JP7136783B2 - 遺伝子操作されたグルコシルトランスフェラーゼ - Google Patents

遺伝子操作されたグルコシルトランスフェラーゼ Download PDF

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JP7136783B2
JP7136783B2 JP2019535206A JP2019535206A JP7136783B2 JP 7136783 B2 JP7136783 B2 JP 7136783B2 JP 2019535206 A JP2019535206 A JP 2019535206A JP 2019535206 A JP2019535206 A JP 2019535206A JP 7136783 B2 JP7136783 B2 JP 7136783B2
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amino acid
glucosyltransferase
seq
residue
glucan
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JP2019530474A (ja
JP2019530474A5 (enExample
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イウゲェン・リー
スーザン・マリー・ヘネシー
スラヴコ・クラール
マーク・エス・ペイン
ヴェーリ・アルカン
チィォン・チァン
ロバート・ディコジーモ
エレン・ディー・セムケ
ジャレッド・ビー・パーカー
リチャード・アール・ボット
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ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2019535206A 2016-09-14 2017-09-13 遺伝子操作されたグルコシルトランスフェラーゼ Active JP7136783B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662394354P 2016-09-14 2016-09-14
US62/394,354 2016-09-14
PCT/US2017/051279 WO2018052942A1 (en) 2016-09-14 2017-09-13 Engineered glucosyltransferases

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JP2019530474A JP2019530474A (ja) 2019-10-24
JP2019530474A5 JP2019530474A5 (enExample) 2020-10-01
JP7136783B2 true JP7136783B2 (ja) 2022-09-13

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US (4) US10301604B2 (enExample)
EP (1) EP3512955B1 (enExample)
JP (1) JP7136783B2 (enExample)
CN (1) CN109715818A (enExample)
AU (1) AU2017326168B2 (enExample)
BR (1) BR112019004213A2 (enExample)
CA (1) CA3033369A1 (enExample)
FI (1) FI3512955T3 (enExample)
WO (1) WO2018052942A1 (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018272823B2 (en) 2017-05-23 2024-05-09 Nutrition & Biosciences USA 4, Inc. Enzymatic production of alpha-1,3-glucan
EP3668970A1 (en) 2017-09-13 2020-06-24 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
AU2018334513B2 (en) * 2017-09-13 2025-02-20 Nutrition & Biosciences USA 4, Inc. Engineered glucosyltransferases
AU2018347551B2 (en) 2017-10-13 2023-09-14 Nutrition & Biosciences USA 4, Inc. Flowable bulk granular polysaccharide
CN111936570A (zh) 2017-12-14 2020-11-13 杜邦工业生物科学美国有限责任公司 α-1，3-葡聚糖接枝共聚物
EP3762486A1 (en) 2018-03-09 2021-01-13 Nutrition & Biosciences USA 4, Inc. Engineered glucosyltransferases
WO2020009893A1 (en) * 2018-07-05 2020-01-09 Dupont Nutrition Biosciences Aps Use of glucosyl transferase to provide improved texture in fermented milk based products
WO2020086935A1 (en) 2018-10-25 2020-04-30 Dupont Industrial Biosciences Usa, Llc Alpha-1,3-glucan graft copolymers
US20220033531A1 (en) 2018-12-17 2022-02-03 Dupont Industrial Biosciences Usa,Llc Polysaccharide Derivatives and Compositions Comprising Same
WO2021007264A1 (en) * 2019-07-09 2021-01-14 Nutrition & Biosciences USA 4, Inc. Engineered alpha-1,3 branching enzymes
MX2022001957A (es) 2019-08-16 2022-07-27 Nutrition & Biosciences Usa 4 Inc Productos alimentarios a base de harina de trigo y otros cereales que comprenden alfa-1,3-glucano.
CA3159763A1 (en) 2019-11-06 2021-05-14 Nutrition & Biosciences USA 4, Inc. Highly crystalline alpha-1,3-glucan
CN115052905B (zh) 2020-02-04 2024-06-11 营养与生物科学美国4公司 包含α-1,3糖苷键的不溶性α-葡聚糖的水性分散体
BR112022024705A2 (pt) 2020-06-04 2023-02-28 Nutrition & Biosciences Usa 4 Inc Composição, método para produzir um composto de éter ou éster de copolímero de enxerto, método de floculação e método de absorção
US12486338B2 (en) 2020-06-10 2025-12-02 Nutrition & Biosciences USA 4, Inc. Poly alpha-1,6-glucan derivatives and compositions comprising same
WO2022178075A1 (en) 2021-02-19 2022-08-25 Nutrition & Biosciences USA 4, Inc. Oxidized polysaccharide derivatives
EP4334364A1 (en) 2021-05-04 2024-03-13 Nutrition & Biosciences USA 4, Inc. Compositions comprising oxidized insoluble alpha-glucan
WO2022235655A1 (en) 2021-05-04 2022-11-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
US20240307288A1 (en) 2021-07-13 2024-09-19 Nutrition & Biosciences USA 4, Inc. Cationic glucan ester derivatives
CN118510405A (zh) 2021-09-30 2024-08-16 国际营养与健康丹麦私人有限公司 降低食料中糖的方法
EP4426362A1 (en) 2021-11-05 2024-09-11 Nutrition & Biosciences USA 4, Inc. Glucan derivatives for microbial control
EP4463519A1 (en) 2022-01-12 2024-11-20 Nutrition & Biosciences USA 4, Inc. Coating compositions comprising rubber and insoluble alpha-glucan
EP4496834A1 (en) 2022-03-21 2025-01-29 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
CN114717213B (zh) * 2022-05-05 2023-09-22 广西科学院 一种葡聚糖蔗糖酶的n端截短突变酶及其制备方法
JP2025523007A (ja) 2022-07-11 2025-07-17 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 両親媒性グルカンエステル誘導体
WO2024086560A1 (en) 2022-10-17 2024-04-25 International N&H Denmark Aps Method for improving flavor in plant-based food stuff
EP4611720A1 (en) 2022-11-02 2025-09-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising xanthan gum and crystalline alpha-1,3-glucan
CN120957606A (zh) 2023-03-29 2025-11-14 国际营养与健康丹麦有限公司 经由优选原位产生的α-葡聚糖改变食料质地的方法
WO2025006691A2 (en) 2023-06-30 2025-01-02 Nutrition & Biosciences USA 4, Inc. Porous alpha-1,3-glucan compositions
WO2025072419A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences Usa 1, Llc Crosslinked alpha-glucan derivatives
WO2025072416A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives
WO2025072417A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives
WO2025198996A1 (en) 2024-03-19 2025-09-25 Danisco Us Inc. Method for improving flavor in foodstuff

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003111590A (ja) 2001-10-03 2003-04-15 National Food Research Institute 改変デキストランスクラーゼ、その遺伝子組み換え体、グルカンの製造法
JP2015529096A (ja) 2012-09-25 2015-10-05 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ グルカンポリマー生成のためのグルコシルトランスフェラーゼ酵素

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1001736A1 (en) 1996-10-11 2000-05-24 Novo Nordisk A/S STARCH BINDING DOMAINS (SBDs) FOR ORAL CARE PRODUCTS
US5952205A (en) 1998-02-06 1999-09-14 Neose Technologies, Inc. Process for processing sucrose into glucose and fructose
JP2000175694A (ja) 1998-12-17 2000-06-27 Lion Corp 酵素合成によるムタンの製造方法
US7000000B1 (en) 1999-01-25 2006-02-14 E. I. Du Pont De Nemours And Company Polysaccharide fibers
US6867026B2 (en) 2000-05-25 2005-03-15 Nederlandse Organisatie Voor Toegepast Natuurwetenschappelijk Onderzoek Tno Glucosyltransferases
JP2005531511A (ja) * 2002-03-07 2005-10-20 ザ フォーシス インスティチュート グルカン結合タンパク質の免疫原性
AU2003249081B2 (en) 2002-08-06 2008-04-10 Dupont Nutrition Biosciences Aps Use of lactobacillus to produce exopolysaccharides in food and pharmaceutical compositions
US7524645B2 (en) 2004-12-14 2009-04-28 Centre National De La Recherche Scientifique (Cnrs) Fully active alternansucrases partially deleted in its carboxy-terminal and amino-terminal domains and mutants thereof
EP1679374A1 (en) 2005-01-10 2006-07-12 Bayer CropScience GmbH Transformed plant expressing a mutansucrase and synthesizing a modified starch
FR2897069B1 (fr) 2006-02-08 2012-06-08 Centre Nat Rech Scient Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.
EP2100966A1 (en) 2008-03-12 2009-09-16 Institut Pasteur Mutants of glycoside hydrolases and uses thereof for synthesizing complex oligosaccharides and disaccharide intermediates
JP5680860B2 (ja) * 2009-01-30 2015-03-04 サントリーホールディングス株式会社 フラボノイド３位ガラクトース・グルコース転移酵素、及びそれをコードするポリヌクレオチド
JP2011206273A (ja) 2010-03-30 2011-10-20 Terumo Corp 注射針組立体および薬剤注射装置
EP2566431A1 (en) 2010-05-04 2013-03-13 Sanofi-Aventis Deutschland GmbH Tactile identification of drug filled cartridge
JP5015287B2 (ja) 2010-05-11 2012-08-29 シャープ株式会社 加熱調理器
JP5851149B2 (ja) 2011-08-08 2016-02-03 株式会社ニューフレアテクノロジー 成膜装置および成膜方法
US8642757B2 (en) 2011-09-09 2014-02-04 E I Du Pont De Nemours And Company High titer production of highly linear poly (α 1,3 glucan)
US9080195B2 (en) 2011-09-09 2015-07-14 E I Du Pont De Nemours And Company High titer production of poly (α 1,3 glucan)
CN104968781A (zh) * 2012-12-24 2015-10-07 诺维信公司 具有内切葡聚糖酶活性的多肽以及对其进行编码的多核苷酸
US9169506B2 (en) 2013-09-05 2015-10-27 E I Du Pont De Nemours And Company Process for producing alpha-1,3-glucan polymer with reduced molecular weight
US9644190B2 (en) 2014-02-06 2017-05-09 The United States Of America, As Represented By The Secretary Of Agriculture Modified glucansucrase and related methods
WO2015123327A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Glucosyltransferase enzymes for production of glucan polymers
US20150232785A1 (en) 2014-02-14 2015-08-20 E I Du Pont De Nemours And Company Polysaccharides for viscosity modification
MX2016015604A (es) 2014-05-29 2017-07-04 Du Pont Síntesis enzimática de fibra soluble de glucano.
WO2015183714A1 (en) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
BR112016027867A2 (pt) 2014-05-29 2017-10-24 Du Pont composições de fibra e de carboidrato, produto alimentício, métodos para produzir uma composição de fibra, de carboidrato, para reduzir o índice glicêmico e para inibir a elevação do nível de açúcar, composição cosmética, uso da composição de fibra e composição
CA2949278A1 (en) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
MX2016015703A (es) 2014-05-29 2017-03-16 Du Pont Sintesis enzimatica de fibra de glucano soluble.
WO2015183724A1 (en) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
JP6814737B2 (ja) 2014-11-05 2021-01-20 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 酵素重合ゲル化デキストラン
EP3310906B1 (en) 2015-06-17 2020-05-06 DuPont Industrial Biosciences USA, LLC Modified glucosyltransferases for producing branched alpha-glucan polymers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003111590A (ja) 2001-10-03 2003-04-15 National Food Research Institute 改変デキストランスクラーゼ、その遺伝子組み換え体、グルカンの製造法
JP2015529096A (ja) 2012-09-25 2015-10-05 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ グルカンポリマー生成のためのグルコシルトランスフェラーゼ酵素

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US20190292528A1 (en) 2019-09-26
US11028373B2 (en) 2021-06-08
AU2017326168B2 (en) 2022-02-24
US20240417702A1 (en) 2024-12-19
JP2019530474A (ja) 2019-10-24
EP3512955B1 (en) 2023-04-19
US20180072998A1 (en) 2018-03-15
WO2018052942A1 (en) 2018-03-22
US20210395705A1 (en) 2021-12-23
US11952598B2 (en) 2024-04-09
US12378535B2 (en) 2025-08-05
BR112019004213A2 (pt) 2019-06-25
EP3512955A1 (en) 2019-07-24
AU2017326168A1 (en) 2019-02-21
FI3512955T3 (fi) 2023-07-18
CA3033369A1 (en) 2018-03-22
CN109715818A (zh) 2019-05-03
US10301604B2 (en) 2019-05-28

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