ES2789000T3 - Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente - Google Patents
Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente Download PDFInfo
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- ES2789000T3 ES2789000T3 ES15849196T ES15849196T ES2789000T3 ES 2789000 T3 ES2789000 T3 ES 2789000T3 ES 15849196 T ES15849196 T ES 15849196T ES 15849196 T ES15849196 T ES 15849196T ES 2789000 T3 ES2789000 T3 ES 2789000T3
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
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| WO2017123647A1 (en) | 2016-01-15 | 2017-07-20 | Quantapore, Inc. | Optically-based nanopore analysis with reduced background |
| WO2017180319A1 (en) * | 2016-04-14 | 2017-10-19 | Quantapore, Inc. | Mixed optical signals in polymer analysis with nanopores |
| CN109477813A (zh) * | 2016-07-05 | 2019-03-15 | 昆塔波尔公司 | 基于光学的纳米孔测序 |
| WO2018034807A1 (en) | 2016-08-19 | 2018-02-22 | Quantapore, Inc. | Optically-based nanopore sequencing using quenching agents |
| JP2019528776A (ja) * | 2016-10-10 | 2019-10-17 | クアンタポール, インコーポレイテッド | 光学に基づくナノポア配列決定における蛍光標識組成物の制御 |
| LU100392B1 (en) * | 2017-07-27 | 2019-02-12 | Wisconsin Alumni Res Foundation Warf | A method to determine the throughput speed of a pore |
| CN110243728B (zh) * | 2018-03-07 | 2023-02-10 | 中国石油天然气股份有限公司 | 一种乳液液滴粒径分布的确定方法及用于标定乳液的化合物 |
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Family Cites Families (220)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH614781A5 (enExample) | 1977-06-27 | 1979-12-14 | Contraves Ag | |
| US5821058A (en) | 1984-01-16 | 1998-10-13 | California Institute Of Technology | Automated DNA sequencing technique |
| US4962037A (en) | 1987-10-07 | 1990-10-09 | United States Of America | Method for rapid base sequencing in DNA and RNA |
| US5131755A (en) | 1988-02-19 | 1992-07-21 | Chadwick Curt H | Automatic high speed optical inspection system |
| US5126022A (en) | 1990-02-28 | 1992-06-30 | Soane Tecnologies, Inc. | Method and device for moving molecules by the application of a plurality of electrical fields |
| US6054034A (en) | 1990-02-28 | 2000-04-25 | Aclara Biosciences, Inc. | Acrylic microchannels and their use in electrophoretic applications |
| JP3123249B2 (ja) | 1991-09-10 | 2001-01-09 | 株式会社日立製作所 | Dna分子の長さ計測方法および計測装置 |
| US5405747A (en) | 1991-09-25 | 1995-04-11 | The Regents Of The University Of California Office Of Technology Transfer | Method for rapid base sequencing in DNA and RNA with two base labeling |
| DE69322266T2 (de) | 1992-04-03 | 1999-06-02 | Perkin-Elmer Corp., Foster City, Calif. | Proben zusammensetzung und verfahren |
| US5470705A (en) | 1992-04-03 | 1995-11-28 | Applied Biosystems, Inc. | Probe composition containing a binding domain and polymer chain and methods of use |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5498392A (en) | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| US5387926A (en) | 1992-06-30 | 1995-02-07 | California Institute Of Technology | High speed digital framing camera |
| US5798042A (en) | 1994-03-07 | 1998-08-25 | Regents Of The University Of California | Microfabricated filter with specially constructed channel walls, and containment well and capsule constructed with such filters |
| US6001229A (en) | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6794127B1 (en) | 1997-06-16 | 2004-09-21 | Diversa Corporation | Capillary array-based sample screening |
| AU2804597A (en) | 1996-04-15 | 1997-11-07 | University Of Alberta | Synthesis of fluorophore-labeled dna |
| US6399023B1 (en) | 1996-04-16 | 2002-06-04 | Caliper Technologies Corp. | Analytical system and method |
| US5945526A (en) | 1996-05-03 | 1999-08-31 | Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| US6136543A (en) | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
| ATE273381T1 (de) | 1997-02-12 | 2004-08-15 | Eugene Y Chan | Verfahren zur analyse von polymeren |
| DE19714221A1 (de) | 1997-04-07 | 1998-10-08 | Zeiss Carl Fa | Konfokales Mikroskop mit einem motorischen Scanningtisch |
| CZ297165B6 (cs) | 1997-04-21 | 2006-09-13 | Randox Laboratories Ltd. A British Company Of Ardmore | Zpusob výroby zarízení s pevnou fází k provádení multianalytních rozboru |
| WO1999005167A1 (en) | 1997-07-25 | 1999-02-04 | University Of Massachusetts | Designed protein pores as components for biosensors |
| CA2306126A1 (en) | 1997-10-15 | 1999-04-22 | Aclara Biosciences, Inc. | Laminate microstructure device and method for making same |
| AU1603199A (en) | 1997-12-03 | 1999-06-16 | Curagen Corporation | Methods and devices for measuring differential gene expression |
| US20020144905A1 (en) | 1997-12-17 | 2002-10-10 | Christian Schmidt | Sample positioning and analysis system |
| US7244349B2 (en) | 1997-12-17 | 2007-07-17 | Molecular Devices Corporation | Multiaperture sample positioning and analysis system |
| ATE205300T1 (de) | 1997-12-17 | 2001-09-15 | Ecole Polytech | Positionierung und elektrophysiologische charakterisierung einzelner zellen und rekonstituierter membransysteme auf mikrostrukturierten trägern |
| EP1042009B1 (en) | 1997-12-23 | 2001-07-04 | Steris Corporation | Antimicrobial composition delivery system with an integrated filter |
| US6265163B1 (en) | 1998-01-09 | 2001-07-24 | Lynx Therapeutics, Inc. | Solid phase selection of differentially expressed genes |
| DE19804372A1 (de) | 1998-02-04 | 1999-08-05 | Michael W Dr Dr Dahm | Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits |
| GB9805301D0 (en) | 1998-03-12 | 1998-05-06 | Imperial College | Detector |
| US6306589B1 (en) | 1998-05-27 | 2001-10-23 | Vysis, Inc. | Biological assays for analyte detection |
| US6504943B1 (en) | 1998-07-20 | 2003-01-07 | Sandia Corporation | Information-efficient spectral imaging sensor |
| US6447724B1 (en) | 1998-08-11 | 2002-09-10 | Caliper Technologies Corp. | DNA sequencing using multiple fluorescent labels being distinguishable by their decay times |
| US6790671B1 (en) | 1998-08-13 | 2004-09-14 | Princeton University | Optically characterizing polymers |
| US6210896B1 (en) | 1998-08-13 | 2001-04-03 | Us Genomics | Molecular motors |
| US6263286B1 (en) | 1998-08-13 | 2001-07-17 | U.S. Genomics, Inc. | Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer |
| US6906749B1 (en) | 1998-09-16 | 2005-06-14 | Dalsa, Inc. | CMOS TDI image sensor |
| EP0990903B1 (en) | 1998-09-18 | 2003-03-12 | Massachusetts Institute Of Technology | Biological applications of semiconductor nanocrystals |
| US6251303B1 (en) | 1998-09-18 | 2001-06-26 | Massachusetts Institute Of Technology | Water-soluble fluorescent nanocrystals |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
| US6252303B1 (en) | 1998-12-02 | 2001-06-26 | Advanced Micro Devices, Inc. | Intergration of low-K SiOF as inter-layer dielectric |
| US6465193B2 (en) | 1998-12-11 | 2002-10-15 | The Regents Of The University Of California | Targeted molecular bar codes and methods for using the same |
| FR2788008B1 (fr) | 1998-12-30 | 2001-03-23 | Inst Curie | Milieu thermosensible pour la separation electrocinetique d'especes au sein d'un canal de separation |
| US6455682B1 (en) | 1999-01-20 | 2002-09-24 | Northwestern University | DNA mobility modifier |
| US6608682B2 (en) | 1999-01-25 | 2003-08-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6975400B2 (en) | 1999-01-25 | 2005-12-13 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6473176B2 (en) | 1999-01-25 | 2002-10-29 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6671044B2 (en) | 1999-01-25 | 2003-12-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells in broad flat flow |
| US6249341B1 (en) | 1999-01-25 | 2001-06-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| JP2003527075A (ja) | 1999-03-25 | 2003-09-16 | ハイセック,インコーポレーテッド | ハイブリダイゼーションによる配列分析のための、溶液ベースの方法 |
| US20040214221A1 (en) | 1999-05-07 | 2004-10-28 | Klaus Muehlegger | High density labeling of DNA with modified or "chromophore" carrying nucleotides and DNA polymerases used |
| US7122152B2 (en) | 1999-05-10 | 2006-10-17 | University Of Florida | Spatiotemporal and geometric optimization of sensor arrays for detecting analytes fluids |
| EP1192447A2 (en) | 1999-05-12 | 2002-04-03 | Aclara BioSciences, Inc. | Multiplexed fluorescent detection in microfluidic devices |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| JP2003502166A (ja) | 1999-06-22 | 2003-01-21 | プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ | 固体状態の次元的特徴の制御 |
| US8206568B2 (en) | 1999-06-22 | 2012-06-26 | President And Fellows Of Harvard College | Material deposition techniques for control of solid state aperture surface properties |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US6982146B1 (en) | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
| AU7105300A (en) | 1999-09-03 | 2001-04-10 | Lifebeam Technologies, Inc. | Optical system for rapid polymer analysis |
| DE60025739T2 (de) | 1999-09-07 | 2006-08-31 | The Regents Of The University Of California, Oakland | Verfahren um die anwesenheit von doppelsträngiger dns in einer probe nachzuweisen |
| US6335420B1 (en) | 1999-11-22 | 2002-01-01 | Ibc Advanced Technologies, Inc. | Polyamide ligand-containing polymeric resins and methods of using the same for removing, separating and/or concentrating desired metal ions from solutions |
| WO2001055454A1 (en) | 2000-01-28 | 2001-08-02 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| AU2001241474A1 (en) | 2000-02-11 | 2001-08-20 | The Texas A And M University System | Biosensor compositions and methods of use |
| US20060292041A1 (en) | 2000-03-23 | 2006-12-28 | Dugas Matthew P | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US6616895B2 (en) | 2000-03-23 | 2003-09-09 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US6917726B2 (en) | 2001-09-27 | 2005-07-12 | Cornell Research Foundation, Inc. | Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes |
| AU2001269109A1 (en) | 2000-06-30 | 2002-01-14 | Gnothis Holding Sa | Single molecule sequencing method |
| JP2004513619A (ja) | 2000-07-07 | 2004-05-13 | ヴィジゲン バイオテクノロジーズ インコーポレイテッド | リアルタイム配列決定 |
| US6583865B2 (en) | 2000-08-25 | 2003-06-24 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
| US6934408B2 (en) | 2000-08-25 | 2005-08-23 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
| US6608680B2 (en) | 2000-08-25 | 2003-08-19 | Amnis Corporation | TDI imaging system for kinetic studies |
| AU2001293232A1 (en) | 2000-08-29 | 2002-03-13 | The Rockefeller University | Methods employing fluorescence quenching by metal surfaces |
| US6939451B2 (en) | 2000-09-19 | 2005-09-06 | Aclara Biosciences, Inc. | Microfluidic chip having integrated electrodes |
| AU2002239284A1 (en) | 2000-11-27 | 2002-06-03 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US6998251B2 (en) | 2001-01-12 | 2006-02-14 | Syngenta Participations Ag | Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics |
| WO2002081729A2 (en) | 2001-04-06 | 2002-10-17 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
| US6743905B2 (en) | 2001-04-16 | 2004-06-01 | Applera Corporation | Mobility-modified nucleobase polymers and methods of using same |
| CN1287587C (zh) | 2001-05-15 | 2006-11-29 | 株式会社荏原制作所 | Tdi检测装置、应用该装置的电子束设备以及应用该电子束设备的半导体器件制造方法 |
| WO2002094846A2 (en) | 2001-05-22 | 2002-11-28 | Parallel Synthesis Technologies, Inc. | Method for in situ, on-chip chemical synthesis |
| WO2002103335A1 (en) | 2001-06-18 | 2002-12-27 | Amnis Corporation | Spectral deconvolution of fluorescent markers |
| US7009163B2 (en) | 2001-06-22 | 2006-03-07 | Orbotech Ltd. | High-sensitivity optical scanning using memory integration |
| JP2005508493A (ja) | 2001-06-28 | 2005-03-31 | アドヴァンスト リサーチ アンド テクノロジー インスティテュート、インコーポレイティッド | 多色量子ドット標識ビーズおよびそのコンジュゲートの製造方法 |
| SG169225A1 (en) | 2001-07-25 | 2011-03-30 | Univ Princeton | Nanochannel arrays and their preparation and use for high throughput macromolecular analysis |
| US6856390B2 (en) | 2001-07-25 | 2005-02-15 | Applera Corporation | Time-delay integration in electrophoretic detection systems |
| US7280207B2 (en) | 2001-07-25 | 2007-10-09 | Applera Corporation | Time-delay integration in a flow cytometry system |
| US6627433B2 (en) | 2001-08-24 | 2003-09-30 | Applera Corporation | Multi-channel analyte-separation device employing side-entry excitation |
| US20080288178A1 (en) | 2001-08-24 | 2008-11-20 | Applera Corporation | Sequencing system with memory |
| US6852492B2 (en) | 2001-09-24 | 2005-02-08 | Intel Corporation | Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication |
| US6706203B2 (en) | 2001-10-30 | 2004-03-16 | Agilent Technologies, Inc. | Adjustable nanopore, nanotome, and nanotweezer |
| DE10154291B4 (de) | 2001-11-05 | 2005-05-19 | Hain Lifescience Gmbh | Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes |
| DE10162535A1 (de) | 2001-12-19 | 2003-07-03 | Gnothis Holding Sa Ecublens | Sequenzierung über Lochmembranen |
| US7008547B2 (en) | 2002-03-14 | 2006-03-07 | Sarnoff Corporation | Solid phase sensors |
| JP3752466B2 (ja) | 2002-04-24 | 2006-03-08 | 株式会社日立製作所 | 遺伝子検査方法 |
| US8278055B2 (en) | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
| US7744816B2 (en) | 2002-05-01 | 2010-06-29 | Intel Corporation | Methods and device for biomolecule characterization |
| AU2003245272A1 (en) | 2002-05-10 | 2003-11-11 | The Texas A And M University System | Stochastic sensing through covalent interactions |
| US20050019784A1 (en) | 2002-05-20 | 2005-01-27 | Xing Su | Method and apparatus for nucleic acid sequencing and identification |
| US7005264B2 (en) | 2002-05-20 | 2006-02-28 | Intel Corporation | Method and apparatus for nucleic acid sequencing and identification |
| US6952651B2 (en) | 2002-06-17 | 2005-10-04 | Intel Corporation | Methods and apparatus for nucleic acid sequencing by signal stretching and data integration |
| TWI234584B (en) | 2002-08-16 | 2005-06-21 | Univ Nat Taiwan Normal | Nucleic acid sequencing method |
| US7651599B2 (en) | 2002-09-25 | 2010-01-26 | Ge Healthcare (Sv) Corp. | High density fluidic chip design and method of sample injection |
| AU2003301244A1 (en) | 2002-10-15 | 2004-05-04 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| CN1161613C (zh) | 2002-10-22 | 2004-08-11 | 中国人民解放军南京军区联勤部军事医学研究所 | 免疫细胞微流体阵列 |
| US7390457B2 (en) | 2002-10-31 | 2008-06-24 | Agilent Technologies, Inc. | Integrated microfluidic array device |
| US20040137158A1 (en) | 2003-01-15 | 2004-07-15 | Kools Jacques Constant Stefan | Method for preparing a noble metal surface |
| US7410564B2 (en) | 2003-01-27 | 2008-08-12 | Agilent Technologies, Inc. | Apparatus and method for biopolymer identification during translocation through a nanopore |
| CA2513535C (en) | 2003-01-29 | 2012-06-12 | 454 Corporation | Bead emulsion nucleic acid amplification |
| JP2006526777A (ja) | 2003-02-28 | 2006-11-24 | ブラウン ユニバーシティー | ナノ細孔、その使用方法、その作成方法、及びそれを使用して生体分子を特徴付ける方法 |
| US7250115B2 (en) | 2003-06-12 | 2007-07-31 | Agilent Technologies, Inc | Nanopore with resonant tunneling electrodes |
| US7444053B2 (en) | 2003-06-16 | 2008-10-28 | The Regents Of The University Of California | Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity |
| US7248771B2 (en) | 2003-06-16 | 2007-07-24 | Brigham Young University | Integrated sensor with electrical and optical single molecule sensitivity |
| US7846738B2 (en) | 2003-08-15 | 2010-12-07 | President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7075161B2 (en) | 2003-10-23 | 2006-07-11 | Agilent Technologies, Inc. | Apparatus and method for making a low capacitance artificial nanopore |
| US20050095599A1 (en) | 2003-10-30 | 2005-05-05 | Pittaro Richard J. | Detection and identification of biopolymers using fluorescence quenching |
| US20050136408A1 (en) | 2003-12-19 | 2005-06-23 | May Tom-Moy | Methods and systems for characterizing a polymer |
| US7271896B2 (en) | 2003-12-29 | 2007-09-18 | Intel Corporation | Detection of biomolecules using porous biosensors and raman spectroscopy |
| US20050164211A1 (en) | 2004-01-22 | 2005-07-28 | Hannah Eric C. | Carbon nanotube molecular labels |
| US20080261204A1 (en) | 2004-01-23 | 2008-10-23 | Lingvitae As | Polynucleotide Ligation Reactions |
| US20050186576A1 (en) | 2004-02-19 | 2005-08-25 | Intel Corporation | Polymer sequencing using selectively labeled monomers and data integration |
| US7279337B2 (en) | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US20050227239A1 (en) | 2004-04-08 | 2005-10-13 | Joyce Timothy H | Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes |
| US20070264623A1 (en) | 2004-06-15 | 2007-11-15 | President And Fellows Of Harvard College | Nanosensors |
| US20060019259A1 (en) | 2004-07-22 | 2006-01-26 | Joyce Timothy H | Characterization of biopolymers by resonance tunneling and fluorescence quenching |
| GB2431013B (en) | 2004-07-23 | 2008-05-21 | Electronic Bio Sciences Llc | Method and apparatus for sensing a time varying current passing through an ion channel |
| US20070054276A1 (en) | 2004-08-12 | 2007-03-08 | Sampson Jeffrey R | Polynucleotide analysis and methods of using nanopores |
| EP1784754A4 (en) | 2004-08-13 | 2009-05-27 | Harvard College | OPTI-NANOPORE DNA READING PLATFORM WITH ULTRAHOLE THROUGHPUT |
| WO2006044078A2 (en) | 2004-09-17 | 2006-04-27 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| US20080025875A1 (en) | 2004-09-29 | 2008-01-31 | Martin Charles R | Chemical, Particle, and Biosensing with Nanotechnology |
| US7371533B2 (en) | 2004-10-05 | 2008-05-13 | University Of Ottawa | Methods for separation of polymeric compounds |
| WO2006052882A1 (en) | 2004-11-09 | 2006-05-18 | President And Fellows Of Harvard College | Formation of eddies in constrained fluidic channels and uses thereof |
| US7609309B2 (en) | 2004-11-18 | 2009-10-27 | Kla-Tencor Technologies Corporation | Continuous clocking of TDI sensors |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US20060210995A1 (en) | 2005-03-15 | 2006-09-21 | Joyce Timothy H | Nanopore analysis systems and methods of using nanopore devices |
| CN101203740B (zh) | 2005-04-06 | 2011-03-23 | 哈佛大学校长及研究员协会 | 用碳纳米管控制的分子鉴定 |
| US20060231419A1 (en) | 2005-04-15 | 2006-10-19 | Barth Philip W | Molecular resonant tunneling sensor and methods of fabricating and using the same |
| WO2006116683A1 (en) | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanostructure enhanced luminescence |
| EP1721657A1 (en) | 2005-05-13 | 2006-11-15 | SONY DEUTSCHLAND GmbH | A method of fabricating a polymeric membrane having at least one pore |
| JP2007006720A (ja) | 2005-06-28 | 2007-01-18 | Toshiba Corp | 個体識別方法、並びに個体識別検査のためのアレイ、装置及びシステム |
| US20090148348A1 (en) | 2005-08-11 | 2009-06-11 | Eksigent Technologies, Llc | Plastic surfaces and apparatuses for reduced adsorption of solutes and methods of preparing the same |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| WO2007041340A2 (en) | 2005-09-30 | 2007-04-12 | The Trustees Of Columbia University In The City Of New York | Microfluidic cells with parallel arrays of individual dna molecules |
| WO2007041621A2 (en) | 2005-10-03 | 2007-04-12 | Xingsheng Sean Ling | Hybridization assisted nanopore sequencing |
| KR100721430B1 (ko) | 2005-10-12 | 2007-05-23 | 학교법인 포항공과대학교 | 나노다공성 멤브레인 및 이의 제조방법 |
| US7397232B2 (en) | 2005-10-21 | 2008-07-08 | The University Of Akron | Coulter counter having a plurality of channels |
| US7835870B2 (en) | 2005-11-01 | 2010-11-16 | Georgia Institute Of Technology | Methods and systems for evaluating the length of elongated elements |
| EP1954838B1 (en) | 2005-11-14 | 2014-02-26 | Life Technologies Corporation | Coded molecules for detecting target analytes |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| DE602006016984D1 (de) | 2005-12-12 | 2010-10-28 | Us Gov Health & Human Serv | Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren |
| EP1987275A1 (en) | 2006-02-13 | 2008-11-05 | Koninklijke Philips Electronics N.V. | Microfluidic device for molecular diagnostic applications |
| US20070224613A1 (en) | 2006-02-18 | 2007-09-27 | Strathmann Michael P | Massively Multiplexed Sequencing |
| US20070202008A1 (en) | 2006-02-28 | 2007-08-30 | Schembri Carol T | Systems and methods of lipoprotein size fraction assaying |
| US20070215472A1 (en) | 2006-03-15 | 2007-09-20 | Slater Gary W | Electroosmotic flow for end labelled free solution electrophoresis |
| US20070218494A1 (en) | 2006-03-17 | 2007-09-20 | Gary Slater | Branched polymer lables as drag-tags in free solution electrophoresis |
| US7777505B2 (en) | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
| US7849581B2 (en) | 2006-05-05 | 2010-12-14 | University Of Utah Research Foundation | Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof |
| JP2009543058A (ja) | 2006-06-30 | 2009-12-03 | アプライド バイオシステムズ, エルエルシー | 結合相互作用を分析する方法 |
| US7553730B2 (en) | 2006-07-14 | 2009-06-30 | Agilent Technologies, Inc. | Methods of fabrication employing nanoscale mandrels |
| US20080032290A1 (en) | 2006-08-03 | 2008-02-07 | Young James E | Nanopore flow cells |
| ES2404665T3 (es) | 2006-09-06 | 2013-05-28 | Medical Research Council | Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante |
| WO2008049795A1 (en) | 2006-10-23 | 2008-05-02 | Flexgen Bv | Method and system for calibrating laser focus and position in micro-arrays |
| US7883869B2 (en) | 2006-12-01 | 2011-02-08 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| US7544578B2 (en) | 2007-01-03 | 2009-06-09 | International Business Machines Corporation | Structure and method for stochastic integrated circuit personalization |
| US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
| WO2008103848A1 (en) | 2007-02-21 | 2008-08-28 | Invitrogen Corporation | Materials and methods for single molecule nucleic acid sequencing |
| US20080254995A1 (en) | 2007-02-27 | 2008-10-16 | Drexel University | Nanopore arrays and sequencing devices and methods thereof |
| AU2008236694B2 (en) | 2007-04-04 | 2014-01-23 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| EP2158476B8 (en) | 2007-05-08 | 2019-10-09 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| CA2689626C (en) | 2007-06-06 | 2016-10-25 | Pacific Biosciences Of California, Inc. | Methods and processes for calling bases in sequence by incorporation methods |
| DK2171088T3 (en) | 2007-06-19 | 2016-01-25 | Stratos Genomics Inc | Nucleic acid sequencing in a high yield by expansion |
| GB0713143D0 (en) | 2007-07-06 | 2007-08-15 | Ucl Business Plc | Nucleic acid detection method |
| GB0717150D0 (en) | 2007-09-04 | 2007-10-17 | Univ Warwick | Apparatus and method |
| EP2205765B1 (en) | 2007-10-02 | 2012-09-12 | President and Fellows of Harvard College | Capture, recapture, and trapping of molecules with a nanopore |
| GB0721340D0 (en) | 2007-10-30 | 2007-12-12 | Isis Innovation | Polymerase-based single-molecule DNA sequencing |
| US8852864B2 (en) | 2008-01-17 | 2014-10-07 | Sequenom Inc. | Methods and compositions for the analysis of nucleic acids |
| GB2473333B (en) | 2008-02-28 | 2011-08-24 | Electronic Bio Sciences Llc | System and method to improve sequencing accuracy of a polymer |
| US8143030B2 (en) | 2008-09-24 | 2012-03-27 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| CN102084001B (zh) | 2008-03-28 | 2015-03-18 | 加利福尼亚太平洋生物科学股份有限公司 | 用于核酸测序的组合物和方法 |
| EP2279268A4 (en) | 2008-04-04 | 2011-12-07 | Life Technologies Corp | SCANNING SYSTEM AND METHOD OF IMAGING AND SEQUENCING |
| US20110172404A1 (en) | 2008-05-19 | 2011-07-14 | Cornell University | Self-Assembly of Nanoparticles Through Nuclei Acid Engineering |
| US8013300B2 (en) | 2008-06-20 | 2011-09-06 | Carl Zeiss Nts, Llc | Sample decontamination |
| US8628919B2 (en) | 2008-06-30 | 2014-01-14 | Bionano Genomics, Inc. | Methods and devices for single-molecule whole genome analysis |
| WO2011050147A1 (en) | 2009-10-21 | 2011-04-28 | Bionanomatrix, Inc . | Methods and related devices for single molecule whole genome analysis |
| US9447152B2 (en) | 2008-07-07 | 2016-09-20 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| US8784623B2 (en) | 2008-07-17 | 2014-07-22 | Koninklijke Philips N.V. | Nanopore device and a method for nucleic acid analysis |
| EP3029467B1 (en) | 2008-09-22 | 2020-01-08 | University of Washington | Msp nanopores and related methods |
| CN201302544Y (zh) | 2008-12-04 | 2009-09-02 | 浙江大学 | 高俘获率高灵敏度的微流控spr生物传感装置 |
| US8394584B2 (en) | 2008-12-19 | 2013-03-12 | The Board Of Trustees Of The University Of Illinois | Detecting and sorting methylated DNA using a synthetic nanopore |
| CN101519695B (zh) * | 2009-02-19 | 2012-04-18 | 中国人民解放军第三军医大学第一附属医院 | 多靶标量子点标记核酸芯片及其制备方法和检测方法 |
| WO2010111602A1 (en) | 2009-03-26 | 2010-09-30 | Trustees Of Boston University | Method for imaging on thin solid-state interface between two fluids |
| JP5372570B2 (ja) | 2009-03-30 | 2013-12-18 | 株式会社日立ハイテクノロジーズ | ナノポアを用いたバイオポリマー決定方法、システム、及びキット |
| US8986928B2 (en) | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| WO2010129869A1 (en) | 2009-05-07 | 2010-11-11 | The Trustees Of Boston University | Manufacture of nanoparticles using nanopores and voltage-driven electrolyte flow |
| US8926904B2 (en) | 2009-05-12 | 2015-01-06 | Daniel Wai-Cheong So | Method and apparatus for the analysis and identification of molecules |
| US9255002B2 (en) | 2009-09-22 | 2016-02-09 | Cornell University | Apparatus and method for forming self-assembly arrays |
| JP2013506418A (ja) * | 2009-09-30 | 2013-02-28 | クアンタポール, インコーポレイテッド | 標識されたナノポアを使用する生物学的なポリマーの超高速配列決定 |
| US20140335513A9 (en) | 2009-09-30 | 2014-11-13 | Quantapore, Inc. | Hybrid nanopore device with optical detection and methods of using same |
| BR112012013074B1 (pt) | 2009-12-01 | 2018-09-18 | Oxford Nanopore Technologies Limited | instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica |
| CA2795042A1 (en) | 2010-03-30 | 2011-10-13 | Trustees Of Boston University | Tools and method for nanopores unzipping-dependent nucleic acid sequencing |
| WO2011143340A2 (en) | 2010-05-11 | 2011-11-17 | Trustees Of Boston University | Use of nanopore arrays for multiplex sequencing of nucleic acids |
| US8865078B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| CN103069267B (zh) | 2010-09-29 | 2016-05-11 | 株式会社日立高新技术 | 生物聚合物的光学的解析装置以及方法 |
| WO2012121756A1 (en) * | 2011-03-04 | 2012-09-13 | Quantapore, Inc. | Apparatus and methods for performing optical nanopore detection or sequencing |
| US8894796B2 (en) | 2011-06-06 | 2014-11-25 | The University Of North Carolina At Greensboro | Nanopore fabrication and applications thereof |
| US20130040827A1 (en) | 2011-08-14 | 2013-02-14 | Stephen C. Macevicz | Method and compositions for detecting and sequencing nucleic acids |
| GB201119032D0 (en) | 2011-11-03 | 2011-12-14 | Isis Innovation | Multisomes: encapsulated droplet networks |
| GB2497784A (en) | 2011-12-20 | 2013-06-26 | Base4 Innovation Ltd | Identification of polymers using nanopore devices |
| GB201212135D0 (en) | 2012-07-09 | 2012-08-22 | Base4 Innovation Ltd | Improved sequencing apparatus |
| WO2014066905A2 (en) * | 2012-10-28 | 2014-05-01 | Quantapore, Inc. | Reducing background fluorescence in mems materials by low energy ion beam treatment |
| AU2014268322B2 (en) * | 2013-05-24 | 2019-01-24 | Quantapore, Inc. | Nanopore-based nucleic acid analysis with mixed FRET detection |
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| CN107109472B (zh) | 2021-05-11 |
| US20160122812A1 (en) | 2016-05-05 |
| CN107109472A (zh) | 2017-08-29 |
| CA2963604C (en) | 2023-02-14 |
| JP2017531431A (ja) | 2017-10-26 |
| AU2015330841B2 (en) | 2019-08-15 |
| JP2018157835A (ja) | 2018-10-11 |
| JP6740222B2 (ja) | 2020-08-12 |
| EP3204519A1 (en) | 2017-08-16 |
| US20200354787A1 (en) | 2020-11-12 |
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