JP6466173B2 - ブロック型プライマーを用いた等温増幅法 - Google Patents

ブロック型プライマーを用いた等温増幅法 Download PDF

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JP6466173B2
JP6466173B2 JP2014557780A JP2014557780A JP6466173B2 JP 6466173 B2 JP6466173 B2 JP 6466173B2 JP 2014557780 A JP2014557780 A JP 2014557780A JP 2014557780 A JP2014557780 A JP 2014557780A JP 6466173 B2 JP6466173 B2 JP 6466173B2
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primer
flap
target
rnase
amplification
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JP2015507931A (ja
JP2015507931A5 (enExample
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ディー． ジェニソン，ロバート
ディー． ジェニソン，ロバート
ヒック，ブライアン
ウェスト，マデリン
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ヴェラ オペレーションズ ユーエスエー，インコーポレーテッド
ヴェラ オペレーションズ ユーエスエー，インコーポレーテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014557780A 2012-02-14 2013-02-14 ブロック型プライマーを用いた等温増幅法 Active JP6466173B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261598648P 2012-02-14 2012-02-14
US61/598,648 2012-02-14
PCT/US2013/026202 WO2013123238A1 (en) 2012-02-14 2013-02-14 Methods of isothermal amplification using blocked primers

Publications (3)

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JP2015507931A JP2015507931A (ja) 2015-03-16
JP2015507931A5 JP2015507931A5 (enExample) 2016-03-31
JP6466173B2 true JP6466173B2 (ja) 2019-02-06

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US (2) US8936921B2 (enExample)
EP (1) EP2814975A4 (enExample)
JP (1) JP6466173B2 (enExample)
CA (1) CA2881200A1 (enExample)
HK (1) HK1205194A1 (enExample)
WO (1) WO2013123238A1 (enExample)

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WO2014143228A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
WO2010111265A1 (en) 2009-03-24 2010-09-30 University Of Chicago Slip chip device and methods
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
WO2013159116A1 (en) 2012-04-20 2013-10-24 University Of Chicago Fluidic devices for biospecimen preservation
WO2013159117A1 (en) 2012-04-20 2013-10-24 SlipChip, LLC Fluidic devices and systems for sample preparation or autonomous analysis
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
US9074248B1 (en) * 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
WO2017136387A1 (en) * 2016-02-01 2017-08-10 Integrated Dna Technologies, Inc. Cleavable primers for isothermal amplification
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
WO2018180987A1 (ja) * 2017-03-29 2018-10-04 東洋紡株式会社 核酸の検出方法
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
CA3079687A1 (en) * 2017-10-18 2019-04-25 Chiahao TSUI Selective enrichment of a population of dna in a mixed dna sample through targeted suppression of dna amplification
EP4310196A3 (en) 2017-11-07 2024-04-24 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
US12226745B2 (en) 2017-11-07 2025-02-18 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
CN109913538A (zh) * 2018-09-06 2019-06-21 湖南融健基因生物科技有限公司 一种快速检测snp位点的荧光定量试剂盒
EP4403648B1 (en) * 2019-05-03 2025-08-20 Life Technologies Corporation Methods and compositions for manipulating nucleic acids

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US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
CN100351393C (zh) 2001-02-15 2007-11-28 宝生物工程株式会社 核苷酸多态性的检测方法
TW200404889A (en) 2002-05-31 2004-04-01 Takara Bio Inc Method of typing gene polymorphisms
US7662594B2 (en) * 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
AU2003272438B2 (en) 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
WO2007120808A2 (en) 2006-04-14 2007-10-25 Biohelix Corporation A method of asymmetric helicase-dependent amplification for probe-based detection of targets
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2644707B1 (en) * 2008-04-30 2015-06-03 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
JP2013507910A (ja) * 2009-10-13 2013-03-07 シンテッツァ モレキュラー ディテクション イスラエル リミテッド 核酸試料から標的配列を増幅するための方法および組成物
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CA2799200A1 (en) * 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

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US20140017730A1 (en) 2014-01-16
CA2881200A1 (en) 2013-08-22
JP2015507931A (ja) 2015-03-16
US20150176068A1 (en) 2015-06-25
US8936921B2 (en) 2015-01-20
WO2013123238A1 (en) 2013-08-22
HK1205194A1 (en) 2015-12-11
EP2814975A4 (en) 2015-12-30
US9896723B2 (en) 2018-02-20
EP2814975A1 (en) 2014-12-24

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