JP7057348B2 - 蛍光in situ配列決定を用いた単一アッセイに生体分子の検出を組み合わせる方法 - Google Patents
蛍光in situ配列決定を用いた単一アッセイに生体分子の検出を組み合わせる方法 Download PDFInfo
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Description
本出願は、2016年8月31日に出願された米国仮特許出願第62/381,997号の利益を主張し、この出願はその全体が参照により本明細書に援用される。
本発明は、アメリカ国立衛生研究所(National Institutes of Health)により授与された助成金番号P50HG005550及びRM1 HG008525及びアメリカ国立科学財団(National Science Foundation)により授与された助成金番号DGE1144152の下での政府の支援によりなされた。米国政府は本発明において一定の権利を有する。
本明細書で言及された全ての刊行物、特許、及び特許出願は、それぞれ個々の刊行物、特許、又は特許出願が具体的かつ個別に参照により援用されることが示されるのと同程度に参照により本明細書に援用される。
本開示は、目的の生体分子が三次元マトリックス内に固定されるように試料を処理することに関するシステム及び方法、を提供する。本明細書で使用される三次元マトリックスは、ヒドロゲル又はゲルを指し得る。三次元マトリックスは、電磁気刺激、電気化学的刺激、又は熱刺激などの様々な刺激を使用して重合及び/又は膨張させることができる。いくつかの実施形態において、三次元マトリックスは、電磁気刺激、電気化学的刺激、又は熱刺激などの外部刺激によって拡大又は縮小/収縮することができる。いくつかの例において、三次元マトリックスを重合し、続いて目的の生体分子を検出するために標識を含むプローブをマトリックスに流し込んでもよい。試料は、単一アッセイでin situで異なるクラスの生体分子を検出するために使用及び/又は再使用することができる。いくつかの例において、試料(同一試料)は、異なる種類又はクラスの生体分子の検出のために複数回処理又はアッセイされてもよい。本開示のシステム及び方法の利点の1つは、例えば、異なる生体分子検出のために単一の試料を使用することである。いくつかの実施形態において、試料は、ハイドロゲルがその場で形成され得るように試薬(例えば、ハイドロゲル形成モノマー)によって処理される。いくつかの実施形態において、目的の生体分子は、核酸、タンパク質、及び/又は小分子であり得る。様々な場合において、目的の生体分子は、付着部分を介してハイドロゲルに連結している。様々な実施形態において、ハイドロゲルを調製するための試薬が提供される。様々な実施形態において、目的の異なる生体分子をハイドロゲルに結合する方法が提供される。いくつかの例において、容器は、本明細書に記載のシステムの一部として提供され得る。容器は、生体試料、生体分子、膨潤剤、付着部分、試薬などを収容するように構成された任意の容器であり得る。場合によっては、容器は刺激の供給源から刺激を受け取るように構成され得る。
蛍光in situ配列決定(FISSSEQ)は、マトリックス内でin situで三次元的に配置された標的を検出又は配列決定する方法を指すことができ、ここで検出シグナルは蛍光シグナルである。FISSEQによって採用され得る配列決定方法は、合成による配列決定、ライゲーションによる配列決定、又はハイブリダイゼーションによる配列決定であり得る。FISSEQで検出又は配列決定された標的は、目的の生体分子又は目的の生体分子に結合したプローブであり得る。
次世代配列決定技術は、DNA配列の空間的構成に関する情報を必要としない場合があるので、例えば個別化医療のために、そして系統発生を推論することなどの他のゲノム配列決定用途のために、生物間の多様な変異源を再配列決定するために使用できる。しかしながら、ゲノムFISSEQを使用して、生体試料内の非常に貴重な多数の情報を取得することができる。
抗体又は他のマーカーは、OligoPaint、OligoFISSEQと併用したDNAコンジュゲート抗体の使用、環状化法による捕捉、又は直接的in situゲノム配列決定などによるゲノム検出と並行したエピジェネティック(後成的)な修飾に適用できる。抗体が利用可能であり、遺伝子制御、転写、複製、及び/又はDNA損傷及び修復において顕著な役割を果たす因子には、Oct4、Sox2、及びNanogなどの多能性の確立に関与する要因と同様に、コヒーシン、コンデンシン、RNAPII、CTCF、ヒストン変異体(例えば、H2A.Z、H2A.X、H3K4me3、H3K27ac、H3K27me3、H3K9me2/3)、PRC1、PRC2、SIN3、NuRDの成分、及びco-RESTクロマチン複合体などの包括的に作用する因子が含まれる。ゲノムFISSEQは相同染色体の視覚的識別を可能にするので、我々はX不活性化、インプリンティング、及び単一対立遺伝子発現を検討できる。重要なことに、他の相同体感受性法はゲノム又はRNA分子の反復部分に限定され、したがって単一コピー又は発現抑制領域には不適切であるが、本明細書に提供される方法は一塩基多型(SNP)を標的とし、ゲノム全体の発見を可能にする。
従来のタンパク質検出アッセイの限界
核酸標的のように、タンパク質はin situで検出され得る。タンパク質を標的とするために様々な方法を使用することができる。例えば、in situタンパク質検出は、免疫タンパク質及びアプタマーの親和性結合特性を利用し得る。
いくつかの実施形態において、本明細書に提供されるのは、FISSEQに使用される親和性バインダーのライブラリー・オン・ライブラリー選択を使用する方法である。いくつかの実施形態において、ライブラリー・オン・ライブラリー選択戦略は、単一分子相互作用配列決定(SMI-seq)を使用することであり得る。この戦略は、同じ緩衝液条件で機能し得る複数のタンパク質標的に対して複数のバインダーを選択するのに有用であり得る。いくつかの実施形態において、本明細書に提供される方法において選択されるバインダーは、同じ条件で作用し得、そして他のものと相互作用しない。いくつかの実施形態において、バインダーは互いに反応性が低下している可能性がある。本明細書に提供される方法は、選択されたバインダーを使用し、FISSEQを使用して三次元的に固定した試料中のタンパク質を検出する。
いくつかの実施形態において、本明細書に提供される方法は、タンパク質配列を同定することをさらに含む。いくつかの実施形態において、タンパク質は、エドマン分解によって同定され得る。
本開示は、蛍光部分に結合した複数種の準安定自己組織化DNAヘアピン、例えばハイブリダイゼーション連鎖反応モノマーを含むタンパク質FISSEQライブラリーの蛍光in situ配列決定のためのキット又はシステムを提供する。いくつかの実施形態において、キットは、親和性バインダーバーコード配列に相補的な配列を含む複数種のDNAオリゴヌクレオチドを含む。いくつかの実施形態において、キットは、イメージング用緩衝液を含む。いくつかの実施形態において、キットは、ハイブリダイゼーション用緩衝液を含む。いくつかの実施形態において、キットは、HCR増幅用緩衝液を含む。
真のパンオミクスin situ分子検出技術を創出するために、核酸及びタンパク質に加えて他のクラスの生体分子を本明細書に提供される方法において標的とすることができる。いくつかの従来の小分子検出方法は、本明細書に提供されているFISSEQと組み合わせることができる。代謝産物は、本質的には細胞生化学過程と相互作用するあらゆる小分子であり、ビタミン、アミノ酸、ヌクレオチド、有機酸、アルコール及びポリオール、脂質並びに脂肪酸などが含まれる(Wishart, David S., et al. “HMDB: the human metabolome database.” Nucleic acids research 35.suppl 1 (2007): D521-D526)。代謝における中間体、生成物、及び補因子としてのそれらの役割を超えて、代謝産物及び他の小分子は、エネルギー源、シグナル伝達分子、浸透圧調節剤、及び酵素阻害剤又は活性化剤として生物系において役割を果たすことができる。代謝産物はまた、毒素、顔料、臭気物質、フェロモンなどとして、組織、生物全体、個体群、及び生態学の規模で重要な機能を果たす(Vining, Leo C. “Functions of secondary metabolites.” Annual Reviews in Microbiology 44.1 (1990): 395-427)。特に重要なクラスの代謝産物の一つは脂質である。脂質種の数はタンパク質種の数とほぼ同じで、脂質は構造分子及びシグナル分子として様々な役割を果たす(Muro, Eleonora, G. Ekin Atilla-Gokcumen, and Ulrike S. Eggert. “Lipids in cell biology: how can we understand them better?. “Molecular biology of the cell 25.12 (2014): 1819-1823)。これら全ての小分子は、細胞内で複雑な空間パターンの構成を有し得る。
いくつかの場合において、生体分子間の相互作用を検出することができる。分子相互作用の強度を検出及び測定するためのいくつかの戦略があり、一般的に4つのテーマに分類することができる:アレイ化検体への結合の定量、架橋精製、相補性アッセイ、及び単一分子イメージング。
染色は、典型的には試料のいくつかの成分の異なる結合によって、生体試料の特徴間のコントラストを増強するための重要な技術であり得る。合成アニリン染料の発見以来、科学者達は化学反応と化学成分及び組織成分間の親和性を利用して、組織構造及び化学的及び分子的組成の視覚的又は顕微鏡的分析のために組織の光学的性質を高めた。最もよく知られている染色の1つは、ヘマトキシリン及びエオシン(H&E)の組み合わせであり得、それぞれ核酸及び細胞質/細胞外組織成分を染色する。その他の染色の例としては、トルイジンブルー、マッソン(Masson)のトリクローム染色、マロリー(Mallory)のトリクローム染色、ワイゲルト(Weigert)の弾性染色、ハイデンハイン(Heidenhain)のアザントリクローム染色、シルバー(Silver)染色、ライト(Wright)染色、オルセイン(Orcein)染色、及び過ヨウ素酸シッフ(periodic-acid Schiff:PAS)染色が挙げられる。DAPI及び他の挿入染料もまた、核酸及び核を標識するために使用してもよい。
いくつかの場合において、より高い解像度のイメージングが必要とされている。生体システム内では、ほとんどの物質はナノメートルオーダーの大きさである。例えば、DNAらせんの「B」型は、10塩基対当たり23.7オングストローム幅及び34オングストローム長である。例えば、Watson, James D., and Francis HC Crick. “Molecular structure of nucleic acids.“ Nature 171.4356 (1953): 737-738を参照。最小のポリペプチドも数ナノメートルのオーダーの長さである。したがって、生物学における「完全な分解能」は数ナノメートルのオーダーになる。蛍光イメージングのために、任意の数の戦略を使用してこの解像度を達成することが可能である。
分子及びプローブ捕捉
FISSEQハイドロゲルを形成するためには、特定の性質が望まれ得る。例えば、酵素との相溶性、急速拡散、熱的、物理的、光及び化学的安定性、光学的透明度、核酸との共有結合を確立するための入手容易な化学、固体支持基板への付着方法、生体直交性、及び均一なナノスケールネットワークアーキテクチャである。
配列決定は、実質的に無制限の固有のアイデンティティを持つ豊富なデジタルラベルとして機能する一連の順序付けられたシグナルを取得するプロセスである。核酸分子内の塩基の配列を決定するための、又は核酸を使用して蛍光シグナルの順序付けられたセットを生成するための化学として、ハイブリダイゼーションによる配列決定(SBH)、ライゲーションによる配列決定(SBL)、及び合成による配列決定(SBS)が挙げられるが、これらに限定されない。
パンオミクスFISSEQは、試料内の同一の物理的空間に対応し、試料を構成する一連の画像を特徴とする一種のデータを生成することができる。
本明細書で使用される核酸化学、生化学、遺伝学、及び分子生物学の用語及び記号は、当技術分野の、例えば以下の標準的な論文及び教科書に従う:Komberg and Baker, DNA Replication, Second Edition (W.H. Freeman, New York, 1992); Lehninger, Biochemistry, Second Edition (Worth Publishers, New York, 1975); Strachan and Read, Human Molecular Genetics, Second Edition (Wiley-Liss, New York, 1999); Eckstein, editor, Oligonucleotides and Analogs: A Practical Approach (Oxford University Press, New York, 1991); Gait, editor, Oligonucleotide Synthesis: A Practical Approach (IRL Press, Oxford, 1984)など。
本開示は、本開示の方法を実施するようにプログラムされているコンピューター制御システムを提供する。図3は、実質的に全体を通して説明されているように、生体分子の検出又は識別を補助するようにプログラムされているかそうでなければ構成されているコンピューターシステム301を示す。コンピューターシステム301は、例えば、光源、検出器(例えば、光検出器)、薬剤を放出するために利用される装置又は構成要素、反応条件(例えば、ハイブリダイゼーション、配列決定、酵素反応)を提供するのに利用される装置又は構成要素などの、生体分子の検出に利用される本開示の構成要素及び/又は装置の様々な態様を調整できる。コンピューターシステム301は、ユーザーの電子装置又は電子装置に対して遠隔に配置されたコンピューターシステムとすることができる。電子機器は携帯電子機器であり得る。
Claims (21)
- (a)および(b)を含む、生体試料中の生体分子のin situ検出又は同定に使用するための方法:
(a)(i)相対的な三次元(3D)空間関係を有する生体分子を含む、第1の体積の生体試料と、(ii)活性化剤と、(iii)付着部分とを用意すること;
(b)前記生体試料内に三次元マトリックスを形成すること、ここで、前記三次元マトリックスは、前記三次元マトリックスに前記付着部分を介して結合した前記生体分子を含み、前記三次元マトリックスは、前記生体試料中の前記生体分子の相対的な三次元空間的関係を保存し、前記三次元マトリックスを形成する間に、電磁気刺激、電気化学的刺激、又は熱刺激を含む刺激の印加により前記活性化剤が活性化され、前記第1の体積とは異なる第2の体積を有する前記生体試料又はその派生物を提供する。 - 前記(a)が前記活性化剤とは別個の1又は複数のポリマー前駆体を供給することをさらに含み、(b)において、前記三次元マトリックスは、前記1又は複数のポリマー前駆体を用いて形成される、請求項1に記載の方法。
- 前記生体試料が、細胞又はその派生物である、請求項1に記載の方法。
- 前記生体分子が、核酸分子、タンパク質分子又は小分子である、請求項1に記載の方法。
- 前記三次元マトリックスが、前記活性化剤の活性化により1.1~10倍に膨潤する、請求項1に記載の方法。
- 前記三次元マトリックスが、前記活性化剤の活性化により収縮する、請求項1に記載の方法。
- 前記刺激が電磁気刺激である、請求項1に記載の方法。
- 前記活性化剤がキレート官能性を含む、請求項1に記載の方法。
- 前記活性化剤がエチレンジアミン四酢酸(EDTA)を含む、請求項1に記載の方法。
- 前記生体分子の相対的な三次元空間的関係が、前記三次元マトリックスの膨潤又は収縮により保存される、請求項1に記載の方法。
- 前記第2の体積が、前記第1の体積より大きい、請求項1に記載の方法。
- 前記第2の体積が、前記第1の体積より小さい、請求項1に記載の方法。
- 前記付着部分が、反応性基を含むか、又は反応性基に作動可能に結合されている、請求項1に記載の方法。
- 前記付着部分が、アミン、チオール、アジド、アルキン、又はクリック反応性基を含む、請求項1に記載の方法。
- (b)に続いて、1又は複数の試薬を用いて前記生体分子中のある生体分子を検出又は同定することを更に含む、請求項1に記載の方法。
- 前記生体分子が、前記三次元マトリックス内で検出又は同定される、請求項15に記載の方法。
- 前記1又は複数の試薬がプローブを含む、請求項15に記載の方法。
- 前記プローブが核酸配列を含む、請求項17に記載の方法。
- 前記プローブの核酸配列を検出することを更に含むことによって、前記生体分子を検出又は同定する、請求項18に記載の方法。
- (i)~(iii)を含む、請求項1に記載の方法:
(i)刺激を印加することにより、前記活性化剤を活性化し、前記活性化剤の活性化により前記三次元マトリックスが、前記第2の体積が前記第1の体積より大きくなるように膨潤する、(ii)前記三次元マトリックスが膨潤した後、前記生体分子中のある生体分子を同定すること、及び(iii)前記三次元マトリックスが、第2の体積より小さい第3の体積に収縮するように追加の刺激を印加すること。 - 前記刺激が液体ではない、請求項1に記載の方法。
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Families Citing this family (161)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5893607B2 (ja) | 2010-04-05 | 2016-03-23 | プログノシス バイオサイエンシズ インコーポレイテッドPrognosys Biosciences,Inc. | 空間コード化生物学的アッセイ |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP2954065B1 (en) | 2013-02-08 | 2021-07-28 | 10X Genomics, Inc. | Partitioning and processing of analytes and other species |
WO2014210225A1 (en) | 2013-06-25 | 2014-12-31 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
AU2015243445B2 (en) | 2014-04-10 | 2020-05-28 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
CN106795553B (zh) | 2014-06-26 | 2021-06-04 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
KR20170073667A (ko) | 2014-10-29 | 2017-06-28 | 10엑스 제노믹스, 인크. | 표적화 핵산 서열 분석을 위한 방법 및 조성물 |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
CN112126675B (zh) | 2015-01-12 | 2022-09-09 | 10X基因组学有限公司 | 用于制备核酸测序文库的方法和系统以及用其制备的文库 |
EP3262188B1 (en) | 2015-02-24 | 2021-05-05 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
US10774370B2 (en) | 2015-12-04 | 2020-09-15 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
US11352667B2 (en) | 2016-06-21 | 2022-06-07 | 10X Genomics, Inc. | Nucleic acid sequencing |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
WO2018140966A1 (en) | 2017-01-30 | 2018-08-02 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
EP4230746A3 (en) | 2017-05-26 | 2023-11-01 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
US11597965B2 (en) | 2017-10-06 | 2023-03-07 | 10X Genomics, Inc. | RNA templated ligation |
EP3954782A1 (en) | 2017-11-15 | 2022-02-16 | 10X Genomics, Inc. | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
CN112262218A (zh) | 2018-04-06 | 2021-01-22 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
EP3836967A4 (en) | 2018-07-30 | 2022-06-15 | ReadCoor, LLC | METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS |
EP3844308A1 (en) | 2018-08-28 | 2021-07-07 | 10X Genomics, Inc. | Resolving spatial arrays |
US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
US20230323447A1 (en) | 2018-08-28 | 2023-10-12 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample |
WO2020096687A1 (en) * | 2018-09-05 | 2020-05-14 | Readcoor, Inc. | Methods and systems for therapeutic agent analysis |
WO2020076976A1 (en) * | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
CN114174531A (zh) | 2019-02-28 | 2022-03-11 | 10X基因组学有限公司 | 用空间条码化寡核苷酸阵列对生物分析物进行概况分析 |
WO2020190509A1 (en) | 2019-03-15 | 2020-09-24 | 10X Genomics, Inc. | Methods for using spatial arrays for single cell sequencing |
WO2020198071A1 (en) | 2019-03-22 | 2020-10-01 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
SG11202111878RA (en) | 2019-05-31 | 2021-11-29 | 10X Genomics Inc | Method of detecting target nucleic acid molecules |
WO2021021348A1 (en) * | 2019-07-31 | 2021-02-04 | The Regents Of The University Of California | In situ detection of signaling molecules by combination of chemical modification of endogenous proteins and proximity-ligation assay |
JP2022543560A (ja) * | 2019-08-05 | 2022-10-13 | ブリッドジーン バイオサイエンシズ インコーポレイテッド | 共有結合リガンドのプロテオームワイドな発見のための方法およびその組成物 |
CN117036248A (zh) | 2019-10-01 | 2023-11-10 | 10X基因组学有限公司 | 用于识别组织样品中的形态学模式的系统和方法 |
JP2023500485A (ja) * | 2019-10-28 | 2023-01-06 | クアンタム-エスアイ インコーポレイテッド | 単一細胞タンパク質及び核酸の配列決定の方法 |
US20210130881A1 (en) | 2019-11-06 | 2021-05-06 | 10X Genomics, Inc. | Imaging system hardware |
EP4055185A1 (en) | 2019-11-08 | 2022-09-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
WO2021097255A1 (en) | 2019-11-13 | 2021-05-20 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
AU2020388047A1 (en) | 2019-11-18 | 2022-06-23 | 10X Genomics, Inc. | Systems and methods for tissue classification |
CN117078725A (zh) | 2019-11-21 | 2023-11-17 | 10X基因组学有限公司 | 分析物的空间分析 |
WO2021102005A1 (en) | 2019-11-22 | 2021-05-27 | 10X Genomics, Inc. | Systems and methods for spatial analysis of analytes using fiducial alignment |
FI3891300T3 (fi) | 2019-12-23 | 2023-05-10 | 10X Genomics Inc | Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen |
CN115715329A (zh) | 2020-01-10 | 2023-02-24 | 10X基因组学有限公司 | 确定生物样品中靶核酸位置的方法 |
CN111189904A (zh) * | 2020-01-13 | 2020-05-22 | 深圳华大临床检验中心 | 一种检测生物样本中的蛋白质复合体的方法 |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
WO2021155063A1 (en) * | 2020-01-29 | 2021-08-05 | Readcoor, Llc | Compositions and methods for analyte detection |
US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
US20230081381A1 (en) | 2020-02-20 | 2023-03-16 | 10X Genomics, Inc. | METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS |
JP2023514749A (ja) | 2020-02-21 | 2023-04-07 | 10エックス ジェノミクス インコーポレイテッド | 統合型インサイチュ空間的アッセイのための方法および組成物 |
AU2021224760A1 (en) | 2020-02-21 | 2022-09-15 | 10X Genomics, Inc. | Capturing genetic targets using a hybridization approach |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
EP4242325A3 (en) | 2020-04-22 | 2023-10-04 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
US20230265491A1 (en) | 2020-05-04 | 2023-08-24 | 10X Genomics, Inc. | Spatial transcriptomic transfer modes |
US20230194469A1 (en) | 2020-05-19 | 2023-06-22 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
EP4143579A2 (en) * | 2020-05-20 | 2023-03-08 | Quantum-si Incorporated | Methods and compositions for protein sequencing |
EP4153776A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
WO2021237056A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Rna integrity analysis in a biological sample |
WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
AU2021283184A1 (en) | 2020-06-02 | 2023-01-05 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
EP4025692A2 (en) | 2020-06-02 | 2022-07-13 | 10X Genomics, Inc. | Nucleic acid library methods |
WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
WO2021252591A1 (en) | 2020-06-10 | 2021-12-16 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
WO2021252576A1 (en) | 2020-06-10 | 2021-12-16 | 10X Genomics, Inc. | Methods for spatial analysis using blocker oligonucleotides |
AU2021294334A1 (en) | 2020-06-25 | 2023-02-02 | 10X Genomics, Inc. | Spatial analysis of DNA methylation |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
EP4189110A1 (en) | 2020-07-31 | 2023-06-07 | 10X Genomics, Inc. | De-crosslinking compounds and methods of use for spatial analysis |
US11492662B2 (en) | 2020-08-06 | 2022-11-08 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
CN116547388A (zh) | 2020-09-15 | 2023-08-04 | 10X基因组学有限公司 | 从基材中释放延伸的捕获探针的方法及其应用 |
WO2022060953A1 (en) | 2020-09-16 | 2022-03-24 | 10X Genomics, Inc. | Methods of determining the location of an analyte in a biological sample using a plurality of wells |
EP4214678A2 (en) | 2020-09-18 | 2023-07-26 | 10X Genomics, Inc. | Sample handling apparatus and image registration methods |
WO2022061152A2 (en) | 2020-09-18 | 2022-03-24 | 10X Genomics, Inc. | Sample handling apparatus and fluid delivery methods |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
WO2022081643A2 (en) | 2020-10-13 | 2022-04-21 | 10X Genomics, Inc. | Compositions and methods for generating recombinant antigen binding molecules from single cells |
US20240018572A1 (en) | 2020-10-22 | 2024-01-18 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification |
EP4222283A1 (en) | 2020-11-06 | 2023-08-09 | 10X Genomics, Inc. | Compositions and methods for binding an analyte to a capture probe |
AU2021385065A1 (en) | 2020-11-18 | 2023-06-15 | 10X Genomics, Inc. | Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
US20220186300A1 (en) | 2020-12-11 | 2022-06-16 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
US20240042101A1 (en) * | 2020-12-21 | 2024-02-08 | University Of Washington | Photopatterned biomolecule immobilization to guide 3d cell fate in natural protein-based hydrogels |
US20240068017A1 (en) | 2020-12-30 | 2024-02-29 | 10X Genomics, Inc. | Methods for analyte capture determination |
WO2022147296A1 (en) | 2020-12-30 | 2022-07-07 | 10X Genomics, Inc. | Cleavage of capture probes for spatial analysis |
US20220228200A1 (en) | 2021-01-19 | 2022-07-21 | 10X Genomics, Inc. | Methods and compositions for internally controlled in situ assays |
US20240093290A1 (en) | 2021-01-29 | 2024-03-21 | 10X Genomics, Inc. | Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample |
WO2022187366A1 (en) | 2021-03-03 | 2022-09-09 | 10X Genomics, Inc. | Analyte detection in situ using nucleic acid origami |
EP4301870A1 (en) | 2021-03-18 | 2024-01-10 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
AU2022256031A1 (en) | 2021-04-05 | 2023-10-12 | 10X Genomics, Inc. | Recombinant ligase composition and uses thereof |
EP4305196A1 (en) | 2021-04-14 | 2024-01-17 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
WO2022226057A1 (en) | 2021-04-20 | 2022-10-27 | 10X Genomics, Inc. | Methods for assessing sample quality prior to spatial analysis using templated ligation |
WO2022236054A1 (en) | 2021-05-06 | 2022-11-10 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
WO2022256324A1 (en) | 2021-06-01 | 2022-12-08 | 10X Genomics, Inc. | Methods and compositions for analyte detection and probe resolution |
CN117751197A (zh) | 2021-06-02 | 2024-03-22 | 10X基因组学有限公司 | 使用不对称可环化探针的样品分析 |
WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
WO2022271820A1 (en) | 2021-06-22 | 2022-12-29 | 10X Genomics, Inc. | Spatial detection of sars-cov-2 using templated ligation |
WO2023283537A2 (en) * | 2021-07-07 | 2023-01-12 | Institute For Systems Biology | Cell analysis methods, compositions, and uses |
US20230026886A1 (en) | 2021-07-13 | 2023-01-26 | 10X Genomics, Inc. | Methods for preparing polymerized matrix with controllable thickness |
EP4352252A1 (en) | 2021-07-13 | 2024-04-17 | 10X Genomics, Inc. | Methods for spatial analysis using targeted probe silencing |
WO2023015192A1 (en) | 2021-08-03 | 2023-02-09 | 10X Genomics, Inc. | Nucleic acid concatemers and methods for stabilizing and/or compacting the same |
WO2023018799A1 (en) | 2021-08-12 | 2023-02-16 | 10X Genomics, Inc. | Methods, compositions and systems for identifying antigen-binding molecules |
US20230061542A1 (en) | 2021-08-16 | 2023-03-02 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
WO2023044071A1 (en) | 2021-09-17 | 2023-03-23 | 10X Genomics, Inc. | Systems and methods for image registration or alignment |
WO2023076345A1 (en) | 2021-10-26 | 2023-05-04 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna capture |
WO2023086824A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods for identification of antigen-binding molecules |
WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
US20230167495A1 (en) | 2021-11-30 | 2023-06-01 | 10X Genomics, Inc. | Systems and methods for identifying regions of aneuploidy in a tissue |
EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
US20230242974A1 (en) | 2021-12-27 | 2023-08-03 | 10X Genomics, Inc. | Methods and compositions for rolling circle amplification |
US20230279475A1 (en) | 2022-01-21 | 2023-09-07 | 10X Genomics, Inc. | Multiple readout signals for analyzing a sample |
WO2023150171A1 (en) | 2022-02-01 | 2023-08-10 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing analytes from glioblastoma samples |
WO2023150098A1 (en) | 2022-02-01 | 2023-08-10 | 10X Genomics, Inc. | Methods, kits, compositions, and systems for spatial analysis |
WO2023150163A1 (en) | 2022-02-01 | 2023-08-10 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing analytes from lymphatic tissue |
US20230306593A1 (en) | 2022-02-15 | 2023-09-28 | 10X Genomics, Inc. | Systems and methods for spatial analysis of analytes using fiducial alignment |
WO2023164570A1 (en) | 2022-02-23 | 2023-08-31 | Insitro, Inc. | Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations |
WO2023172670A2 (en) | 2022-03-11 | 2023-09-14 | 10X Genomics, Inc. | Sample handling apparatus and fluid delivery methods |
US20230323427A1 (en) | 2022-04-06 | 2023-10-12 | 10X Genomics, Inc. | Methods and compositions for multiplex cell analysis |
WO2023201235A2 (en) | 2022-04-12 | 2023-10-19 | 10X Genomics, Inc. | Compositions and methods for generating and characterizing recombinant antigen binding molecules |
WO2023215552A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Molecular barcode readers for analyte detection |
WO2023215612A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Analysis of antigen and antigen receptor interactions |
WO2023225519A1 (en) | 2022-05-17 | 2023-11-23 | 10X Genomics, Inc. | Modified transposons, compositions and uses thereof |
WO2023229988A1 (en) | 2022-05-23 | 2023-11-30 | 10X Genomics, Inc. | Tissue sample mold |
WO2023229982A2 (en) | 2022-05-24 | 2023-11-30 | 10X Genomics, Inc. | Porous structure confinement for convection suppression |
US20240035071A1 (en) | 2022-06-17 | 2024-02-01 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
WO2023250077A1 (en) | 2022-06-22 | 2023-12-28 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
WO2024002726A1 (en) | 2022-06-30 | 2024-01-04 | Resolve Biosciences Gmbh | High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample |
WO2024015862A1 (en) | 2022-07-13 | 2024-01-18 | 10X Genomics, Inc. | Methods for characterization of antigen-binding molecules from biological samples |
WO2024015578A1 (en) | 2022-07-15 | 2024-01-18 | 10X Genomics, Inc. | Methods for determining a location of a target nucleic acid in a biological sample |
US20240052404A1 (en) | 2022-08-05 | 2024-02-15 | 10X Genomics, Inc. | Systems and methods for immunofluorescence quantification |
WO2024036191A1 (en) | 2022-08-10 | 2024-02-15 | 10X Genomics, Inc. | Systems and methods for colocalization |
US20240101978A1 (en) | 2022-08-12 | 2024-03-28 | 10X Genomics, Inc. | Puma1 polymerases and uses thereof |
WO2024035844A1 (en) | 2022-08-12 | 2024-02-15 | 10X Genomics, Inc. | Methods for reducing capture of analytes |
WO2024040060A1 (en) | 2022-08-16 | 2024-02-22 | 10X Genomics, Inc. | Ap50 polymerases and uses thereof |
WO2024044703A1 (en) | 2022-08-24 | 2024-02-29 | 10X Genomics, Inc. | Compositions and methods for antigenic epitope mapping in biological samples |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015127183A2 (en) | 2014-02-21 | 2015-08-27 | Massachusetts Institute Of Technology | Expansion microscopy |
US20160024555A1 (en) | 2013-03-12 | 2016-01-28 | President And Fellows Of Harvard College | Method for Generating A Three-Dimensional Nucleic Acid Containing Matrix |
Family Cites Families (170)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4123610A (en) | 1977-03-09 | 1978-10-31 | The United States Government | Nucleic acid crosslinking agent and affinity inactivation of nucleic acids therewith |
US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
US4981985A (en) * | 1987-10-20 | 1991-01-01 | Trustees Of The University Of Pennsylvania | Synthesis of photolabile chelators for multivalent cations |
US4886741A (en) | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
US4844617A (en) | 1988-01-20 | 1989-07-04 | Tencor Instruments | Confocal measuring microscope with automatic focusing |
US5151189A (en) | 1990-09-17 | 1992-09-29 | Gelman Sciences, Inc. | Cationic charge modified microporous membrane |
JPH04268359A (ja) * | 1991-02-22 | 1992-09-24 | Taiho Yakuhin Kogyo Kk | 刺激応答性ヒドロゲル及びその製造方法 |
JP3455217B2 (ja) | 1992-02-13 | 2003-10-14 | バイオ−メトリック システムズ インコーポレイテッド | 架橋マトリックス中の化学種の固定 |
US5594235A (en) | 1993-06-17 | 1997-01-14 | Ultrapointe Corporation | Automated surface acquisition for a confocal microscope |
US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
US5830708A (en) | 1995-06-06 | 1998-11-03 | Advanced Tissue Sciences, Inc. | Methods for production of a naturally secreted extracellular matrix |
US6284284B1 (en) | 1995-06-06 | 2001-09-04 | Advanced Tissue Sciences, Inc. | Compositions and methods for production and use of an injectable naturally secreted extracellular matrix |
EP0923650B1 (en) | 1996-06-06 | 2007-03-07 | Solexa, Inc | Sequencing by ligation of encoded adaptors |
JP4532608B2 (ja) | 1997-02-07 | 2010-08-25 | オリンパス株式会社 | 走査型プローブ顕微鏡を用いた核酸検出方法 |
US5948617A (en) | 1997-06-13 | 1999-09-07 | Biospeparations, Inc. | Methods of in situ hybridization |
US6083726A (en) | 1998-02-03 | 2000-07-04 | Lucent Technologies, Inc. | Methods for polynucleotide synthesis and articles for polynucleotide hybridization |
US6068979A (en) | 1998-04-22 | 2000-05-30 | Lumigen, Inc. | Simplified sequential chemiluminescent detection |
EP1090293B2 (en) | 1998-06-24 | 2019-01-23 | Illumina, Inc. | Decoding of array sensors with microspheres |
DE69931497T2 (de) | 1998-08-07 | 2007-05-03 | Cellay LLC, Cambridge | Gel mikrotropfen für die genetische analyse |
EP1163052B1 (en) | 1999-02-23 | 2010-06-02 | Caliper Life Sciences, Inc. | Manipulation of microparticles in microfluidic systems |
WO2000065094A2 (en) | 1999-04-22 | 2000-11-02 | The Albert Einstein College Of Medicine Of Yeshiva University | Assay of gene expression patterns by multi-fluor fish |
US20020015952A1 (en) | 1999-07-30 | 2002-02-07 | Anderson Norman G. | Microarrays and their manufacture by slicing |
CA2386151A1 (en) * | 1999-10-12 | 2001-04-19 | Marc Madou | Reactive polymeric valve, dispensing devices and methods using same |
WO2001037266A1 (en) | 1999-11-17 | 2001-05-25 | The Research Foundation Of State University Of New York | Three dimensional data storage device and method for reading |
US7115738B2 (en) | 2000-03-14 | 2006-10-03 | Active Motif | Hydroxyproline/phosphono oligonucleotide analogues, methods of synthesis and methods of use |
US7033758B2 (en) | 2000-06-02 | 2006-04-25 | Bayer Corporation | Highly sensitive gene detection and localization using in situ branched-DNA hybridization |
US7613571B2 (en) | 2000-07-28 | 2009-11-03 | Doyle Michael D | Method and system for the multidimensional morphological reconstruction of genome expression activity |
US6797152B2 (en) | 2000-07-31 | 2004-09-28 | California Institute Of Technology | Sensors and sensing methods for detecting analytes based on changes in pKa of a sensing polymer |
CA2428757A1 (en) | 2000-11-15 | 2002-07-18 | Roche Diagnostics Corporation | Methods and reagents for identifying rare fetal cells in the maternal circulation |
ATE447043T1 (de) | 2000-12-14 | 2009-11-15 | Gen Probe Inc | Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden |
DK1370690T3 (da) | 2001-03-16 | 2012-07-09 | Kalim Mir | Arrays og fremgangsmåder til anvendelse heraf |
US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
US20060248349A1 (en) | 2001-09-24 | 2006-11-02 | Rathjen Alicemarie G | Method for preparing and using personal and genetic profiles |
WO2003031591A2 (en) | 2001-10-10 | 2003-04-17 | Superarray Bioscience Corporation | Detecting targets by unique identifier nucleotide tags |
GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
CA2462819A1 (en) | 2001-11-23 | 2003-05-30 | Simon Fredriksson | Method and kit for proximity probing with multivalent proximity probes |
US20090246879A1 (en) | 2002-12-20 | 2009-10-01 | Callida Genomics | Materials and Methods Relating to Nano-Tags and Nano-Brands |
AU2004254552B2 (en) | 2003-01-29 | 2008-04-24 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
AU2004214891B2 (en) | 2003-02-26 | 2010-01-07 | Complete Genomics, Inc. | Random array DNA analysis by hybridization |
US7385043B1 (en) | 2003-04-30 | 2008-06-10 | The Public Health Research Institute Of The City Of New York, Inc. | Homogeneous multiplex screening assays and kits |
ES2594054T3 (es) | 2003-05-20 | 2016-12-15 | Lucid, Inc. | Microscopio confocal para la formación de imágenes de secciones seleccionadas del cuerpo de un paciente |
WO2005047543A2 (en) | 2003-06-10 | 2005-05-26 | Applera Corporation | Ligation assay |
CN1580283A (zh) | 2003-08-13 | 2005-02-16 | 清华大学 | 一种检测核酸分子的方法 |
US7193054B2 (en) | 2003-08-26 | 2007-03-20 | University Of Rochester | Nanofabrication using actin filaments |
US20050064435A1 (en) | 2003-09-24 | 2005-03-24 | Xing Su | Programmable molecular barcodes |
US20080050718A1 (en) | 2003-11-14 | 2008-02-28 | Gesteland Raymond F | Methods, Articles, and Compositions for Identifying Oligonucleotides |
US7381529B2 (en) | 2003-12-31 | 2008-06-03 | Intel Corporation | Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes |
CA2557841A1 (en) | 2004-02-27 | 2005-09-09 | President And Fellows Of Harvard College | Polony fluorescent in situ sequencing beads |
WO2005085850A1 (en) | 2004-02-27 | 2005-09-15 | Tianxin Wang | Methods for multiplexed analyte detection |
US7427479B2 (en) | 2004-04-30 | 2008-09-23 | Applera Corporation | Methods and kits for identifying target nucleotides in mixed populations |
US20060024711A1 (en) | 2004-07-02 | 2006-02-02 | Helicos Biosciences Corporation | Methods for nucleic acid amplification and sequence determination |
US20060046311A1 (en) * | 2004-08-26 | 2006-03-02 | Intel Corporation | Biomolecule analysis using Raman surface scanning |
US7253947B2 (en) | 2004-10-07 | 2007-08-07 | New York University | Portable automated confocal microscope |
EP1844162B1 (en) | 2005-02-01 | 2014-10-15 | Applied Biosystems, LLC | Method for identifying a sequence in a polynucleotide |
US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
ITRM20050068A1 (it) | 2005-02-17 | 2006-08-18 | Istituto Naz Per Le Malattie I | Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine. |
US20060234261A1 (en) | 2005-03-08 | 2006-10-19 | Pierce Niles A | Colorimetric readout of hybridization chain reaction |
US7727721B2 (en) | 2005-03-08 | 2010-06-01 | California Institute Of Technology | Hybridization chain reaction amplification for in situ imaging |
JP2008537739A (ja) | 2005-03-21 | 2008-09-25 | ザ トラスティース オブ コロンビア ユニバーシティ イン ザ シティ オブ ニューヨーク | 新規神経疼痛経路 |
US20070020650A1 (en) | 2005-04-01 | 2007-01-25 | Avak Kahvejian | Methods for detecting proteins |
CA2603869A1 (en) | 2005-04-08 | 2007-08-02 | Mycosol, Inc. | Stilbazium research assays |
US11561951B2 (en) | 2005-05-16 | 2023-01-24 | Panvia Future Technologies, Inc. | Multidimensional associative memory and data searching |
EP1910537A1 (en) | 2005-06-06 | 2008-04-16 | 454 Life Sciences Corporation | Paired end sequencing |
US7842793B2 (en) | 2005-06-14 | 2010-11-30 | The California Institute Of Technology | Methods of making nucleic acid nanostructures |
SG162795A1 (en) | 2005-06-15 | 2010-07-29 | Callida Genomics Inc | Single molecule arrays for genetic and chemical analysis |
ES2565670T3 (es) | 2005-06-20 | 2016-04-06 | Advanced Cell Diagnostics, Inc. | Métodos de detección de ácidos nucleicos en células individuales y de identificación de células raras en grandes poblaciones de células heterogéneas |
US9017992B2 (en) | 2005-07-01 | 2015-04-28 | Dako Denmark A/S | In situ hybridization detection method |
US8486621B2 (en) | 2005-08-11 | 2013-07-16 | Cornell Research Foundation, Inc. | Nucleic acid-based matrixes |
CN1959384A (zh) | 2005-11-01 | 2007-05-09 | 河南省生物工程技术研究中心 | 一种三维网格检测技术 |
EP1963500B1 (en) | 2005-12-23 | 2013-02-13 | Nanostring Technologies, Inc. | Compositions comprising oriented, immobilized macromolecules and methods for their preparation |
CA2640385C (en) | 2005-12-23 | 2014-07-15 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
JP4931257B2 (ja) * | 2006-01-19 | 2012-05-16 | ザ リサーチ ファウンデーション オブ ステイト ユニバーシティ オブ ニューヨーク | コード化ビーズおよび生物学的分子の検出および同定のための方法およびデバイス |
US7864996B2 (en) | 2006-02-17 | 2011-01-04 | Lucid, Inc. | System for macroscopic and confocal imaging of tissue |
WO2007106402A2 (en) | 2006-03-10 | 2007-09-20 | President And Fellows Of Harvard College | Methods and apparatus for near field irradiation |
GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
US20070231823A1 (en) | 2006-03-23 | 2007-10-04 | Mckernan Kevin J | Directed enrichment of genomic DNA for high-throughput sequencing |
US8975216B2 (en) | 2006-03-30 | 2015-03-10 | Pacific Biosciences Of California | Articles having localized molecules disposed thereon and methods of producing same |
WO2007123744A2 (en) | 2006-03-31 | 2007-11-01 | Solexa, Inc. | Systems and devices for sequence by synthesis analysis |
AU2007237909A1 (en) | 2006-04-19 | 2007-10-25 | Applied Biosystems, Llc. | Reagents, methods, and libraries for gel-free bead-based sequencing |
JP5081232B2 (ja) | 2006-05-22 | 2012-11-28 | ナノストリング テクノロジーズ, インコーポレイテッド | ナノレポーターを分析するためのシステムおよび方法 |
US7964347B2 (en) | 2006-06-15 | 2011-06-21 | Krassen Dimitrov | Labels for electronic detection of individual molecules and methods for their detection |
US20080038163A1 (en) | 2006-06-23 | 2008-02-14 | Applera Corporation | Systems and Methods for Cooling in Biological Analysis Instruments |
US7745129B1 (en) | 2006-07-12 | 2010-06-29 | Kenneth David Schatz | Methods for sequencing of a necleic acid |
US20100009868A1 (en) | 2006-09-11 | 2010-01-14 | The Arizona Board of Regents a body corporate acti ng for and behalf of Arizona State university | Self-Assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing |
US20090208965A1 (en) | 2006-10-25 | 2009-08-20 | Ikonisys, Inc. | Automated method for detecting cancers and high grade hyperplasias |
WO2008055915A2 (en) | 2006-11-06 | 2008-05-15 | Clondiag Gmbh | Device and process for assays using binding members |
US9201063B2 (en) | 2006-11-16 | 2015-12-01 | General Electric Company | Sequential analysis of biological samples |
US7883869B2 (en) | 2006-12-01 | 2011-02-08 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
US20080269068A1 (en) | 2007-02-06 | 2008-10-30 | President And Fellows Of Harvard College | Multiplex decoding of sequence tags in barcodes |
US8145677B2 (en) | 2007-03-27 | 2012-03-27 | Faleh Jassem Al-Shameri | Automated generation of metadata for mining image and text data |
CA2687292C (en) | 2007-04-10 | 2017-07-04 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
WO2008144562A1 (en) | 2007-05-16 | 2008-11-27 | California Institute Of Technology | A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways |
US7939259B2 (en) | 2007-06-19 | 2011-05-10 | Stratos Genomics, Inc. | High throughput nucleic acid sequencing by expansion |
EP2185725A4 (en) | 2007-08-02 | 2011-01-12 | Biodesic | COMPOSITIONS AND METHODS FOR IDENTIFYING AND QUANTIFYING ANALYTES |
EP2212434A1 (en) | 2007-10-01 | 2010-08-04 | Applied Biosystems Inc. | Chase ligation sequencing |
EA201000427A1 (ru) | 2007-10-04 | 2010-10-29 | Хэлсион Молекулар | Секвенирование нуклеиново-кислотных полимеров с использованием электронной микроскопии |
US20090139311A1 (en) | 2007-10-05 | 2009-06-04 | Applied Biosystems Inc. | Biological Analysis Systems, Devices, and Methods |
WO2009065093A2 (en) | 2007-11-17 | 2009-05-22 | University Of Medicine And Dentistry Of New Jersey | Use of stem cells for wound healing |
CN101586150B (zh) | 2008-05-23 | 2016-09-28 | 陕西佰美基因股份有限公司 | 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途 |
US20120040397A1 (en) | 2008-08-05 | 2012-02-16 | Dan Luo | Photo-Crosslinked Nucleic Acid Hydrogels |
WO2010019826A1 (en) | 2008-08-14 | 2010-02-18 | Nanostring Technologies, Inc | Stable nanoreporters |
AU2009291752B2 (en) | 2008-09-10 | 2015-12-17 | Rutgers, The State University Of New Jersey | Imaging individual mRNA molecules using multiple singly labeled probes |
US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
US20100087325A1 (en) | 2008-10-07 | 2010-04-08 | Illumina, Inc. | Biological sample temperature control system and method |
US8309306B2 (en) | 2008-11-12 | 2012-11-13 | Nodality, Inc. | Detection composition |
US8748094B2 (en) | 2008-12-19 | 2014-06-10 | President And Fellows Of Harvard College | Particle-assisted nucleic acid sequencing |
JP4528345B2 (ja) | 2009-01-28 | 2010-08-18 | シャープ株式会社 | 動画再生装置、動画再生方法、動画再生方法をコンピュータで実現するためのプログラム及びそのプログラムを記録した記録媒体 |
JP5277082B2 (ja) | 2009-06-15 | 2013-08-28 | 株式会社日立ハイテクノロジーズ | 蛍光分析方法 |
FR2948475A1 (fr) | 2009-07-24 | 2011-01-28 | Bionext | Procede de caracterisation d'objets tridimensionnels |
US8431151B2 (en) | 2009-08-06 | 2013-04-30 | Syracuse University | Antimicrobial nanostructured hydrogel web containing silver |
WO2011047087A2 (en) | 2009-10-13 | 2011-04-21 | Nanostring Technologies, Inc. | Protein detection via nanoreporters |
AU2010315303B2 (en) | 2009-11-03 | 2015-08-06 | Htg Molecular Diagnostics, Inc. | Quantitative Nuclease Protection Sequencing (qNPS) |
WO2011075516A2 (en) * | 2009-12-18 | 2011-06-23 | President And Fellows Of Harvard College | Active scaffolds for on-demand drug and cell delivery |
US8632976B2 (en) | 2010-01-19 | 2014-01-21 | Agdia | Amplification of TRP1 for specific detection of Phytophthora ramorum |
JP5914362B2 (ja) | 2010-02-11 | 2016-05-11 | ナノストリング テクノロジーズ, インコーポレイテッド | 低分子rna分子の検出のための組成物および方法 |
US20110257031A1 (en) | 2010-02-12 | 2011-10-20 | Life Technologies Corporation | Nucleic acid, biomolecule and polymer identifier codes |
US10267808B2 (en) | 2010-03-08 | 2019-04-23 | California Institute Of Technology | Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells |
GB201004292D0 (en) | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
JP5893607B2 (ja) | 2010-04-05 | 2016-03-23 | プログノシス バイオサイエンシズ インコーポレイテッドPrognosys Biosciences,Inc. | 空間コード化生物学的アッセイ |
US20130059741A1 (en) | 2010-05-13 | 2013-03-07 | Illumina, Inc. | Binding assays for markers |
KR101862756B1 (ko) | 2010-07-09 | 2018-05-30 | 세르겐티스 비.브이. | 관심있는 3-d 게놈 영역 서열화 전략 |
US8787651B2 (en) | 2010-09-28 | 2014-07-22 | Flagship Biosciences, LLC | Methods for feature analysis on consecutive tissue sections |
ES2562821T3 (es) | 2010-10-21 | 2016-03-08 | Advanced Cell Diagnostics, Inc. | Método ultrasensible para la detección in situ de ácidos nucleicos |
WO2012056440A1 (en) | 2010-10-28 | 2012-05-03 | Nanodoc Ltd. | COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA |
US10024796B2 (en) | 2010-10-29 | 2018-07-17 | President And Fellows Of Harvard College | Nucleic acid nanostructure barcode probes |
EP2633081B1 (en) | 2010-10-29 | 2017-01-11 | Olink Bioscience AB | Proximity ligation technology for western blot applications |
EP2640849B1 (en) | 2010-11-16 | 2016-04-06 | Nabsys 2.0 LLC | Methods for sequencing a biomolecule by detecting relative positions of hybridized probes |
WO2012110899A2 (en) | 2011-02-15 | 2012-08-23 | Bernitz Mats Nilsson | Method for localizedin situdetection of mrna |
JP5687514B2 (ja) | 2011-02-17 | 2015-03-18 | 株式会社日立ハイテクノロジーズ | 核酸配列解析装置 |
EP2500436B1 (en) | 2011-03-17 | 2016-05-25 | Institut Pasteur | Method, probe and kit for DNA in situ hybridation and use thereof |
US20120252686A1 (en) | 2011-03-31 | 2012-10-04 | Good Start Genetics | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US8946389B2 (en) | 2011-04-25 | 2015-02-03 | University Of Washington | Compositions and methods for multiplex biomarker profiling |
WO2012150035A1 (de) | 2011-05-04 | 2012-11-08 | Genovoxx Gmbh | Nukleosid-triphosphat-konjugate und methoden zu deren anwendung |
GB201108678D0 (en) | 2011-05-24 | 2011-07-06 | Olink Ab | Multiplexed proximity ligation assay |
US9617598B2 (en) | 2011-05-27 | 2017-04-11 | President And Fellows Of Harvard College | Methods of amplifying whole genome of a single cell |
PL222511B1 (pl) | 2011-06-08 | 2016-08-31 | Międzynarodowy Inst Biologii Molekularnej I Komórkowej W Warszawie | Endorybonukleazy dsRNA |
US10501791B2 (en) | 2011-10-14 | 2019-12-10 | President And Fellows Of Harvard College | Sequencing by structure assembly |
US11021737B2 (en) | 2011-12-22 | 2021-06-01 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
US10227639B2 (en) | 2011-12-22 | 2019-03-12 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
ES2663234T3 (es) | 2012-02-27 | 2018-04-11 | Cellular Research, Inc | Composiciones y kits para recuento molecular |
US11225683B2 (en) | 2012-07-20 | 2022-01-18 | Lsi Medience Corporation | Photocoupling method using probe containing photoresponsive nucleic acids |
EP2883030B1 (en) | 2012-08-09 | 2022-08-31 | The Board of Trustees of the Leland Stanford Junior University | Methods and compositions for preparing biological specimens for microscopic analysis |
WO2014028538A2 (en) | 2012-08-13 | 2014-02-20 | William Marsh Rice University | Multiplexed in situ molecular analyses and programmable molecular probes for regulated signal amplification |
BR112015003464B1 (pt) | 2012-08-15 | 2022-01-18 | Lucid, Inc | Sistemas e processos para formação de imagens de tecido |
CN102908119A (zh) | 2012-09-26 | 2013-02-06 | 温州医学院眼视光研究院 | 一种共焦扫描成像系统及其像差控制方法 |
US9411930B2 (en) | 2013-02-01 | 2016-08-09 | The Regents Of The University Of California | Methods for genome assembly and haplotype phasing |
US9330295B2 (en) | 2013-03-15 | 2016-05-03 | Brown University | Spatial sequencing/gene expression camera |
US10510435B2 (en) | 2013-04-30 | 2019-12-17 | California Institute Of Technology | Error correction of multiplex imaging analysis by sequential hybridization |
WO2014182528A2 (en) | 2013-04-30 | 2014-11-13 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding |
US10136900B2 (en) * | 2013-05-26 | 2018-11-27 | M.A.S. Med Global Ltd | Pneumatic tourniquet |
WO2015058052A1 (en) | 2013-10-18 | 2015-04-23 | The Broad Institute Inc. | Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing |
GB201401885D0 (en) | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
US10179932B2 (en) | 2014-07-11 | 2019-01-15 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
KR102298387B1 (ko) | 2014-11-21 | 2021-09-07 | 나노스트링 테크놀로지스, 인크. | 무효소 및 무증폭 시퀀싱 |
EP3227830B8 (en) | 2014-12-03 | 2020-07-01 | Ventana Medical Systems, Inc. | Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution |
WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
US9607375B2 (en) | 2015-06-03 | 2017-03-28 | Eileen B. Gallagher | Biological data annotation and visualization |
EP3332034A4 (en) | 2015-08-06 | 2019-01-02 | Arc Bio, LLC | Systems and methods for genomic analysis |
WO2017079382A1 (en) | 2015-11-03 | 2017-05-11 | President And Fellows Of Harvard College | Systems and methods for processing spatially related sequence data received from a sequencing device |
MX2018005611A (es) | 2015-11-03 | 2018-11-09 | Harvard College | Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico. |
US10386300B2 (en) | 2015-12-21 | 2019-08-20 | Verily Life Sciences Llc | Spectrally and spatially multiplexed fluorescent probes for in situ cell labeling |
US10844426B2 (en) | 2016-03-17 | 2020-11-24 | President And Fellows Of Harvard College | Methods for detecting and identifying genomic nucleic acids |
CN109415761B (zh) | 2016-04-25 | 2022-09-20 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
AU2017291727B2 (en) | 2016-07-05 | 2021-07-08 | California Institute Of Technology | Fractional initiator hybridization chain reaction |
CA3031586A1 (en) | 2016-07-27 | 2018-02-01 | The Board Of Trustees Of The Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
WO2018183876A1 (en) | 2017-03-31 | 2018-10-04 | Ultivue, Inc. | Dna-antigen exchange and amplification |
-
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- 2017-08-31 CN CN201780067534.8A patent/CN109923216A/zh active Pending
- 2017-08-31 GB GB1904335.5A patent/GB2569252A/en not_active Withdrawn
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160024555A1 (en) | 2013-03-12 | 2016-01-28 | President And Fellows Of Harvard College | Method for Generating A Three-Dimensional Nucleic Acid Containing Matrix |
WO2015127183A2 (en) | 2014-02-21 | 2015-08-27 | Massachusetts Institute Of Technology | Expansion microscopy |
Non-Patent Citations (3)
Title |
---|
Nature methods,2016年,Vol.13, No.8,p.679-684 |
Nature methods,2016年06月,Vol.13, No.6,p.485-488 |
Science,2015年,Vol.347, No.6221,p.543-548 |
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