JP6656210B2 - 分光蛍光光度計 - Google Patents

分光蛍光光度計 Download PDF

Info

Publication number
JP6656210B2
JP6656210B2 JP2017141929A JP2017141929A JP6656210B2 JP 6656210 B2 JP6656210 B2 JP 6656210B2 JP 2017141929 A JP2017141929 A JP 2017141929A JP 2017141929 A JP2017141929 A JP 2017141929A JP 6656210 B2 JP6656210 B2 JP 6656210B2
Authority
JP
Japan
Prior art keywords
sample
fluorescence
spectrum
wavelength
excitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017141929A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019020362A (ja
JP2019020362A5 (enExample
Inventor
純 堀込
純 堀込
莉乃 中島
莉乃 中島
佐藤　洋一
洋一 佐藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi High Tech Science Corp
Original Assignee
Hitachi High Tech Science Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Tech Science Corp filed Critical Hitachi High Tech Science Corp
Priority to JP2017141929A priority Critical patent/JP6656210B2/ja
Priority to EP18184562.9A priority patent/EP3431942B1/en
Priority to US16/041,661 priority patent/US10551315B2/en
Publication of JP2019020362A publication Critical patent/JP2019020362A/ja
Publication of JP2019020362A5 publication Critical patent/JP2019020362A5/ja
Application granted granted Critical
Publication of JP6656210B2 publication Critical patent/JP6656210B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0254—Spectrometers, other than colorimeters, making use of an integrating sphere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/065—Integrating spheres

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
JP2017141929A 2017-07-21 2017-07-21 分光蛍光光度計 Active JP6656210B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2017141929A JP6656210B2 (ja) 2017-07-21 2017-07-21 分光蛍光光度計
EP18184562.9A EP3431942B1 (en) 2017-07-21 2018-07-19 Fluorescence spectrophotometer and fluorescence spectrometry and imaging method
US16/041,661 US10551315B2 (en) 2017-07-21 2018-07-20 Fluorescence spectrophotometer and fluorescence spectrometry and imaging method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2017141929A JP6656210B2 (ja) 2017-07-21 2017-07-21 分光蛍光光度計

Publications (3)

Publication Number Publication Date
JP2019020362A JP2019020362A (ja) 2019-02-07
JP2019020362A5 JP2019020362A5 (enExample) 2019-08-08
JP6656210B2 true JP6656210B2 (ja) 2020-03-04

Family

ID=63012945

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017141929A Active JP6656210B2 (ja) 2017-07-21 2017-07-21 分光蛍光光度計

Country Status (3)

Country Link
US (1) US10551315B2 (enExample)
EP (1) EP3431942B1 (enExample)
JP (1) JP6656210B2 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7284457B2 (ja) 2019-09-02 2023-05-31 株式会社日立ハイテクサイエンス 量子効率分布の取得方法、量子効率分布の表示方法、量子効率分布の取得プログラム、量子効率分布の表示プログラム、分光蛍光光度計及び表示装置
CN110426380B (zh) * 2019-09-29 2020-01-21 常州星宇车灯股份有限公司 一种透射式可控温的激光激发远程荧光材料的测试装置
JP7247844B2 (ja) * 2019-10-01 2023-03-29 株式会社島津製作所 光学分析装置及び方法
CN112665826A (zh) * 2019-10-15 2021-04-16 成都辰显光电有限公司 积分球检测器
CN114467018B (zh) * 2019-11-06 2025-08-26 乌斯特技术股份公司 用于光学表征纺织品样品的设备和方法
JP7191901B2 (ja) * 2020-07-27 2022-12-19 日本電子株式会社 試料分析装置及び方法
JP7626616B2 (ja) * 2020-12-25 2025-02-04 株式会社日立ハイテクサイエンス 分光蛍光光度計、分光蛍光測定方法及び画像撮像方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2799191B2 (ja) * 1989-08-24 1998-09-17 オリンパス光学工業株式会社 細胞内イオンの２次元濃度分布像を形成する方法
GB2241080B (en) * 1990-02-19 1994-06-01 Perkin Elmer Ltd Improvements in or relating to analytical-sampling devices and associated spectrophotometric apparatus and method
JP3577164B2 (ja) * 1996-05-22 2004-10-13 オリンパス株式会社 照明装置及び色・形状測定装置
JPH11118603A (ja) * 1997-10-15 1999-04-30 Minolta Co Ltd 蛍光試料の分光特性測定装置及びその測定方法
US6826424B1 (en) * 2000-12-19 2004-11-30 Haishan Zeng Methods and apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices
US6583879B1 (en) * 2002-01-11 2003-06-24 X-Rite, Incorporated Benchtop spectrophotometer with improved targeting
JP2003315153A (ja) * 2002-04-23 2003-11-06 Minolta Co Ltd 測色装置
EP1700469A4 (en) * 2003-12-19 2008-01-16 Datacolor Holding Ag DIGITAL CAMERA SPECTROPHOTOMETER
JP2005204958A (ja) * 2004-01-23 2005-08-04 Pentax Corp 自家蛍光観察可能な電子内視鏡装置およびシステム
JP4459710B2 (ja) * 2004-05-11 2010-04-28 Ｈｏｙａ株式会社 蛍光観察内視鏡装置
FR2873815B1 (fr) * 2004-08-02 2006-11-24 Inodiag Sa Dispositif de lecture pour lames portant des micro depots supports de reaction biologique
JP3132835U (ja) * 2007-04-09 2007-06-21 株式会社島津製作所 ３次元測定装置
JP2009008509A (ja) * 2007-06-27 2009-01-15 Shinshu Univ 発光量子効率測定装置
JP5161755B2 (ja) * 2008-12-25 2013-03-13 浜松ホトニクス株式会社 分光測定装置、分光測定方法、及び分光測定プログラム
EP2545846B1 (en) * 2010-03-09 2015-05-06 Shiseido Company, Ltd. Lighting device, image analysis device, image analysis method, and evaluation method
JP5867073B2 (ja) 2011-12-28 2016-02-24 富士通株式会社 樹脂硬化状態モニタリング装置及び樹脂硬化状態モニタリング方法
WO2013190618A1 (ja) * 2012-06-18 2013-12-27 株式会社島津製作所 分光蛍光光度計
JP6287883B2 (ja) * 2015-02-16 2018-03-07 株式会社島津製作所 分光蛍光光度計及びそれに用いられる補正関数作成方法
WO2017056830A1 (ja) 2015-10-02 2017-04-06 シャープ株式会社 蛍光検出装置

Also Published As

Publication number Publication date
JP2019020362A (ja) 2019-02-07
US10551315B2 (en) 2020-02-04
EP3431942A1 (en) 2019-01-23
EP3431942B1 (en) 2024-03-06
US20190025208A1 (en) 2019-01-24

Similar Documents

Publication Publication Date Title
JP6656210B2 (ja) 分光蛍光光度計
US20080272312A1 (en) Hyperspectral fluorescence and absorption bioimaging
JP6919890B2 (ja) 光分析装置用の表示装置
JP5507247B2 (ja) 画像駆動分析による分光器顕微鏡法
US20200064270A1 (en) Raman Spectroscopy for Minerals Identification
CN112166314A (zh) 荧光观察设备和荧光观察方法
JP2012032183A (ja) 試料観測装置および試料観測方法
US10663345B2 (en) Raman spectroscopy for minerals identification
JP2021096359A (ja) 蛍光観察機能を備えるラマン顕微装置
CN102680450A (zh) 测定装置及测定方法
JP2010151801A (ja) ラマンイメージング装置
US11486828B2 (en) Fluorescence photometer and observation method
JP7284457B2 (ja) 量子効率分布の取得方法、量子効率分布の表示方法、量子効率分布の取得プログラム、量子効率分布の表示プログラム、分光蛍光光度計及び表示装置
JP2013003386A (ja) 撮像装置およびバーチャルスライド装置
JPH08145888A (ja) 蛍光測色装置
JP7112994B2 (ja) 蛍光撮影装置
JP5204672B2 (ja) Ｘ線分光情報取得方法及びｘ線分光装置
JP2009002795A (ja) 蛍光ｘ線分析装置
CN112585450B (zh) 光谱成像装置和荧光观察装置
JP7626616B2 (ja) 分光蛍光光度計、分光蛍光測定方法及び画像撮像方法
US12399107B1 (en) Integrated spectroscopic system with cuvette holders and homogenized illumination
WO2021034456A2 (en) Raman spectroscopy for minerals identification
Mandelis Focus on photonics, spectroscopy, and spectrometry
JPH10227694A (ja) 発光現象識別装置
JP2024090256A (ja) 光学識別装置および方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190627

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190627

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20190627

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20190709

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190729

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190910

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191105

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200114

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200204

R150 Certificate of patent or registration of utility model

Ref document number: 6656210

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350