JP6824594B2 - 合成遺伝子の設計方法 - Google Patents
合成遺伝子の設計方法 Download PDFInfo
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- JP6824594B2 JP6824594B2 JP2014185629A JP2014185629A JP6824594B2 JP 6824594 B2 JP6824594 B2 JP 6824594B2 JP 2014185629 A JP2014185629 A JP 2014185629A JP 2014185629 A JP2014185629 A JP 2014185629A JP 6824594 B2 JP6824594 B2 JP 6824594B2
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- recombinant
- gene
- mammalian cell
- luciferase
- protein
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12013—Oplophorus-luciferin 2-monooxygenase (1.13.12.13)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
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- Analytical Chemistry (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014185629A JP6824594B2 (ja) | 2014-09-11 | 2014-09-11 | 合成遺伝子の設計方法 |
| GB1515662.3A GB2535563B (en) | 2014-09-11 | 2015-09-04 | Design method for synthetic genes |
| US14/848,535 US9938539B2 (en) | 2014-09-11 | 2015-09-09 | Method for synthetic genes |
| US15/905,521 US10196650B2 (en) | 2014-09-11 | 2018-02-26 | Method for synthetic genes |
| US16/216,228 US11268106B2 (en) | 2014-09-11 | 2018-12-11 | Method for synthetic genes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014185629A JP6824594B2 (ja) | 2014-09-11 | 2014-09-11 | 合成遺伝子の設計方法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019148272A Division JP2019187453A (ja) | 2019-08-13 | 2019-08-13 | 合成遺伝子の設計方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016054715A JP2016054715A (ja) | 2016-04-21 |
| JP2016054715A5 JP2016054715A5 (enExample) | 2017-05-18 |
| JP6824594B2 true JP6824594B2 (ja) | 2021-02-03 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014185629A Expired - Fee Related JP6824594B2 (ja) | 2014-09-11 | 2014-09-11 | 合成遺伝子の設計方法 |
Country Status (3)
| Country | Link |
|---|---|
| US (3) | US9938539B2 (enExample) |
| JP (1) | JP6824594B2 (enExample) |
| GB (1) | GB2535563B (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10121100B2 (en) | 2016-12-20 | 2018-11-06 | Capital One Services, Llc | Two piece transaction card having fabric inlay |
| US20220308044A1 (en) * | 2019-06-21 | 2022-09-29 | University Of Utah Research Foundation | Novel live-cell assay for neuronal activity |
| CN112646838B (zh) * | 2021-01-06 | 2022-06-28 | 中国人民解放军军事科学院军事医学研究院 | 一种hspa13基因表达载体及其构建方法和应用 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5670356A (en) * | 1994-12-12 | 1997-09-23 | Promega Corporation | Modified luciferase |
| US5976796A (en) * | 1996-10-04 | 1999-11-02 | Loma Linda University | Construction and expression of renilla luciferase and green fluorescent protein fusion genes |
| WO1999049019A2 (en) * | 1998-03-27 | 1999-09-30 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
| HUP0201019A2 (en) * | 1999-04-26 | 2002-08-28 | Leuven K U Res & Dev | Synthetic gene for expressing active retroviral protein in eukaryotes |
| US6949368B2 (en) | 2001-01-30 | 2005-09-27 | The Trustees Of Princeton University | Compositions and methods for enhancing polynucleotide amplification reactions |
| SE0101519D0 (sv) | 2001-04-27 | 2001-04-27 | Astrazeneca Ab | Nucleic ACID |
| PT1857122E (pt) * | 2001-06-05 | 2011-03-07 | Curevac Gmbh | Arnm estabilizado com teor de g/c aumentado, codificando para um antigénio viral |
| DE102004037611B4 (de) | 2004-08-03 | 2013-10-02 | Geneart Ag | Induzierbare Genexpression |
| WO2006049777A2 (en) * | 2004-09-30 | 2006-05-11 | Trustees Of Dartmouth College | Nucleic acid sequences encoding luciferase for expression in filamentous fungi |
| KR20080100420A (ko) * | 2006-01-11 | 2008-11-18 | 악삼 에스피에이 | 발광성 줄기 세포들 및 이들의 용도 |
| US20100159449A1 (en) * | 2006-05-26 | 2010-06-24 | Noacell Science Co., Ltd. | Continuous Assay Method for Measuring Receptor- and Ion Channel-Mediated Calcium Response and Subsequent Gene Expression |
| US20100035287A1 (en) * | 2006-10-12 | 2010-02-11 | The General Hospital Corporation | Secreted Luciferase Fluorescent Protein Conjugate Nucleic Acid Construct and Uses Thereof |
| US20080305500A1 (en) * | 2007-06-08 | 2008-12-11 | Senomyx, Inc. | Novel cell-based assays for identifying enhancers or inhibitors of t1r taste receptors (t1r2/t1r3 sweet) and umami (t1r1/t1r3 umami) taste receptors |
| EP2019134A1 (en) | 2007-07-26 | 2009-01-28 | Vision 7 GmbH | Gene therapy of chronic granulomatous disease |
| US8450054B2 (en) * | 2008-10-10 | 2013-05-28 | Marker Gene Technologies, Inc. | Modified Luciola cruciata luciferase gene and protein |
| US7723502B2 (en) * | 2008-10-10 | 2010-05-25 | Marker Gene Technologies, Inc | Modified Luciola cruciata luciferase gene and protein |
| JP5304275B2 (ja) | 2009-01-29 | 2013-10-02 | Ｊｎｃ株式会社 | アポクライティン−ｉｉをコードするコドン最適化核酸およびその使用方法 |
| US20110081298A1 (en) * | 2009-10-01 | 2011-04-07 | Andrew Tsourkas | Ratiometric bioluminescent sensor for imagining oxidative stress |
| WO2011102178A1 (ja) | 2010-02-19 | 2011-08-25 | 独立行政法人産業技術総合研究所 | 改良されたレポーター遺伝子を用いる分析システム |
| DK2571998T3 (en) * | 2010-05-19 | 2017-09-25 | Icosagen Cell Factory Oü | METHOD AND KIT FOR IDENTIFICATION OF CHEMICAL COMPOUNDS MADE TO INHIBIT HUMAN PAPILLOMA VIRUS REPLICATION |
| KR20120107741A (ko) * | 2011-03-22 | 2012-10-04 | 한국생명공학연구원 | 패혈증 단백질 치료제의 효능 분석 및 스크리닝 방법 |
| KR101292894B1 (ko) * | 2011-04-26 | 2013-08-02 | 서울대학교산학협력단 | 루시퍼라아제 유전자를 과발현하는 형질전환 동물 및 이의 제조 방법 |
| US9547014B2 (en) * | 2011-06-10 | 2017-01-17 | Cornell University | Immobilized protein system for rapid and enhanced multiplexed diagnostics |
| WO2013056212A2 (en) | 2011-10-14 | 2013-04-18 | Phycal Llc | Chlorophyte genes for the optimization of productivity |
| AU2013242404B2 (en) * | 2012-03-27 | 2018-08-30 | CureVac SE | Artificial nucleic acid molecules for improved protein or peptide expression |
| JP6511720B2 (ja) * | 2013-02-28 | 2019-05-15 | Jnc株式会社 | コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法 |
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