JP7078925B2 - 毛包上皮幹細胞の培養方法及び培養キット - Google Patents

毛包上皮幹細胞の培養方法及び培養キット Download PDF

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JP7078925B2
JP7078925B2 JP2018020376A JP2018020376A JP7078925B2 JP 7078925 B2 JP7078925 B2 JP 7078925B2 JP 2018020376 A JP2018020376 A JP 2018020376A JP 2018020376 A JP2018020376 A JP 2018020376A JP 7078925 B2 JP7078925 B2 JP 7078925B2
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hair follicle
epithelial stem
stem cells
follicle epithelial
culture
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JP2019135947A5 (https=
JP2019135947A (ja
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淳二 福田
達斗 景山
杉 平野
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Yokohama National University NUC
Kanagawa Institute of Industrial Science and Technology
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Yokohama National University NUC
Kanagawa Institute of Industrial Science and Technology
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Priority to US16/968,323 priority patent/US20210079345A1/en
Priority to EP19751917.6A priority patent/EP3750986A4/en
Priority to PCT/JP2019/003903 priority patent/WO2019156032A1/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C12N5/0628—Hair stem cells; Hair progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L83/00—Compositions of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon only; Compositions of derivatives of such polymers
    • C08L83/04—Polysiloxanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Dermatology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2018020376A 2018-02-07 2018-02-07 毛包上皮幹細胞の培養方法及び培養キット Active JP7078925B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2018020376A JP7078925B2 (ja) 2018-02-07 2018-02-07 毛包上皮幹細胞の培養方法及び培養キット
US16/968,323 US20210079345A1 (en) 2018-02-07 2019-02-04 Method and Kit for Culturing Hair Follicle's Epithelial Stem Cells
EP19751917.6A EP3750986A4 (en) 2018-02-07 2019-02-04 PROCESS AND KIT FOR CULTURE OF EPITHELIAL STEM CELLS OF THE PILEOUS FOLLICLE
PCT/JP2019/003903 WO2019156032A1 (ja) 2018-02-07 2019-02-04 毛包上皮幹細胞の培養方法及び培養キット

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JP2018020376A JP7078925B2 (ja) 2018-02-07 2018-02-07 毛包上皮幹細胞の培養方法及び培養キット

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JP2019135947A JP2019135947A (ja) 2019-08-22
JP2019135947A5 JP2019135947A5 (https=) 2021-03-11
JP7078925B2 true JP7078925B2 (ja) 2022-06-01

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US (1) US20210079345A1 (https=)
EP (1) EP3750986A4 (https=)
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WO (1) WO2019156032A1 (https=)

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JPWO2020066818A1 (ja) * 2018-09-26 2021-11-25 株式会社オーガンテクノロジーズ 毛包上皮性幹細胞の生体外増殖方法
JP7372621B2 (ja) * 2019-09-30 2023-11-01 京セラ株式会社 容器
WO2021162206A1 (ko) * 2020-02-10 2021-08-19 주식회사 마이크로바이오틱스 모낭조직 배양액을 포함하는 탈모의 예방 또는 치료용 조성물
WO2022191812A1 (en) * 2021-03-08 2022-09-15 National Cheng Kung University Method of facilitating wound-induced hair follicle neogenesis, tissue regeneration and reducing wound stiffness

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008125540A (ja) 2006-11-16 2008-06-05 Lion Corp 人工皮膚
JP2009529886A (ja) 2006-03-17 2009-08-27 インターサイテックス リミティド 毛嚢生成のための細胞同時培養
JP2012249556A (ja) 2011-06-01 2012-12-20 Nara Medical Univ 毛包幹細胞の培養方法
WO2017070506A1 (en) 2015-10-21 2017-04-27 Indiana University Research And Technology Corporation Derivation of human skin organoids from pluripotent stem cells
WO2017073625A1 (ja) 2015-10-30 2017-05-04 国立大学法人横浜国立大学 再生毛包原基の集合体の製造方法、毛包組織含有シート、及び毛包組織含有シートの製造方法
WO2017217393A1 (ja) 2016-06-17 2017-12-21 国立大学法人横浜国立大学 毛髪再生用細胞包埋ビーズ及びその製造方法、並びに毛髪再生用キット

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3951148B2 (ja) * 1996-10-22 2007-08-01 東洋紡績株式会社 皮膚付属器官様構造体を含む人工皮膚及びその製造方法
JP2005218445A (ja) * 2004-01-09 2005-08-18 Technology Seed Incubation Co Ltd 培養皮膚細胞とこれを原料として用いた移植用材及び遺伝子解析用材並びに培養皮膚細胞の製造方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009529886A (ja) 2006-03-17 2009-08-27 インターサイテックス リミティド 毛嚢生成のための細胞同時培養
JP2008125540A (ja) 2006-11-16 2008-06-05 Lion Corp 人工皮膚
JP2012249556A (ja) 2011-06-01 2012-12-20 Nara Medical Univ 毛包幹細胞の培養方法
WO2017070506A1 (en) 2015-10-21 2017-04-27 Indiana University Research And Technology Corporation Derivation of human skin organoids from pluripotent stem cells
WO2017073625A1 (ja) 2015-10-30 2017-05-04 国立大学法人横浜国立大学 再生毛包原基の集合体の製造方法、毛包組織含有シート、及び毛包組織含有シートの製造方法
WO2017217393A1 (ja) 2016-06-17 2017-12-21 国立大学法人横浜国立大学 毛髪再生用細胞包埋ビーズ及びその製造方法、並びに毛髪再生用キット

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHACON-MARTINEZ Carlos Andres, et al.，Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and their progeny.，The EMBO Journal，2017年，Vol.36, No.2，p.151-164
Chin-Hsiung Hsieh, et al.，Large-scale cultivation of transplantable dermal papilla cellular aggregates using microfabricated PDMS arrays.，Acta Biomaterialia，2011年，Vo.7，p.315-324
高分子材料の酸素透過係数に関する分子構造要因解析手法，デンソーテクニカルレビュー，2007年，Volume 12, Number 2，pp. 98-102，https://www.denso.com/jp/ja/-/media/global/business/innovation/review/12-2/12-2-doc-15-ja.pdf

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EP3750986A1 (en) 2020-12-16
EP3750986A4 (en) 2021-11-10
WO2019156032A1 (ja) 2019-08-15
US20210079345A1 (en) 2021-03-18
JP2019135947A (ja) 2019-08-22

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