JP6335793B2 - 発現方法 - Google Patents

発現方法 Download PDF

Info

Publication number
JP6335793B2
JP6335793B2 JP2014553753A JP2014553753A JP6335793B2 JP 6335793 B2 JP6335793 B2 JP 6335793B2 JP 2014553753 A JP2014553753 A JP 2014553753A JP 2014553753 A JP2014553753 A JP 2014553753A JP 6335793 B2 JP6335793 B2 JP 6335793B2
Authority
JP
Japan
Prior art keywords
microorganism
nucleic acid
acid sequence
protein
amylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2014553753A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015505463A (ja
Inventor
キュッパース トビアス
キュッパース トビアス
シュテフェン ヴィクトリア
シュテフェン ヴィクトリア
シャルロ アイヒシュテット ルネ
シャルロ アイヒシュテット ルネ
エーファース シュテファン
エーファース シュテファン
マウラー カール−ハインツ
マウラー カール−ハインツ
ボンゲアツ ヨハネス
ボンゲアツ ヨハネス
ヘルムート ヘンドリク
ヘルムート ヘンドリク
ヴェーバー トーマス
ヴェーバー トーマス
オコンネル ティモシー
オコンネル ティモシー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47683703&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP6335793(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by BASF SE filed Critical BASF SE
Publication of JP2015505463A publication Critical patent/JP2015505463A/ja
Application granted granted Critical
Publication of JP6335793B2 publication Critical patent/JP6335793B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2014553753A 2012-01-31 2013-01-29 発現方法 Expired - Fee Related JP6335793B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102012201297A DE102012201297A1 (de) 2012-01-31 2012-01-31 Expressionsverfahren
DE102012201297.4 2012-01-31
PCT/EP2013/051656 WO2013113689A1 (de) 2012-01-31 2013-01-29 Expressionsverfahren

Publications (2)

Publication Number Publication Date
JP2015505463A JP2015505463A (ja) 2015-02-23
JP6335793B2 true JP6335793B2 (ja) 2018-05-30

Family

ID=47683703

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014553753A Expired - Fee Related JP6335793B2 (ja) 2012-01-31 2013-01-29 発現方法

Country Status (15)

Country Link
US (1) US9725704B2 (enExample)
EP (1) EP2809816B9 (enExample)
JP (1) JP6335793B2 (enExample)
KR (1) KR20150000876A (enExample)
CN (1) CN104471066B (enExample)
BR (1) BR112014018534A8 (enExample)
CA (1) CA2862731A1 (enExample)
DE (1) DE102012201297A1 (enExample)
DK (1) DK2809816T3 (enExample)
ES (1) ES2703753T3 (enExample)
IN (1) IN2014DN07148A (enExample)
MX (1) MX351354B (enExample)
PL (1) PL2809816T3 (enExample)
RU (1) RU2644199C2 (enExample)
WO (1) WO2013113689A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (de) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
DE102012215107A1 (de) 2012-08-24 2014-02-27 Basf Se Festes Geschirrspülmittel mit verbesserter Proteaseleistung
CN105483100B (zh) * 2016-01-13 2019-08-13 福建农林大学 一种诱导海洋微生物发酵产κ-卡拉胶酶的组合诱导物
WO2020113365A1 (zh) * 2018-12-03 2020-06-11 清华大学 一种高产脂肽的基因工程菌及其应用
EP3938504A1 (en) * 2019-03-11 2022-01-19 Basf Se Amylases and methods for making and using them
US11793196B2 (en) 2019-03-19 2023-10-24 Bayer Cropscience Lp Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health
CN111378596B (zh) * 2020-01-17 2022-07-12 合肥工业大学 一株耐酸且兼性厌氧的锰氧化细菌及其应用
CN111378597B (zh) * 2020-01-17 2022-07-12 合肥工业大学 一株可用于脱锰的锰氧化细菌及其应用

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787790B2 (ja) 1987-03-04 1995-09-27 三菱瓦斯化学株式会社 新規な遺伝子ｄｎａおよびアルカリ性プロテア−ゼの製造法
DE69033388T2 (de) 1989-08-25 2000-05-11 Henkel Research Corp., Santa Rosa Alkalisches proteolytisches enzym und verfahren zur herstellung
EP0501765A1 (en) 1991-03-01 1992-09-02 Takeda Chemical Industries, Ltd. Method of producing D-ribose
ATE189695T1 (de) 1991-06-11 2000-02-15 Genencor Int Cellulasezusammensetzungen mit einem defizit an komponenten des typs-cbh i enthaltende reinigungsmittelzusammensetzungen
US20030054534A1 (en) * 1992-07-02 2003-03-20 Novozymes A/S DNA constructs and methods of producing cellulytic enzymes
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
EP1921147B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
US5646044A (en) 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
EP2431462A3 (en) 1995-03-17 2012-05-23 Novozymes A/S Novel endoglucanases
EP0857216B1 (en) 1995-10-17 2014-09-10 AB Enzymes Oy Cellulases, the genes encoding them and uses thereof
WO1998012307A1 (en) 1996-09-17 1998-03-26 Novo Nordisk A/S Cellulase variants
DK0941349T3 (da) * 1996-11-18 2004-02-09 Novozymes Biotech Inc Fremgangsmåde til fremstilling af polypeptider i surfactinmutanter af Bacillusceller
US5958728A (en) * 1996-11-18 1999-09-28 Novo Nordiskbiotech, Inc. Methods for producing polypeptides in mutants of bacillus cells
DE19713852A1 (de) 1997-04-04 1998-10-08 Henkel Kgaa Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
AU2939100A (en) 1999-03-08 2000-09-28 Showa Sangyo Co., Ltd. Promoters
EP2011864B1 (en) 1999-03-31 2014-12-31 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
CO5271708A1 (es) 1999-12-23 2003-04-30 Upjohn Co Ensayo y metodos basados en el uso de canales de sodio como objetivos de amiloide b o de sus agregados
ATE481492T1 (de) 2000-08-04 2010-10-15 Genencor Int Mutierte trichoderma reesei egiii cellulasen, dafür kodierende dna und verfahren zu deren herstellung
DE10121463A1 (de) 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
AU2002311012A1 (en) 2001-06-06 2002-12-16 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
US20040072354A1 (en) * 2001-06-29 2004-04-15 Bedzyk Laura A. Natural promoters for gene expression and metabolic monitoring in bacillus species
DE10131441A1 (de) 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
JP2005525091A (ja) * 2001-12-21 2005-08-25 ノボザイムス バイオポリマー アクティーゼルスカブ 組換え宿主細胞におけるヒアルロナンの生成方法
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
ATE500322T1 (de) 2003-12-03 2011-03-15 Genencor Int Perhydrolase
WO2005069762A2 (en) * 2004-01-09 2005-08-04 Novozymes Inc. Bacillus licheniformis chromosome
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
CN100372937C (zh) 2005-07-21 2008-03-05 复旦大学 短小芽孢杆菌表达系统
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
DE102006022224A1 (de) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
DE102006032507A1 (de) * 2006-07-12 2008-01-17 Henkel Kgaa UV-sensitive Sicherheitsstämme für die biotechnolgische Produktion
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
US8748158B2 (en) 2007-08-10 2014-06-10 Yoshichika Kameyama Microorganism having gastric-juice promoting activity, and its secretory product
US8512982B2 (en) * 2007-12-21 2013-08-20 Danisco Us Inc. Enhanced protein production in Bacillus
JPWO2009087826A1 (ja) * 2008-01-04 2011-05-26 天野エンザイム株式会社 シュウ酸脱炭酸酵素の生産に利用される組換え発現プラスミドベクター及び組換え菌、並びに組換えシュウ酸脱炭酸酵素の生産方法
DE102008052529A1 (de) * 2008-10-21 2010-04-22 Henkel Ag & Co. Kgaa Expressionsverstärkte Nukleinsäuren

Also Published As

Publication number Publication date
DE102012201297A1 (de) 2013-08-01
CN104471066A (zh) 2015-03-25
US9725704B2 (en) 2017-08-08
WO2013113689A1 (de) 2013-08-08
RU2644199C2 (ru) 2018-02-08
DK2809816T3 (en) 2019-01-28
BR112014018534A2 (pt) 2018-09-04
CA2862731A1 (en) 2013-08-08
RU2014134728A (ru) 2016-03-27
EP2809816B9 (de) 2019-02-27
CN104471066B (zh) 2019-09-03
JP2015505463A (ja) 2015-02-23
IN2014DN07148A (enExample) 2015-04-24
MX351354B (es) 2017-10-11
MX2014009118A (es) 2015-03-03
KR20150000876A (ko) 2015-01-05
US20140370569A1 (en) 2014-12-18
BR112014018534A8 (pt) 2019-01-29
EP2809816A1 (de) 2014-12-10
PL2809816T3 (pl) 2019-04-30
EP2809816B1 (de) 2018-10-03
ES2703753T3 (es) 2019-03-12

Similar Documents

Publication Publication Date Title
US11046961B2 (en) Expression vectors with promoter and nucleic acid
JP2015505463A (ja) 発現方法
WO2016097354A1 (en) Protease variants and polynucleotides encoding same
WO2016097352A1 (en) Protease variants and polynucleotides encoding same
WO2016097357A1 (en) Protease variants and polynucleotides encoding same
WO2015091990A1 (en) Polypeptides having protease activity and polynucleotides encoding same
US20170175098A1 (en) Enzyme Variants and Polynucleotides Encoding Same
JP2017079768A (ja) 発現方法

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160126

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20161116

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20161121

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170221

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170420

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170904

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171201

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180402

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180501

R150 Certificate of patent or registration of utility model

Ref document number: 6335793

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees