di-gmp-ii riboswitches, motifs, and compounds, and methods for their use |
| US8383794B2 (en) * | 2010-10-08 | 2013-02-26 | Li Niu | Conformation-selective nucleic acid inhibitors of AMPA glutamate receptors |
| US20120248666A1 (en) * | 2011-03-30 | 2012-10-04 | Firestone Industrial Products Company, Llc | Gas spring and damper assembly and suspension system including same |
| US9664676B2 (en) * | 2013-09-06 | 2017-05-30 | Cornell University | RNA sequences that induce fluorescence of small molecule fluorophores |
| CN104293793B (zh) * | 2014-07-24 | 2018-05-18 | 江南大学 | 一种特异识别t-2毒素的寡核苷酸适配体 |
-
2017
- 2017-12-11 WO PCT/US2017/065526 patent/WO2018111745A1/en not_active Ceased
- 2017-12-11 CA CA3056218A patent/CA3056218A1/en active Pending
- 2017-12-11 EP EP17880409.2A patent/EP3551755A4/en active Pending
- 2017-12-11 IL IL267216A patent/IL267216B2/en unknown
- 2017-12-11 CN CN201780085607.6A patent/CN110462039A/zh active Pending
- 2017-12-11 BR BR112019012012-2A patent/BR112019012012A2/pt active Search and Examination
- 2017-12-11 KR KR1020197019421A patent/KR102612226B1/ko active Active
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- 2017-12-11 MX MX2019006825A patent/MX2019006825A/es unknown
- 2017-12-11 JP JP2019531196A patent/JP7418210B2/ja active Active
- 2017-12-11 MY MYPI2019003292A patent/MY205553A/en unknown
- 2017-12-11 EA EA201991446A patent/EA201991446A1/ru unknown
- 2017-12-11 US US16/468,945 patent/US20200208142A1/en not_active Abandoned
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2019
- 2019-06-10 PH PH12019501302A patent/PH12019501302A1/en unknown
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2021
- 2021-12-02 US US17/541,068 patent/US20220170009A1/en active Pending
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2023
- 2023-04-11 JP JP2023064371A patent/JP2023093572A/ja active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008518630A (ja) | 2004-11-08 | 2008-06-05 | イェール ユニバーシティ | リボスイッチ、リボスイッチによる、構造を基礎とする化合物設計、ならびにリボスイッチの使用方法およびリボスイッチを伴う組成物 |
| WO2013025930A1 (en) | 2011-08-16 | 2013-02-21 | Research Foundation of State University of New York at Albany | Aptamer modulators of estrogen receptors |
Non-Patent Citations (6)
| Title |
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| Biochim. Biophys. Acta,2014年,Vol. 1839,p. 919-930 |
| Front. Chem.,2016年06月,Vol. 4, Article 29,p. 1-12 |
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| Sci. Rep.,2016年10月,Vol. 6:34998,p. 1-13 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3551755A4 (en) | 2020-06-24 |
| JP2023093572A (ja) | 2023-07-04 |
| JP2020504096A (ja) | 2020-02-06 |
| MY205553A (en) | 2024-10-25 |
| IL267216B2 (en) | 2025-04-01 |
| US20200208142A1 (en) | 2020-07-02 |
| AU2017378078B2 (en) | 2024-06-13 |
| EP3551755A1 (en) | 2019-10-16 |
| KR102612226B1 (ko) | 2023-12-12 |
| PH12019501302A1 (en) | 2019-10-28 |
| EA201991446A1 (ru) | 2020-03-03 |
| CN110462039A (zh) | 2019-11-15 |
| BR112019012012A2 (pt) | 2019-10-29 |
| MX2019006825A (es) | 2019-10-30 |
| WO2018111745A1 (en) | 2018-06-21 |
| AU2017378078A1 (en) | 2019-07-25 |
| KR20190100227A (ko) | 2019-08-28 |
| IL267216A (en) | 2019-08-29 |
| CA3056218A1 (en) | 2018-06-21 |
| IL267216B1 (en) | 2024-12-01 |
| US20220170009A1 (en) | 2022-06-02 |
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