GB2535563B - Design method for synthetic genes - Google Patents

Design method for synthetic genes Download PDF

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Publication number
GB2535563B
GB2535563B GB1515662.3A GB201515662A GB2535563B GB 2535563 B GB2535563 B GB 2535563B GB 201515662 A GB201515662 A GB 201515662A GB 2535563 B GB2535563 B GB 2535563B
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United Kingdom
Prior art keywords
design method
synthetic genes
genes
synthetic
design
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB1515662.3A
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English (en)
Other versions
GB2535563A (en
GB201515662D0 (en
Inventor
Inouye Satoshi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
JNC Corp
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JNC Corp
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Publication of GB2535563A publication Critical patent/GB2535563A/en
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Publication of GB2535563B publication Critical patent/GB2535563B/en
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12013—Oplophorus-luciferin 2-monooxygenase (1.13.12.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
GB1515662.3A 2014-09-11 2015-09-04 Design method for synthetic genes Expired - Fee Related GB2535563B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2014185629A JP6824594B2 (ja) 2014-09-11 2014-09-11 合成遺伝子の設計方法

Publications (3)

Publication Number Publication Date
GB201515662D0 GB201515662D0 (en) 2015-10-21
GB2535563A GB2535563A (en) 2016-08-24
GB2535563B true GB2535563B (en) 2020-10-28

Family

ID=54345750

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1515662.3A Expired - Fee Related GB2535563B (en) 2014-09-11 2015-09-04 Design method for synthetic genes

Country Status (3)

Country Link
US (3) US9938539B2 (enExample)
JP (1) JP6824594B2 (enExample)
GB (1) GB2535563B (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10121100B2 (en) 2016-12-20 2018-11-06 Capital One Services, Llc Two piece transaction card having fabric inlay
US20220308044A1 (en) * 2019-06-21 2022-09-29 University Of Utah Research Foundation Novel live-cell assay for neuronal activity
CN112646838B (zh) * 2021-01-06 2022-06-28 中国人民解放军军事科学院军事医学研究院 一种hspa13基因表达载体及其构建方法和应用
CN117467687B (zh) * 2021-10-18 2026-07-14 上海交通大学 增强表达安丝菌素体内靶标蛋白apasm_5765的高产安丝菌素方法

Citations (11)

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US5670356A (en) * 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
WO2000065076A2 (en) * 1999-04-26 2000-11-02 K.U. Leuven Research & Development Synthetic gene for expressing active retroviral protein in eukaryotes
WO2007080622A2 (en) * 2006-01-11 2007-07-19 Axxam S.P.A. Luminescent stem cells and uses thereof
US20080305500A1 (en) * 2007-06-08 2008-12-11 Senomyx, Inc. Novel cell-based assays for identifying enhancers or inhibitors of t1r taste receptors (t1r2/t1r3 sweet) and umami (t1r1/t1r3 umami) taste receptors
EP2036986A1 (en) * 2006-05-26 2009-03-18 Noacell Science Co., Ltd. Continuous assay method for measuring receptor- and ion channel-mediated calcium response and subsequent gene expression
US20100093029A1 (en) * 2008-10-10 2010-04-15 Coleman Daniel J Modified luciola cruciata luciferase gene and protein
US20110081298A1 (en) * 2009-10-01 2011-04-07 Andrew Tsourkas Ratiometric bioluminescent sensor for imagining oxidative stress
US20120028257A1 (en) * 2008-10-10 2012-02-02 Marker Gene Technologies, Inc. Modified luciola cruciata luciferase gene and protein
WO2012170998A1 (en) * 2011-06-10 2012-12-13 Cornell University Immobilized protein system for rapid and enhanced multiplexed diagnostics
US20140088292A1 (en) * 2011-03-22 2014-03-27 Korea Advanced Institute Of Science And Technology Water-Soluble Polypeptides Comprised of Repeat Modules, Method for Preparing the Same and Method for a Target-Specific Polypeptide and Analysis of Biological Activity Thereof
US20140242574A1 (en) * 2013-02-28 2014-08-28 Jnc Corporation Codon-optimized gene for mutated shrimp luciferase and method for use thereof

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US5976796A (en) * 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
AU765703B2 (en) * 1998-03-27 2003-09-25 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US6949368B2 (en) 2001-01-30 2005-09-27 The Trustees Of Princeton University Compositions and methods for enhancing polynucleotide amplification reactions
SE0101519D0 (sv) 2001-04-27 2001-04-27 Astrazeneca Ab Nucleic ACID
ATE291925T1 (de) * 2001-06-05 2005-04-15 Curevac Gmbh Stabilisierte mrna mit erhöhtem g/c-gehalt und optimierter codon usage für die gentherapie
DE102004037611B4 (de) 2004-08-03 2013-10-02 Geneart Ag Induzierbare Genexpression
WO2006049777A2 (en) * 2004-09-30 2006-05-11 Trustees Of Dartmouth College Nucleic acid sequences encoding luciferase for expression in filamentous fungi
WO2008066608A2 (en) 2006-10-12 2008-06-05 The General Hospital Corporation A secreted luciferase fluorescent protein conjugate nucleic acid construct and uses thereof
EP2019134A1 (en) 2007-07-26 2009-01-28 Vision 7 GmbH Gene therapy of chronic granulomatous disease
JP5304275B2 (ja) 2009-01-29 2013-10-02 Ｊｎｃ株式会社 アポクライティン−ｉｉをコードするコドン最適化核酸およびその使用方法
WO2011102178A1 (ja) 2010-02-19 2011-08-25 独立行政法人産業技術総合研究所 改良されたレポーター遺伝子を用いる分析システム
ES2643610T3 (es) * 2010-05-19 2017-11-23 Icosagen Cell Factory Oü Método y kit para identificar compuestos capaces de inhibir la replicación del virus del papiloma humano
KR101292894B1 (ko) * 2011-04-26 2013-08-02 서울대학교산학협력단 루시퍼라아제 유전자를 과발현하는 형질전환 동물 및 이의 제조 방법
WO2013056212A2 (en) 2011-10-14 2013-04-18 Phycal Llc Chlorophyte genes for the optimization of productivity
RU2658490C2 (ru) * 2012-03-27 2018-06-21 Кьюрвак Аг Искусственные молекулы нуклеиновых кислот для улучшенной экспрессии белков или пептидов

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5670356A (en) * 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
WO2000065076A2 (en) * 1999-04-26 2000-11-02 K.U. Leuven Research & Development Synthetic gene for expressing active retroviral protein in eukaryotes
WO2007080622A2 (en) * 2006-01-11 2007-07-19 Axxam S.P.A. Luminescent stem cells and uses thereof
EP2036986A1 (en) * 2006-05-26 2009-03-18 Noacell Science Co., Ltd. Continuous assay method for measuring receptor- and ion channel-mediated calcium response and subsequent gene expression
US20080305500A1 (en) * 2007-06-08 2008-12-11 Senomyx, Inc. Novel cell-based assays for identifying enhancers or inhibitors of t1r taste receptors (t1r2/t1r3 sweet) and umami (t1r1/t1r3 umami) taste receptors
US20100093029A1 (en) * 2008-10-10 2010-04-15 Coleman Daniel J Modified luciola cruciata luciferase gene and protein
US20120028257A1 (en) * 2008-10-10 2012-02-02 Marker Gene Technologies, Inc. Modified luciola cruciata luciferase gene and protein
US20110081298A1 (en) * 2009-10-01 2011-04-07 Andrew Tsourkas Ratiometric bioluminescent sensor for imagining oxidative stress
US20140088292A1 (en) * 2011-03-22 2014-03-27 Korea Advanced Institute Of Science And Technology Water-Soluble Polypeptides Comprised of Repeat Modules, Method for Preparing the Same and Method for a Target-Specific Polypeptide and Analysis of Biological Activity Thereof
WO2012170998A1 (en) * 2011-06-10 2012-12-13 Cornell University Immobilized protein system for rapid and enhanced multiplexed diagnostics
US20140242574A1 (en) * 2013-02-28 2014-08-28 Jnc Corporation Codon-optimized gene for mutated shrimp luciferase and method for use thereof

Also Published As

Publication number Publication date
US11268106B2 (en) 2022-03-08
US9938539B2 (en) 2018-04-10
US20160076052A1 (en) 2016-03-17
US10196650B2 (en) 2019-02-05
GB2535563A (en) 2016-08-24
GB201515662D0 (en) 2015-10-21
US20180171356A1 (en) 2018-06-21
JP6824594B2 (ja) 2021-02-03
US20190100770A1 (en) 2019-04-04
JP2016054715A (ja) 2016-04-21

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PCNP Patent ceased through non-payment of renewal fee

Effective date: 20230904