CA3033369A1 - Engineered glucosyltransferases - Google Patents

Engineered glucosyltransferases Download PDF

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Publication number
CA3033369A1
CA3033369A1 CA3033369A CA3033369A CA3033369A1 CA 3033369 A1 CA3033369 A1 CA 3033369A1 CA 3033369 A CA3033369 A CA 3033369A CA 3033369 A CA3033369 A CA 3033369A CA 3033369 A1 CA3033369 A1 CA 3033369A1
Authority
CA
Canada
Prior art keywords
amino acid
seq
alpha
glucosyltransferase
glucan
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3033369A
Other languages
English (en)
French (fr)
Inventor
Yougen Li
Susan Marie Hennessey
Slavko Kralj
Mark S. Payne
Veli Alkan
Qiong Cheng
Robert Dicosimo
Ellen D. SEMKE
Jared B. PARKER
Richard R. Bott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA3033369A1 publication Critical patent/CA3033369A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3033369A 2016-09-14 2017-09-13 Engineered glucosyltransferases Abandoned CA3033369A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662394354P 2016-09-14 2016-09-14
US62/394,354 2016-09-14
PCT/US2017/051279 WO2018052942A1 (en) 2016-09-14 2017-09-13 Engineered glucosyltransferases

Publications (1)

Publication Number Publication Date
CA3033369A1 true CA3033369A1 (en) 2018-03-22

Family

ID=59969237

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3033369A Abandoned CA3033369A1 (en) 2016-09-14 2017-09-13 Engineered glucosyltransferases

Country Status (9)

Country Link
US (4) US10301604B2 (enExample)
EP (1) EP3512955B1 (enExample)
JP (1) JP7136783B2 (enExample)
CN (1) CN109715818A (enExample)
AU (1) AU2017326168B2 (enExample)
BR (1) BR112019004213A2 (enExample)
CA (1) CA3033369A1 (enExample)
FI (1) FI3512955T3 (enExample)
WO (1) WO2018052942A1 (enExample)

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JP2024517798A (ja) 2021-05-04 2024-04-23 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 不溶性アルファ－グルカンを含む組成物
CN117616054A (zh) 2021-07-13 2024-02-27 营养与生物科学美国4公司 阳离子葡聚糖酯衍生物
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WO2023183284A1 (en) 2022-03-21 2023-09-28 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
CN114717213B (zh) * 2022-05-05 2023-09-22 广西科学院 一种葡聚糖蔗糖酶的n端截短突变酶及其制备方法
CN119907814A (zh) 2022-07-11 2025-04-29 营养与生物科学美国第四公司 两亲性葡聚糖酯衍生物
AU2023365666A1 (en) 2022-10-17 2025-03-27 Danisco Us Inc. Method for improving flavor in plant-based food stuff
EP4611720A1 (en) 2022-11-02 2025-09-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising xanthan gum and crystalline alpha-1,3-glucan
CN120957606A (zh) 2023-03-29 2025-11-14 国际营养与健康丹麦有限公司 经由优选原位产生的α-葡聚糖改变食料质地的方法
WO2025006691A2 (en) 2023-06-30 2025-01-02 Nutrition & Biosciences USA 4, Inc. Porous alpha-1,3-glucan compositions
WO2025072419A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences Usa 1, Llc Crosslinked alpha-glucan derivatives
WO2025072416A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives
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Also Published As

Publication number Publication date
JP2019530474A (ja) 2019-10-24
BR112019004213A2 (pt) 2019-06-25
AU2017326168B2 (en) 2022-02-24
US10301604B2 (en) 2019-05-28
US20190292528A1 (en) 2019-09-26
FI3512955T3 (fi) 2023-07-18
JP7136783B2 (ja) 2022-09-13
AU2017326168A1 (en) 2019-02-21
CN109715818A (zh) 2019-05-03
US11028373B2 (en) 2021-06-08
US11952598B2 (en) 2024-04-09
EP3512955B1 (en) 2023-04-19
US20240417702A1 (en) 2024-12-19
US20180072998A1 (en) 2018-03-15
US20210395705A1 (en) 2021-12-23
WO2018052942A1 (en) 2018-03-22
EP3512955A1 (en) 2019-07-24
US12378535B2 (en) 2025-08-05

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20220315

FZDE Discontinued

Effective date: 20220315