IN2014DN07148A - - Google Patents

Info

Publication number
IN2014DN07148A
IN2014DN07148A IN7148DEN2014A IN2014DN07148A IN 2014DN07148 A IN2014DN07148 A IN 2014DN07148A IN 7148DEN2014 A IN7148DEN2014 A IN 7148DEN2014A IN 2014DN07148 A IN2014DN07148 A IN 2014DN07148A
Authority
IN
India
Prior art keywords
protein
expression construct
aim
microorganism
promoter
Prior art date
Application number
Other languages
English (en)
Inventor
Tobias Küppers
Victoria Steffen
Renée Charlott Eichstädt
Stefan Evers
Karl Heinz Maurer
Johannes Bongaerts
Hendrik Hellmuth
Thomas Weber
Timothy OCONNELL
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47683703&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IN2014DN07148(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Basf Se filed Critical Basf Se
Publication of IN2014DN07148A publication Critical patent/IN2014DN07148A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
IN7148DEN2014 2012-01-31 2013-01-29 IN2014DN07148A (enExample)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102012201297A DE102012201297A1 (de) 2012-01-31 2012-01-31 Expressionsverfahren
PCT/EP2013/051656 WO2013113689A1 (de) 2012-01-31 2013-01-29 Expressionsverfahren

Publications (1)

Publication Number Publication Date
IN2014DN07148A true IN2014DN07148A (enExample) 2015-04-24

Family

ID=47683703

Family Applications (1)

Application Number Title Priority Date Filing Date
IN7148DEN2014 IN2014DN07148A (enExample) 2012-01-31 2013-01-29

Country Status (15)

Country Link
US (1) US9725704B2 (enExample)
EP (1) EP2809816B9 (enExample)
JP (1) JP6335793B2 (enExample)
KR (1) KR20150000876A (enExample)
CN (1) CN104471066B (enExample)
BR (1) BR112014018534A8 (enExample)
CA (1) CA2862731A1 (enExample)
DE (1) DE102012201297A1 (enExample)
DK (1) DK2809816T3 (enExample)
ES (1) ES2703753T3 (enExample)
IN (1) IN2014DN07148A (enExample)
MX (1) MX351354B (enExample)
PL (1) PL2809816T3 (enExample)
RU (1) RU2644199C2 (enExample)
WO (1) WO2013113689A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (de) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
DE102012215107A1 (de) 2012-08-24 2014-02-27 Basf Se Festes Geschirrspülmittel mit verbesserter Proteaseleistung
CN105483100B (zh) * 2016-01-13 2019-08-13 福建农林大学 一种诱导海洋微生物发酵产κ-卡拉胶酶的组合诱导物
WO2020113365A1 (zh) * 2018-12-03 2020-06-11 清华大学 一种高产脂肽的基因工程菌及其应用
EP3938504A1 (en) * 2019-03-11 2022-01-19 Basf Se Amylases and methods for making and using them
CA3133986A1 (en) 2019-03-19 2020-09-24 Bayer Cropscience Lp Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health
CN111378597B (zh) * 2020-01-17 2022-07-12 合肥工业大学 一株可用于脱锰的锰氧化细菌及其应用
CN111378596B (zh) * 2020-01-17 2022-07-12 合肥工业大学 一株耐酸且兼性厌氧的锰氧化细菌及其应用

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787790B2 (ja) * 1987-03-04 1995-09-27 三菱瓦斯化学株式会社 新規な遺伝子ｄｎａおよびアルカリ性プロテア−ゼの製造法
ES2144990T3 (es) 1989-08-25 2000-07-01 Henkel Of America Inc Enzima proteolitica alcalina y metodo de produccion.
EP0501765A1 (en) * 1991-03-01 1992-09-02 Takeda Chemical Industries, Ltd. Method of producing D-ribose
DE69133633D1 (de) 1991-06-11 2010-07-08 Genencor Int Cellulasezusammensetzungen mit einem Defizit an Komponenten des Typs-CBH I enthaltende Reinigungsmittelzusammensetzungen
US20030054534A1 (en) * 1992-07-02 2003-03-20 Novozymes A/S DNA constructs and methods of producing cellulytic enzymes
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
EP1921147B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
WO1996029397A1 (en) 1995-03-17 1996-09-26 Novo Nordisk A/S Novel endoglucanases
EP0857216B1 (en) 1995-10-17 2014-09-10 AB Enzymes Oy Cellulases, the genes encoding them and uses thereof
EP1726644A1 (en) 1996-09-17 2006-11-29 Novozymes A/S Cellulase variants
US5958728A (en) * 1996-11-18 1999-09-28 Novo Nordiskbiotech, Inc. Methods for producing polypeptides in mutants of bacillus cells
DK0941349T3 (da) * 1996-11-18 2004-02-09 Novozymes Biotech Inc Fremgangsmåde til fremstilling af polypeptider i surfactinmutanter af Bacillusceller
DE19713852A1 (de) 1997-04-04 1998-10-08 Henkel Kgaa Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6770475B1 (en) * 1999-03-08 2004-08-03 Showa Sangyo Co., Ltd. Promoters
EP2889375B1 (en) 1999-03-31 2019-03-20 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
EP1240525A2 (en) 1999-12-23 2002-09-18 PHARMACIA & UPJOHN COMPANY Sodium channels as targets for amyloid beta
JP4907834B2 (ja) 2000-08-04 2012-04-04 ダニスコ・ユーエス・インコーポレーテッド 変異体ｅｇｉｉｉセルラーゼ、そのようなｅｇｉｉｉ組成物をコードするｄｎａ及びそれを得るための方法
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
WO2002099091A2 (en) 2001-06-06 2002-12-12 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
DE10131441A1 (de) 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
US20040072354A1 (en) * 2001-06-29 2004-04-15 Bedzyk Laura A. Natural promoters for gene expression and metabolic monitoring in bacillus species
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
ATE502115T1 (de) * 2001-12-21 2011-04-15 Novozymes Biopharma Dk As Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
ES2361838T3 (es) 2003-12-03 2011-06-22 Danisco Us Inc. Perhidrolasa.
EP3018140A3 (en) * 2004-01-09 2016-08-17 Basf Se Inactivation of glutamylpolypeptide synthesis in bacillus
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
CN100372937C (zh) * 2005-07-21 2008-03-05 复旦大学 短小芽孢杆菌表达系统
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
DE102006022224A1 (de) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
DE102006032507A1 (de) * 2006-07-12 2008-01-17 Henkel Kgaa UV-sensitive Sicherheitsstämme für die biotechnolgische Produktion
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP4695191B2 (ja) * 2007-08-10 2011-06-08 佳務 亀山 胃液促進作用を有する新規微生物及びその分泌組成物
KR20100098652A (ko) * 2007-12-21 2010-09-08 다니스코 유에스 인크. 바실루스 내에서의 향상된 단백질 생산
CA2711289A1 (en) * 2008-01-04 2009-07-16 Amano Enzyme Inc. Recombinant expression plasmid vector and recombinant strain to be used in producing oxalate decarboxylase, and method of producing recombinant oxalate decarboxylase
DE102008052529A1 (de) * 2008-10-21 2010-04-22 Henkel Ag & Co. Kgaa Expressionsverstärkte Nukleinsäuren

Also Published As

Publication number Publication date
EP2809816A1 (de) 2014-12-10
ES2703753T3 (es) 2019-03-12
JP2015505463A (ja) 2015-02-23
DE102012201297A1 (de) 2013-08-01
EP2809816B9 (de) 2019-02-27
BR112014018534A8 (pt) 2019-01-29
RU2014134728A (ru) 2016-03-27
RU2644199C2 (ru) 2018-02-08
WO2013113689A1 (de) 2013-08-08
CA2862731A1 (en) 2013-08-08
US20140370569A1 (en) 2014-12-18
KR20150000876A (ko) 2015-01-05
EP2809816B1 (de) 2018-10-03
BR112014018534A2 (pt) 2018-09-04
JP6335793B2 (ja) 2018-05-30
MX2014009118A (es) 2015-03-03
CN104471066A (zh) 2015-03-25
DK2809816T3 (en) 2019-01-28
CN104471066B (zh) 2019-09-03
PL2809816T3 (pl) 2019-04-30
US9725704B2 (en) 2017-08-08
MX351354B (es) 2017-10-11

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