JP7062667B2 - コラーゲンヒドロゲルの製造方法 - Google Patents
コラーゲンヒドロゲルの製造方法 Download PDFInfo
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- JP7062667B2 JP7062667B2 JP2019533705A JP2019533705A JP7062667B2 JP 7062667 B2 JP7062667 B2 JP 7062667B2 JP 2019533705 A JP2019533705 A JP 2019533705A JP 2019533705 A JP2019533705 A JP 2019533705A JP 7062667 B2 JP7062667 B2 JP 7062667B2
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- collagen
- cells
- jellyfish
- collagen hydrogel
- purified
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Description
したがって、コラーゲンの代替の供給源が望ましいと、ずっと考えられてきた。
しかし、クラゲコラーゲンに課せられた進化上の制約は、タンパク質が、より低い温度で最適に機能するように進化したことである。このより低い熱安定性のために、生理的温度での使用に適切なクラゲコラーゲンヒドロゲルの生産がこれまで不可能であった。
そのため、細胞培養及び医療用途における使用に適切な、改善されたクラゲコラーゲンヒドロゲルの製造方法が求められていた。
クラゲコラーゲンの抽出
コクカイビゼンクラゲ(Rhizostoma pulmo)からの酸可溶性コラーゲンの抽出
クラゲ(コクカイビゼンクラゲ:Rhizostoma pulmo)材料を、400μmフィルターを通し、保持物を、後で使用するために凍結した。凍結された組織を、通常の攪拌によって、24時間かけて解凍した。完全に解凍したらすぐに、組織を、50%NaOHでpH13にし、さらに24時間、放置した。24時間後、濃酢酸を、濃度0.5Mまで加え、pHを、HClを使用して、3.0に調整した。次に、組織を、3~5日間、放置して、非常に低い固体含有量の粘性溶液を得た。全ての工程を、4℃で実行した。
溶液を、20000RCFで1時間、遠心分離して、全ての残っている固体物質を除去した。遠心分離後、濃度1MまでNaClを添加することによって、コラーゲンを、溶液から沈殿させ、引き続き、さらに20分間、遠心分離して、沈殿させたコラーゲンを得た。次に、ペレットを、0.1M酢酸中で再懸濁し、濃HClによって、pHをpH3に調整した。結果として生じる溶液を、合計10時間、さらに遠心分離し、次に、無色透明のJFC溶液が得られるまで、100kDa濾過膜を組み込んだSartorius Vivaflow200システムによって、0.1M酢酸で6倍量の透析容量で透析濾過した。この場合も先と同様に、全ての工程を、4℃で実行した。
JFCヒドロゲル形成に対する溶液条件の影響
JFCの原線維発生アッセイ-酸可溶性のpH依存
酸可溶性JFCを、3mg/mlに希釈し、10×リン酸緩衝生理食塩水(PBS)に加えて、9:1の比率にした。試料のpHを、1MのNaOHを使用して、pH3、5、6、7、8、及び9に調整した(図1)。次に、試料を、UV-visキュベットに加えて3通り作り、定常に到達するまで、313nmで、通常の吸光度測定を行った。
酸可溶性JFCを、3mg/mlに希釈し、溶液のpHを、1MのNaOHを使用して、pH7.4に調整した。次に、0M、0.69M、1.38M及び2.74MのNaClを含有する10×PBSを加えて、JFC対10×PBS比、9:1にし、必要であれば、pHを、再びpH7.4に調整した。次に、試料を、UV-visキュベットに加えて3通り作り、定常に到達するまで、313nmで、通常の吸光度測定を行った(図2)。
酸可溶性JFCの試料3mg/mlに、10×PBSを加えて、JFC対PBS比、9:1にすることによって、JFCを、ゲル状にした。NaOHを加えて、溶液をpH3、5、6、7、8、及び9に調整した。次に、それぞれのpHの試料を、8時間、4℃又は19℃のいずれかで放置して、ゲルを成長させた。
ヒドロゲルの熱安定性を、pH9及び4℃で、PBS中に形成されるヒドロゲルを使用して研究した。JFC溶液に、2.74MのNaClを補充した。結果として生じるJFCヒドロゲルを、18℃、20℃、22℃、24℃、及び26℃のいずれかで、5分間、水浴槽に浸漬した。変性温度を、ゲルの半分が崩壊する時点として、判定した。
ヒドロゲルの熱安定性を上昇させること
クラゲコラーゲン(JFC)ヒドロゲルの熱安定性を上昇させるクロスリンカーである、ゲニピン、BDDGE、及びムコクロロ酸の能力を、>37℃で長期間安定なJFCヒドロゲルを作る目的で、評価した。
酸で可溶化されたJFC3.5mg/mlの複数のアリコートを、10×リン酸緩衝生理食塩水(PBS)で、9:1(JFC:10×PBS)の比率で希釈した。次に、それぞれの溶液を、濃NaOHを加えることによって、pH3からpH9の間にpH調整した。次に、架橋剤1,4-BDDGE、ゲニピン又はムコクロロ酸を加えて、それぞれ、最終濃度1%から4%まで、又は0.025%から5%まで、又は0.1%から5%にした。次に、溶液を、攪拌して、クロスリンカーを拡散させ、0.5時間から24時間、4℃から25℃でインキュベートして、原線維発生及びゲル化を完成させた。
熱安定性を判定するために、架橋ゲルを、20℃のインキュベーターに入れた。温度を、1時間ごとに1℃上昇させて、熱安定性を記録した(図3)。熱安定性を、ゲルが最初に収縮及び/又は融解し始めた温度と定義した、以下、Tmと呼ぶ。
ゲニピン及びBDDGEの両方の2つの濃度を、使用して、37℃で安定なヒドロゲルを形成させ得る、それぞれのクロスリンカーの可能な最低濃度を判定した。架橋ヒドロゲルを、120時間、pH7で、1%及び4%BDDGE(v/v)ならびに0.025%、0.05%、0.075%及び0.1%ゲニピンで形成し、Tmに対する効果を、(図4)に示す。図で分かる通り、1%BDDGEヒドロゲルは、4%BDDGEヒドロゲルと全く同じTmを有した。両方の濃度は、非架橋ヒドロゲルにおける20℃から、1%及び4%架橋ヒドロゲル両方における35℃まで、Tmの上昇15℃をもたらした。pH8でインキュベートされなかったので、いずれの濃度も、37℃で安定なヒドロゲルを生成しなかった。ゲニピン濃度の全ては、37℃で熱安定性のヒドロゲルを生成し、最低Tmは、0.025%ゲニピン架橋ヒドロゲルにおける43℃であった(図4)。これは、非架橋対照より23℃高い。3つのより高い濃度、0.05%、0.075%及び0.1%GPは全て、Tm45℃を有した。
Claims (22)
- クラゲコラーゲン原線維を含むクラゲコラーゲンヒドロゲルの製造方法であって、前記製造方法は、
a. i.精製されたクラゲコラーゲンの溶液、及び
ii.水性中和緩衝液を混合する工程と、
b.前記混合物を、コラーゲン原線維の形成を可能にするのに十分な長さの時間、インキュベートする工程と、を含み、
架橋剤が、混合工程(a)の間に加えられるか、又は工程(b)から得られた前記コラーゲン原線維に加えられるかのいずれかであるコラーゲンヒドロゲルの製造方法。 - 前記混合工程(a)が、pH4からpH9のpHを有する溶液をもたらす、場合によりpHは7.4である、請求項1に記載のコラーゲンヒドロゲルの製造方法。
- 工程(b)が、4℃から25℃の温度で実施される、請求項1又は2に記載のコラーゲンヒドロゲルの製造方法。
- 前記十分な長さの時間が、12時間以下であり、好ましくは、前記十分な長さの時間が、5から60分である、請求項1から3のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記水性中和緩衝液が、リン酸をベースとする、請求項1から4のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記架橋剤が、ゲニピン、1,4-BDDGE、又はムコクロロ酸から選択されるものであり、
前記架橋剤が、
a.ゲニピンの場合、最終濃度0.001%から5%、好ましくは0.025%(w/v)で加えられ、
b.1,4-BDDGEの場合、最終濃度0.001%から5%、好ましくは4%(w/v)で加えられ、
c.ムコクロロ酸の場合、最終濃度0.001%から5%、好ましくは、0.25%から4%(w/v)で加えられ、
場合により、前記架橋剤がゲニピン又は1,4-BDDGEである、請求項1から5のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。 - 前記精製されたクラゲコラーゲンの溶液が、酵素抽出によってコラーゲンの供給源から精製されたものであり、場合により前記酵素がペプシンである、請求項1から6のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記精製されたクラゲコラーゲンの溶液が、酸抽出によってコラーゲンの供給源から精製された、請求項1から7のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記精製されたクラゲコラーゲンの溶液が、コクカイビゼンクラゲ(Rhizostomas pulmo)、ビゼンクラゲ(Rhopilema esculentum)、ロピレマ・ノマディカ(Rhopilema nomadica)、スナクラゲ(Stomolophus meleagris)、ミズクラゲ(Aurelia sp.)、エチゼンクラゲ(Nemopilema nomurai)、又はそれらの組合せから精製されたものであり、場合により前記精製されたクラゲコラーゲンの溶液が、コクカイビゼンクラゲ(Rhizostomas pulmo)から精製された、請求項1から8のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 請求項1から9のいずれか一項に記載のコラーゲンヒドロゲルの製造方法から得ることができるものであり、前記コラーゲンヒドロゲルは25℃から50℃の温度で安定であるクラゲコラーゲンヒドロゲル。
- 25℃から50℃の温度で安定な単離されたものであり、前記コラーゲンヒドロゲルは中和緩衝液及び架橋剤を含み、前記コラーゲンヒドロゲルはクラゲコラーゲン原線維を含むクラゲコラーゲンヒドロゲル。
- 3D細胞培養足場の生産における、請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルの使用。
- 医療用デバイスの生産において、請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルの使用であって、場合により前記医療用デバイスが、創傷被覆材である、使用。
- 薬物送達のための担体としての、請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルの使用。
- 請求項10又は請求項11に記載の前記クラゲコラーゲン足場を含む医療用デバイスであって、場合により前記医療用デバイスが創傷被覆材である、医療用デバイス。
- 請求項10又は請求項11に記載のクラゲコラーゲンヒドロゲルを含む細胞培養足場。
- 請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルを含む薬物送達のための担体。
- 細胞を培養するための、請求項16に記載の細胞培養足場の使用であって、場合により前記細胞がヒトの細胞である細胞培養足場の使用。
- 前記細胞が、肝細胞、筋細胞、心筋細胞、ケラチノサイト、脂肪細胞、ニューロン、腎細胞、上皮細胞、グリア細胞、ホルモン分泌細胞、バリア機能細胞、細胞外マトリックス細胞、収縮性細胞、水晶体細胞、幹細胞、間葉系幹細胞、血液由来の幹細胞、人工多能性幹細胞、又はそれらの組合せからなる群から選択される、初代哺乳類細胞であって、
場合により前記細胞が肝細胞又は心筋細胞である、請求項18に記載の使用。 - (i)前記細胞が、培養されて、創薬における使用のための3D細胞組織構造を形成する、
(ii)前記細胞が、培養されて、角膜シールドを形成する、
(iii)前記細胞が、培養されて、軟膏を生成する、
(iv)前記細胞が、培養されて、骨を形成する、
(v)前記細胞が、培養されて、ニューロンを形成する、
(vi)前記細胞が、共培養できる、
請求項18に記載の使用。 - 請求項1に記載のコラーゲンヒドロゲルの製造方法に使用するためのキットであって、
前記キットは、精製されたクラゲコラーゲンの溶液、中和緩衝液及び架橋剤を含み、場合により前記精製されたクラゲコラーゲンの溶液が、0.1mg/mlから30mg/mlの範囲内の濃度であるキット。 - 請求項21に記載のコラーゲンヒドロゲルの製造方法に使用するためのキットであって、
前記中和緩衝液が、遊離アミンを含まず、好ましくは、前記中和緩衝液が、リン酸をベースとするものであり、場合により前記架橋剤が、ゲニピン、1,4-BDDGE、又はムコクロロ酸から選択されるキット。
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Also Published As
Publication number | Publication date |
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ES2870612T3 (es) | 2021-10-27 |
KR102606472B1 (ko) | 2023-11-28 |
DK3509649T3 (en) | 2021-04-06 |
JP2019529538A (ja) | 2019-10-17 |
PT3509649T (pt) | 2021-06-02 |
EP3509649A1 (en) | 2019-07-17 |
WO2018046920A1 (en) | 2018-03-15 |
KR20230163000A (ko) | 2023-11-29 |
US20190216972A1 (en) | 2019-07-18 |
GB201615205D0 (en) | 2016-10-19 |
US11369714B2 (en) | 2022-06-28 |
KR20190055124A (ko) | 2019-05-22 |
EP3509649B1 (en) | 2021-02-24 |
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