JP6836512B2 - 組織透過性ペプチドと抗血管内皮細胞成長因子製剤が融合された融合タンパク質を有効成分として含む眼疾患の予防及び治療用薬学的組成物 - Google Patents
組織透過性ペプチドと抗血管内皮細胞成長因子製剤が融合された融合タンパク質を有効成分として含む眼疾患の予防及び治療用薬学的組成物 Download PDFInfo
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Classifications
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- A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
- A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
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- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
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- C07K14/475—Growth factors; Growth regulators
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
Description
組織透過性ペプチドが融合されたラニビズマブ改良体製作
ラニビズマブの低い生産収率の問題は、Fab断片の低い培養生産性とペリプラズム発現を通じた生産タンパク質の多形性で複雑な後処理工程が必要なためである。これを克服するために、点突然変異の導入などのタンパク質工学を介してラニビズマブの特定アミノ酸配列を変換させることにより、生産されたタンパク質が殆ど細胞外に排出されるようにして発現率を高め、均一な形態のタンパク質だけを生産するように生産細胞株を最適化してラニビズマブ変異体であるIDB0061を生産した。これを介して培養生産性を高めて精製工程を単純化して生産収率が画期的に改善されたことを確認した。
本発明者らは抗VEGF製剤であるラニビズマブの効能増大及び耐性克服のために、ラニビズマブのC末端に、ニューロピリン−1(NRP1)及び2に全て結合するか又はニューロピリン−1のみに特異的に結合できる組織透過性ペプチド(tissue penetrating peptide、TPP)の融合を試み、TPPのアミノ酸配列は、下記表3に示した。表3の配列目録中、配列番号1〜4のアミノ酸配列を有するTPPはニューロピリン−1及び2に全て結合が可能であり、配列番号5〜7のアミノ酸配列を有するTPPはニューロピリン−1にのみ特異的に結合が可能である。ラニビズマブにTPPが融合した融合タンパク質の模式図を図1に示した。具体的にはエイプリルバイオ社に依頼して、前記実施例1−1で製作したラニビズマブ変異体IDB0061のC末端に下記表3に記載された様々なTPP(ニューロピリン受容体に特異的に結合するペプチド)が融合された融合タンパク質及びそれを生産する細胞株を製作した。
前記実施例1−2で製造した多様なラニビズマブ改良体の候補タンパク質に対してニューロピリン受容体に対する親和性と内皮細胞間のタイトジャンクション(tight junction)瓦解能を比較した。
前記実施例1−1と同様の方法でIDB0062の生産性を確認した。その結果は前記表1に示した通り、IDB0061にTPP#2を融合したIDB0062も、やはりラニビズマブと比べて5倍ほど高い生産性を示すことを確認した。
ラニビズマブ改良体IDB0062に続いて、実施例1−3でVE-cadherin阻害効果が確認されたTPP#5がリンカーによって連結されて融合したラニビズマブ改良体IDB0064を製作して生産性を確認した。
ラニビズマブ改良体IDB0062の二価(bivalent)の特性確認
ラニビズマブ改良体IDB0062のVEGF-A及びニューロピリン1受容体に対する結合力を確認した。
IDB0062とIDB0072のNRP1に対する結合能を確認するためにSPR分析を行った。EDC/NHS混合液でBiacore CM5 chipを活性化させた後、ターゲットタンパク質であるNRP1を固定化バッファー(10mM sodium acetate、pH5.5)に希釈してRmax:200で計算して最終79Ruに固定した。その後IDB0062、IDB0072試料を12.5nM〜400nMまでHBSEPバッファーで希釈して分析を行った。分析流速は30μl/minで行い、結果のグラフをもとにセンサーグラムを得てKd値を算出した。
ELISAプレート(SPL、Immunoplate Maxi binding)にNRP1(独自生産)を炭酸塩コーティングバッファー(carbonate coating buffer)(0.1M NaHCO3,pH 9.6)に希釈して10μg/mlの最終濃度にしてウェル当り100μlずつ入れ、37℃で2時間コーティングした。以後プレートを3回洗浄して37℃で1時間ブロッキング(4%skim milk、pH 7.4)を行い3回洗浄後、それぞれの試料を適切な倍数で希釈してウェル当り100μlずつ処理し、37℃で1時間反応させた。試料反応終了後、プレートを3回洗浄し、抗ヒトκL鎖抗体-ペルオキシダーゼ標識-ヤギ宿主抗体(Sigma Aldrich、A7164)をブロッキングバッファーに5000倍に希釈してウェル当り100μlずつ処理して、37℃で1時間反応させた。プレートを5回洗浄した後TMB基質(Bethyl、E102)をウェル当り100μlずつ処理して、2〜3分反応後、停止溶液(1N HCl)をウェル当り100μl処理して反応を終了しELISAプレートリーダーを用いて450nmでの値を測定した。
ELISAプレート(SPL、Immunoplate Maxi binding)にVEGF(R&D system、293-VE-500 / CF)を炭酸塩コーティングバッファー(0.1M NaHCO3、pH 9.6)に希釈して、3μg/mlの最終濃度に調製してウェル当り100μlずつ入れて37℃で2時間コーティングした。その後プレートを3回洗浄して37℃で1時間ブロッキング(4%skim milk、pH 7.4)を進めて3回洗浄後、それぞれの試料を適切な倍数で希釈してウェル当り100μlずつ処理して、37℃で1時間反応させる。試料反応終了後、プレートを3回洗浄した後、抗ヒトκL鎖抗体-ペルオキシダーゼ標識-ヤギ宿主抗体(Sigma Aldrich、A7164)をブロッキングバッファーで5000倍に希釈してウェル当り100μlずつ処理して37℃で30分間反応させた。プレートを7回洗浄した後TMB基質(Bethyl、E102)をウェル当り100μlずつ処理して2〜3分反応後、停止溶液(1N HCl)をウェル当り100μl処理して反応を終了し、ELISAプレートリーダーを利用して450nmで値を測定した。
ラニビズマブ改良体IDB0062の安定性評価
抗VEGF製剤改良体の眼球組織透過度評価
IDB0062のC末端に融合したTPPが眼球内皮細胞に多く分布するニューロピリン受容体との結合を介して、実際に組織透過度を改善できるか否かを確認するために、摘出した眼球をFITC標識されたラニビズマブ及びIDB0062溶液に浸した後、時間別の透過度を比較した。
抗体断片(Fab)に比べてベバシズマブ(bevacizumab)のような抗体全長(whole antibody)タンパク質は分子量も大きくタンパク質の3次構造も複雑で組織透過に不利な特性を有する、それにも拘らずベバシズマブのC末端組織透過性ペプチドを融合することにより、このような物理的な限界を克服し、対照薬剤であるベバシズマブに比べて透過度が改善されるか否かを確認するために同じ実験を行った。
角膜血管新生モデルを利用したラニビズマブ改良体の効能評価
IDB0062とラニビズマブの血管新生阻害効果を比較するために、アルカリ熱傷で誘導した角膜血管新生モデルを次のように作製した。セルロースフィルターペーパーを直径2mm円にカットして準備した後、1M NaOH溶液に浸しておいた。マウスは6週齢の雌C57BL/6を使用し、麻酔(Zoletil 40mg/kg+Rompun 5mg/kg、腹腔内投与)後、左眼角膜にNaOHフィルターペーパーを載せて30秒間アルカリ熱傷を誘導した後、40ml PBSで十分に洗浄して角膜血管新生動物モデルを構築した。予防モデルの場合アルカリ熱傷を誘導した当日から薬剤を処理したが、各薬剤は5mg/mlの濃度で1回5μlずつ、1日4回点眼投与を行い、合計5日間投与を行った。投与が終了した後、眼球を摘出して4%パラホルムアルデヒド溶液に1時間浸して固定し、PBSで洗浄した後、解剖顕微鏡を利用して角膜を分離した。分離された角膜は再び4%パラホルムアルデヒド溶液に12時間追加固定した。固定された角膜は、PBSで洗浄した後、常温でブロッキングバッファー(PBS、0.3%BSA、0.1%Triton X100)で2時間反応させる。血管内皮細胞のマーカーであるPECAM-1(CD31)に特異的な一次抗体(BD pharmingen)と周皮細胞マーカーであるNG-2に特異的な一次抗体(Millipore)を冷蔵で終夜反応させて二次蛍光抗体(Alexa Fluor488、Alexa Fluor594、Life Technologies)は、常温で4時間反応して組織を染色した。染色過程が終了した角膜はスライドに移した後、解剖顕微鏡を用いて、角膜の中心部に向けて四方向に切開腺を入れて封入した。封入が完了した角膜のスライドは、蛍光顕微鏡/共焦点顕微鏡を用いて血管と周皮細胞の様相を確認した。
耐性モデルは、予防モデルと同じ方法でアルカリ熱傷を誘導して角膜新生血管が十分に生成できるように10日間放置した。投与濃度と周期は予防モデルと同じく、投与はアルカリ熱傷10日後から10日間行い生成された新生血管を減少させる程度をラニビズマブと比較した(以降、角膜スライドを製作する過程は予防モデルと同一である)。
脈絡膜血管新生モデルを利用したラニビズマブ改良体の効能評価
酸素誘導網膜症モデルを利用したラニビズマブ改良体の効能評価
眼球内注射後の薬剤の分布分析
Claims (10)
- 組織透過性ペプチドと抗血管内皮細胞成長因子(anti-VEGF)が融合した融合タンパク質を有効成分として含む眼疾患の予防及び治療用薬学的組成物であって、
前記組織透過性ペプチドが配列番号1〜7からなる群より選ばれたいずれか一つのアミノ酸配列を含み、
前記抗血管内皮細胞成長因子(anti-VEGF)が、ラニビズマブ(ranibizumab)、ベバシズマブ(bevacizumab)、アフリベルセプト(aflibercept)、コンバーセプト(Conbercept)、r84、CT01、DOM15-10-11、DOM15-26-593、CT-322、ESBA903、及びEPI-0030並びにこれらの変異体で構成された群から選ばれる、眼疾患の予防及び治療用薬学的組成物。 - 前記変異体は、重鎖定常1ドメイン及び軽鎖定常ドメインのアミノ酸であるシステインが欠失しているか、又はシステインを除いてセリンを含む他のアミノ酸残基に置換されたことを特徴とする請求項1に記載の薬学的組成物。
- 前記変異体は、配列番号8で表示される軽鎖及び配列番号10で表示される重鎖からなるラニビズマブ変異体であることを特徴とする請求項1に記載の薬学的組成物。
- 前記融合タンパク質は、配列番号12又は配列番号16で表示されるアミノ酸配列、及び、配列番号14又は配列番号18で表示されるアミノ酸配列からなることを特徴とする請求項1に記載の薬学的組成物。
- 前記融合タンパク質は、配列番号20で表示されるアミノ酸配列及び配列番号22で表示されるアミノ酸配列からなることを特徴とする請求項1に記載の薬学的組成物。
- 前記融合は、リンカーペプチドによるものであることを特徴とする請求項1に記載の薬学的組成物。
- 前記眼疾患は、増殖硝子体網膜病症、黄斑変性、色素性網膜症、糖尿病性網膜症、脈絡膜血管新生、血管新生性緑内障、虚血性視神経障害、早熟児網膜症、未熟児網膜症、流行性角結膜炎、血管新生性虹彩疾患、厚水晶体繊維増殖症、アトピー性角膜炎、上角膜潤部角膜炎、位相編乾癬角膜炎、フリックテン性角結膜炎、強膜炎、及び糖尿性黄斑浮腫からなる群より選ばれたことを特徴とする請求項1〜6のうちいずれか一つに記載の薬学的組成物。
- (a)組織透過性ペプチドと抗血管内皮細胞成長因子が融合した融合タンパク質をコードする核酸配列を含む組換えベクターを宿主細胞にトランスフェクションする段階;
(b)前記細胞を培養する段階;及び
(c)前記細胞から融合タンパク質を回収する段階を含み、
前記組織透過性ペプチドが配列番号1〜7からなる群より選ばれたいずれか一つのアミノ酸配列を含み、
前記抗血管内皮細胞成長因子(anti-VEGF)が、ラニビズマブ(ranibizumab)、ベバシズマブ(bevacizumab)、アフリベルセプト(aflibercept)、コンバーセプト(Conbercept)、r84、CT01、DOM15-10-11、DOM15-26-593、CT-322、ESBA903、及びEPI-0030並びにこれらの変異体で構成された群から選ばれる、耐性克服及び効能が向上した抗血管内皮細胞成長因子を製造する方法。 - 前記融合は、リンカーペプチドによるものであることを特徴とする請求項8記載の方法。
- 組織透過性ペプチドと抗血管内皮細胞成長因子が融合した融合タンパク質を有効成分として含む眼疾患の治療用製剤を製造するための前記融合タンパク質の使用方法であって、
前記組織透過性ペプチドが配列番号1〜7からなる群より選ばれたいずれか一つのアミノ酸配列を含み、
前記抗血管内皮細胞成長因子(anti-VEGF)が、ラニビズマブ(ranibizumab)、ベバシズマブ(bevacizumab)、アフリベルセプト(aflibercept)、コンバーセプト(Conbercept)、r84、CT01、DOM15-10-11、DOM15-26-593、CT-322、ESBA903、及びEPI-0030並びにこれらの変異体で構成された群から選ばれる、方法。
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