JP6898251B2 - 組換え抗体分子の単量体化方法 - Google Patents

組換え抗体分子の単量体化方法 Download PDF

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JP6898251B2
JP6898251B2 JP2017555223A JP2017555223A JP6898251B2 JP 6898251 B2 JP6898251 B2 JP 6898251B2 JP 2017555223 A JP2017555223 A JP 2017555223A JP 2017555223 A JP2017555223 A JP 2017555223A JP 6898251 B2 JP6898251 B2 JP 6898251B2
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antibody
concentration
fab
reducing agent
urea
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JP2018515449A5 (https=
JP2018515449A (ja
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フィリップ ヘイウッド、サム
フィリップ ヘイウッド、サム
バリー ワイルド、ギャヴィン
バリー ワイルド、ギャヴィン
ハニフ、ラズワン
パージ、クリストファー ジョン ル
パージ、クリストファー ジョン ル
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ユーシービー バイオファルマ エスアールエル
ユーシービー バイオファルマ エスアールエル
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39591—Stabilisation, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/10—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton
    • C07C323/11—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/12—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/23—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton
    • C07C323/24—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/25—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/12—Cyclic peptides with only normal peptide bonds in the ring
    • C07K5/123—Tripeptides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2017555223A 2015-04-22 2016-04-22 組換え抗体分子の単量体化方法 Expired - Fee Related JP6898251B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1506870.3 2015-04-22
GBGB1506870.3A GB201506870D0 (en) 2015-04-22 2015-04-22 Method
PCT/EP2016/059051 WO2016170138A1 (en) 2015-04-22 2016-04-22 Method of monomerisation of recombinant antibody molecules

Publications (3)

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JP2018515449A JP2018515449A (ja) 2018-06-14
JP2018515449A5 JP2018515449A5 (https=) 2019-05-23
JP6898251B2 true JP6898251B2 (ja) 2021-07-07

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US (2) US10828366B2 (https=)
EP (1) EP3286204B1 (https=)
JP (1) JP6898251B2 (https=)
KR (1) KR20170138552A (https=)
CN (1) CN108064230B (https=)
AU (1) AU2016251620A1 (https=)
BR (1) BR112017021812A2 (https=)
CA (1) CA2981644C (https=)
CL (1) CL2017002676A1 (https=)
CO (1) CO2017010385A2 (https=)
EA (1) EA201792329A1 (https=)
ES (1) ES2846732T3 (https=)
GB (1) GB201506870D0 (https=)
IL (1) IL254765A0 (https=)
MX (1) MX2017013376A (https=)
SG (1) SG11201708151TA (https=)
WO (1) WO2016170138A1 (https=)

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AU2019356564A1 (en) * 2018-10-11 2021-04-29 Amgen Inc. Downstream processing of bispecific antibody constructs
JP7607564B2 (ja) * 2018-12-21 2024-12-27 ノバルティス アーゲー Ｐｍｅｌ１７に対する抗体及びその結合体
WO2022122654A1 (en) * 2020-12-07 2022-06-16 UCB Biopharma SRL Multi-specific antibodies and antibody combinations

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GB201506870D0 (en) 2015-06-03
IL254765A0 (en) 2017-12-31
AU2016251620A1 (en) 2017-10-26
US20210069328A1 (en) 2021-03-11
CN108064230B (zh) 2021-04-06
EP3286204A1 (en) 2018-02-28
SG11201708151TA (en) 2017-11-29
CN108064230A (zh) 2018-05-22
BR112017021812A2 (pt) 2018-07-10
CA2981644A1 (en) 2016-10-27
EA201792329A1 (ru) 2018-02-28
JP2018515449A (ja) 2018-06-14
EP3286204B1 (en) 2020-11-18
US11786593B2 (en) 2023-10-17
KR20170138552A (ko) 2017-12-15
WO2016170138A1 (en) 2016-10-27
CO2017010385A2 (es) 2018-03-20
CL2017002676A1 (es) 2018-03-23
MX2017013376A (es) 2017-12-07
ES2846732T3 (es) 2021-07-29
US20180117153A1 (en) 2018-05-03
CA2981644C (en) 2023-07-04
US10828366B2 (en) 2020-11-10

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