JP6430253B2 - 相互作用を検出および測定するための核酸ベースのリンカー - Google Patents

相互作用を検出および測定するための核酸ベースのリンカー Download PDF

Info

Publication number
JP6430253B2
JP6430253B2 JP2014540177A JP2014540177A JP6430253B2 JP 6430253 B2 JP6430253 B2 JP 6430253B2 JP 2014540177 A JP2014540177 A JP 2014540177A JP 2014540177 A JP2014540177 A JP 2014540177A JP 6430253 B2 JP6430253 B2 JP 6430253B2
Authority
JP
Japan
Prior art keywords
nucleic acid
binding
binding partner
oligonucleotide
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014540177A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014532430A (ja
JP2014532430A5 (cg-RX-API-DMAC7.html
Inventor
フィリップ ウォン，ウェズリー
フィリップ ウォン，ウェズリー
アンダース ハルヴォルセン，ケネス
アンダース ハルヴォルセン，ケネス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston Childrens Hospital
Harvard University
Original Assignee
Boston Childrens Hospital
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston Childrens Hospital, Harvard University filed Critical Boston Childrens Hospital
Publication of JP2014532430A publication Critical patent/JP2014532430A/ja
Publication of JP2014532430A5 publication Critical patent/JP2014532430A5/ja
Application granted granted Critical
Publication of JP6430253B2 publication Critical patent/JP6430253B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2014540177A 2011-11-05 2012-11-05 相互作用を検出および測定するための核酸ベースのリンカー Active JP6430253B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161556194P 2011-11-05 2011-11-05
US61/556,194 2011-11-05
US201261666655P 2012-06-29 2012-06-29
US61/666,655 2012-06-29
PCT/US2012/063538 WO2013067489A1 (en) 2011-11-05 2012-11-05 Nucleic acid-based linkers for detecting and measuring interactions

Publications (3)

Publication Number Publication Date
JP2014532430A JP2014532430A (ja) 2014-12-08
JP2014532430A5 JP2014532430A5 (cg-RX-API-DMAC7.html) 2015-12-24
JP6430253B2 true JP6430253B2 (ja) 2018-11-28

Family

ID=48192891

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014540177A Active JP6430253B2 (ja) 2011-11-05 2012-11-05 相互作用を検出および測定するための核酸ベースのリンカー

Country Status (3)

Country Link
US (2) US9914958B2 (cg-RX-API-DMAC7.html)
JP (1) JP6430253B2 (cg-RX-API-DMAC7.html)
WO (1) WO2013067489A1 (cg-RX-API-DMAC7.html)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6430253B2 (ja) * 2011-11-05 2018-11-28 プレジデント アンド フェローズ オブ ハーバード カレッジ 相互作用を検出および測定するための核酸ベースのリンカー
CA2917435A1 (en) 2013-07-10 2015-01-15 Children's Medical Center Corporation Compositions and methods relating to nucleic acid-protein complexes
WO2015164602A2 (en) 2014-04-23 2015-10-29 Children's Medical Center Corporation High-throughput structure determination using nucleic acid calipers
WO2016089588A1 (en) * 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
WO2016196824A1 (en) * 2015-06-02 2016-12-08 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
WO2017003950A2 (en) * 2015-06-27 2017-01-05 Children's Medical Center Corporation Compositions and methods for analyte detection using nanoswitches
WO2017055463A1 (en) * 2015-10-01 2017-04-06 Dynamic Biosensors Gmbh Method for detecting molecular interactions using an immobilized nucleic acid
WO2017139409A1 (en) 2016-02-09 2017-08-17 Children's Medical Center Corporation Method for detection of analytes via polymer complexes
JP2019512722A (ja) 2016-02-25 2019-05-16 ザ チルドレンズ メディカル センター コーポレーション 分子に関連した特徴の測定のためのスピン装置
EP3967651A1 (en) * 2016-03-11 2022-03-16 Children's Medical Center Corporation Nucleic acid nanoswitch catenanes
US10919037B2 (en) 2016-03-23 2021-02-16 Children's Medical Center Corporation Systems and apparatus for detecting compounds in human biological samples
WO2017165647A1 (en) * 2016-03-23 2017-09-28 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
WO2017189794A1 (en) * 2016-04-27 2017-11-02 President And Fellows Of Harvard College Method of secure communication via nucleotide polymers
US10823699B2 (en) 2016-07-29 2020-11-03 Vital Biosciences, Inc. Gel electrophoresis diagnostic kit and methods of using the same
CN115948505B (zh) 2016-08-02 2025-07-25 哈佛学院院长及董事 交叉协同自组装体
US12252507B2 (en) 2016-12-05 2025-03-18 Children's Medical Center Corporation Nucleic acid nanoswitch construction methods
WO2018119437A2 (en) 2016-12-23 2018-06-28 Confer Health, Inc. Electrophoresis diagnostic methods and kits
WO2019100080A1 (en) 2017-11-20 2019-05-23 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
FR3075820B1 (fr) 2017-12-21 2022-12-30 Paris Sciences Lettres Quartier Latin Molecule d'adn double-brin pour la detection et la caracterisation des interactions moleculaires
US11561221B2 (en) * 2018-05-02 2023-01-24 Trustees Of Boston University Dynamic tracking of captured targets for enhanced digital biosensing
KR102767028B1 (ko) 2018-06-06 2025-02-13 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 핵산 라이브러리의 생성 방법 및 이를 실행하기 위한 조성물 및 키트
WO2020163397A2 (en) * 2019-02-04 2020-08-13 Akoya Biosciences, Inc. Analyte detection by selective labeling of biological samples
CN111549098B (zh) * 2020-06-16 2024-10-29 南开大学 测量人端粒精准长度的单分子力学方法
CN111721504B (zh) * 2020-06-17 2022-02-18 中国科学院物理研究所 在小力下测算磁镊高度转换系数的方法及应用
EP4504968A1 (en) 2022-04-01 2025-02-12 Depixus SAS Methods of screening compounds
WO2025041065A1 (en) * 2023-08-21 2025-02-27 Depixus SAS Device for determining molecular interactions

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9114525D0 (en) 1991-07-05 1991-08-21 Delnatte Sabine Homogeneous nucleic acids probe based tests and substances
JPH09500378A (ja) 1993-07-02 1997-01-14 リンクス セラピューティクス，インコーポレイティド 枝分れされそして複雑に結合された高分子構造体の集中的合成
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US5902724A (en) * 1996-07-12 1999-05-11 Tm Technologies, Inc. Signal amplification method
CA2311501A1 (en) 1997-11-25 1999-06-03 Mosaic Technologies Devices and methods for detecting target molecules in biological samples
US20020177144A1 (en) 1997-12-30 2002-11-28 Jose Remacle Detection and/or quantification method of a target molecule by a binding with a capture molecule fixed on the surface of a disc
US6143504A (en) * 1998-10-27 2000-11-07 Arch Development Corporation Methods and compositions for the diagnosis of fragile X syndrome
GB9827912D0 (en) * 1998-12-19 1999-02-10 Univ Manchester Detecting nucleic acids
JP3080951B1 (ja) * 1999-03-04 2000-08-28 株式会社分子バイオホトニクス研究所 ウイルスを検出する方法
EP1404878A4 (en) 2001-07-11 2006-12-06 Applera Corp INTERNAL CALIBRATION STANDARDS FOR ELECTROPHORETIC ANALYZES
US20030143549A1 (en) * 2002-01-29 2003-07-31 Eastman Kodak Company Method for DNA sequencing and gene identification
US7294513B2 (en) 2002-07-24 2007-11-13 Wyatt Technology Corporation Method and apparatus for characterizing solutions of small particles
EP1540013B1 (en) * 2002-08-19 2015-07-08 The President and Fellows of Harvard College Evolving new molecular function
AU2002952797A0 (en) 2002-11-20 2002-12-05 Bio-Molecular Holdings Pty Limited Centrifugal device and method using same
CN103397082B (zh) * 2003-02-26 2017-05-31 考利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
JP4318721B2 (ja) 2004-10-15 2009-08-26 独立行政法人科学技術振興機構 ｍＲＮＡ−ピューロマイシン−タンパク質連結体作製用リンカー
US20070026423A1 (en) * 2005-03-16 2007-02-01 Thomas Koehler Method and test kit for the detection of target nucleic acids
CA2610027A1 (en) * 2005-05-26 2006-11-30 Ensemble Discovery Corporation Biodetection by nucleic acid-templated chemistry
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
WO2007056250A2 (en) 2005-11-04 2007-05-18 U.S. Genomics, Inc. Heterogeneous assay of analytes in solution using polymers
EP1963531B1 (en) 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2008054834A2 (en) * 2006-02-03 2008-05-08 Columbia University Binary deoxyribozyme probes for nucleic acid analysis
WO2007139849A2 (en) * 2006-05-25 2007-12-06 The Arizona Board Of Regents, A Body Corporate Acting On Behalf Of Arizona State University Modified nucleic acid nanoarrays and uses therefor
US20090286694A1 (en) 2006-08-21 2009-11-19 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
JP2008259453A (ja) 2007-04-12 2008-10-30 Toyobo Co Ltd 核酸の検出方法
US8043810B2 (en) 2007-05-02 2011-10-25 Eagle Eye Research, Inc. Analyte detection using autocatalytic chain reactions
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
EP3622968A1 (en) 2008-10-31 2020-03-18 The General Hospital Corporation Compositions and methods for delivering a substance to a biological target
US9354189B2 (en) 2008-12-02 2016-05-31 President And Fellows Of Harvard College Apparatus for measurement of spinning forces relating to molecules
US8491454B2 (en) 2008-12-02 2013-07-23 President And Fellows Of Harvard College Spinning force apparatus
SG177395A1 (en) 2009-07-07 2012-02-28 Agency Science Tech & Res Methods of identifying a pair of binding partners
CA2775613C (en) * 2009-10-13 2018-06-12 Nanostring Technologies, Inc. Protein detection via nanoreporters
WO2011072133A1 (en) 2009-12-09 2011-06-16 William Marsh Rice University Therapeutic compositions and methods for delivery of active agents cleavably linked to nanoparticles
US20130196341A1 (en) 2010-07-06 2013-08-01 T2 Biosystems ,Inc. Methods and compositions for detection of analytes
JP6430253B2 (ja) * 2011-11-05 2018-11-28 プレジデント アンド フェローズ オブ ハーバード カレッジ 相互作用を検出および測定するための核酸ベースのリンカー
US9182334B2 (en) 2012-02-24 2015-11-10 Agilent Technologies, Inc. Method of determining thermodynamic and kinetic parameters from measured off rates
WO2013177046A1 (en) 2012-05-21 2013-11-28 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
WO2014011800A1 (en) 2012-07-10 2014-01-16 Pivot Bio, Inc. Methods for multipart, modular and scarless assembly of dna molecules
CA2917435A1 (en) 2013-07-10 2015-01-15 Children's Medical Center Corporation Compositions and methods relating to nucleic acid-protein complexes
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
US9322051B2 (en) 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
US20150361422A1 (en) 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
WO2016089588A1 (en) 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
WO2017003950A2 (en) 2015-06-27 2017-01-05 Children's Medical Center Corporation Compositions and methods for analyte detection using nanoswitches

Also Published As

Publication number Publication date
US9914958B2 (en) 2018-03-13
JP2014532430A (ja) 2014-12-08
WO2013067489A9 (en) 2013-09-12
US20180291434A1 (en) 2018-10-11
WO2013067489A1 (en) 2013-05-10
US20140255939A1 (en) 2014-09-11

Similar Documents

Publication Publication Date Title
JP6430253B2 (ja) 相互作用を検出および測定するための核酸ベースのリンカー
US20220348990A1 (en) Molecular programming tools
US11198900B2 (en) Nucleic acid-based linkers for detecting and measuring interactions
CN108588208B (zh) 通过单分子操作来检测dna修饰和蛋白结合的方法
KR101924665B1 (ko) 회전-의존성 전사 서열분석 시스템 및 사용 방법
JP6325027B2 (ja) ハイブリダイゼーションによるｄｎａ配列決定法
Halvorsen et al. Nanoengineering a single-molecule mechanical switch using DNA self-assembly
JP6325028B2 (ja) 重合によるｄｎａ配列決定法
CN111630179B (zh) 邻近检测方法和组合物
CN103703145A (zh) 解折叠邻近探针及其使用方法
JP2016507249A (ja) ナノ構造ベースの核酸配列決定のための方法および組成物
WO2017139409A1 (en) Method for detection of analytes via polymer complexes
Bearden et al. Simple and efficient room-temperature release of biotinylated nucleic acids from streptavidin and its application to selective molecular detection
US20250129361A1 (en) Method for massively-parallel screening of aptamer switches
Schmidt et al. Supramolecular Interactions Modulate RNA: DNA Folding Observed via Nanopore Sensing
JPWO2009123255A1 (ja) アプタマー選抜方法及び装置、ならびにアプタマーを用いたセンサ方法
Mandal Development of Single-Molecule Mechanochemical Biosensors for Ultrasensitive and Multiplex Sensing of Analytes

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151105

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151105

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20161202

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170301

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170428

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170601

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20171113

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180213

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180510

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180926

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20181026

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20181031

R150 Certificate of patent or registration of utility model

Ref document number: 6430253

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250