JP6872182B2 - 核酸分析および定量化のための方法ならびにシステム - Google Patents

核酸分析および定量化のための方法ならびにシステム Download PDF

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JP6872182B2
JP6872182B2 JP2019521723A JP2019521723A JP6872182B2 JP 6872182 B2 JP6872182 B2 JP 6872182B2 JP 2019521723 A JP2019521723 A JP 2019521723A JP 2019521723 A JP2019521723 A JP 2019521723A JP 6872182 B2 JP6872182 B2 JP 6872182B2
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acid molecules
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JP2019535247A (ja
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ザヤック，アンドリュー
フン，ジュ−スン
グレッグ キング，ハワード
グレッグ キング，ハワード
コックス，デイビッド
デュエック，メーガン
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コンビナティ インコーポレイテッド
コンビナティ インコーポレイテッド
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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JP2019521723A 2016-11-17 2017-11-16 核酸分析および定量化のための方法ならびにシステム Active JP6872182B2 (ja)

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JP2021061372A JP7490610B2 (ja) 2016-11-17 2021-03-31 核酸分析および定量化のための方法ならびにシステム
JP2023206434A JP7709505B2 (ja) 2016-11-17 2023-12-06 核酸分析および定量化のための方法ならびにシステム

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US201662423601P 2016-11-17 2016-11-17
US62/423,601 2016-11-17
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EP (4) EP4617381A3 (https=)
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KR (3) KR102300480B1 (https=)
CN (2) CN110177621B (https=)
AU (3) AU2017363076B2 (https=)
CA (1) CA3042724A1 (https=)
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Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
AU2017363076B2 (en) 2016-11-17 2021-04-29 Combinati Incorporated Methods and systems for nucleic acid analysis and quantification
JP2021511056A (ja) 2018-01-22 2021-05-06 ルミネックス コーポレーション 不連続融解分析のための方法および組成物
CN110835645B (zh) * 2018-08-16 2022-10-21 思纳福(北京)医疗科技有限公司 数字pcr检测方法
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3610947B1 (en) 2018-08-17 2021-04-21 F. Hoffmann-La Roche AG Microfluidic system for digital polymerase chain reaction of a biological sample, and respective method
AU2019397371B2 (en) 2018-12-10 2025-09-18 Combinati Incorporated Microfluidic array for sample digitization
CN109920474B (zh) * 2019-03-14 2021-03-02 深圳市检验检疫科学研究院 绝对定量方法、装置、计算机设备和存储介质
US11060138B1 (en) * 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
KR20230024417A (ko) * 2020-06-22 2023-02-20 콤비네티 인코포레이티드 생물학적 샘플을 분석하기 위한 시스템 및 방법
CN116368213A (zh) 2020-09-28 2023-06-30 康比纳蒂股份有限公司 用于样品处理的装置和方法
AU2022316142A1 (en) 2021-07-21 2024-02-22 Element Biosciences, Inc. Optical systems for nucleic acid sequencing and methods thereof
US20230028814A1 (en) * 2021-07-26 2023-01-26 Mound Laser & Photonics Center, Inc. Method for delivering a chemistry to microchambers via a channel
WO2023023533A1 (en) 2021-08-19 2023-02-23 Luminex Corporation Digital amplification assay analysis method
WO2023043848A1 (en) 2021-09-14 2023-03-23 Combinati, Inc. Microfluidic device and method for processing biological samples
KR20230067718A (ko) * 2021-11-08 2023-05-17 삼성전자주식회사 유전자 증폭 장치 및 방법
US20230348957A1 (en) 2021-12-07 2023-11-02 Luminex Corporation Methods and compositions for nucleic acid analysis

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
NZ502303A (en) 1997-06-18 2002-02-01 Ulrich J Krull Nucleic acid biosensor system & diagnostic use
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
US6361958B1 (en) 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
US6569674B1 (en) 1999-12-15 2003-05-27 Amersham Biosciences Ab Method and apparatus for performing biological reactions on a substrate surface
AU2082701A (en) 1999-12-09 2001-06-18 Motorola, Inc. Multilayered microfluidic devices for analyte reactions
US20050009101A1 (en) 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels
WO2003050035A2 (en) 2001-12-06 2003-06-19 Nanostream, Inc. Adhesiveless microfluidic device fabrication
WO2004040001A2 (en) 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
US7169602B2 (en) 2002-12-04 2007-01-30 Applera Corporation Sample substrate for use in biological testing and method for filling a sample substrate
US20060094108A1 (en) * 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
US20070099290A1 (en) 2003-09-02 2007-05-03 Kazuhiro Iida Customizable chip and method of manufacturing the same
US20050226779A1 (en) * 2003-09-19 2005-10-13 Oldham Mark F Vacuum assist for a microplate
JP2005329333A (ja) 2004-05-20 2005-12-02 Japan Science & Technology Agency マイクロ脱気デバイス
US7608160B2 (en) 2004-10-13 2009-10-27 Rheonix, Inc. Laminated microfluidic structures and method for making
US8715446B2 (en) 2004-10-13 2014-05-06 Rheonix, Inc. Latent solvent-based microfluidic apparatus, methods, and applications
DE102004063438A1 (de) 2004-12-23 2006-07-06 Oktavia Backes Neuartige mikrofluidische Probenträger
EP1885839B1 (en) 2005-04-26 2018-08-08 Life Technologies Corporation Systems and methods for multiple analyte detection
JP2008541055A (ja) * 2005-05-03 2008-11-20 アプレラ コーポレイション 蛍光検出システムおよびそれとともに使用するための色素セット
CN101351542A (zh) 2005-07-15 2009-01-21 阿普尔拉公司 液体加工装置和方法
BRPI0619273A2 (pt) 2005-10-26 2011-09-20 Gen Electric Métodos e sistemas para o fornecimento de amostras fluidas para grupos sensores
GB2445738A (en) 2007-01-16 2008-07-23 Lab901 Ltd Microfluidic device
US9550184B2 (en) 2007-02-05 2017-01-24 Shimadzu Corporation Reactor plate and reaction processing method
EP2816122A1 (en) 2008-02-15 2014-12-24 Mayo Foundation For Medical Education And Research Detecting neoplasm from a stool sample
EP2255010B1 (en) 2008-02-20 2018-05-30 Streck Inc. Thermocycler and sample vessel for rapid amplification of dna
US9487822B2 (en) * 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
JP2009236555A (ja) 2008-03-26 2009-10-15 Shimadzu Corp 流体デバイス及びその製造方法
US20090250347A1 (en) 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic devices & processes for electrokinetic transport
SG174617A1 (en) * 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
EP2248915B1 (en) * 2009-05-05 2013-09-18 Qiagen GmbH Detection of multiple nucleic acid sequences in a reaction cartridge
US9238346B2 (en) 2009-10-08 2016-01-19 National Research Council Of Canada Microfluidic device, composition and method of forming
CN103097883B (zh) * 2010-03-09 2016-03-02 网络百奥有限公司 提供样本输入至结果输出处理的单体生物芯片以及制造方法
US8720036B2 (en) * 2010-03-09 2014-05-13 Netbio, Inc. Unitary biochip providing sample-in to results-out processing and methods of manufacture
US8376317B2 (en) 2010-03-29 2013-02-19 Purdue Research Foundation Microfluidic purge valve
EP3236260B1 (en) 2011-04-13 2020-09-30 Akonni Biosystems, Inc. Method for making a microarray assembly
US9902990B2 (en) * 2011-05-27 2018-02-27 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
WO2013130910A1 (en) 2012-02-29 2013-09-06 Integenx Inc. Sample preparation, processing and analysis systems
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
WO2013101741A1 (en) * 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
WO2013177206A2 (en) * 2012-05-21 2013-11-28 Fluidigm Corporation Single-particle analysis of particle populations
WO2014039963A1 (en) * 2012-09-10 2014-03-13 Biofire Diagnostics, Inc. Multiple amplification cycle detection
CN105164246B (zh) * 2013-03-15 2018-09-25 富鲁达公司 用于分析定义的多细胞组合的方法和设备
DE102013205412A1 (de) 2013-03-27 2014-10-02 Robert Bosch Gmbh Normal-geschlossenes Ventil für mikrofluidische Bauteile aus einem polymeren Schichtsystem sowie Verfahren
WO2015021425A1 (en) * 2013-08-09 2015-02-12 The Regents Of The University Of California Digital fluid sample separation apparatus and methods for one-step quantitative sample analysis
WO2015023616A2 (en) * 2013-08-12 2015-02-19 The Johns Hopkins University Method for comprehensive, quantitative, and highly sensitive discrimination of nucleic acid sequences in homogeneous and heterogeneous populations
JP6427753B2 (ja) * 2013-09-11 2018-11-28 国立大学法人大阪大学 熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法
EP3077806B1 (en) 2013-12-06 2019-09-11 Ortho Clinical Diagnostics, Inc. Assay device having a wash port
KR101562318B1 (ko) 2014-02-10 2015-10-22 나노바이오시스 주식회사 미세유체 칩 및 이를 이용한 실시간 분석 장치
EP3117221B1 (en) * 2014-03-13 2020-09-09 Genapsys Inc. Microfluidic devices and methods for sample preparation and analysis
CN204727866U (zh) 2014-03-31 2015-10-28 博奥生物集团有限公司 一种集核酸扩增和微阵列检测于一体的微流控装置
CN105358673A (zh) * 2014-05-21 2016-02-24 卡尤迪生物科技（北京）有限公司 用于热循环的系统和方法
CA2982884C (en) * 2015-04-24 2021-03-30 F. Hoffmann-La Roche Ag Digital pcr systems and methods using digital microfluidics
CN104877905A (zh) 2015-05-04 2015-09-02 清华大学深圳研究生院 细胞体外共培养微流控芯片、系统及方法
CN104894106A (zh) 2015-05-23 2015-09-09 浙江大学 高集成度等距等分核酸扩增微流控芯片及应用
US9845499B2 (en) * 2016-04-04 2017-12-19 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
US10913063B2 (en) * 2016-07-12 2021-02-09 EMULATE, Inc. Removing bubbles in a microfluidic device
AU2017363076B2 (en) 2016-11-17 2021-04-29 Combinati Incorporated Methods and systems for nucleic acid analysis and quantification
EP3346262A1 (en) 2017-01-10 2018-07-11 Universite d'Aix-Marseille (AMU) Optical thermal method and system for diagnosing pathologies
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
PL425106A1 (pl) 2018-03-30 2019-10-07 Bacteromic Spółka Z Ograniczoną Odpowiedzialnością Chip mikroprzepływowy
EP3774052A4 (en) 2018-04-04 2022-01-05 Combinati Incorporated MICROFLUIDIC SUCTION ARRANGEMENT FOR NUCLEIC ACID QUANTIFICATION
AU2019397371B2 (en) 2018-12-10 2025-09-18 Combinati Incorporated Microfluidic array for sample digitization
JP7467061B2 (ja) * 2019-10-09 2024-04-15 株式会社ジャパンディスプレイ 検出装置
BR112022014571A2 (pt) 2020-01-24 2022-10-04 Combinati Incorporated Métodos e sistemas para fabricação de dispositivo microfluídico
KR20230024417A (ko) 2020-06-22 2023-02-20 콤비네티 인코포레이티드 생물학적 샘플을 분석하기 위한 시스템 및 방법
CN116368213A (zh) 2020-09-28 2023-06-30 康比纳蒂股份有限公司 用于样品处理的装置和方法
WO2022067184A1 (en) 2020-09-28 2022-03-31 Combinati Incorporated Devices and methods for sample processing

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