JP7181880B2 - 免疫療法のためのコア/シェル構造プラットホーム - Google Patents
免疫療法のためのコア/シェル構造プラットホーム Download PDFInfo
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- JP7181880B2 JP7181880B2 JP2019540384A JP2019540384A JP7181880B2 JP 7181880 B2 JP7181880 B2 JP 7181880B2 JP 2019540384 A JP2019540384 A JP 2019540384A JP 2019540384 A JP2019540384 A JP 2019540384A JP 7181880 B2 JP7181880 B2 JP 7181880B2
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Description
本出願は、PCT国際特許出願PCT/US2018/015601(係属中;同時に出願;Atty.Dkt 37182.215WO01)および、2017年1月27に出願された米国仮特許出願62/451,575(係属中;Atty.Dkt 37182.215PV01)に対する優先権を主張し、その内容は、その全体が本明細書に、明白な参照により具体的に援用される。
本発明は、アメリカ国防総省により与えられたW81XWH-12-1-0414の助成金ならびに、アメリカ国立衛生研究所により与えられた1R01-CA193880-01A1およびU43-CA210181の助成金の下、政府支援によりなされた。米国政府は、本発明における一定の権利を有する。
ワクチンは通常、身体の免疫系によって認識され得る抗原および身体の免疫反応を増強し得る1または複数のアジュバントから構成されている。しかし治療用がんワクチンは感染性疾患を防止するために使用される予防用ワクチンとは全く異なっている。それは、ワクチンは病原体と戦う抗体を産生する代わりに、がん細胞を殺滅する免疫細胞を生成するのに十分に強力でなければならないからである。治療用ワクチンは、がんおよび感染性疾患を含む複数の種類の生命を脅かす疾患の処置において大きな潜在性を有している。がんワクチン接種の成功を決定する重要な課題は、選択した抗原に対する抗腫瘍応答の強力な誘導である。タンパク質ペプチドおよびDNAプラスミドの両方は、従来よりワクチン開発のための抗原として役立ってきた(Meleroら、2014年;Schwartzentruberら、2011年;Kantoffら、2010年)。タンパク質およびペプチドは調製が比較的容易で、大スケールで産生することができるが、抗原ペプチドの選択は患者の独特の主要組織適合性複合体(MHC)タンパク質の型に依存し、したがって個別の患者に適合するようにカスタム化する必要がある。一方、DNAワクチンは効力が低いという欠点があり、制御できないゲノム組込みのリスクがある(McNamaraら、2015年)。
(a)約30%~約70%の1,2-ジオレオイル-sn-グリセロ-3-エチルホスホコリン(「EDOPC」)、
(b)約70%~約30%の1,2-ジオレオイル-sn-グリセロ-3-ホスファチジル-エタノールアミン(「DOPE」)、または
(c)約0.5~約5%の1,2-ジステアロイル-sn-グリセロ-3-ホスホエタノールアミン-N-[アミノ(ポリエチレングリコール)-2000](「DSPE-PEG」)
を含む。
(a)約45%~約55%のEDOPC、
(b)約55%~約45%のDOPE、および
(c)約1%~約2%のDSPE-PEG
を、含んでよい。
配列番号1は、野生型TP53ペプチド由来の例示的な細胞傷害性T細胞エピトープであり、本開示の1または複数の態様に従って有用である。
説明的実施形態の記述
がん
mRNAに基づく治療用がんワクチン
mRNAをパッケージするためのリポポリプレックス組成物
医薬製剤
医薬の調製のための組成物
例示的定義
生物学的機能等価
(実施例1)
リポポリプレックスはmRNAに基づくワクチンの抗腫瘍免疫を増強する
材料および方法
結果
(実施例2)
リポポリプレックスはmRNAに基づくワクチンの抗腫瘍免疫を増強する
(実施例3)
mRNAワクチンプラットフォームの一般化した説明
(実施例4)
2つのmRNAを、デュアル免疫療法のために単一シェル中に共パッケージ化することができる
(実施例5)
個別化がん免疫療法のためのmRNAに基づくワクチン
a)より効果的で毒性が低く、生存期間に影響を与える処置レジメンに置き換えることにより、処置レジメンに革命をもたらすこと;
b)転移性乳がんに関連する死亡率を排除すること;および
c)乳がん細胞が何年も休眠状態であり、その後再出現する理由と方法を解明し、この情報を使用してそのような再発を防止すること。
参考文献
本発明は、例えば、以下の項目を提供する。
(項目1)
少なくとも第1の腫瘍抗原をコードするmRNA分子の集団を含む治療用がんワクチンを含む組成物であって、該集団が少なくとも第1の正電荷ポリマーまたはタンパク質を含む複数のポリプレックスまたはタンパク質コア粒子の中に含まれ、さらに該複数のポリプレックスまたはタンパク質コア粒子がそれ自体、第1の生体適合性脂質二重層シェルにカプセル化されている、組成物。
(項目2)
前記第1の生体適合性脂質二重層シェルが、1または複数の哺乳動物の抗原提示細胞による前記複数のポリプレックスまたはタンパク質コア粒子のマクロピノサイトーシスを促進する、項目1に記載の組成物。
(項目3)
前記生体適合性脂質二重層の中にカプセル化された、CpG、ポリ(I:C)、アラム、およびそれらの任意の組合せからなる群から選択されるアジュバントをさらに含む、項目1または項目2に記載の組成物。
(項目4)
前記生体適合性脂質二重層の間の空間の中にカプセル化された、IL-12p70タンパク質、FLT3リガンド、またはインドールアミン2,3-ジオキシゲナーゼ(IDO-1)阻害剤等の免疫調節化合物をさらに含む、先行する項目のいずれかに記載の組成物。
(項目5)
前記インドールアミン2,3-ジオキシゲナーゼ(IDO-1)阻害剤が、GDC-0919、INCB24360、またはそれらの組合せである、項目4に記載の組成物。
(項目6)
前記正電荷ポリマーまたはタンパク質が、プロタミン、ポリエチレンイミン、ポリ-(B-アミノエステル)、またはそれらの任意の組合せを含む、先行する項目のいずれかに記載の組成物。
(項目7)
前記生体適合性脂質二重層が、1,2-ジオレオイル-sn-グリセロ-3-エチルホスホコリン(EDOPC)、1,2-ジオレオイル-sn-グリセロ-3-ホスファチジル-エタノールアミン(DOPE)、1,2-ジステアロイル-sn-グリセロ-3-ホスホエタノールアミン-N-[アミノ(ポリエチレングリコール)-2000](DSPE-PEG)、およびそれらの組合せの1または複数を含む、先行する項目のいずれかに記載の組成物。
(項目8)
前記生体適合性脂質二重層が、
(a)約30%~約70%の1,2-ジオレオイル-sn-グリセロ-3-エチルホスホコリン(EDOPC)、
(b)約70%~約30%の1,2-ジオレオイル-sn-グリセロ-3-ホスファチジル-エタノールアミン(DOPE)、または
(c)約0.5~約5%の1,2-ジステアロイル-sn-グリセロ-3-ホスホエタノールアミン-N-[アミノ(ポリエチレングリコール)-2000](DSPE-PEG)
を含む、先行する項目のいずれかに記載の組成物。
(項目9)
前記生体適合性脂質二重層が、
(a)約45%~約55%の1,2-ジオレオイル-sn-グリセロ-3-エチルホスホコリン(EDOPC)、
(b)約55%~約45%の1,2-ジオレオイル-sn-グリセロ-3-ホスファチジルエタノールアミン(DOPE)、および(c)約1~約2%の1,2-ジステアロイル-sn-グリセロ-3-ホスホエタノールアミン-N-[アミノ(ポリエチレングリコール)-2000](DSPE-PEG)
を含む、先行する項目のいずれかに記載の組成物。
(項目10)
mRNA分子の前記集団が、哺乳動物のがん細胞に特異的な少なくとも1つの抗原をコードする、先行する項目のいずれかに記載の組成物。
(項目11)
mRNA分子の前記集団が、少なくとも1つのがんもしくは腫瘍特異的タンパク質、ポリペプチドもしくはペプチド、またはその抗原性断片をコードする、先行する項目のいずれかに記載の組成物。
(項目12)
mRNA分子の前記集団が、HER2p66、HER2E75、HER2 YVMA 、TRP2、p66、およびそれらの任意の組合せからなる群から選択される少なくとも1つのヒト腫瘍特異的ポリペプチドまたはペプチドをコードする、先行する項目のいずれかに記載の組成物。
(項目13)
好適な哺乳動物の細胞に導入されたときにI型インターフェロン(IFN-I)の発現を増加させるように、好ましくはIFN-α4、IFN-β、またはそれらの組合せの発現を増加させるように、適合され構成された、先行する項目のいずれかに記載の組成物。
(項目14)
哺乳動物の抗原提示細胞、例えば、ヒト樹状細胞、マクロファージ細胞、B細胞、がん細胞、またはそれらの組合せの集団に導入されたときに、IFN-Iの発現を増加させる、先行する項目のいずれかに記載の組成物。
(項目15)
治療剤、例えば、免疫調節剤、抗新生物剤、細胞傷害性薬剤、細胞増殖抑制剤、神経活性薬剤、抗炎症剤、抗脂血症剤、ホルモン、受容体アゴニスト、受容体アンタゴニスト、抗感染症剤、タンパク質、ペプチド、抗体、抗原結合性断片、酵素、RNA、DNA、siRNA、mRNA、リボザイム、ホルモン、コファクター、ステロイド、アンチセンス分子、またはそれらの任意の組合せからなる群から選択される薬剤をさらに含む、先行する項目のいずれかに記載の組成物。
(項目16)
化学療法剤が、シクロホスファミド、ドキソルビシン、5-フルオロウラシル、ドセタキセル、パクリタキセル、トラスツズマブ、メトトレキセート、エピルビシン、シスプラチン、カルボプラチン、ビノレルビン、カペシタビン、ゲムシタビン、ミトキサントロン、イサベピロン、エリブリン、ラパチニブ、カルムスチン、窒素マスタード、硫黄マスタード、四硝酸プラチン、ビンブラスチン、エトポシド、カンプトテシン、およびそれらの任意の組合せからなる群から選択される化合物を含む、項目13に記載の組成物。
(項目17)
抗原ポリペプチド、抗原性融合ポリペプチド、抗原性ペプチド、またはその断片をさらに含む、先行する項目のいずれかに記載の組成物。
(項目18)
メソ多孔質ケイ素粒子、ナノ粒子、ミクロ粒子、またはそれらの任意の組合せの集団の中に含まれた、先行する項目のいずれかに記載の組成物。
(項目19)
1または複数の界面活性剤、リポソーム、ニオソーム、エトソーム、トランスフェロソーム、リン脂質、スフィンゴソーム、またはそれらの任意の組合せと混合された、先行する項目のいずれかに記載の組成物。
(項目20)
1または複数の薬学的に許容される担体、緩衝剤、希釈剤、ビヒクル、または賦形剤をさらに含む、先行する項目のいずれかに記載の組成物。
(項目21)
哺乳動物への全身投与のため、好ましくはヒトへの皮内または静脈内投与のために製剤化された、先行する項目のいずれかに記載の組成物。
(項目22)
哺乳動物の抗原提示細胞、例えば、がん細胞、腫瘍細胞、マクロファージ細胞、B細胞、樹状細胞、またはそれらの任意の組合せの単離された集団の中に含まれた、先行する項目のいずれかに記載の組成物。
(項目23)
前記組成物および該組成物の投与を必要とするヒトへの該組成物の投与のための少なくとも第1のセットの指示を含む治療用キットの一部として適合され構成された、先行する項目のいずれかに記載の組成物。
(項目24)
哺乳動物のがんの1または複数の症状の予防、治療、または改善における使用のための、先行する項目のいずれかに記載の組成物。
(項目25)
ヒトのがんの1または複数の症状の治療または改善における使用のための、先行する項目のいずれかに記載の組成物。
(項目26)
ヒトの転移がんの1または複数の症状の治療または改善における使用のための、先行する項目のいずれかに記載の組成物。
(項目27)
先行する項目のいずれかに記載の組成物を含む、哺乳動物細胞の単離された集団。
(項目28)
抗原提示細胞、好ましくは、ヒト樹状細胞、マクロファージ、B細胞、またはそれらの組合せとして特徴付けられる、項目27に記載の哺乳動物細胞の単離された集団。
(項目29)
哺乳動物対象における疾患、障害、または機能不全、例えばがんの少なくとも1つの症状を処置しまたは改善するための医薬の製造における、項目1から26のいずれか一項に記載の組成物の使用。
(項目30)
前記哺乳動物対象がヒト、非ヒト霊長類、伴侶動物、外来種、家畜、または原料動物である、項目29に記載の使用。
(項目31)
1)項目1から26のいずれか一項に記載の組成物、および2)哺乳動物における疾患、障害、異常な状態、または機能不全、例えば、がんまたは過剰増殖性障害の1または複数の症状の防止、処置、または改善のためのレジメンの一部としての、該組成物の投与を必要とする該哺乳動物への該組成物の投与のための指示、を含むキット。
(項目32)
がんの1または複数の症状の処置または改善を必要とする哺乳動物におけるがんの1または複数の症状を処置しまたは改善する方法であって、該哺乳動物における疾患、障害、異常な状態、または機能不全の1または複数の症状を処置しまたは改善するのに十分な時間、有効量の項目1から26のいずれか一項に記載の組成物を該動物に投与するステップを含む、方法。
(項目33)
前記疾患が、難治性、転移、再発、または治療抵抗性がんとして診断されまたは同定されている、項目32に記載の方法。
(項目34)
前記がんが、転移乳がん、転移肺がん、または転移黒色腫である、項目33に記載の方法。
(項目35)
前記哺乳動物がヒトである、項目32から34のいずれか一項に記載の方法。
(項目36)
前記哺乳動物に治療有効量の放射線または追加の化学療法剤を投与するステップをさらに含む、項目32から35のいずれか一項に記載の方法。
(項目37)
前記組成物が、単回投与で、あるいは1日もしくはそれより長い日数からの期間にわたる、1週もしくはそれより長い週数の期間にわたる、または1か月もしくはそれより長い月数の期間にわたる、またはそれより長い期間にわたる一連の複数回投与で、前記哺乳動物に全身投与される、項目32から36のいずれか一項に記載の方法。
(項目38)
前記組成物が、化学療法剤、または第2の異なる治療用がんワクチンをさらに含む、項目32から37のいずれか一項に記載の方法。
(項目39)
治療用抗がん抗原をコードするmRNAの投与を必要とする哺乳動物対象の体内のがん細胞の集団に、治療用抗がん抗原をコードするmRNAを投与する方法であって、有効量の項目1から26のいずれか一項に記載の組成物を該対象に投与するステップを含む、方法。
Claims (38)
- 抗原をコードするmRNA分子の集団を含む組成物であって、mRNA分子の該集団が少なくともポリマーまたはタンパク質を含む複数のポリプレックスまたはタンパク質コア粒子の中に含まれ、該複数のポリプレックスまたはタンパク質コア粒子がそれ自体、生体適合性脂質二重層シェルにカプセル化されており、
該生体適合性脂質二重層シェルが、
(a)約45%~約55%の1,2-ジオレオイル-sn-グリセロ-3-エチルホスホコリン(EDOPC)、
(b)約55%~約45%の1,2-ジオレオイル-sn-グリセロ-3-ホスファチジルエタノールアミン(DOPE)、および
(c)約1%~約2%の1,2-ジステアロイル-sn-グリセロ-3-ホスホエタノールアミン-N-[アミノ(ポリエチレングリコール)-2000](DSPE-PEG)を含む、組成物。 - 前記生体適合性脂質二重層シェルが、1または複数の哺乳動物の抗原提示細胞による前記複数のポリプレックスまたはタンパク質コア粒子のマクロピノサイトーシスを促進する、請求項1に記載の組成物。
- 前記生体適合性脂質二重層シェルの中にカプセル化された、CpG、ポリ(I:C)、アラム、およびそれらの任意の組合せからなる群から選択されるアジュバントをさらに含む、請求項1または請求項2に記載の組成物。
- IL-12p70タンパク質、FLT3リガンド、およびインドールアミン2,3-ジオキシゲナーゼ(IDO-1)阻害剤からなる群より選択される免疫調節化合物をさらに含み、該免疫調節化合物は、前記生体適合性脂質二重層シェルの間の空間の中にカプセル化されており、該インドールアミン2,3-ジオキシゲナーゼ(IDO-1)阻害剤が、GDC-0919、INCB24360、またはそれらの組合せである、請求項1~3のいずれかに記載の組成物。
- 前記ポリマーまたはタンパク質が、プロタミン、ポリエチレンイミン、ポリ-(B-アミノエステル)、またはそれらの任意の組合せを含む、請求項1~4のいずれかに記載の組成物。
- 前記抗原が哺乳動物の細胞に特異的である、請求項1~5のいずれかに記載の組成物。
- mRNA分子の前記集団が、少なくとも1つのがんもしくは腫瘍特異的タンパク質、ポリペプチドもしくはペプチド、またはその抗原性断片をコードする、請求項1~6のいずれかに記載の組成物。
- mRNA分子の前記集団が、HER2p66、HER2E75、HER2YVMA、TRP2、p66、およびそれらの任意の組合せからなる群から選択される少なくとも1つのヒト腫瘍特異的ポリペプチドまたはペプチドをコードする、請求項1~7のいずれかに記載の組成物。
- 好適な哺乳動物の細胞に導入されたときにI型インターフェロン(IFN-I)の発現を増加させるように、好ましくはIFN-α4、IFN-β、またはそれらの組合せの発現を増加させるように、適合され構成された、請求項1~8のいずれかに記載の組成物。
- ヒト樹状細胞、マクロファージ細胞、B細胞、がん細胞、またはそれらの組合せを含む哺乳動物の抗原提示細胞の集団に導入されたときに、IFN-Iの発現を増加させる、請求項1~9のいずれかに記載の組成物。
- 免疫調節剤、抗新生物剤、細胞傷害性薬剤、細胞増殖抑制剤、神経活性薬剤、抗炎症剤、抗脂血症剤、ホルモン、受容体アゴニスト、受容体アンタゴニスト、抗感染症剤、タンパク質、ペプチド、抗体、抗原結合性断片、酵素、RNA、DNA、siRNA、mRNA、リボザイム、ホルモン、コファクター、ステロイド、アンチセンス分子、およびそれらの任意の組合せからなる群から選択される治療剤をさらに含む、請求項1~10のいずれかに記載の組成物。
- 前記治療剤が、シクロホスファミド、ドキソルビシン、5-フルオロウラシル、ドセタキセル、パクリタキセル、トラスツズマブ、メトトレキセート、エピルビシン、シスプラチン、カルボプラチン、ビノレルビン、カペシタビン、ゲムシタビン、ミトキサントロン、イサベピロン、エリブリン、ラパチニブ、カルムスチン、窒素マスタード、硫黄マスタード、四硝酸プラチン、ビンブラスチン、エトポシド、カンプトテシン、およびそれらの任意の組合せからなる群から選択される、請求項11に記載の組成物。
- 抗原ポリペプチド、抗原性融合ポリペプチド、抗原性ペプチド、またはその断片をさらに含む、請求項1~12のいずれかに記載の組成物。
- メソ多孔質ケイ素粒子、ナノ粒子、ミクロ粒子、またはそれらの任意の組合せの集団の中に含まれた、請求項1~13のいずれかに記載の組成物。
- 1または複数の界面活性剤、リポソーム、ニオソーム、エトソーム、トランスフェロソーム、リン脂質、スフィンゴソーム、またはそれらの任意の組合せをさらに含む、請求項1~14のいずれかに記載の組成物。
- 1または複数の薬学的に許容される担体、緩衝剤、希釈剤、ビヒクル、または賦形剤をさらに含む、請求項1~15のいずれかに記載の組成物。
- 哺乳動物への全身投与のため、好ましくはヒトへの皮内または静脈内投与のために製剤化された、請求項1~16のいずれかに記載の組成物。
- がん細胞、腫瘍細胞、マクロファージ細胞、B細胞、樹状細胞、またはそれらの任意の組合せを含む哺乳動物の抗原提示細胞の単離された集団の中に含まれた、請求項1~17のいずれかに記載の組成物。
- 前記組成物および該組成物の投与を必要とするヒトへの該組成物の投与のための少なくともセットの指示を含む治療用キットの一部として適合され構成された、請求項1~18のいずれかに記載の組成物。
- 哺乳動物のがんの1または複数の症状の予防、治療、または改善における使用のための、請求項1~19のいずれかに記載の組成物。
- ヒトのがんの1または複数の症状の治療または改善における使用のための、請求項1~20のいずれかに記載の組成物。
- ヒトの転移がんの1または複数の症状の治療または改善における使用のための、請求項1~21のいずれかに記載の組成物。
- 前記ポリマーまたはタンパク質は正に荷電している、請求項1~22のいずれか一項に記載の組成物。
- 請求項1~23のいずれかに記載の組成物を含む、哺乳動物細胞の単離された集団。
- 前記哺乳動物細胞は、抗原提示細胞、好ましくは、ヒト樹状細胞、マクロファージ、B細胞、またはそれらの組合せからなる群から選択される、請求項24に記載の哺乳動物細胞の単離された集団。
- 哺乳動物対象における疾患、障害、または機能不全の少なくとも1つの症状を処置しまたは改善するための医薬の製造における、請求項1から23のいずれか一項に記載の組成物の使用。
- 前記哺乳動物対象がヒト、非ヒト霊長類、伴侶動物、外来種、家畜、または原料動物である、請求項26に記載の使用。
- 1)請求項1から23のいずれか一項に記載の組成物、および2)哺乳動物における疾患、障害、異常な状態、または機能不全の1または複数の症状の防止、処置、または改善のためのレジメンの一部としての、該組成物の投与を必要とする該哺乳動物への該組成物の投与のための指示、を含むキット。
- 疾患、障害、異常な状態、または機能不全の1または複数の症状の処置または改善を必要とする哺乳動物における疾患、障害、異常な状態、または機能不全の1または複数の症状を処置しまたは改善するための、請求項1から23のいずれか一項に記載の組成物であって、前記組成物が、該哺乳動物における疾患、障害、異常な状態、または機能不全の1または複数の症状を処置しまたは改善するのに十分な時間投与されることを特徴とする、組成物。
- 前記疾患が、難治性、転移、再発、または治療抵抗性がんとして診断されまたは同定されている、請求項29に記載の組成物。
- 前記がんが、転移乳がん、転移肺がん、または転移黒色腫である、請求項30に記載の組成物。
- 前記哺乳動物がヒトである、請求項29から31のいずれか一項に記載の組成物。
- 前記組成物が、前記哺乳動物に放射線または追加の化学療法剤と組み合わせて投与されることを特徴とする、請求項29から32のいずれか一項に記載の組成物。
- 前記組成物が、単回投与で、あるいは1日もしくはそれより長い日数からの期間にわたる、1週もしくはそれより長い週数の期間にわたる、または1か月もしくはそれより長い月数の期間にわたる、またはそれより長い期間にわたる一連の複数回投与で、前記哺乳動物に全身投与される、請求項29から33のいずれか一項に記載の組成物。
- 前記組成物が、化学療法剤、または治療用がんワクチンをさらに含む、請求項29から34のいずれか一項に記載の組成物。
- 抗原をコードするmRNAの投与を必要とする哺乳動物対象の体内の細胞の集団に、抗原をコードするmRNAを投与するための、請求項1から23のいずれか一項に記載の組成物。
- 哺乳動物対象における疾患、障害、または機能不全の少なくとも1つの症状を処置しまたは改善するための、請求項1から23のいずれか一項に記載の組成物。
- 前記哺乳動物対象がヒト、非ヒト霊長類、伴侶動物、外来種、家畜、または原料動物である、請求項37に記載の組成物。
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US11433143B2 (en) | 2017-05-18 | 2022-09-06 | The Regents Of The University Of California | Nano-enabled immunotherapy in cancer |
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JP2022506839A (ja) * | 2018-11-07 | 2022-01-17 | モデルナティエックス インコーポレイテッド | Rnaがんワクチン |
CN109260157A (zh) * | 2018-11-19 | 2019-01-25 | 上海交通大学 | 一种正电荷多肽脂质体纳米制剂及其制备方法与应用 |
CA3133175A1 (en) * | 2019-03-14 | 2020-09-17 | Engene, Inc. | Reversible coating of chitosan-nucleic acid nanoparticles and methods of their use |
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DE102019122014A1 (de) * | 2019-08-15 | 2021-02-18 | Technische Universität Darmstadt | Reduktion der Knochenresorption, insbesondere bei chronischen Gelenkerkrankungen |
WO2021081043A1 (en) * | 2019-10-22 | 2021-04-29 | The Johns Hopkins University | Mucus penetrating particle compositions and methods of use thereof enhancing immune response |
CN112245575A (zh) * | 2020-10-26 | 2021-01-22 | 上海杏禾医疗科技有限公司 | 含支化聚合物和mRNA的核壳结构的抗肿瘤疫苗及其用途 |
WO2023034957A1 (en) * | 2021-09-03 | 2023-03-09 | University Of Connecticut | Stabilization of antigens for long term administration in transdermal microneedle patches |
CN114699420A (zh) * | 2022-03-08 | 2022-07-05 | 南方科技大学 | 一种用于治疗前列腺癌组合物及其制备方法与应用 |
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CN103550783A (zh) * | 2013-04-27 | 2014-02-05 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 一种核酸类药物靶向递送系统及其制备方法 |
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JP2023015346A (ja) | 2023-01-31 |
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US11382964B2 (en) | 2022-07-12 |
KR20240014586A (ko) | 2024-02-01 |
CN115845040A (zh) | 2023-03-28 |
US20220305103A1 (en) | 2022-09-29 |
KR102627347B1 (ko) | 2024-01-22 |
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CN109152830B (zh) | 2023-11-03 |
AU2018212887A1 (en) | 2019-08-08 |
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