JP6913089B2 - Ｄｎａライブラリーの高効率構築 - Google Patents

Ｄｎａライブラリーの高効率構築 Download PDF

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JP6913089B2
JP6913089B2 JP2018524250A JP2018524250A JP6913089B2 JP 6913089 B2 JP6913089 B2 JP 6913089B2 JP 2018524250 A JP2018524250 A JP 2018524250A JP 2018524250 A JP2018524250 A JP 2018524250A JP 6913089 B2 JP6913089 B2 JP 6913089B2
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dna
oligonucleotide
repair
adapter
ligation
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JP2019504618A5 (OSRAM
JP2019504618A (ja
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クリストファー ケー． レイモンド，
クリストファー ケー． レイモンド，
リー ピー． リム，
リー ピー． リム，
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レゾリューション バイオサイエンス， インコーポレイテッド
レゾリューション バイオサイエンス， インコーポレイテッド
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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JP2018524250A 2015-11-11 2016-11-10 Ｄｎａライブラリーの高効率構築 Active JP6913089B2 (ja)

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US201562254110P 2015-11-11 2015-11-11
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Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10202009015SA (en) 2012-12-10 2020-10-29 Resolution Bioscience Inc Methods for targeted genomic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
CA3004435A1 (en) 2015-11-11 2017-05-18 Resolution Bioscience, Inc. High efficiency construction of dna libraries
US11319594B2 (en) * 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
JP2020527340A (ja) * 2017-06-30 2020-09-10 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア セルフリーｄｎａ中のｄｎａメチル化を評価するための方法およびシステム
CA3075280A1 (en) 2017-09-11 2019-03-14 Ludwig Institute For Cancer Research Ltd Selective labeling of 5-methylcytosine in circulating cell-free dna
AU2018355575A1 (en) 2017-10-27 2020-05-21 Juno Diagnostics, Inc. Devices, systems and methods for ultra-low volume liquid biopsy
EP3762513A1 (en) * 2018-03-08 2021-01-13 St. Johns University Circulating serum cell-free dna biomarkers and methods
CA3095030A1 (en) 2018-03-30 2019-10-03 Juno Diagnostics, Inc. Deep learning-based methods, devices, and systems for prenatal testing
EP3775198A4 (en) 2018-04-02 2022-01-05 Grail, Inc. METHYLATION MARKERS AND TARGETED METHYLATION PROBE PANELS
EP3827011B1 (en) * 2018-07-24 2024-06-19 Salish Bioscience Inc. Methods and composition for targeted genomic analysis
CN113286881A (zh) 2018-09-27 2021-08-20 格里尔公司 甲基化标记和标靶甲基化探针板
US11781169B2 (en) 2018-10-05 2023-10-10 Microsoft Technology Licensing, Llc Enzymatic DNA repair
CN111074353B (zh) * 2018-10-18 2023-10-13 深圳华大智造科技股份有限公司 全基因组甲基化文库单链建库方法和得到的全基因组甲基化文库
US11926821B2 (en) * 2018-10-22 2024-03-12 The Chinese University Of Hong Kong Cell-free DNA quality
CN109576799B (zh) * 2018-11-30 2022-04-26 深圳安吉康尔医学检验实验室 Fh测序文库的构建方法和引物组及试剂盒
EP3947672A4 (en) * 2019-03-27 2023-01-11 Juno Diagnostics, Inc. METHODS, SYSTEMS AND DEVICES FOR OPTIMIZED ULTRA-LOW VOLUME LIQUID BIOPSY
GB202013141D0 (en) * 2020-08-21 2020-10-07 Univ College Cardiff Consultants Ltd A screening method for the detection of DNA damage
CN116490613A (zh) * 2020-09-08 2023-07-25 分析生物科学有限公司 用于高效构建遗传文库和遗传分析的衔接子和方法
CN114317528A (zh) * 2020-09-30 2022-04-12 北京吉因加科技有限公司 特异分子标签umi组、混合特异分子标签接头及应用
CN114438184B (zh) * 2022-04-08 2022-07-12 昌平国家实验室 游离dna甲基化测序文库构建方法及应用

Family Cites Families (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591582A (en) 1985-07-23 1997-01-07 The Board Of Rijks Universiteit Leiden Methods for detecting activated RAS oncogenes
US5888731A (en) 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
CA2268368A1 (en) 1997-08-28 1999-03-11 The Perkin-Elmer Corporation Improved detection of mutations in nucleic acids by chemical cleavage
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6812341B1 (en) 2001-05-11 2004-11-02 Ambion, Inc. High efficiency mRNA isolation methods and compositions
WO2003052116A2 (en) 2001-07-17 2003-06-26 Stratagene Methods for detection of a target nucleic acid using multi-subunit probes
WO2003020979A1 (en) 2001-08-31 2003-03-13 Rosetta Inpharmactis Llc. Methods for preparing nucleic acid samples
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
WO2004053127A1 (ja) * 2002-12-11 2004-06-24 Keio University 対応付け分子およびその構成要素のライブラリーの製造方法および利用方法
TW200506052A (en) 2003-05-07 2005-02-16 Takara Bio Inc Method of analyzing DNA region
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2007006091A1 (en) 2005-07-07 2007-01-18 Athlomics Pty Ltd Polynucleotide marker genes and their expression, for diagnosis of endotoxemia
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1989318B1 (en) 2006-01-06 2014-07-30 Agilent Technologies, Inc. Reaction buffer composition for nucleic acid replication with packed dna polymerases
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008045158A1 (en) 2006-10-10 2008-04-17 Illumina, Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
US12180549B2 (en) 2007-07-23 2024-12-31 The Chinese University Of Hong Kong Diagnosing fetal chromosomal aneuploidy using genomic sequencing
US8067164B2 (en) 2007-08-12 2011-11-29 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
EP2227563B1 (en) * 2007-12-05 2012-06-06 Complete Genomics, Inc. Efficient base determination in sequencing reactions
WO2009091798A1 (en) 2008-01-16 2009-07-23 Helicos Biosciences Corporation Quantitative genetic analysis
US8202691B2 (en) 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
WO2010030683A1 (en) * 2008-09-09 2010-03-18 Rosetta Inpharmatics Llc Methods of generating gene specific libraries
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
WO2010068856A1 (en) 2008-12-11 2010-06-17 Trustees Of Boston University Isolation of rna
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
WO2010129937A2 (en) 2009-05-08 2010-11-11 Life Technologies Corporation Methods for detecting genetic variations in dna samples
DK2669387T3 (en) 2009-08-25 2016-09-19 Illumina Inc Methods of selection and amplification of polynucleotides
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
US10378064B1 (en) 2010-04-16 2019-08-13 Chronix Biomedical Analyzing circulating nucleic acids to identify a biomarker representative of cancer presented by a patient population
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
WO2011156529A2 (en) 2010-06-08 2011-12-15 Nugen Technologies, Inc. Methods and composition for multiplex sequencing
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
IN2013MN00522A (OSRAM) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
EP4269623A3 (en) 2010-11-30 2024-03-06 The Chinese University Of Hong Kong Analysis of a biological sample of an organism for chromosomal deletions or amplifications associated with cancer
JP6054303B2 (ja) 2010-12-30 2016-12-27 ファウンデーション メディシン インコーポレイテッドＦｏｕｎｄａｔｉｏｎ Ｍｅｄｉｃｉｎｅ， Ｉｎｃ． 腫瘍試料の多重遺伝子分析の最適化
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
DK2697392T3 (en) 2011-04-12 2016-03-29 Verinata Health Inc SOLUTION OF GENOME FRACTIONS USING Polymorphism COUNTS
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
CN103103624B (zh) * 2011-11-15 2014-12-31 深圳华大基因科技服务有限公司 高通量测序文库的构建方法及其应用
US10227587B2 (en) 2012-01-10 2019-03-12 Berry Genomics Co., Ltd. Method for constructing a plasma DNA sequencing library
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US20130288915A1 (en) 2012-04-27 2013-10-31 Htg Molecular Diagnostics, Inc. Compositions and methods for alk molecular testing
CA2872141C (en) 2012-05-31 2016-01-19 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
CA2877740A1 (en) 2012-07-03 2014-01-09 Integrated Dna Technologies, Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
US9371568B2 (en) 2012-07-31 2016-06-21 Novartis Ag Markers associated with human double minute 2 inhibitors
EP2880448B1 (en) 2012-08-03 2022-06-29 Foundation Medicine, Inc. Human papilloma virus as predictor of cancer prognosis
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
ES2769241T5 (es) 2012-09-04 2023-05-30 Guardant Health Inc Sistemas y métodos para detectar variación en el número de copias
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CA2886605A1 (en) 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2887094C (en) 2012-10-04 2021-09-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20150291953A1 (en) 2012-11-02 2015-10-15 Enzymatics Inc. Methods and kits for nucleic acid sample preparation for sequencing
HK1214830A1 (zh) 2012-11-05 2016-08-05 Foundation Medicine, Inc. 新型ntrk1融合分子及其应用
SG10202009015SA (en) 2012-12-10 2020-10-29 Resolution Bioscience Inc Methods for targeted genomic analysis
ES2703764T3 (es) 2012-12-14 2019-03-12 Chronix Biomedical Biomarcadores personalizados para el cáncer
US11158425B2 (en) 2013-01-05 2021-10-26 Foundation Medicine, Inc. System and method for managing genomic information
WO2014116881A1 (en) 2013-01-23 2014-07-31 Reproductive Genetics And Technology Solutions, Llc Compositions and methods for genetic analysis of embryos
WO2014122288A1 (en) 2013-02-08 2014-08-14 Qiagen Gmbh Method for separating dna by size
CA2906818C (en) 2013-03-15 2022-08-16 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
CN105518151B (zh) 2013-03-15 2021-05-25 莱兰斯坦福初级大学评议会 循环核酸肿瘤标志物的鉴别和用途
HK1222466A1 (zh) 2013-05-10 2017-06-30 Foundation Medicine, Inc 遗传变异分析
US20150072344A1 (en) 2013-09-10 2015-03-12 Imdaptive Incorporated Barcoded Universal Marker Indicator (BUMI) Tags
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
CN103668471B (zh) * 2013-12-19 2015-09-30 上海交通大学 一种构建dna高通量测序文库的方法及其配套试剂盒
CN111534580B (zh) 2013-12-28 2024-06-04 夸登特健康公司 用于检测遗传变异的方法和系统
DK3604544T3 (en) * 2014-01-31 2022-02-14 Swift Biosciences Inc Improved methods for processing dna substrates
EP3114231B1 (en) 2014-03-03 2019-01-02 Swift Biosciences, Inc. Enhanced adaptor ligation
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
JP6709778B2 (ja) * 2014-08-22 2020-06-17 レゾリューション バイオサイエンス， インコーポレイテッド 無細胞ＤＮＡ（ｃｆＤＮＡ）の定量的遺伝子解析のための方法
JP2017525369A (ja) * 2014-09-05 2017-09-07 キアゲン ゲーエムベーハー アダプター連結アンプリコンの調製
CN107075730A (zh) * 2014-09-12 2017-08-18 利兰·斯坦福青年大学托管委员会 循环核酸的鉴定及用途
WO2016037358A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
EP4112738B1 (en) 2014-12-05 2024-07-24 Foundation Medicine, Inc. Multigene analysis of tumor samples
EP3567120B1 (en) 2014-12-12 2020-08-19 Verinata Health, Inc. Using cell-free dna fragment size to determine copy number variations
ES3017068T3 (en) 2014-12-31 2025-05-12 Guardant Health Inc Detection and treatment of disease exhibiting disease cell heterogeneity and systems and methods for communicating test results
SG11201706529TA (en) 2015-02-10 2017-09-28 Univ Hong Kong Chinese Detecting mutations for cancer screening and fetal analysis
DK3325664T3 (da) 2015-07-23 2022-03-07 Univ Hong Kong Chinese Analyse af fragmenteringsmønstre for cellefrit DNA
EP4462438A3 (en) 2015-10-09 2025-02-26 Guardant Health, Inc. Population based treatment recommender using cell free dna
WO2017062970A1 (en) 2015-10-10 2017-04-13 Guardant Health, Inc. Methods and applications of gene fusion detection in cell-free dna analysis
CA3004435A1 (en) 2015-11-11 2017-05-18 Resolution Bioscience, Inc. High efficiency construction of dna libraries
US10095831B2 (en) 2016-02-03 2018-10-09 Verinata Health, Inc. Using cell-free DNA fragment size to determine copy number variations
WO2017151524A1 (en) 2016-02-29 2017-09-08 Foundation Medicine, Inc. Methods and systems for evaluating tumor mutational burden
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
KR102344635B1 (ko) 2016-09-30 2021-12-31 가던트 헬쓰, 인크. 무세포 핵산의 다중-해상도 분석 방법
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
EP3541934B1 (en) * 2016-11-17 2024-04-10 LGC Clinical Diagnostics, Inc. Methods for preparing dna reference material and controls
CA3268717A1 (en) 2016-12-09 2025-11-29 Ncan Genomics, Inc. Bound ligature
AU2018252018A1 (en) 2017-04-14 2019-10-10 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
WO2020106906A1 (en) 2018-11-21 2020-05-28 Avida Biomed, Inc. Methods for targeted nucleic acid library formation

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