JP6563482B2 - 修飾ヌクレオチドリンカー - Google Patents

修飾ヌクレオチドリンカー Download PDF

Info

Publication number
JP6563482B2
JP6563482B2 JP2017506886A JP2017506886A JP6563482B2 JP 6563482 B2 JP6563482 B2 JP 6563482B2 JP 2017506886 A JP2017506886 A JP 2017506886A JP 2017506886 A JP2017506886 A JP 2017506886A JP 6563482 B2 JP6563482 B2 JP 6563482B2
Authority
JP
Japan
Prior art keywords
nucleotide
nucleoside
formula
linker
alkyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017506886A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017525695A5 (enExample
JP2017525695A (ja
Inventor
ウー シャオリン
ウー シャオリン
リュー シャオハイ
リュー シャオハイ
Original Assignee
イルミナ ケンブリッジ リミテッド
イルミナ ケンブリッジ リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イルミナ ケンブリッジ リミテッド, イルミナ ケンブリッジ リミテッド filed Critical イルミナ ケンブリッジ リミテッド
Publication of JP2017525695A publication Critical patent/JP2017525695A/ja
Publication of JP2017525695A5 publication Critical patent/JP2017525695A5/ja
Application granted granted Critical
Publication of JP6563482B2 publication Critical patent/JP6563482B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14—Pyrrolo-pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2017506886A 2014-08-08 2015-08-06 修飾ヌクレオチドリンカー Active JP6563482B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1414098.2 2014-08-08
GBGB1414098.2A GB201414098D0 (en) 2014-08-08 2014-08-08 Modified nucleotide linkers
PCT/GB2015/052282 WO2016020691A1 (en) 2014-08-08 2015-08-06 Modified nucleotide linkers

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019136261A Division JP2020015722A (ja) 2014-08-08 2019-07-24 修飾ヌクレオチドリンカー

Publications (3)

Publication Number Publication Date
JP2017525695A JP2017525695A (ja) 2017-09-07
JP2017525695A5 JP2017525695A5 (enExample) 2018-06-28
JP6563482B2 true JP6563482B2 (ja) 2019-08-21

Family

ID=51629525

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017506886A Active JP6563482B2 (ja) 2014-08-08 2015-08-06 修飾ヌクレオチドリンカー
JP2019136261A Pending JP2020015722A (ja) 2014-08-08 2019-07-24 修飾ヌクレオチドリンカー
JP2021148730A Active JP7726707B2 (ja) 2014-08-08 2021-09-13 修飾ヌクレオチドリンカー

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2019136261A Pending JP2020015722A (ja) 2014-08-08 2019-07-24 修飾ヌクレオチドリンカー
JP2021148730A Active JP7726707B2 (ja) 2014-08-08 2021-09-13 修飾ヌクレオチドリンカー

Country Status (19)

Country Link
US (3) US10190157B2 (enExample)
EP (2) EP3177634B1 (enExample)
JP (3) JP6563482B2 (enExample)
KR (2) KR102287718B1 (enExample)
CN (2) CN106604926B (enExample)
AU (2) AU2015298469B2 (enExample)
BR (2) BR112017002251B1 (enExample)
CA (1) CA2957382C (enExample)
DK (2) DK3564252T3 (enExample)
ES (2) ES2875557T3 (enExample)
GB (1) GB201414098D0 (enExample)
IL (1) IL250498B (enExample)
MX (2) MX385213B (enExample)
MY (1) MY195167A (enExample)
NZ (1) NZ728810A (enExample)
RU (1) RU2699993C2 (enExample)
SG (3) SG10202000850XA (enExample)
WO (1) WO2016020691A1 (enExample)
ZA (1) ZA201700838B (enExample)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414098D0 (en) * 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
EP3091026B1 (en) * 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
WO2017058953A1 (en) 2015-09-28 2017-04-06 The Trustees Of Columbia University In The City Of New York Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
EP3463381A4 (en) 2016-05-23 2020-01-01 The Trustees of Columbia University in the City of New York NUCLEOTIDE DERIVATIVES AND METHOD FOR USE THEREOF
EP3558510B1 (en) * 2016-12-22 2022-11-23 Illumina, Inc. Array including sequencing primer and non-sequencing entity
US11591647B2 (en) 2017-03-06 2023-02-28 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
US12018325B2 (en) 2017-03-28 2024-06-25 The Trustees Of Columbia University In The City Of New York 3′-O-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for DNA sequencing by synthesis
CN110650968B (zh) * 2017-10-11 2022-07-05 深圳华大智造科技股份有限公司 修饰的核苷或核苷酸
WO2019164977A1 (en) 2018-02-21 2019-08-29 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
US12188086B2 (en) 2018-03-15 2025-01-07 The Trustees Of Columbia University In The City Of New York Nucleotide analogues and use thereof for nucleic acid sequencing and analysis preliminary class
CN113039191B (zh) 2018-10-25 2024-07-16 奇异基因组学系统公司 核苷酸类似物
EP3894598B1 (en) 2018-12-14 2024-01-03 Illumina Cambridge Limited Decreasing phasing with unlabeled nucleotides during sequencing
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
WO2020146397A1 (en) 2019-01-08 2020-07-16 Singular Genomics Systems, Inc. Nucleotide cleavable linkers and uses thereof
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
WO2020178162A1 (en) 2019-03-01 2020-09-10 Illumina Cambridge Limited Exocyclic amine-substituted coumarin compounds and their uses as fluorescent labels
WO2020178165A1 (en) 2019-03-01 2020-09-10 Illumina Cambridge Limited Tertiary amine substituted coumarin compounds and their uses as fluorescent labels
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
JP2023510454A (ja) 2019-11-27 2023-03-14 イルミナ ケンブリッジ リミテッド シクロオクタテトラエンを含有する色素及び組成物
JP7287299B2 (ja) 2020-01-31 2023-06-06 トヨタ自動車株式会社 車両および車両制御インターフェース
JP7797426B2 (ja) 2020-06-22 2026-01-13 イルミナ ケンブリッジ リミテッド ３’アセタールブロッキング基を有するヌクレオシド及びヌクレオチド
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
WO2022233795A1 (en) 2021-05-05 2022-11-10 Illumina Cambridge Limited Fluorescent dyes containing bis-boron fused heterocycles and uses in sequencing
MX2023012659A (es) 2021-05-20 2023-12-07 Illumina Inc Composiciones y metodos para secuenciación por síntesis.
JP2024520279A (ja) * 2021-05-26 2024-05-24 イルミナ インコーポレイテッド ３’－ブロック化ヌクレオチドの精製及び重合
US20230304086A1 (en) 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
US20230314322A1 (en) 2022-03-29 2023-10-05 Illumina, Inc. Systems and methods of sequencing polynucleotides
CA3223128A1 (en) 2022-03-31 2023-10-05 Illumina, Inc. Compositions and methods for improving sequencing signals
CA3222797A1 (en) 2022-03-31 2023-10-05 Ramesh NEELAKANDAN Nucleosides and nucleotides with 3' vinyl blocking group
AU2023281871A1 (en) 2022-05-31 2024-11-14 Illumina, Inc Compositions and methods for nucleic acid sequencing
WO2024003087A1 (en) 2022-06-28 2024-01-04 Illumina, Inc. Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
EP4594428A2 (en) 2022-09-30 2025-08-06 Illumina, Inc. Compositions and methods for reducing photo damage during sequencing
AU2023409138A1 (en) 2022-12-22 2024-10-03 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
WO2024137774A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
CA3246559A1 (en) 2022-12-27 2024-07-04 Illumina, Inc. SEQUENCE PROCESSES USING ALLYL 3' BLOCKED NUCLEOTIDES
US20240327910A1 (en) 2023-03-29 2024-10-03 Illumina, Inc. Naphthalimide dyes and uses in nucleic acid sequencing
CA3257470A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. NUCLEIC ACID COMPOSITIONS AND SEQUENCE METHODS
WO2025049700A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing
US20250137048A1 (en) 2023-10-26 2025-05-01 Illumina, Inc. 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing
WO2025106715A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Conjugated polymers or polymer dots as fluorescent labels
US20250197933A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Hydrogel nanoparticles as labeling scaffold in sequencing
WO2025144716A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
US20250215494A1 (en) 2023-12-29 2025-07-03 Illumina, Inc. Tricyclic polymethine dyes for nucleic acid sequencing
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025221895A1 (en) 2024-04-19 2025-10-23 Illumina, Inc. Water soluble polymer scaffolds for dye labeling

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
DE3924454A1 (de) 1989-07-24 1991-02-07 Cornelis P Prof Dr Hollenberg Die anwendung von dna und dna-technologie fuer die konstruktion von netzwerken zur verwendung in der chip-konstruktion und chip-produktion (dna chips)
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
DK0604552T3 (da) 1991-09-18 1997-08-04 Affymax Tech Nv Fremgangsmåde til syntese af forskellige samlinger af oligomerer
EP1588761A3 (en) 1991-11-22 2005-11-23 Affymetrix, Inc. Method of forming arrays of polymers
ATE278807T1 (de) 1992-02-19 2004-10-15 New York Health Res Inst Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren
US5583211A (en) 1992-10-29 1996-12-10 Beckman Instruments, Inc. Surface activated organic polymers useful for location - specific attachment of nucleic acids, peptides, proteins and oligosaccharides
US5472672A (en) 1993-10-22 1995-12-05 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for polymer synthesis using arrays
DE69433180T2 (de) 1993-10-26 2004-06-24 Affymetrix, Inc., Santa Clara Felder von nukleinsaeuresonden auf biologischen chips
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5429807A (en) 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5545531A (en) 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
ATE496288T1 (de) 1995-10-11 2011-02-15 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
US5658734A (en) 1995-10-17 1997-08-19 International Business Machines Corporation Process for synthesizing chemical compounds
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
US6087102A (en) 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays
US6287776B1 (en) 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Ｄｎａプローブアレー
US6031078A (en) 1998-06-16 2000-02-29 Millennium Pharmaceuticals, Inc. MTbx protein and nucleic acid molecules and uses therefor
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
ATE319857T1 (de) * 1998-12-14 2006-03-15 Li Cor Inc Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese
US6379698B1 (en) 1999-04-06 2002-04-30 Isis Pharmaceuticals, Inc. Fusogenic lipids and vesicles
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
EP1196630B2 (en) 1999-04-20 2018-10-17 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20050244870A1 (en) 1999-04-20 2005-11-03 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
CA2412567A1 (en) 2000-06-07 2001-12-13 Li-Cor, Inc. Charge-switch nucleotides
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US20040259082A1 (en) * 2001-04-24 2004-12-23 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymers
DE10120798B4 (de) * 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
DE10120797B4 (de) 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
ES2326077T3 (es) 2002-08-23 2009-09-30 Illumina Cambridge Limited Nucleotidos marcados.
EP3795577A1 (en) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modified nucleotides
US7414116B2 (en) * 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
EP1616034A4 (en) * 2003-04-01 2008-12-03 Activx Biosciences Inc ACYL PHOSPHATE AND PHOSPHONATE EASTS AND PROCESSES FOR THEIR SYNTHESIS AND USE IN PROTEAM ANALYSIS
EP1725572B1 (de) * 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US20070117104A1 (en) 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
WO2006044078A2 (en) 2004-09-17 2006-04-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US8212020B2 (en) 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
EP1885876A2 (de) * 2005-03-17 2008-02-13 Genovoxx GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007123744A2 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
USRE49362E1 (en) 2006-05-18 2023-01-10 Illumina Cambridge Limited Dye compounds and the use of their labelled conjugates
EP2073820A4 (en) 2006-09-15 2014-07-16 Belrose Pharma Inc TARGETED POLYMER PRODRUGS WITH MULTIFUNCTIONAL CONNECTORS
KR20090057235A (ko) 2006-09-15 2009-06-04 엔존 파마슈티컬즈, 인코포레이티드 리신계 폴리머 링커
AU2007296189A1 (en) 2006-09-15 2008-03-20 Enzon Pharmaceuticals, Inc. Polyalkylene oxides having hindered ester-based biodegradable linkers
MX2009002859A (es) 2006-09-15 2009-03-30 Enzon Pharmaceuticals Inc Enlazadores biodegradables a base de ester impedido para suministro de oligonucleotidos.
US8110559B2 (en) 2006-09-15 2012-02-07 Enzon Pharmaceuticals, Inc. Hindered ester-based biodegradable linkers for oligonucleotide delivery
WO2008042067A2 (en) * 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
WO2008070749A2 (en) 2006-12-05 2008-06-12 Lasergen, Inc. Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing
EP2639579B1 (en) 2006-12-14 2016-11-16 Life Technologies Corporation Apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7678894B2 (en) * 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
US9163053B2 (en) * 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
WO2009054922A1 (en) 2007-10-19 2009-04-30 The Trustees Of Columbia University In The City Of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP2207900B1 (en) * 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9206216B2 (en) * 2010-04-21 2015-12-08 Pierce Biotechnology, Inc. Modified nucleotides methods and kits
WO2012159072A2 (en) * 2011-05-18 2012-11-22 Cayman Chemical Company, Incorporated Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins
RS62530B1 (sr) * 2011-09-23 2021-11-30 Illumina Inc Sastavi za sekvenciranje nukleinskih kiselina
ES2672880T3 (es) * 2011-11-17 2018-06-18 Indiana University Research And Technology Corporation Péptidos de la superfamilia de glucagón que muestran actividad de receptor de glucocorticoides
WO2013101690A1 (en) * 2011-12-29 2013-07-04 modeRNA Therapeutics Modified mrnas encoding cell-penetrating polypeptides
CN103087131B (zh) * 2013-01-15 2015-07-08 上海交通大学 可逆终端及其合成和在dna合成测序中的用途
EP2964612B1 (en) 2013-03-08 2017-02-01 Illumina Cambridge Limited Polymethine compounds and their use as fluorescent labels
CN105263918B (zh) 2013-03-08 2018-05-18 伊鲁米纳剑桥有限公司 罗丹明化合物及其作为荧光标记物的用途
CN103484106B (zh) 2013-09-05 2015-08-19 上海交通大学 四色荧光标记可逆终端及其在dna测序中的用途
CN103866010B (zh) 2014-02-28 2016-04-20 郭诚 一种基因测序的方法
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers

Also Published As

Publication number Publication date
EP3564252A1 (en) 2019-11-06
US10190157B2 (en) 2019-01-29
CA2957382C (en) 2022-05-31
SG10202000850XA (en) 2020-03-30
GB201414098D0 (en) 2014-09-24
AU2015298469B2 (en) 2018-12-06
MX385213B (es) 2025-03-14
US11773438B2 (en) 2023-10-03
DK3177634T3 (da) 2020-03-02
US20220186306A1 (en) 2022-06-16
CN106604926A (zh) 2017-04-26
SG10201900948PA (en) 2019-03-28
JP7726707B2 (ja) 2025-08-20
WO2016020691A1 (en) 2016-02-11
MX2017001694A (es) 2017-04-27
BR112017002251A2 (pt) 2019-04-09
ES2875557T3 (es) 2021-11-10
CN111704643A (zh) 2020-09-25
AU2015298469A1 (en) 2017-02-23
BR112017002251B1 (pt) 2022-01-25
CN106604926B (zh) 2020-05-15
RU2017103539A3 (enExample) 2018-11-15
MX377448B (es) 2025-03-10
EP3177634B1 (en) 2019-12-18
US20190153529A1 (en) 2019-05-23
DK3564252T3 (da) 2021-05-25
IL250498B (en) 2021-03-25
MY195167A (en) 2023-01-11
RU2017103539A (ru) 2018-09-10
SG11201700880RA (en) 2017-03-30
EP3177634A1 (en) 2017-06-14
KR20170039743A (ko) 2017-04-11
RU2699993C2 (ru) 2019-09-12
ZA201700838B (en) 2019-06-26
KR20210100205A (ko) 2021-08-13
KR102491869B1 (ko) 2023-01-26
US11230734B2 (en) 2022-01-25
CA2957382A1 (en) 2016-02-11
EP3564252B1 (en) 2021-03-17
BR112017002251A8 (pt) 2020-05-12
ES2773438T3 (es) 2020-07-13
AU2018278843A1 (en) 2019-01-03
US20160040225A1 (en) 2016-02-11
JP2022000435A (ja) 2022-01-04
IL250498A0 (en) 2017-03-30
MX2020009401A (es) 2021-08-09
AU2018278843B2 (en) 2019-07-18
NZ748150A (en) 2023-03-31
NZ728810A (en) 2018-11-30
JP2017525695A (ja) 2017-09-07
JP2020015722A (ja) 2020-01-30
BR122020018717B1 (pt) 2022-01-25
KR102287718B1 (ko) 2021-08-09

Similar Documents

Publication Publication Date Title
JP7726707B2 (ja) 修飾ヌクレオチドリンカー
US20230383342A1 (en) Compositions and methods for nucleic acid sequencing
US20110020827A1 (en) Modified nucleosides and nucleotides and uses thereof
US20230304086A1 (en) Labeled avidin and methods for sequencing
US20250197933A1 (en) Hydrogel nanoparticles as labeling scaffold in sequencing
HK40009716B (en) Modified nucleotide linkers
HK40009716A (en) Modified nucleotide linkers
HK1233269B (en) Modified nucleotide linkers
HK1233269A1 (en) Modified nucleotide linkers

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180517

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180517

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190625

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20190724

R150 Certificate of patent or registration of utility model

Ref document number: 6563482

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250