JP6931665B2 - パルス決定器及び塩基決定器 - Google Patents
パルス決定器及び塩基決定器 Download PDFInfo
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- JP6931665B2 JP6931665B2 JP2018562554A JP2018562554A JP6931665B2 JP 6931665 B2 JP6931665 B2 JP 6931665B2 JP 2018562554 A JP2018562554 A JP 2018562554A JP 2018562554 A JP2018562554 A JP 2018562554A JP 6931665 B2 JP6931665 B2 JP 6931665B2
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- G01N21/645—Specially adapted constructive features of fluorimeters
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Burglar Alarm Systems (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662343997P | 2016-06-01 | 2016-06-01 | |
| US62/343,997 | 2016-06-01 | ||
| PCT/US2017/035420 WO2017210413A1 (en) | 2016-06-01 | 2017-06-01 | Pulse caller and base caller |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019529865A JP2019529865A (ja) | 2019-10-17 |
| JP2019529865A5 JP2019529865A5 (enExample) | 2020-07-09 |
| JP6931665B2 true JP6931665B2 (ja) | 2021-09-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018562554A Active JP6931665B2 (ja) | 2016-06-01 | 2017-06-01 | パルス決定器及び塩基決定器 |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US10711299B2 (enExample) |
| EP (2) | EP4009330A1 (enExample) |
| JP (1) | JP6931665B2 (enExample) |
| KR (1) | KR102425257B1 (enExample) |
| CN (1) | CN109313925B (enExample) |
| AU (1) | AU2017274412B2 (enExample) |
| BR (1) | BR112018074572B1 (enExample) |
| CA (1) | CA3026061A1 (enExample) |
| MX (1) | MX2018014856A (enExample) |
| TW (1) | TWI755400B (enExample) |
| WO (1) | WO2017210413A1 (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3194935B1 (en) | 2014-08-08 | 2018-10-31 | Quantum-si Incorporated | Integrated device for temporal binning of received photons |
| KR20180111999A (ko) | 2016-02-17 | 2018-10-11 | 테서렉트 헬스, 인코포레이티드 | 수명 촬상 및 검출 응용을 위한 센서 및 디바이스 |
| KR102627237B1 (ko) * | 2016-06-01 | 2024-01-23 | 퀀텀-에스아이 인코포레이티드 | 분자들을 검출 및 분석하기 위한 통합 디바이스 |
| CA3046680A1 (en) | 2016-12-19 | 2018-06-28 | Quantum-Si Incorporated | Loading molecules into sample wells for analysis |
| CN110168732B (zh) | 2016-12-22 | 2024-03-08 | 宽腾矽公司 | 具有直接合并像素的整合式光电侦测器 |
| US11120104B2 (en) | 2017-03-01 | 2021-09-14 | Stmicroelectronics (Research & Development) Limited | Method and apparatus for processing a histogram output from a detector sensor |
| WO2018204810A1 (en) | 2017-05-05 | 2018-11-08 | Quantum-Si Incorporated | Substrates having modified surface reactivity and antifouling properties in biological reactions |
| WO2019136376A1 (en) | 2018-01-08 | 2019-07-11 | Illumina, Inc. | High-throughput sequencing with semiconductor-based detection |
| NL2020621B1 (en) | 2018-01-08 | 2019-07-15 | Illumina Inc | Multiplexing of an active sensor detector using structured illumination |
| AU2019205496B2 (en) | 2018-01-08 | 2021-08-19 | Illumina, Inc. | High-throughput sequencing with semiconductor-based detection |
| MX2020007904A (es) | 2018-01-26 | 2020-09-07 | Quantum Si Inc | Llamado de pulso y base habilitado por maquina de aprendizaje para dispositivos de secuenciacion. |
| CA3108295A1 (en) | 2018-06-22 | 2019-12-26 | Quantum-Si Incorporated | Integrated photodetector with charge storage bin of varied detection time |
| EP3844486A1 (en) * | 2018-08-29 | 2021-07-07 | Quantum-Si Incorporated | System and methods for detecting lifetime using photon counting photodetectors |
| US11783917B2 (en) | 2019-03-21 | 2023-10-10 | Illumina, Inc. | Artificial intelligence-based base calling |
| US11210554B2 (en) | 2019-03-21 | 2021-12-28 | Illumina, Inc. | Artificial intelligence-based generation of sequencing metadata |
| US11423306B2 (en) | 2019-05-16 | 2022-08-23 | Illumina, Inc. | Systems and devices for characterization and performance analysis of pixel-based sequencing |
| US11593649B2 (en) | 2019-05-16 | 2023-02-28 | Illumina, Inc. | Base calling using convolutions |
| AU2020364058A1 (en) | 2019-10-11 | 2022-05-26 | Quantum-Si Incorporated | Surface modification in the vapor phase |
| US11869917B2 (en) | 2020-01-14 | 2024-01-09 | Quantum-Si Incorporated | Integrated sensor for lifetime characterization |
| EP4088319A1 (en) | 2020-01-14 | 2022-11-16 | Quantum-si Incorporated | Sensor for lifetime plus spectral characterization |
| US20210265016A1 (en) | 2020-02-20 | 2021-08-26 | Illumina, Inc. | Data Compression for Artificial Intelligence-Based Base Calling |
| US12354008B2 (en) | 2020-02-20 | 2025-07-08 | Illumina, Inc. | Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller |
| US11749380B2 (en) | 2020-02-20 | 2023-09-05 | Illumina, Inc. | Artificial intelligence-based many-to-many base calling |
| US11719639B2 (en) * | 2020-03-02 | 2023-08-08 | Quantum-Si Incorporated | Integrated sensor for multi-dimensional signal analysis |
| WO2021207400A2 (en) | 2020-04-08 | 2021-10-14 | Quantum-Si Incorporated | Integrated sensor with reduced skew |
| US20220065785A1 (en) * | 2020-07-23 | 2022-03-03 | Quantum-Si Incorporated | System and method for detecting change points |
| CA3199745A1 (en) * | 2020-10-27 | 2022-05-05 | Quantum-Si Incorporated | Calibration of single-molecule detection system |
| US12217829B2 (en) | 2021-04-15 | 2025-02-04 | Illumina, Inc. | Artificial intelligence-based analysis of protein three-dimensional (3D) structures |
| US20220336057A1 (en) | 2021-04-15 | 2022-10-20 | Illumina, Inc. | Efficient voxelization for deep learning |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2155186A1 (en) * | 1993-02-01 | 1994-08-18 | Kevin M. Ulmer | Methods and apparatus for dna sequencing |
| DE19744490A1 (de) * | 1997-10-08 | 1999-04-15 | Temp Rite International Gmbh | Trinkwasseraufbereitungsanlage |
| JP4566299B2 (ja) * | 1998-04-09 | 2010-10-20 | シスメックス株式会社 | 赤芽球の分類計数方法 |
| AU5479599A (en) | 1998-08-11 | 2000-03-06 | Caliper Technologies Corporation | Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes |
| DE19844931C1 (de) * | 1998-09-30 | 2000-06-15 | Stefan Seeger | Verfahren zur DNS- oder RNS-Sequenzierung |
| US7056661B2 (en) * | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| GB2382648B (en) * | 2001-12-11 | 2003-11-12 | Amersham Pharm Biotech Uk Ltd | System and method for time correlated multi-photon counting measurements |
| WO2005120204A2 (en) * | 2004-06-07 | 2005-12-22 | The Regents Of The University Of California | Method for single molecule fluorescence analysis |
| CA2689626C (en) | 2007-06-06 | 2016-10-25 | Pacific Biosciences Of California, Inc. | Methods and processes for calling bases in sequence by incorporation methods |
| JP5643650B2 (ja) | 2007-11-21 | 2014-12-17 | コスモシド・インコーポレーテッド | ゲノム同定システム |
| WO2009091847A2 (en) * | 2008-01-14 | 2009-07-23 | Life Technologies Corporation | Compositions, methods and systems for single molecule sequencing |
| ES2573092T3 (es) * | 2009-07-10 | 2016-06-06 | Perkinelmer Health Sciences, Inc. | Detección de repeticiones de multinucleótido |
| US9670243B2 (en) * | 2010-06-02 | 2017-06-06 | Industrial Technology Research Institute | Compositions and methods for sequencing nucleic acids |
| US20140235474A1 (en) * | 2011-06-24 | 2014-08-21 | Sequenom, Inc. | Methods and processes for non invasive assessment of a genetic variation |
| US9145623B2 (en) * | 2011-07-20 | 2015-09-29 | Thermo Fisher Scientific Oy | Transposon nucleic acids comprising a calibration sequence for DNA sequencing |
| JP5790242B2 (ja) * | 2011-07-25 | 2015-10-07 | 富士ゼロックス株式会社 | 故障予測装置及びプログラム |
| AU2013240166A1 (en) * | 2012-03-30 | 2014-10-30 | Pacific Biosciences Of California, Inc. | Methods and composition for sequencing modified nucleic acids |
| WO2013191775A2 (en) * | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| AU2014268417A1 (en) * | 2013-05-23 | 2015-11-26 | Iphenotype Llc | Methods and systems for assisting persons, product providers and/or service providers |
| MX392471B (es) * | 2013-11-17 | 2025-03-24 | Quantum Si Inc | Sistema optico y chip de ensayo para sondear, detectar y analizar moleculas |
| WO2015095066A1 (en) | 2013-12-16 | 2015-06-25 | Complete Genomics, Inc. | Basecaller for dna sequencing using machine learning |
| EP3143537B1 (en) | 2014-05-12 | 2023-03-01 | Roche Diagnostics GmbH | Rare variant calls in ultra-deep sequencing |
| EP3194935B1 (en) * | 2014-08-08 | 2018-10-31 | Quantum-si Incorporated | Integrated device for temporal binning of received photons |
| KR102472061B1 (ko) | 2014-08-08 | 2022-11-29 | 퀀텀-에스아이 인코포레이티드 | 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩 |
| BR112017002501B1 (pt) | 2014-08-08 | 2022-06-07 | Quantum-Si Incorporated | Dispositivos integrados, sistemas, método de detecção de presença de molécula em amostra, método de formação de dispositivo integrado e método de análise de espécime |
| US10185803B2 (en) | 2015-06-15 | 2019-01-22 | Deep Genomics Incorporated | Systems and methods for classifying, prioritizing and interpreting genetic variants and therapies using a deep neural network |
| US9922285B1 (en) | 2017-07-13 | 2018-03-20 | HumanCode, Inc. | Predictive assignments that relate to genetic information and leverage machine learning models |
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| KR102425257B1 (ko) | 2022-07-27 |
| EP4009330A1 (en) | 2022-06-08 |
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| AU2017274412B2 (en) | 2022-07-21 |
| KR20190015371A (ko) | 2019-02-13 |
| EP3465503A1 (en) | 2019-04-10 |
| US20170349944A1 (en) | 2017-12-07 |
| TWI755400B (zh) | 2022-02-21 |
| CA3026061A1 (en) | 2017-12-07 |
| US20200291467A1 (en) | 2020-09-17 |
| MX2018014856A (es) | 2019-03-07 |
| BR112018074572A2 (pt) | 2019-03-12 |
| JP2019529865A (ja) | 2019-10-17 |
| EP3465503B1 (en) | 2021-09-29 |
| TW201800739A (zh) | 2018-01-01 |
| CN109313925B (zh) | 2022-09-02 |
| US10711299B2 (en) | 2020-07-14 |
| WO2017210413A1 (en) | 2017-12-07 |
| AU2017274412A1 (en) | 2018-12-06 |
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