MX2023008338A - Construccion de alta eficacia de bibliotecas de adn. - Google Patents

Construccion de alta eficacia de bibliotecas de adn.

Info

Publication number
MX2023008338A
MX2023008338A MX2023008338A MX2023008338A MX2023008338A MX 2023008338 A MX2023008338 A MX 2023008338A MX 2023008338 A MX2023008338 A MX 2023008338A MX 2023008338 A MX2023008338 A MX 2023008338A MX 2023008338 A MX2023008338 A MX 2023008338A
Authority
MX
Mexico
Prior art keywords
high efficiency
dna libraries
efficiency construction
libraries
construction
Prior art date
Application number
MX2023008338A
Other languages
English (en)
Inventor
Christopher K Raymond
Lee P Lim
Original Assignee
Resolution Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Resolution Bioscience Inc filed Critical Resolution Bioscience Inc
Publication of MX2023008338A publication Critical patent/MX2023008338A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La invención proporciona un método para construir una biblioteca de ADN, el método caracterizado porque comprende: retirar los residuos de fosfato terminal de uno o más fragmentos de ADN; tratar los fragmentos de ADN desfosforilado con una o más enzimas de reparación de extremo para generar ADN de extremo reparado; usar una ligasa para ligar uno o más preadaptadores de ADN de doble cadena (ADNdc) al extremo 3´ de cada cadena de los fragmentos de ADN de extremo reparado para formar complejos de ADN de preadaptador/extremo reparado; desplazar el oligonucleótido de cadena auxiliar que no es de ligación de los complejos de ADN de preadaptador/ extremo reparado con un oligonucleótido de reparación, para formar complejos de ADN de adaptador/ extremo reparado, en donde cada adaptador comprende el oligonucleótido de cadena de ligación y el oligonucleótido de reparación; y tratar los complejos de ADN de adaptador/ extremo reparado con una cinasa de polinucleótido y una ligasa de ADN para formar una biblioteca de fragmentos de ADNdc contiguos, en donde el oligonucleótido de reparación se liga al extremo 5´ de cada cadena de los fragmentos de ADN de extremo reparado.
MX2023008338A 2015-11-11 2018-05-09 Construccion de alta eficacia de bibliotecas de adn. MX2023008338A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562254110P 2015-11-11 2015-11-11

Publications (1)

Publication Number Publication Date
MX2023008338A true MX2023008338A (es) 2023-07-25

Family

ID=58695623

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2018005858A MX2018005858A (es) 2015-11-11 2016-11-10 Construccion de alta eficacia de bibliotecas de adn.
MX2023008338A MX2023008338A (es) 2015-11-11 2018-05-09 Construccion de alta eficacia de bibliotecas de adn.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2018005858A MX2018005858A (es) 2015-11-11 2016-11-10 Construccion de alta eficacia de bibliotecas de adn.

Country Status (14)

Country Link
US (2) US11339391B2 (es)
EP (2) EP3374525B1 (es)
JP (4) JP6913089B2 (es)
KR (1) KR20180113973A (es)
CN (2) CN108431233B (es)
AU (1) AU2016353133B2 (es)
BR (1) BR112018009606A8 (es)
CA (1) CA3004435A1 (es)
DK (1) DK3374525T3 (es)
ES (1) ES2856598T3 (es)
IL (2) IL259237B (es)
MX (2) MX2018005858A (es)
RU (1) RU2018121254A (es)
WO (1) WO2017083562A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201504334WA (en) 2012-12-10 2015-07-30 Resolution Bioscience Inc Methods for targeted genomic analysis
CN108431233B (zh) 2015-11-11 2022-04-01 分析生物科学有限公司 Dna文库的高效率构建
BR112019003704A2 (pt) 2016-08-25 2019-05-28 Resolution Bioscience Inc métodos para a detecção de alterações na cópia genômica em amostras de dna
AU2018291006A1 (en) * 2017-06-30 2020-02-06 The Regents Of The University Of California Methods and systems for evaluating DNA methylation in cell-free DNA
AU2018328324A1 (en) * 2017-09-11 2020-04-30 Ludwig Institute For Cancer Research Ltd Selective labeling of 5-methylcytosine in circulating cell-free DNA
CN111526793A (zh) 2017-10-27 2020-08-11 朱诺诊断学公司 用于超低体积液体活检的设备、系统和方法
US11767562B2 (en) 2018-03-08 2023-09-26 St. John's University Circulating serum cell-free DNA biomarkers and methods
CA3107052A1 (en) * 2018-07-24 2020-01-30 Ripple Biosolutions Llc Methods and composition for targeted genomic analysis
WO2020069350A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
US11781169B2 (en) * 2018-10-05 2023-10-10 Microsoft Technology Licensing, Llc Enzymatic DNA repair
CN111074353B (zh) * 2018-10-18 2023-10-13 深圳华大智造科技股份有限公司 全基因组甲基化文库单链建库方法和得到的全基因组甲基化文库
US11926821B2 (en) * 2018-10-22 2024-03-12 The Chinese University Of Hong Kong Cell-free DNA quality
CN109576799B (zh) * 2018-11-30 2022-04-26 深圳安吉康尔医学检验实验室 Fh测序文库的构建方法和引物组及试剂盒
CA3134941A1 (en) * 2019-03-27 2020-10-01 Juno Diagnostics, Inc. Optimized ultra-low volume liquid biopsy methods, systems, and devices
GB202013141D0 (en) * 2020-08-21 2020-10-07 Univ College Cardiff Consultants Ltd A screening method for the detection of DNA damage
CA3189103A1 (en) * 2020-09-08 2022-03-17 Jiannan GUO Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis
CN114317528A (zh) * 2020-09-30 2022-04-12 北京吉因加科技有限公司 特异分子标签umi组、混合特异分子标签接头及应用

Family Cites Families (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591582A (en) 1985-07-23 1997-01-07 The Board Of Rijks Universiteit Leiden Methods for detecting activated RAS oncogenes
US5888731A (en) 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US5972618A (en) 1997-08-28 1999-10-26 Bloch; Will Detection of mutations in nucleic acids by chemical cleavage
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6812341B1 (en) 2001-05-11 2004-11-02 Ambion, Inc. High efficiency mRNA isolation methods and compositions
US7183052B2 (en) 2001-07-17 2007-02-27 Stratagene California Methods for detection of a target nucleic acid using multi-subunit probes
WO2003020979A1 (en) 2001-08-31 2003-03-13 Rosetta Inpharmactis Llc. Methods for preparing nucleic acid samples
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
WO2004053127A1 (ja) * 2002-12-11 2004-06-24 Keio University 対応付け分子およびその構成要素のライブラリーの製造方法および利用方法
TW200506052A (en) 2003-05-07 2005-02-16 Takara Bio Inc Method of analyzing DNA region
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
CN101501215A (zh) 2005-07-07 2009-08-05 阿什洛米克斯控股有限公司 用于诊断内毒素血症的多核苷酸标记基因及它们的表达
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1989318B1 (en) 2006-01-06 2014-07-30 Agilent Technologies, Inc. Reaction buffer composition for nucleic acid replication with packed dna polymerases
US7537897B2 (en) 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2076609A1 (en) 2006-10-10 2009-07-08 Illumina Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
US20100112590A1 (en) 2007-07-23 2010-05-06 The Chinese University Of Hong Kong Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing With Enrichment
WO2009023676A1 (en) 2007-08-12 2009-02-19 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
WO2009076238A2 (en) * 2007-12-05 2009-06-18 Complete Genomics, Inc. Efficient base determination in sequencing reactions
WO2009091798A1 (en) 2008-01-16 2009-07-23 Helicos Biosciences Corporation Quantitative genetic analysis
US8202691B2 (en) 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
US8999642B2 (en) 2008-03-10 2015-04-07 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
WO2010068856A1 (en) 2008-12-11 2010-06-17 Trustees Of Boston University Isolation of rna
CN103952482A (zh) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US20110003301A1 (en) 2009-05-08 2011-01-06 Life Technologies Corporation Methods for detecting genetic variations in dna samples
US10017812B2 (en) 2010-05-18 2018-07-10 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
US10378064B1 (en) 2010-04-16 2019-08-13 Chronix Biomedical Analyzing circulating nucleic acids to identify a biomarker representative of cancer presented by a patient population
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
CN103119439A (zh) 2010-06-08 2013-05-22 纽亘技术公司 用于多重测序的方法和组合物
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
EP3572528A1 (en) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
KR102185244B1 (ko) 2010-11-30 2020-12-02 더 차이니즈 유니버시티 오브 홍콩 암과 연관된 유전적 또는 분자적 이상들의 검출
KR20210131432A (ko) 2010-12-30 2021-11-02 파운데이션 메디신 인코포레이티드 종양 샘플의 다유전자 분석의 최적화
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
CA2832468C (en) 2011-04-12 2023-10-31 Verinata Health, Inc. Resolving genome fractions using polymorphism counts
WO2012142213A2 (en) 2011-04-15 2012-10-18 The Johns Hopkins University Safe sequencing system
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
CN103103624B (zh) * 2011-11-15 2014-12-31 深圳华大基因科技服务有限公司 高通量测序文库的构建方法及其应用
US10227587B2 (en) 2012-01-10 2019-03-12 Berry Genomics Co., Ltd. Method for constructing a plasma DNA sequencing library
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
EP4234713A3 (en) 2012-03-20 2024-02-14 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US20130288915A1 (en) 2012-04-27 2013-10-31 Htg Molecular Diagnostics, Inc. Compositions and methods for alk molecular testing
WO2013181170A1 (en) 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
EP2872629B1 (en) 2012-07-03 2019-09-04 Integrated DNA Technologies Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
US9371568B2 (en) 2012-07-31 2016-06-21 Novartis Ag Markers associated with human double minute 2 inhibitors
US8673972B2 (en) 2012-08-03 2014-03-18 Foundation Medicine, Inc. Human papilloma virus as predictor of cancer prognosis
ES2906714T3 (es) 2012-09-04 2022-04-20 Guardant Health Inc Métodos para detectar mutaciones raras y variación en el número de copias
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2900836B1 (en) 2012-09-28 2023-05-10 Cepheid Two-primer pcr for microrna multiplex assay
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2904534B1 (en) 2012-10-04 2021-12-15 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014071295A1 (en) 2012-11-02 2014-05-08 Enzymatics Inc. Methods and kits for nucleic acid sample preparation for sequencing
WO2014071358A2 (en) 2012-11-05 2014-05-08 Foundation Medicine, Inc. Novel ntrk1 fusion molecules and uses thereof
SG11201504334WA (en) 2012-12-10 2015-07-30 Resolution Bioscience Inc Methods for targeted genomic analysis
ES2703764T3 (es) 2012-12-14 2019-03-12 Chronix Biomedical Biomarcadores personalizados para el cáncer
US11158425B2 (en) 2013-01-05 2021-10-26 Foundation Medicine, Inc. System and method for managing genomic information
US20140242581A1 (en) 2013-01-23 2014-08-28 Reproductive Genetics And Technology Solutions, Llc Compositions and methods for genetic analysis of embryos
EP2954054B1 (en) 2013-02-08 2018-12-05 Qiagen GmbH Method for separating dna by size
EP2971097B1 (en) * 2013-03-15 2018-08-01 Verinata Health, Inc Generating cell-free dna libraries directly from blood
EP3795696B1 (en) 2013-03-15 2023-04-26 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US9792403B2 (en) 2013-05-10 2017-10-17 Foundation Medicine, Inc. Analysis of genetic variants
US20150072344A1 (en) 2013-09-10 2015-03-12 Imdaptive Incorporated Barcoded Universal Marker Indicator (BUMI) Tags
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN103668471B (zh) * 2013-12-19 2015-09-30 上海交通大学 一种构建dna高通量测序文库的方法及其配套试剂盒
ES2822125T3 (es) 2013-12-28 2021-04-29 Guardant Health Inc Métodos y sistemas para detectar variantes genéticas
AU2015210705B2 (en) * 2014-01-31 2020-11-05 Integrated Dna Technologies, Inc. Improved methods for processing DNA substrates
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
CN115029342A (zh) * 2014-08-22 2022-09-09 分析生物科学有限公司 用于无细胞dna的定量遗传分析的方法
WO2016034433A1 (en) * 2014-09-05 2016-03-10 Qiagen Gmbh Preparation of adapter-ligated amplicons
EP3191628B1 (en) * 2014-09-12 2022-05-25 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acids
AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
CA2967447A1 (en) 2014-12-05 2016-06-09 Foundation Medicine, Inc. Multigene analysis of tumor samples
AU2015360298B2 (en) 2014-12-12 2018-06-07 Verinata Health, Inc. Using cell-free DNA fragment size to determine copy number variations
CN113930507A (zh) 2014-12-31 2022-01-14 夸登特健康公司 疾病的检测和治疗以及用于传送测试结果的系统和方法
WO2016127944A1 (en) 2015-02-10 2016-08-18 The Chinese University Of Hong Kong Detecting mutations for cancer screening and fetal analysis
JP6931236B2 (ja) 2015-07-23 2021-09-01 ザ チャイニーズ ユニバーシティ オブ ホンコン 無細胞ｄｎａの断片化パターンの分析
CN108474040B (zh) 2015-10-09 2023-05-16 夸登特健康公司 使用无细胞dna的基于群体的治疗推荐
US20180300449A1 (en) 2015-10-10 2018-10-18 Guardant Health, Inc. Methods and applications of gene fusion detection in cell-free dna analysis
CN108431233B (zh) 2015-11-11 2022-04-01 分析生物科学有限公司 Dna文库的高效率构建
US10095831B2 (en) 2016-02-03 2018-10-09 Verinata Health, Inc. Using cell-free DNA fragment size to determine copy number variations
KR102358206B1 (ko) 2016-02-29 2022-02-04 파운데이션 메디신 인코포레이티드 종양 돌연변이 부담을 평가하기 위한 방법 및 시스템
WO2017214557A1 (en) 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
BR112019003704A2 (pt) 2016-08-25 2019-05-28 Resolution Bioscience Inc métodos para a detecção de alterações na cópia genômica em amostras de dna
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
CA3027919C (en) 2016-09-30 2023-02-28 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
AU2017363180B2 (en) * 2016-11-17 2023-11-02 LGC Clinical Diagnostics, Inc. Methods for preparing DNA reference material and controls
CA3048420A1 (en) 2016-12-09 2018-06-14 Boreal Genomics, Inc. Linked ligation
ES2962223T3 (es) 2017-04-14 2024-03-18 Guardant Health Inc Métodos para unir adaptadores a ácidos nucleicos de muestra
KR20210141915A (ko) 2018-11-21 2021-11-23 아비다 바이오메드 인코포레이티드 표적화된 핵산 라이브러리 형성을 위한 방법

Also Published As

Publication number Publication date
BR112018009606A8 (pt) 2019-02-26
ES2856598T3 (es) 2021-09-27
RU2018121254A (ru) 2019-12-16
IL259237B (en) 2021-08-31
JP6913089B2 (ja) 2021-08-04
JP2023113960A (ja) 2023-08-16
DK3374525T3 (da) 2021-04-06
JP2021073252A (ja) 2021-05-13
BR112018009606A2 (pt) 2018-11-06
IL259237A (en) 2018-07-31
EP3374525A4 (en) 2019-05-29
IL285202A (en) 2021-08-31
EP3889257A1 (en) 2021-10-06
EP3374525A1 (en) 2018-09-19
JP2022105062A (ja) 2022-07-12
EP3374525B1 (en) 2021-01-20
JP7318054B2 (ja) 2023-07-31
CN115044645A (zh) 2022-09-13
JP7223788B2 (ja) 2023-02-16
AU2016353133B2 (en) 2022-12-08
WO2017083562A1 (en) 2017-05-18
US11339391B2 (en) 2022-05-24
CN108431233B (zh) 2022-04-01
CN108431233A (zh) 2018-08-21
KR20180113973A (ko) 2018-10-17
MX2018005858A (es) 2019-02-20
AU2016353133A1 (en) 2018-05-24
US20180245072A1 (en) 2018-08-30
JP2019504618A (ja) 2019-02-21
US20220267763A1 (en) 2022-08-25
CA3004435A1 (en) 2017-05-18

Similar Documents

Publication Publication Date Title
MX2023008338A (es) Construccion de alta eficacia de bibliotecas de adn.
HK1250182A1 (zh) 核酸分析系統和方法
MX2016016713A (es) Procesos y sistemas para el montaje de secuencias de acido nucleico.
EP3766988C0 (en) HIGH THROUGHPUT ANALYSIS OF SINGLE CELLS COMBINING PROTEOMIC AND GENOMIC INFORMATION
IL253829A0 (en) System and method for genetic analysis of isolated cells
IL251261B (en) Reagents and methods for the identification, enrichment and/or expansion of antigen-specific T-cells
BR112016007401A2 (pt) métodos e processos para avaliação não invasiva das variações genéticas
EP3194627C0 (en) METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACID SEQUENCING DATA
TW201614076A (en) Methods for pathogen detection and disease management on meats, plants, or plant parts
BR112012009284A2 (pt) método e sistema para avaliar automaticamente a semelhança entre uma região geológica alvo de interesse e uma pluralidade de regiões geológicas conhecidas de interesse.
EP3283643B8 (en) Means for diagnosing, predicting or monitoring pneumocystis pneumonia
EP3356832A4 (en) BIOMARKERS AND METHODS FOR ASSESSING THE ACTIVITY OF PSORIASTIC ARTHRITIS DISEASE
EP3335140A4 (en) SYSTEMS, APPARATUS AND METHODS FOR ANALYZING AND PREDICTING CELLULAR TRACKS
GB201616668D0 (en) Sequence data analyzer, DNA analysis system and sequence data analysis method
MX2016008447A (es) Metodos y sistemas para amplificacion de acido nucleico.
EA201400940A1 (ru) Способ получения медетомидина
EP3693742A3 (en) Methods of detecting prostate cancer
IL263960A (en) DNA library production method and genomic DNA analysis method using the DNA library
GB201511372D0 (en) Methods, systems and tools for selecting subjects suffering from neurodegerative disease
AU2017287059A1 (en) Method for producing DNA library and method for analyzing genomic DNA using DNA library
MX2016013877A (es) Composiciones y metodos para mejorar y/o predecir la amplificacion de adn.
EP3232360A4 (en) Malware analysis system, malware analysis method, and malware analysis program
GB201906155D0 (en) Method and system for collecting, visualising and analysing risk data
FR3041492B1 (fr) Procede de connexion securise, depuis un equipement informatique client, a une ressource informatique.
WO2019051266A3 (en) Method of using biomarkers and clinical variables for predicting chemotherapy benefit