JP7391971B2 - 新規のｄｎａアプタマー及びこれの用途 - Google Patents

新規のｄｎａアプタマー及びこれの用途 Download PDF

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JP7391971B2
JP7391971B2 JP2021536744A JP2021536744A JP7391971B2 JP 7391971 B2 JP7391971 B2 JP 7391971B2 JP 2021536744 A JP2021536744 A JP 2021536744A JP 2021536744 A JP2021536744 A JP 2021536744A JP 7391971 B2 JP7391971 B2 JP 7391971B2
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aptamer
cancer
present disclosure
dna aptamer
cells
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JP2022516054A (ja
JPWO2020130269A5 (https=
JP2022516054A5 (https=
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キム，ユンヒ
ホ，ギュン
チェ，ソンイル
キム，インフ
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Jp Bio A Inc
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Jp Bio A Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021536744A 2018-12-21 2019-08-08 新規のｄｎａアプタマー及びこれの用途 Active JP7391971B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20180167948 2018-12-21
KR10-2018-0167948 2018-12-21
PCT/KR2019/009956 WO2020130269A1 (ko) 2018-12-21 2019-08-08 신규한 dna 앱타머 및 이의 용도

Publications (4)

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JP2022516054A JP2022516054A (ja) 2022-02-24
JPWO2020130269A5 JPWO2020130269A5 (https=) 2022-07-14
JP2022516054A5 JP2022516054A5 (https=) 2022-07-14
JP7391971B2 true JP7391971B2 (ja) 2023-12-05

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JP2021536744A Active JP7391971B2 (ja) 2018-12-21 2019-08-08 新規のｄｎａアプタマー及びこれの用途

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US (1) US12398395B2 (https=)
EP (1) EP3901287A4 (https=)
JP (1) JP7391971B2 (https=)
KR (1) KR102324242B1 (https=)
CN (1) CN113646442B (https=)
WO (2) WO2020130225A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020130225A1 (ko) * 2018-12-21 2020-06-25 국립암센터 췌장암 특이적으로 결합하는 dna 앱타머 및 이의 용도
KR102506295B1 (ko) 2020-08-28 2023-03-08 국립암센터 디그옥시제닌에 대한 인간화 항체 및 이의 용도
KR102506288B1 (ko) * 2020-09-07 2023-03-06 국립암센터 디그옥시제닌에 대한 항체를 포함하는 복합체, 및 이들의 용도

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012528587A (ja) 2009-06-01 2012-11-15 ソンギュングヮン ユニバーシティ ファウンデーション フォー コーポレート コラボレーション 膵臓癌細胞または組織に特異的に結合できる核酸アプタマー及びその用途
WO2017073535A1 (ja) 2015-10-30 2017-05-04 タグシクス・バイオ株式会社 癌細胞に結合するｄｎａアプタマー

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
KR101250557B1 (ko) * 2011-05-18 2013-04-03 국립암센터 Ｐａｕｆ 특이적 앱타머 및 이를 포함하는 췌장암 치료용 조성물
KR101699105B1 (ko) * 2012-04-02 2017-01-23 성균관대학교산학협력단 Ａｌｐｐｌ２ 단백질을 이용한 췌장암 진단 방법
KR101568400B1 (ko) * 2012-08-09 2015-11-12 연세대학교 산학협력단 암 줄기세포에 특이적으로 결합하는 핵산 앱타머 및 이의 용도
KR101458947B1 (ko) * 2013-03-05 2014-11-12 국립암센터 인터루킨-８ 앱타머 및 이의 용도
WO2020130225A1 (ko) * 2018-12-21 2020-06-25 국립암센터 췌장암 특이적으로 결합하는 dna 앱타머 및 이의 용도
KR102506295B1 (ko) * 2020-08-28 2023-03-08 국립암센터 디그옥시제닌에 대한 인간화 항체 및 이의 용도
KR102506288B1 (ko) * 2020-09-07 2023-03-06 국립암센터 디그옥시제닌에 대한 항체를 포함하는 복합체, 및 이들의 용도

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012528587A (ja) 2009-06-01 2012-11-15 ソンギュングヮン ユニバーシティ ファウンデーション フォー コーポレート コラボレーション 膵臓癌細胞または組織に特異的に結合できる核酸アプタマー及びその用途
WO2017073535A1 (ja) 2015-10-30 2017-05-04 タグシクス・バイオ株式会社 癌細胞に結合するｄｎａアプタマー

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHAMPANHAC, C. et al.，Development of a panel of DNA aptamers with high affinity for pancreatic ductal adenocarcinoma，Scientific Reports，2015年，vol. 5，16788
CLAWSON, G. A. et al.，A cholecystokinin B receptor-specific DNA aptamer for targeting pancreatic ductal adenocarcinoma，Nucleic Acid Therapeutics，2017年，vol.27, no.1，p.23-35
WU, X. et al.，DNA aptamer selected against pancreatic ductal adenocarcinoma for in vivo imaging and clinical tissue recognition，Theranostics，2015年，vol.5, no.9，p.985-994

Also Published As

Publication number Publication date
US20220090081A1 (en) 2022-03-24
WO2020130269A1 (ko) 2020-06-25
US12398395B2 (en) 2025-08-26
JP2022516054A (ja) 2022-02-24
CN113646442A (zh) 2021-11-12
EP3901287A4 (en) 2022-10-26
KR20200078303A (ko) 2020-07-01
WO2020130225A1 (ko) 2020-06-25
KR102324242B1 (ko) 2021-11-11
CN113646442B (zh) 2024-12-03
EP3901287A1 (en) 2021-10-27

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