EA201991446A1 - Использование биологических рнк-остовов с селекцией in vitro для получения эффективных и надежных аптамеров, связывающих малые молекулы, для генетически кодируемых биосенсоров - Google Patents

Использование биологических рнк-остовов с селекцией in vitro для получения эффективных и надежных аптамеров, связывающих малые молекулы, для генетически кодируемых биосенсоров

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EA201991446A1
EA201991446A1 EA201991446A EA201991446A EA201991446A1 EA 201991446 A1 EA201991446 A1 EA 201991446A1 EA 201991446 A EA201991446 A EA 201991446A EA 201991446 A EA201991446 A EA 201991446A EA 201991446 A1 EA201991446 A1 EA 201991446A1
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Eurasian Patent Office
Prior art keywords
aptamers
ostov
reliable
small molecules
genetically encoded
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EA201991446A
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English (en)
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Роберт Т. Батей
Эли Б. Портер
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Дзе Регентс Оф Дзе Университи Оф Колорадо, А Боди Корпорэйт
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Publication of EA201991446A1 publication Critical patent/EA201991446A1/ru

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Abstract

В данном документе представлены библиотеки остовов, полученных из рибопереключателей и малых рибозимов, и способы их применения. Остовы по данному изобретению дают аптамеры, которые легко идентифицируются и характеризуются благодаря указанному структурному остову. Природа остова предрасполагает эти РНК для соединения со считывающими доменами для конструирования биосенсоров, которые функционируют in vitro и in vivo. Биосенсоры, синтетические РНК-агенты и синтетические ДНК-агенты и способы их применения также обеспечиваются.
EA201991446A 2016-12-12 2017-12-11 Использование биологических рнк-остовов с селекцией in vitro для получения эффективных и надежных аптамеров, связывающих малые молекулы, для генетически кодируемых биосенсоров EA201991446A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662432879P 2016-12-12 2016-12-12
PCT/US2017/065526 WO2018111745A1 (en) 2016-12-12 2017-12-11 Use of biological rna scaffolds with in vitro selection to generate robust small molecule binding aptamers for genetically encodable biosensors

Publications (1)

Publication Number Publication Date
EA201991446A1 true EA201991446A1 (ru) 2020-03-03

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US (2) US20200208142A1 (ru)
EP (1) EP3551755A4 (ru)
JP (2) JP7418210B2 (ru)
KR (1) KR102612226B1 (ru)
CN (1) CN110462039A (ru)
AU (1) AU2017378078B2 (ru)
BR (1) BR112019012012A2 (ru)
CA (1) CA3056218A1 (ru)
EA (1) EA201991446A1 (ru)
IL (1) IL267216A (ru)
MX (1) MX2019006825A (ru)
PH (1) PH12019501302A1 (ru)
WO (1) WO2018111745A1 (ru)

Families Citing this family (9)

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WO2018165566A1 (en) 2017-03-09 2018-09-13 Auburn University Differential circuit for background correction in electrochemical measurements
WO2019010341A1 (en) 2017-07-07 2019-01-10 Innamed, Inc. APTAMERS FOR MEASURING LIPOPROTEIN RATES
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
US20230333128A1 (en) * 2019-06-27 2023-10-19 Innamed, Inc. Assay method for point of care quantification of an immunophilin-binding immunosuppressant drug
WO2021256582A1 (ko) * 2020-06-17 2021-12-23 포항공과대학교 산학협력단 전사 활성 인자 및 토홀드 스위치를 포함하는 트립토판 검출용 바이오센서
WO2022006042A1 (en) * 2020-06-29 2022-01-06 President And Fellows Of Harvard College Methods, compositions, and kits for nucleic acid barcoding of biomolecules
CN113512089B (zh) * 2021-06-30 2023-06-13 兰州大学 一种水溶性量子点的多肽稳定剂及应用
CN114518359A (zh) * 2022-03-08 2022-05-20 山东理工大学 一种基于g-四联体的双模式卡那霉素适配体传感器的制备方法
CN116170470B (zh) * 2023-02-28 2023-07-04 北京科易为科技有限公司 移液器系统的数据通讯方法及其通讯系统

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US20030162190A1 (en) * 2001-11-15 2003-08-28 Gorenstein David G. Phosphoromonothioate and phosphorodithioate oligonucleotide aptamer chip for functional proteomics
AU2003245455A1 (en) * 2002-06-13 2003-12-31 Amgen Inc. Hammerhead ribozymes
US20080269258A1 (en) * 2004-11-08 2008-10-30 Breaker Ronald R Riboswitches, Structure-Based Compound Design with Riboswitches, and Methods and Compositions for Use of and with Riboswitches
AU2008266684A1 (en) * 2007-05-29 2008-12-24 Yale University Riboswitches and methods and compositions for use of and with riboswitches
WO2012021554A1 (en) * 2010-08-09 2012-02-16 Yale University Cyclic di-gmp-ii riboswitches, motifs, and compounds, and methods for their use
US10294478B2 (en) * 2011-08-16 2019-05-21 The Research Foundation For The State University Of New York Aptamer modulators of estrogen receptors
CN104293793B (zh) * 2014-07-24 2018-05-18 江南大学 一种特异识别t-2毒素的寡核苷酸适配体

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JP2023093572A (ja) 2023-07-04
EP3551755A4 (en) 2020-06-24
CN110462039A (zh) 2019-11-15
JP2020504096A (ja) 2020-02-06
WO2018111745A1 (en) 2018-06-21
AU2017378078B2 (en) 2024-06-13
AU2017378078A1 (en) 2019-07-25
CA3056218A1 (en) 2018-06-21
KR20190100227A (ko) 2019-08-28
PH12019501302A1 (en) 2019-10-28
KR102612226B1 (ko) 2023-12-12
JP7418210B2 (ja) 2024-01-19
US20220170009A1 (en) 2022-06-02
BR112019012012A2 (pt) 2019-10-29
US20200208142A1 (en) 2020-07-02
EP3551755A1 (en) 2019-10-16
IL267216A (en) 2019-08-29
MX2019006825A (es) 2019-10-30

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