JP6740222B2 - 互いに消光する蛍光標識とのナノポアを基礎にしたポリマー分析 - Google Patents

互いに消光する蛍光標識とのナノポアを基礎にしたポリマー分析 Download PDF

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JP6740222B2
JP6740222B2 JP2017518524A JP2017518524A JP6740222B2 JP 6740222 B2 JP6740222 B2 JP 6740222B2 JP 2017518524 A JP2017518524 A JP 2017518524A JP 2017518524 A JP2017518524 A JP 2017518524A JP 6740222 B2 JP6740222 B2 JP 6740222B2
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nanopore
fluorescent
fret
nanopores
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JP2017531431A5 (enExample
JP2017531431A (ja
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マーティン フーバー，
マーティン フーバー，
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クアンタポール， インコーポレイテッド
クアンタポール， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2017518524A 2014-10-10 2015-10-08 互いに消光する蛍光標識とのナノポアを基礎にしたポリマー分析 Active JP6740222B2 (ja)

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US201462062256P 2014-10-10 2014-10-10
US62/062,256 2014-10-10
PCT/US2015/054756 WO2016057829A1 (en) 2014-10-10 2015-10-08 Nanopore-based polymer analysis with mutually-quenching fluorescent labels

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JP2017531431A JP2017531431A (ja) 2017-10-26
JP2017531431A5 JP2017531431A5 (enExample) 2018-08-30
JP6740222B2 true JP6740222B2 (ja) 2020-08-12

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JP2018133008A Pending JP2018157835A (ja) 2014-10-10 2018-07-13 互いに消光する蛍光標識とのナノポアを基礎にしたポリマー分析

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US (4) US9885079B2 (enExample)
EP (1) EP3204519B1 (enExample)
JP (2) JP6740222B2 (enExample)
CN (1) CN107109472B (enExample)
AU (1) AU2015330841B2 (enExample)
CA (1) CA2963604C (enExample)
ES (1) ES2789000T3 (enExample)
WO (1) WO2016057829A1 (enExample)

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WO2017180319A1 (en) * 2016-04-14 2017-10-19 Quantapore, Inc. Mixed optical signals in polymer analysis with nanopores
CN109477813A (zh) * 2016-07-05 2019-03-15 昆塔波尔公司 基于光学的纳米孔测序
WO2018034807A1 (en) 2016-08-19 2018-02-22 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents
JP2019528776A (ja) * 2016-10-10 2019-10-17 クアンタポール， インコーポレイテッド 光学に基づくナノポア配列決定における蛍光標識組成物の制御
LU100392B1 (en) * 2017-07-27 2019-02-12 Wisconsin Alumni Res Foundation Warf A method to determine the throughput speed of a pore
CN110243728B (zh) * 2018-03-07 2023-02-10 中国石油天然气股份有限公司 一种乳液液滴粒径分布的确定方法及用于标定乳液的化合物
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
EP4172358A1 (en) * 2020-06-30 2023-05-03 Dimensiongen Devices and methods for genomic structural analysis
CN113533275B (zh) * 2021-06-30 2023-11-28 东南大学 固态纳米孔-荧光共振能量转移复合检测方法及系统

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