EA201991446A1 - Использование биологических рнк-остовов с селекцией in vitro для получения эффективных и надежных аптамеров, связывающих малые молекулы, для генетически кодируемых биосенсоров - Google Patents
Использование биологических рнк-остовов с селекцией in vitro для получения эффективных и надежных аптамеров, связывающих малые молекулы, для генетически кодируемых биосенсоровInfo
- Publication number
- EA201991446A1 EA201991446A1 EA201991446A EA201991446A EA201991446A1 EA 201991446 A1 EA201991446 A1 EA 201991446A1 EA 201991446 A EA201991446 A EA 201991446A EA 201991446 A EA201991446 A EA 201991446A EA 201991446 A1 EA201991446 A1 EA 201991446A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- aptamers
- ostov
- reliable
- small molecules
- genetically encoded
- Prior art date
Links
- 108091023037 Aptamer Proteins 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 title abstract 2
- 150000003384 small molecules Chemical class 0.000 title 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 1
- 102000053642 Catalytic RNA Human genes 0.000 abstract 1
- 108090000994 Catalytic RNA Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108020004422 Riboswitch Proteins 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 108091092562 ribozyme Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
- C12N2330/31—Libraries, arrays
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662432879P | 2016-12-12 | 2016-12-12 | |
| PCT/US2017/065526 WO2018111745A1 (en) | 2016-12-12 | 2017-12-11 | Use of biological rna scaffolds with in vitro selection to generate robust small molecule binding aptamers for genetically encodable biosensors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA201991446A1 true EA201991446A1 (ru) | 2020-03-03 |
Family
ID=62559449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201991446A EA201991446A1 (ru) | 2016-12-12 | 2017-12-11 | Использование биологических рнк-остовов с селекцией in vitro для получения эффективных и надежных аптамеров, связывающих малые молекулы, для генетически кодируемых биосенсоров |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US20200208142A1 (enExample) |
| EP (1) | EP3551755A4 (enExample) |
| JP (2) | JP7418210B2 (enExample) |
| KR (1) | KR102612226B1 (enExample) |
| CN (1) | CN110462039A (enExample) |
| AU (1) | AU2017378078B2 (enExample) |
| BR (1) | BR112019012012A2 (enExample) |
| CA (1) | CA3056218A1 (enExample) |
| EA (1) | EA201991446A1 (enExample) |
| IL (1) | IL267216B2 (enExample) |
| MX (1) | MX2019006825A (enExample) |
| MY (1) | MY205553A (enExample) |
| PH (1) | PH12019501302A1 (enExample) |
| WO (1) | WO2018111745A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10852274B2 (en) | 2017-03-09 | 2020-12-01 | Auburn University | Differential circuit for background correction in electrochemical measurements |
| US11505799B2 (en) | 2017-07-07 | 2022-11-22 | Innamed, Inc. | Aptamers for measuring lipoprotein levels |
| US11560565B2 (en) | 2018-06-13 | 2023-01-24 | Auburn University | Electrochemical detection nanostructure, systems, and uses thereof |
| WO2020264444A2 (en) * | 2019-06-27 | 2020-12-30 | Innamed, Inc. | Assay method for point of care quantification of an immunophilin-binding immunosuppressant drug |
| CN113430203A (zh) * | 2020-03-23 | 2021-09-24 | 华东理工大学 | 一种新型rna检测与定量的方法 |
| WO2021256582A1 (ko) * | 2020-06-17 | 2021-12-23 | 포항공과대학교 산학협력단 | 전사 활성 인자 및 토홀드 스위치를 포함하는 트립토판 검출용 바이오센서 |
| WO2022006042A1 (en) * | 2020-06-29 | 2022-01-06 | President And Fellows Of Harvard College | Methods, compositions, and kits for nucleic acid barcoding of biomolecules |
| CN115177740A (zh) * | 2021-04-01 | 2022-10-14 | 安升（上海）医药科技有限公司 | 核酸自组装介导的adc药物的构建方法及应用 |
| CN113512089B (zh) * | 2021-06-30 | 2023-06-13 | 兰州大学 | 一种水溶性量子点的多肽稳定剂及应用 |
| CN115704029A (zh) * | 2021-08-06 | 2023-02-17 | 纳莹(上海)生物科技有限公司 | 一种rna检测与定量的方法 |
| CN114518359B (zh) * | 2022-03-08 | 2024-08-20 | 山东理工大学 | 一种基于g-四联体的双模式卡那霉素适配体传感器的制备方法 |
| CN115791726B (zh) * | 2022-11-25 | 2024-11-15 | 海南大学 | 一种基于g-四联体dna超灵敏高选择性检测锶离子的方法 |
| WO2024118806A1 (en) * | 2022-11-29 | 2024-06-06 | The Johns Hopkins University | Aptamer regulated transcription for in-vitro sensing and transduction |
| CN116170470B (zh) * | 2023-02-28 | 2023-07-04 | 北京科易为科技有限公司 | 移液器系统的数据通讯方法及其通讯系统 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060121510A1 (en) * | 1998-11-03 | 2006-06-08 | Breaker Ronald R | Multidomain polynucleotide molecular sensors |
| US20030162190A1 (en) * | 2001-11-15 | 2003-08-28 | Gorenstein David G. | Phosphoromonothioate and phosphorodithioate oligonucleotide aptamer chip for functional proteomics |
| US20060121466A1 (en) * | 2002-06-13 | 2006-06-08 | Amgen Inc. | Hammerhead ribozymes |
| US20050019916A1 (en) * | 2003-04-14 | 2005-01-27 | Stojanovic Milan N. | Cross reactive arrays of three-way junction sensors for steriod determination |
| MX2007005491A (es) * | 2004-11-08 | 2007-09-27 | Univ Yale | Diseno de compuesto basado en la estructura que involucra riboconmutadores. |
| SG174103A1 (en) * | 2007-05-29 | 2011-09-29 | Univ Yale | Riboswitches and methods and compositions for use of and with riboswitches |
| WO2012021554A1 (en) * | 2010-08-09 | 2012-02-16 | Yale University | Cyclic di-gmp-ii riboswitches, motifs, and compounds, and methods for their use |
| US8383794B2 (en) * | 2010-10-08 | 2013-02-26 | Li Niu | Conformation-selective nucleic acid inhibitors of AMPA glutamate receptors |
| US20120248666A1 (en) * | 2011-03-30 | 2012-10-04 | Firestone Industrial Products Company, Llc | Gas spring and damper assembly and suspension system including same |
| US10294478B2 (en) * | 2011-08-16 | 2019-05-21 | The Research Foundation For The State University Of New York | Aptamer modulators of estrogen receptors |
| US9664676B2 (en) * | 2013-09-06 | 2017-05-30 | Cornell University | RNA sequences that induce fluorescence of small molecule fluorophores |
| CN104293793B (zh) * | 2014-07-24 | 2018-05-18 | 江南大学 | 一种特异识别t-2毒素的寡核苷酸适配体 |
-
2017
- 2017-12-11 WO PCT/US2017/065526 patent/WO2018111745A1/en not_active Ceased
- 2017-12-11 CA CA3056218A patent/CA3056218A1/en active Pending
- 2017-12-11 EP EP17880409.2A patent/EP3551755A4/en active Pending
- 2017-12-11 IL IL267216A patent/IL267216B2/en unknown
- 2017-12-11 CN CN201780085607.6A patent/CN110462039A/zh active Pending
- 2017-12-11 BR BR112019012012-2A patent/BR112019012012A2/pt active Search and Examination
- 2017-12-11 KR KR1020197019421A patent/KR102612226B1/ko active Active
- 2017-12-11 AU AU2017378078A patent/AU2017378078B2/en active Active
- 2017-12-11 MX MX2019006825A patent/MX2019006825A/es unknown
- 2017-12-11 JP JP2019531196A patent/JP7418210B2/ja active Active
- 2017-12-11 MY MYPI2019003292A patent/MY205553A/en unknown
- 2017-12-11 EA EA201991446A patent/EA201991446A1/ru unknown
- 2017-12-11 US US16/468,945 patent/US20200208142A1/en not_active Abandoned
-
2019
- 2019-06-10 PH PH12019501302A patent/PH12019501302A1/en unknown
-
2021
- 2021-12-02 US US17/541,068 patent/US20220170009A1/en active Pending
-
2023
- 2023-04-11 JP JP2023064371A patent/JP2023093572A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3551755A4 (en) | 2020-06-24 |
| JP2023093572A (ja) | 2023-07-04 |
| JP2020504096A (ja) | 2020-02-06 |
| MY205553A (en) | 2024-10-25 |
| IL267216B2 (en) | 2025-04-01 |
| US20200208142A1 (en) | 2020-07-02 |
| AU2017378078B2 (en) | 2024-06-13 |
| EP3551755A1 (en) | 2019-10-16 |
| KR102612226B1 (ko) | 2023-12-12 |
| PH12019501302A1 (en) | 2019-10-28 |
| JP7418210B2 (ja) | 2024-01-19 |
| CN110462039A (zh) | 2019-11-15 |
| BR112019012012A2 (pt) | 2019-10-29 |
| MX2019006825A (es) | 2019-10-30 |
| WO2018111745A1 (en) | 2018-06-21 |
| AU2017378078A1 (en) | 2019-07-25 |
| KR20190100227A (ko) | 2019-08-28 |
| IL267216A (en) | 2019-08-29 |
| CA3056218A1 (en) | 2018-06-21 |
| IL267216B1 (en) | 2024-12-01 |
| US20220170009A1 (en) | 2022-06-02 |
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