HK1205194A1 - Methods of isothermal amplification using blocked primers - Google Patents
Methods of isothermal amplification using blocked primers Download PDFInfo
- Publication number
- HK1205194A1 HK1205194A1 HK15105687.0A HK15105687A HK1205194A1 HK 1205194 A1 HK1205194 A1 HK 1205194A1 HK 15105687 A HK15105687 A HK 15105687A HK 1205194 A1 HK1205194 A1 HK 1205194A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- methods
- isothermal amplification
- blocked primers
- sequence
- target
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261598648P | 2012-02-14 | 2012-02-14 | |
| US61/598,648 | 2012-02-14 | ||
| PCT/US2013/026202 WO2013123238A1 (en) | 2012-02-14 | 2013-02-14 | Methods of isothermal amplification using blocked primers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HK1205194A1 true HK1205194A1 (en) | 2015-12-11 |
Family
ID=48984714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HK15105687.0A HK1205194A1 (en) | 2012-02-14 | 2013-02-14 | Methods of isothermal amplification using blocked primers |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8936921B2 (enExample) |
| EP (1) | EP2814975A4 (enExample) |
| JP (1) | JP6466173B2 (enExample) |
| CA (1) | CA2881200A1 (enExample) |
| HK (1) | HK1205194A1 (enExample) |
| WO (1) | WO2013123238A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| CN104722342B (zh) | 2009-03-24 | 2017-01-11 | 芝加哥大学 | 滑动式芯片装置和方法 |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| JP6196661B2 (ja) | 2012-04-20 | 2017-09-13 | スリップチップ， エルエルシー | サンプル調製または自律分析のための流体デバイスおよびシステム |
| WO2013159116A1 (en) | 2012-04-20 | 2013-10-24 | University Of Chicago | Fluidic devices for biospecimen preservation |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| US9074248B1 (en) * | 2012-12-18 | 2015-07-07 | Qiagen Gmbh | Primers for helicase dependent amplification and their methods of use |
| AU2013381709A1 (en) * | 2013-03-15 | 2015-10-01 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| WO2017136387A1 (en) * | 2016-02-01 | 2017-08-10 | Integrated Dna Technologies, Inc. | Cleavable primers for isothermal amplification |
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| JP7279633B2 (ja) * | 2017-03-29 | 2023-05-23 | 東洋紡株式会社 | 核酸の検出方法 |
| ES3029737T3 (en) | 2017-10-06 | 2025-06-25 | 10X Genomics Inc | Rna templated ligation |
| JP2021500077A (ja) | 2017-10-18 | 2021-01-07 | デイ ゼロ ダイアグノスティックス， インコーポレイテッド | Ｄｎａ増幅の標的化抑制による混合ｄｎａ試料中のｄｎａ集団の選択的富化 |
| EP3707274B1 (en) | 2017-11-07 | 2023-09-13 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| US12226745B2 (en) | 2017-11-07 | 2025-02-18 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| CN109913538A (zh) * | 2018-09-06 | 2019-06-21 | 湖南融健基因生物科技有限公司 | 一种快速检测snp位点的荧光定量试剂盒 |
| WO2020227031A1 (en) * | 2019-05-03 | 2020-11-12 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
| US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| JP3681729B2 (ja) | 2001-02-15 | 2005-08-10 | タカラバイオ株式会社 | 塩基置換の検出方法 |
| TW200404889A (en) | 2002-05-31 | 2004-04-01 | Takara Bio Inc | Method of typing gene polymorphisms |
| US7662594B2 (en) * | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| US7824890B2 (en) * | 2005-02-19 | 2010-11-02 | Avacta Group Plc | Isothermal amplification of nucleic acids |
| WO2007120808A2 (en) | 2006-04-14 | 2007-10-25 | Biohelix Corporation | A method of asymmetric helicase-dependent amplification for probe-based detection of targets |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| WO2009135093A2 (en) * | 2008-04-30 | 2009-11-05 | Integrated Dna Technologies, Inc. | Rnase-h-based assays utilizing modified rna monomers |
| AU2010307991A1 (en) * | 2009-10-13 | 2012-05-10 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
| CA2786473A1 (en) * | 2010-01-08 | 2011-07-14 | Qiagen Gaithersburg, Inc. | Materials and methods for isothermal nucleic acid amplification |
| AU2011255638B2 (en) * | 2010-05-19 | 2016-08-25 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
-
2013
- 2013-02-14 CA CA2881200A patent/CA2881200A1/en not_active Abandoned
- 2013-02-14 HK HK15105687.0A patent/HK1205194A1/xx unknown
- 2013-02-14 JP JP2014557780A patent/JP6466173B2/ja active Active
- 2013-02-14 WO PCT/US2013/026202 patent/WO2013123238A1/en not_active Ceased
- 2013-02-14 EP EP13748794.8A patent/EP2814975A4/en not_active Withdrawn
- 2013-06-03 US US13/908,827 patent/US8936921B2/en active Active
-
2014
- 2014-12-10 US US14/565,696 patent/US9896723B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US8936921B2 (en) | 2015-01-20 |
| US20150176068A1 (en) | 2015-06-25 |
| EP2814975A4 (en) | 2015-12-30 |
| CA2881200A1 (en) | 2013-08-22 |
| JP2015507931A (ja) | 2015-03-16 |
| EP2814975A1 (en) | 2014-12-24 |
| US9896723B2 (en) | 2018-02-20 |
| WO2013123238A1 (en) | 2013-08-22 |
| JP6466173B2 (ja) | 2019-02-06 |
| US20140017730A1 (en) | 2014-01-16 |
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