JP7177913B2 - 高消光係数標識子と誘電体基板を用いた高感度バイオセンサチップ、測定システム、及び測定方法 - Google Patents
高消光係数標識子と誘電体基板を用いた高感度バイオセンサチップ、測定システム、及び測定方法 Download PDFInfo
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- G—PHYSICS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
- G01N21/211—Ellipsometry
- G01N2021/215—Brewster incidence arrangement
Description
Claims (7)
- 測定システムにおいて、
偏光を発生させる偏光発生部と、
前記偏光発生部で発生した偏光が特定の入射角で入射されるバイオセンサチップと、
前記バイオセンサチップより反射された反射光から、偏光信号を測定する偏光検出部とを含み、
バイオセンサチップは、高消光係数標識子と誘電体基板を用いた高感度バイオセンサチップであって、特定の入射角で入射光が入射して反射される誘電体基板と、前記基板上に固定される分析物質部と、前記分析物質部に結合され、楕円偏光信号を増幅させる高消光係数標識子とを含み、前記標識子は、特定の波長領域の光において、消光係数が特定値(k)である1.000以上であり、前記分析物質部は、前記誘電体基板の表面は、自己組立薄膜に機能化して、捕獲抗体を固定化し、標識子に付着される探知抗体と、前記探知抗体と前記捕獲抗体の間に結合して、分析対象となる生体結合物質とを含み、
前記入射角は、誘電体基板に対するブリュースター角、又は、誘電体基板のpsi値と標識子のpsi値の最大差の値となるときの入射角であることを特徴とする高消光係数標識子と誘電体基板を用いた高感度測定システム。 - 前記生体結合物質は、タンパク質、DNA、RNA、細胞、ペプチド、又はバクテリアであることを特徴とする請求項1に記載の高消光係数標識子と誘電体基板を用いた高感度バイオセンサチップ。
- 前記偏光発生部は、光源と、偏光子とを含むことを特徴とする請求項1に記載の高消光係数標識子と誘電体基板を用いた高感度測定システム。
- 前記偏光検出部は、検光子と、光検出器と、演算処理器とを含むことを特徴とする請求項1に記載の高消光係数標識子と誘電体基板を用いた高感度測定システム。
- 請求項1による高消光係数標識子と誘電体基板を用いた高感度測定システムを用いた測定方法であって、
偏光発生部で偏光を発生させるステップと、
前記偏光発生部で発生した偏光が、特定の入射角でバイオセンサチップに入射されるステップと、
誘電体基板に付着された分析物質に固定した標識子により、信号が増幅されるステップと、
前記バイオセンサチップより反射された反射光が偏光検出部に入射されて、偏光信号を測定するステップとを含むことを特徴とする高消光係数標識子と誘電体基板を用いた高感度測定方法。 - 前記標識子は、特定の波長領域の光において、消光係数kが1.000以上であることを特徴とする請求項5に記載の高消光係数標識子と誘電体基板を用いた高感度測定方法。
- 前記誘電体基板の表面は、自己組立薄膜に機能化して、捕獲抗体を固定化し、標識子に付着される探知抗体と、前記探知抗体と前記捕獲抗体の間に結合して、分析対象となる生体結合物質とを含むことを特徴とする請求項6に記載の高消光係数標識子と誘電体基板を用いた高感度測定方法。
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KR10-2018-0096767 | 2018-08-20 | ||
KR1020180096767A KR102103077B1 (ko) | 2018-08-20 | 2018-08-20 | 고소광계수 표지자와 유전체기판을 이용한 고감도 바이오센서칩, 측정시스템 및 측정방법 |
PCT/KR2019/010521 WO2020040509A1 (ko) | 2018-08-20 | 2019-08-20 | 고소광계수 표지자와 유전체기판을 이용한 고감도 바이오센서칩, 측정시스템 및 측정방법 |
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JP2011209097A (ja) | 2010-03-30 | 2011-10-20 | Fujifilm Corp | 光学的測定装置および光学的測定方法 |
JP2018503810A (ja) | 2015-06-30 | 2018-02-08 | 韓国標準科学研究院Korea Reserch Institute Of Standards And Science | 傾斜入射構造、プリズム入射型、シリコン基盤の液浸微細流路測定装置及び測定方法 |
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CN112840200B (zh) | 2024-04-02 |
WO2020040509A1 (ko) | 2020-02-27 |
US11402321B2 (en) | 2022-08-02 |
KR20200021571A (ko) | 2020-03-02 |
JP2021535379A (ja) | 2021-12-16 |
US20210072149A1 (en) | 2021-03-11 |
CN112840200A (zh) | 2021-05-25 |
KR102103077B1 (ko) | 2020-04-22 |
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